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The map label for this gene is nirB [H]

Identifier: 93006147

GI number: 93006147

Start: 1609584

End: 1612193

Strand: Reverse

Name: nirB [H]

Synonym: Pcryo_1319

Alternate gene names: 93006147

Gene position: 1612193-1609584 (Counterclockwise)

Preceding gene: 93006153

Following gene: 93006146

Centisome position: 52.69

GC content: 47.2

Gene sequence:

>2610_bases
ATGAGTAGCACTATAGTGAGTAAGGCAGGTAAAGCTAAGTTGGTGGTGATTGGTAACGGTATGGTCGGGCACCATTTCGT
TGAGCGAGCCATAGAAAAAGGGTTACATGAGCAGTTTGAGATTCATGTGTTTGCTGAAGAAGACCGTCCCGCTTACGATC
GGGTATATTTATCGAGCTACTTTGAACATAAAGATGTTAGTAAGCTCAACTTGGTTGATTTAAGCGCCTATGCATCTGCC
CAGATTCAATTGCATCTGAACCAGCGTATTGTAGATATTGACACCACCAATCAATACATCATCACTGAAGCTGGCGAGCG
GATTGAATATGGTGAGCTGGTGCTGGCGACAGGCTCCTATCCATTTGTACCGCCGATTCCAGGGCGTGAGCATGCACACT
GTCATGTATATCGCACCATTGCTGATTTGGATGACATTCTTGCCATCGCTGAGCAACCAAAAGTAAAAAGAGGCGTAGTT
GTGGGCGGAGGACTACTCGGGCTTGAAGCAGCAAAAGCACTGGTCACCTTAGGACTAGAGACTTATGTGGTAGAGTTTGC
TCCGCAATTGATGGGCGTACAGCTTGATGCGGCTGGCGGCGATATTTTAAAACGAAAAATTGAGGCGCTTGGCGTCAAAG
TATTAACTGGTAAGAATACCCAAGCCATTATTGATAATAGCGACGCCATACATAAAAACCTGAGCACCTTTAATGAGAAT
CCGTGCCAGCTGACCATGCAATTCACCGATGATACCTCGCTTGACACCGATATGATCGTCTTTAGTGCAGGTATTCGTCC
GCAAGATGGCATTATCAAAAACAATCCTGAGTGCGGTATTGAGATAGGTGCACGAGGTGGTATTTTAATTGACGAACAAT
GCCGCACCAATGCTGCGCATGTCTACGCTATTGGCGAATGCGCGGTGTGGGATAACAAGATATTTGGCTTAGTAGCACCA
GGCTACAGCATGGCGAGTATTTGCGCGGCACAAATCACAGGAGATCAGTCGCAAACCTTTACGGGCGCTGATATGAGCAC
CAAGCTCAAACTGATGGGCGTCGATGTTGGTAGTATTGGCGACGCCCATGGCAACAGCGCGGACAGCTTGAGCTATTTGT
ACCAGAACCCTGCTGAGGGCATTTATAAGAAATTGGTCACAACGCCTGATAACAAACGCTTGCTTGGCGCGGTGTTGGTC
GGTGATACCGAAGACTATAATAATCTATTACAGCTATATCTCAACGACATCGACCTACCTGCCCACCCTGAAAGCCTCAT
ACTACCCAACGTTGAAAAACCGAAAATGGGTATAGAAAATTTACCAGATAGTGCAACGATTTGCTCATGCTACAACGTCA
CCAAAGGGGCTATTTGCGCGGCGGTTGAAAACGGTGCGTACTCCATTGCCGAAGTGAAGTCTTGTACTAGTGCAGGTACA
GGATGTGGTGGCTGTGCGCCTATGGTGAAAGACACTTTAAGCTACCAGTTAACGACCCTTGGGTTTGAGGTCAATAACGA
CTTATGTGAGCACTTTGCCTATACCCGTCAAGAGCTCTATAGCTTGATTCGTGTCCATGGTATTAGAACCTTTGATGCTC
TGTTGGATGAGCATGGTAACGGTCATGGCTGTGAGATTTGCAAACCTACGGTCGCCTCCATCTTGGCCTCATGCTGGAAT
GATTATGTGCTCAAGCCTGAACTGACACCGTTGCAAGATAGCAATGACTATTATCTGGGCAACATCCAAAAAGACGGCAC
CTATTCTGTCGTACCTCGTGTGCCGGGTGGTGAAATCACCGCCGATAAGCTGATCATCCTCGGACAAGTCGCCAAAGAGT
TTAATTTGTATACCAAAATCACAGGTGGGCAACGCGTGGATCTATTTGGGGCACGTTTAGACCAATTGCCTGATATCTGG
ACACAATTGGTGCAAGCTGGCTTTGAGACTGGTCACGCTTATGGTAAGTCGTTGCGTACTGTTAAATCGTGCGTGGGTAA
CAACTGGTGTCGCTACGGTGTCGATGACAGTGTCGGCTTGGCACTCACCTTAGAGGATCGTTATAAAGGAGTACGTTCAC
CGCATAAGATAAAGATGGGCGTGTCCGGATGTACACGCGAGTGTGCAGAGGCACAAAGCAAGGACTTTGGCATTATCGCC
ACTGAGAATGGTTGGAATTTATTTGTCTGTGGTAATGGTGGTATGACCCCGCGTCATGGTGAGTTGTTTGCCACCGACCT
TGATAGCGACACGGTCATCAAATACATCGACCGTATTATCATGTTTTATATCAAAACAGCAGATAAACTCCAGCGTACCT
CAAAATGGCGTGAGAGCTTAGATGGCGGATTGGAATATCTACAAGCGGTCATTATTGAAGACAGTTTAGGCATCGCTGAT
GAGCTAGAGGCGCAGATGCAACTGCTGATCGACAACTATGTGTGTGAGTGGAAAGCAACTATAACCGATAGCGAAAAGCT
CAAACGTTTCCGTCACTTCGTCAATAGTGAGCAAGGGGATGACAATGTGGTTTTTGTCACCGAGCGCGAGCAGATCCGCC
CTGCGACCGATAGTGAAAAAGCTAAAATCTTAGTGACTGAGCTGGCTTAA

