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The map label for this gene is hsrA [H]

Identifier: 93006208

GI number: 93006208

Start: 1692424

End: 1693800

Strand: Direct

Name: hsrA [H]

Synonym: Pcryo_1382

Alternate gene names: 93006208

Gene position: 1692424-1693800 (Clockwise)

Preceding gene: 93006203

Following gene: 93006212

Centisome position: 55.31

GC content: 46.84

Gene sequence:

>1377_bases
ATGGTAGCCCTGACTCCAACGCAAGATAAATACCTACCCTATGTACTGGCGGTAGCGTTATTTATGCAAATTTTAGACGC
TACTATTTTAAACACGTCCCTACCACAGATGGCACAAGCATTGGGCGAATCGCCACTACAAATGCAATGGGCAGTCATCA
GCTACGCGCTTACTCTGGCGATTTTTATTCCTATTAGCGGATTTTTAGCCGATAAATATGGCACACGCCGCGTGTTCTTG
TCCGCCATTATCATATTCTGTATCGGCTCGCTATTATGTGCCGCTTCTCCGACACTAGACTTCTTAATAGGCTCACGTAT
TGTACAAGCTATCGGCGGCGCGATGATGACGCCGGTTGCACGCTTGATTTTGGTCAAGTCTTATCCGCGCAATAAGCTCT
TAACGGTGATGAACTTTGCGGTAATCCCTGCTTTGGTTGCGCCATTGGTCGGACCAGTATTGGGTGGTTATATCGTACAG
TACACCAGCTGGCACTGGATATTTTTAATCAACATACCCATGGGTGTTTTTGGTTTTATCATGGGCAAAAAACTGGTACC
AGCATTGTTTGAGGACACCAAGCGTCTAGATTGGACAGGGTTTTTGTTGTTTGCCGCTGCCGCTTGTGGTTTTACCTTGG
CGGTAGAATTTGGCGCACAAGTCGGGCGTGGGTTTTATGGGCTGATGCTTGGATTGCTGGCAAGTCTGTTGCTTGGTGCT
TATATTTGGCACGCAAAACGTCGTACAGCTCCGCTATTTCCATTGAGTTTGTTTGATATCCGTACCTTTCGCATCGGTAT
CACGGGTAATTTGTTTACCCGATTTGGCATTAGCGCTGTGCCATTTTTGCTGCCGTTGCTTTTGCAGGTGGTGTTCGAGT
ACTCTCCCTCGCAAGCTGGCTGGCTACTTGCCCCTATCGCCGTAGGTGCTATCGGCATCAAGCCTTGGGTGAGTAAGATT
ATCCAGCGTTACAGCTATCGCACGGTATTAGTCTATAACACGACTTTTATGGGTATCTTGATTATCATCTTGGCGCAATT
TAATGATGCGGCGCAGTGGATGTGGTTTATACCTATTTTAACTATCATGGGCGCATGTAACTCTATGCAGTTCAGTGCGA
TGAACACCATTACCATTGGCGACCTGCATGGTACTCAAACCAGTAGCGGCAATAGCCTGATGGCTGTCAACCAACAACTC
GCCATCAGCTTTGGTATTGCCTTTGGCGCTGCGCTGCTCACTCTGCTACGTGAGCGCCTACAAATGGATACATTGACCGC
CTTTCAAACCACCTATTGGATATTGGGTGTCTTTACCATTCTCTCAGGATTGCATTTTTTACGCTTAAAACCTGAGGATG
GTCGTGGTTTATATTAA

Upstream 100 bases:

>100_bases
TATTTTTATACTACGCATAATATAATGGCTTTGTTGAAAATTTACTGTCGATTAAGGTAAGTTTCAACGAGCCGCTGTTC
TAAGATATGGTGAAAGCAAA

Downstream 100 bases:

>100_bases
AAGTGAGTATTGAAAGGCTGTATTTGAAATATAAAAAGTAATATTTGATAAAAAATAGACCAAAAAAAGCCAGCCTATGC
TTGGGTTATGAGCAAAAAAT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MVALTPTQDKYLPYVLAVALFMQILDATILNTSLPQMAQALGESPLQMQWAVISYALTLAIFIPISGFLADKYGTRRVFL
SAIIIFCIGSLLCAASPTLDFLIGSRIVQAIGGAMMTPVARLILVKSYPRNKLLTVMNFAVIPALVAPLVGPVLGGYIVQ
YTSWHWIFLINIPMGVFGFIMGKKLVPALFEDTKRLDWTGFLLFAAAACGFTLAVEFGAQVGRGFYGLMLGLLASLLLGA
YIWHAKRRTAPLFPLSLFDIRTFRIGITGNLFTRFGISAVPFLLPLLLQVVFEYSPSQAGWLLAPIAVGAIGIKPWVSKI
IQRYSYRTVLVYNTTFMGILIIILAQFNDAAQWMWFIPILTIMGACNSMQFSAMNTITIGDLHGTQTSSGNSLMAVNQQL
AISFGIAFGAALLTLLRERLQMDTLTAFQTTYWILGVFTILSGLHFLRLKPEDGRGLY

Sequences:

