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The map label for this gene is nirD [H]

Identifier: 93006146

GI number: 93006146

Start: 1609131

End: 1609538

Strand: Reverse

Name: nirD [H]

Synonym: Pcryo_1318

Alternate gene names: 93006146

Gene position: 1609538-1609131 (Counterclockwise)

Preceding gene: 93006147

Following gene: 93006145

Centisome position: 52.6

GC content: 42.65

Gene sequence:

>408_bases
ATGAACCAGTATACCGTTTGCAGATTAGAAGACATACTCCCTGAGACTGGTGTTTGCGCACTGATAGAGGGTAAACAAGT
GGCTATCTTTCGCACTAAGGACGCTAAGCTGTTCGCGCTTGATAACTATGACCCGTTTAGTCAGGCCAATGTATTATCTA
GAGGGTTAATTGGAGGCACTACCGTGACCAACGATGCTGGCACTGATGAAGCGGTACTGTATGTGGCCTCACCTATTTAT
AAACAGAGATTTAACCTTGCCACCGGTCAATGCTTGGATGATACAAGTGTCACGCTTGCTACTTACCATGTTGAGCTTGA
TACCAGTCTTGAAGGTAATAATGTGGTGATAAAATATGATCAGTTAGAAGCGTCATCTACAACACTATTAGAAATGGAAG
TTGTTTAA

Upstream 100 bases:

>100_bases
CCGCCCTGCGACCGATAGTGAAAAAGCTAAAATCTTAGTGACTGAGCTGGCTTAATCAACGTCTAACACTTTCCGATCAT
ATACGAATAAGGATCACCCA

Downstream 100 bases:

>100_bases
GGCGTAGCCGTCTTTTTAGCAAATTGAAACATGTAATTTACATTAAGATACTGAATAAAAAATTGTACTGGCATAATATT
GGCGGTAAATATATTTCACT

Product: nitrite reductase (NAD(P)H) large subunit, NirD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 135; Mature: 135

Protein sequence:

>135_residues
MNQYTVCRLEDILPETGVCALIEGKQVAIFRTKDAKLFALDNYDPFSQANVLSRGLIGGTTVTNDAGTDEAVLYVASPIY
KQRFNLATGQCLDDTSVTLATYHVELDTSLEGNNVVIKYDQLEASSTTLLEMEVV

Sequences:

>Translated_135_residues
MNQYTVCRLEDILPETGVCALIEGKQVAIFRTKDAKLFALDNYDPFSQANVLSRGLIGGTTVTNDAGTDEAVLYVASPIY
KQRFNLATGQCLDDTSVTLATYHVELDTSLEGNNVVIKYDQLEASSTTLLEMEVV
>Mature_135_residues
MNQYTVCRLEDILPETGVCALIEGKQVAIFRTKDAKLFALDNYDPFSQANVLSRGLIGGTTVTNDAGTDEAVLYVASPIY
KQRFNLATGQCLDDTSVTLATYHVELDTSLEGNNVVIKYDQLEASSTTLLEMEVV

Specific function: Required for activity of the reductase [H]

COG id: COG2146

COG function: function code PR; Ferredoxin subunits of nitrite reductase and ring-hydroxylating dioxygenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis nasE [H]

Homologues:

Organism=Escherichia coli, GI1789766, Length=104, Percent_Identity=45.1923076923077, Blast_Score=96, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012748
- InterPro:   IPR017881
- InterPro:   IPR017941 [H]

Pfam domain/function: NA

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 14774; Mature: 14774

Theoretical pI: Translated: 4.06; Mature: 4.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQYTVCRLEDILPETGVCALIEGKQVAIFRTKDAKLFALDNYDPFSQANVLSRGLIGGT
CCCEEEEEEHHCCCCCCEEEEECCCEEEEEEECCCEEEEECCCCCCCHHHHHHCCCCCCC
TVTNDAGTDEAVLYVASPIYKQRFNLATGQCLDDTSVTLATYHVELDTSLEGNNVVIKYD
EEECCCCCCCEEEEEECCHHHHHCCCCCCCEECCCCEEEEEEEEEEECCCCCCEEEEEEE
QLEASSTTLLEMEVV
CCCCCCEEEEEEEEC
>Mature Secondary Structure
MNQYTVCRLEDILPETGVCALIEGKQVAIFRTKDAKLFALDNYDPFSQANVLSRGLIGGT
CCCEEEEEEHHCCCCCCEEEEECCCEEEEEEECCCEEEEECCCCCCCHHHHHHCCCCCCC
TVTNDAGTDEAVLYVASPIYKQRFNLATGQCLDDTSVTLATYHVELDTSLEGNNVVIKYD
EEECCCCCCCEEEEEECCHHHHHCCCCCCCEECCCCEEEEEEEEEEECCCCCCEEEEEEE
QLEASSTTLLEMEVV
CCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]