Upstream 100 bases:

>100_bases
GCTTAAAGATATGGCGCTATATAACGGTGCTTGGCACAGTTATTGAAGATGTAATTACTGTAAGCACTATTTAGACAAAC
CCATGACTAAGGATAATTAC

Downstream 100 bases:

>100_bases
TCAACGTCTAACACTTTCCGATCATATACGAATAAGGATCACCCAATGAACCAGTATACCGTTTGCAGATTAGAAGACAT
ACTCCCTGAGACTGGTGTTT

Product: nitrite reductase (NAD(P)H) large subunit, NirB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 869; Mature: 868

Protein sequence:

>869_residues
MSSTIVSKAGKAKLVVIGNGMVGHHFVERAIEKGLHEQFEIHVFAEEDRPAYDRVYLSSYFEHKDVSKLNLVDLSAYASA
QIQLHLNQRIVDIDTTNQYIITEAGERIEYGELVLATGSYPFVPPIPGREHAHCHVYRTIADLDDILAIAEQPKVKRGVV
VGGGLLGLEAAKALVTLGLETYVVEFAPQLMGVQLDAAGGDILKRKIEALGVKVLTGKNTQAIIDNSDAIHKNLSTFNEN
PCQLTMQFTDDTSLDTDMIVFSAGIRPQDGIIKNNPECGIEIGARGGILIDEQCRTNAAHVYAIGECAVWDNKIFGLVAP
GYSMASICAAQITGDQSQTFTGADMSTKLKLMGVDVGSIGDAHGNSADSLSYLYQNPAEGIYKKLVTTPDNKRLLGAVLV
GDTEDYNNLLQLYLNDIDLPAHPESLILPNVEKPKMGIENLPDSATICSCYNVTKGAICAAVENGAYSIAEVKSCTSAGT
GCGGCAPMVKDTLSYQLTTLGFEVNNDLCEHFAYTRQELYSLIRVHGIRTFDALLDEHGNGHGCEICKPTVASILASCWN
DYVLKPELTPLQDSNDYYLGNIQKDGTYSVVPRVPGGEITADKLIILGQVAKEFNLYTKITGGQRVDLFGARLDQLPDIW
TQLVQAGFETGHAYGKSLRTVKSCVGNNWCRYGVDDSVGLALTLEDRYKGVRSPHKIKMGVSGCTRECAEAQSKDFGIIA
TENGWNLFVCGNGGMTPRHGELFATDLDSDTVIKYIDRIIMFYIKTADKLQRTSKWRESLDGGLEYLQAVIIEDSLGIAD
ELEAQMQLLIDNYVCEWKATITDSEKLKRFRHFVNSEQGDDNVVFVTEREQIRPATDSEKAKILVTELA