>Translated_458_residues
MVALTPTQDKYLPYVLAVALFMQILDATILNTSLPQMAQALGESPLQMQWAVISYALTLAIFIPISGFLADKYGTRRVFL
SAIIIFCIGSLLCAASPTLDFLIGSRIVQAIGGAMMTPVARLILVKSYPRNKLLTVMNFAVIPALVAPLVGPVLGGYIVQ
YTSWHWIFLINIPMGVFGFIMGKKLVPALFEDTKRLDWTGFLLFAAAACGFTLAVEFGAQVGRGFYGLMLGLLASLLLGA
YIWHAKRRTAPLFPLSLFDIRTFRIGITGNLFTRFGISAVPFLLPLLLQVVFEYSPSQAGWLLAPIAVGAIGIKPWVSKI
IQRYSYRTVLVYNTTFMGILIIILAQFNDAAQWMWFIPILTIMGACNSMQFSAMNTITIGDLHGTQTSSGNSLMAVNQQL
AISFGIAFGAALLTLLRERLQMDTLTAFQTTYWILGVFTILSGLHFLRLKPEDGRGLY
>Mature_458_residues
MVALTPTQDKYLPYVLAVALFMQILDATILNTSLPQMAQALGESPLQMQWAVISYALTLAIFIPISGFLADKYGTRRVFL
SAIIIFCIGSLLCAASPTLDFLIGSRIVQAIGGAMMTPVARLILVKSYPRNKLLTVMNFAVIPALVAPLVGPVLGGYIVQ
YTSWHWIFLINIPMGVFGFIMGKKLVPALFEDTKRLDWTGFLLFAAAACGFTLAVEFGAQVGRGFYGLMLGLLASLLLGA
YIWHAKRRTAPLFPLSLFDIRTFRIGITGNLFTRFGISAVPFLLPLLLQVVFEYSPSQAGWLLAPIAVGAIGIKPWVSKI
IQRYSYRTVLVYNTTFMGILIIILAQFNDAAQWMWFIPILTIMGACNSMQFSAMNTITIGDLHGTQTSSGNSLMAVNQQL
AISFGIAFGAALLTLLRERLQMDTLTAFQTTYWILGVFTILSGLHFLRLKPEDGRGLY

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=451, Percent_Identity=47.450110864745, Blast_Score=393, Evalue=1e-110,
Organism=Escherichia coli, GI1788392, Length=457, Percent_Identity=40.4814004376368, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1789042, Length=421, Percent_Identity=28.2660332541568, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788710, Length=323, Percent_Identity=30.6501547987616, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI87081983, Length=314, Percent_Identity=27.0700636942675, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1788509, Length=136, Percent_Identity=28.6764705882353, Blast_Score=65, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=138, Percent_Identity=34.7826086956522, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6321663, Length=153, Percent_Identity=32.0261437908497, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6322958, Length=169, Percent_Identity=31.3609467455621, Blast_Score=77, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50345; Mature: 50345

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVALTPTQDKYLPYVLAVALFMQILDATILNTSLPQMAQALGESPLQMQWAVISYALTLA
CEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
IFIPISGFLADKYGTRRVFLSAIIIFCIGSLLCAASPTLDFLIGSRIVQAIGGAMMTPVA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHH
RLILVKSYPRNKLLTVMNFAVIPALVAPLVGPVLGGYIVQYTSWHWIFLINIPMGVFGFI
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEEEEEECCHHHHHHH
MGKKLVPALFEDTKRLDWTGFLLFAAAACGFTLAVEFGAQVGRGFYGLMLGLLASLLLGA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
YIWHAKRRTAPLFPLSLFDIRTFRIGITGNLFTRFGISAVPFLLPLLLQVVFEYSPSQAG
HHHHHHCCCCCCCCCHHHEEEEEEEEECCCHHHHHCHHHHHHHHHHHHHHHHHCCCCCCC
WLLAPIAVGAIGIKPWVSKIIQRYSYRTVLVYNTTFMGILIIILAQFNDAAQWMWFIPIL
EEHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHH
TIMGACNSMQFSAMNTITIGDLHGTQTSSGNSLMAVNQQLAISFGIAFGAALLTLLRERL
HHHCCCCCCEECCCCEEEEECCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHH
QMDTLTAFQTTYWILGVFTILSGLHFLRLKPEDGRGLY
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
>Mature Secondary Structure
MVALTPTQDKYLPYVLAVALFMQILDATILNTSLPQMAQALGESPLQMQWAVISYALTLA
CEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
IFIPISGFLADKYGTRRVFLSAIIIFCIGSLLCAASPTLDFLIGSRIVQAIGGAMMTPVA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHH
RLILVKSYPRNKLLTVMNFAVIPALVAPLVGPVLGGYIVQYTSWHWIFLINIPMGVFGFI
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEEEEEECCHHHHHHH
MGKKLVPALFEDTKRLDWTGFLLFAAAACGFTLAVEFGAQVGRGFYGLMLGLLASLLLGA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
YIWHAKRRTAPLFPLSLFDIRTFRIGITGNLFTRFGISAVPFLLPLLLQVVFEYSPSQAG
HHHHHHCCCCCCCCCHHHEEEEEEEEECCCHHHHHCHHHHHHHHHHHHHHHHHCCCCCCC
WLLAPIAVGAIGIKPWVSKIIQRYSYRTVLVYNTTFMGILIIILAQFNDAAQWMWFIPIL
EEHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHH
TIMGACNSMQFSAMNTITIGDLHGTQTSSGNSLMAVNQQLAISFGIAFGAALLTLLRERL
HHHCCCCCCEECCCCEEEEECCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHH
QMDTLTAFQTTYWILGVFTILSGLHFLRLKPEDGRGLY
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]