Sequences:

>Translated_869_residues
MSSTIVSKAGKAKLVVIGNGMVGHHFVERAIEKGLHEQFEIHVFAEEDRPAYDRVYLSSYFEHKDVSKLNLVDLSAYASA
QIQLHLNQRIVDIDTTNQYIITEAGERIEYGELVLATGSYPFVPPIPGREHAHCHVYRTIADLDDILAIAEQPKVKRGVV
VGGGLLGLEAAKALVTLGLETYVVEFAPQLMGVQLDAAGGDILKRKIEALGVKVLTGKNTQAIIDNSDAIHKNLSTFNEN
PCQLTMQFTDDTSLDTDMIVFSAGIRPQDGIIKNNPECGIEIGARGGILIDEQCRTNAAHVYAIGECAVWDNKIFGLVAP
GYSMASICAAQITGDQSQTFTGADMSTKLKLMGVDVGSIGDAHGNSADSLSYLYQNPAEGIYKKLVTTPDNKRLLGAVLV
GDTEDYNNLLQLYLNDIDLPAHPESLILPNVEKPKMGIENLPDSATICSCYNVTKGAICAAVENGAYSIAEVKSCTSAGT
GCGGCAPMVKDTLSYQLTTLGFEVNNDLCEHFAYTRQELYSLIRVHGIRTFDALLDEHGNGHGCEICKPTVASILASCWN
DYVLKPELTPLQDSNDYYLGNIQKDGTYSVVPRVPGGEITADKLIILGQVAKEFNLYTKITGGQRVDLFGARLDQLPDIW
TQLVQAGFETGHAYGKSLRTVKSCVGNNWCRYGVDDSVGLALTLEDRYKGVRSPHKIKMGVSGCTRECAEAQSKDFGIIA
TENGWNLFVCGNGGMTPRHGELFATDLDSDTVIKYIDRIIMFYIKTADKLQRTSKWRESLDGGLEYLQAVIIEDSLGIAD
ELEAQMQLLIDNYVCEWKATITDSEKLKRFRHFVNSEQGDDNVVFVTEREQIRPATDSEKAKILVTELA
>Mature_868_residues
SSTIVSKAGKAKLVVIGNGMVGHHFVERAIEKGLHEQFEIHVFAEEDRPAYDRVYLSSYFEHKDVSKLNLVDLSAYASAQ
IQLHLNQRIVDIDTTNQYIITEAGERIEYGELVLATGSYPFVPPIPGREHAHCHVYRTIADLDDILAIAEQPKVKRGVVV
GGGLLGLEAAKALVTLGLETYVVEFAPQLMGVQLDAAGGDILKRKIEALGVKVLTGKNTQAIIDNSDAIHKNLSTFNENP
CQLTMQFTDDTSLDTDMIVFSAGIRPQDGIIKNNPECGIEIGARGGILIDEQCRTNAAHVYAIGECAVWDNKIFGLVAPG
YSMASICAAQITGDQSQTFTGADMSTKLKLMGVDVGSIGDAHGNSADSLSYLYQNPAEGIYKKLVTTPDNKRLLGAVLVG
DTEDYNNLLQLYLNDIDLPAHPESLILPNVEKPKMGIENLPDSATICSCYNVTKGAICAAVENGAYSIAEVKSCTSAGTG
CGGCAPMVKDTLSYQLTTLGFEVNNDLCEHFAYTRQELYSLIRVHGIRTFDALLDEHGNGHGCEICKPTVASILASCWND
YVLKPELTPLQDSNDYYLGNIQKDGTYSVVPRVPGGEITADKLIILGQVAKEFNLYTKITGGQRVDLFGARLDQLPDIWT
QLVQAGFETGHAYGKSLRTVKSCVGNNWCRYGVDDSVGLALTLEDRYKGVRSPHKIKMGVSGCTRECAEAQSKDFGIIAT
ENGWNLFVCGNGGMTPRHGELFATDLDSDTVIKYIDRIIMFYIKTADKLQRTSKWRESLDGGLEYLQAVIIEDSLGIADE
LEAQMQLLIDNYVCEWKATITDSEKLKRFRHFVNSEQGDDNVVFVTEREQIRPATDSEKAKILVTELA

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789765, Length=860, Percent_Identity=56.2790697674419, Blast_Score=986, Evalue=0.0,
Organism=Escherichia coli, GI1789065, Length=330, Percent_Identity=22.1212121212121, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1788892, Length=271, Percent_Identity=22.5092250922509, Blast_Score=71, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17559934, Length=306, Percent_Identity=24.8366013071895, Blast_Score=82, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281359715, Length=297, Percent_Identity=25.5892255892256, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281359713, Length=297, Percent_Identity=25.5892255892256, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24639250, Length=297, Percent_Identity=25.5892255892256, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI18543267, Length=297, Percent_Identity=25.5892255892256, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24639252, Length=297, Percent_Identity=25.5892255892256, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24639257, Length=297, Percent_Identity=25.5892255892256, Blast_Score=69, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 95096; Mature: 94965

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00022 EGF_1 ; PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTIVSKAGKAKLVVIGNGMVGHHFVERAIEKGLHEQFEIHVFAEEDRPAYDRVYLSSY
CCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHH
FEHKDVSKLNLVDLSAYASAQIQLHLNQRIVDIDTTNQYIITEAGERIEYGELVLATGSY
HCCCCCCEEEEEEEECCCEEEEEEEECCEEEEEECCCCEEEECCCCCEECCCEEEEECCC
PFVPPIPGREHAHCHVYRTIADLDDILAIAEQPKVKRGVVVGGGLLGLEAAKALVTLGLE
CCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHH
TYVVEFAPQLMGVQLDAAGGDILKRKIEALGVKVLTGKNTQAIIDNSDAIHKNLSTFNEN
HHHHHHHHHHHCEEEECCCHHHHHHHHHHCCEEEEECCCCEEEECCCHHHHHHHHHCCCC
PCQLTMQFTDDTSLDTDMIVFSAGIRPQDGIIKNNPECGIEIGARGGILIDEQCRTNAAH
CEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEECCCCCEEECCCCCCCCEE
VYAIGECAVWDNKIFGLVAPGYSMASICAAQITGDQSQTFTGADMSTKLKLMGVDVGSIG
EEEEEEEEEECCCEEEEECCCCHHHHHHHHHCCCCCCCEEECCCCCCEEEEEEECCCCCC
DAHGNSADSLSYLYQNPAEGIYKKLVTTPDNKRLLGAVLVGDTEDYNNLLQLYLNDIDLP
CCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCC
AHPESLILPNVEKPKMGIENLPDSATICSCYNVTKGAICAAVENGAYSIAEVKSCTSAGT
CCCCCEECCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCEEHHHHHHHHCCCC
GCGGCAPMVKDTLSYQLTTLGFEVNNDLCEHFAYTRQELYSLIRVHGIRTFDALLDEHGN
CCCCCHHHHHHHHCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
GHGCEICKPTVASILASCWNDYVLKPELTPLQDSNDYYLGNIQKDGTYSVVPRVPGGEIT
CCCCCCCCHHHHHHHHHHHCCEEECCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCC
ADKLIILGQVAKEFNLYTKITGGQRVDLFGARLDQLPDIWTQLVQAGFETGHAYGKSLRT
HHHEEEEEHHHHHCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKSCVGNNWCRYGVDDSVGLALTLEDRYKGVRSPHKIKMGVSGCTRECAEAQSKDFGIIA
HHHHHCCCCEECCCCCCCEEEEEEHHHHCCCCCCCEEEECCCHHHHHHHHHCCCCCEEEE
TENGWNLFVCGNGGMTPRHGELFATDLDSDTVIKYIDRIIMFYIKTADKLQRTSKWRESL
ECCCCEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DGGLEYLQAVIIEDSLGIADELEAQMQLLIDNYVCEWKATITDSEKLKRFRHFVNSEQGD
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHCCCCCC
DNVVFVTEREQIRPATDSEKAKILVTELA
CCEEEEECHHHCCCCCCCCCCEEEEEECC
>Mature Secondary Structure 
SSTIVSKAGKAKLVVIGNGMVGHHFVERAIEKGLHEQFEIHVFAEEDRPAYDRVYLSSY
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHH
FEHKDVSKLNLVDLSAYASAQIQLHLNQRIVDIDTTNQYIITEAGERIEYGELVLATGSY
HCCCCCCEEEEEEEECCCEEEEEEEECCEEEEEECCCCEEEECCCCCEECCCEEEEECCC
PFVPPIPGREHAHCHVYRTIADLDDILAIAEQPKVKRGVVVGGGLLGLEAAKALVTLGLE
CCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHH
TYVVEFAPQLMGVQLDAAGGDILKRKIEALGVKVLTGKNTQAIIDNSDAIHKNLSTFNEN
HHHHHHHHHHHCEEEECCCHHHHHHHHHHCCEEEEECCCCEEEECCCHHHHHHHHHCCCC
PCQLTMQFTDDTSLDTDMIVFSAGIRPQDGIIKNNPECGIEIGARGGILIDEQCRTNAAH
CEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEECCCCCEEECCCCCCCCEE
VYAIGECAVWDNKIFGLVAPGYSMASICAAQITGDQSQTFTGADMSTKLKLMGVDVGSIG
EEEEEEEEEECCCEEEEECCCCHHHHHHHHHCCCCCCCEEECCCCCCEEEEEEECCCCCC
DAHGNSADSLSYLYQNPAEGIYKKLVTTPDNKRLLGAVLVGDTEDYNNLLQLYLNDIDLP
CCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCC
AHPESLILPNVEKPKMGIENLPDSATICSCYNVTKGAICAAVENGAYSIAEVKSCTSAGT
CCCCCEECCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCEEHHHHHHHHCCCC
GCGGCAPMVKDTLSYQLTTLGFEVNNDLCEHFAYTRQELYSLIRVHGIRTFDALLDEHGN
CCCCCHHHHHHHHCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
GHGCEICKPTVASILASCWNDYVLKPELTPLQDSNDYYLGNIQKDGTYSVVPRVPGGEIT
CCCCCCCCHHHHHHHHHHHCCEEECCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCC
ADKLIILGQVAKEFNLYTKITGGQRVDLFGARLDQLPDIWTQLVQAGFETGHAYGKSLRT
HHHEEEEEHHHHHCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKSCVGNNWCRYGVDDSVGLALTLEDRYKGVRSPHKIKMGVSGCTRECAEAQSKDFGIIA
HHHHHCCCCEECCCCCCCEEEEEEHHHHCCCCCCCEEEECCCHHHHHHHHHCCCCCEEEE
TENGWNLFVCGNGGMTPRHGELFATDLDSDTVIKYIDRIIMFYIKTADKLQRTSKWRESL
ECCCCEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DGGLEYLQAVIIEDSLGIADELEAQMQLLIDNYVCEWKATITDSEKLKRFRHFVNSEQGD
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHCCCCCC
DNVVFVTEREQIRPATDSEKAKILVTELA
CCEEEEECHHHCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2543955; 2200672; 9278503; 2445993 [H]