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The map label for this gene is pcaJ [H]

Identifier: 93006089

GI number: 93006089

Start: 1532448

End: 1533113

Strand: Reverse

Name: pcaJ [H]

Synonym: Pcryo_1261

Alternate gene names: 93006089

Gene position: 1533113-1532448 (Counterclockwise)

Preceding gene: 93006090

Following gene: 93006088

Centisome position: 50.1

GC content: 47.3

Gene sequence:

>666_bases
ATGAGCCATACTTCCTACACCGTTTTGACCCGCGATCAAATCGCTGAGCGCGCTGCACAAGATATCCCAGATGGTGCCTA
TGTCAATTTAGGCATCGGACTACCAACTAAAATTGCAAAATACCTACCGGATGATAAAGATGTATTTTTGCATTCAGAAA
ACGGTTTACTAGCTTTTGGACCCCCACCTGCTAAAGGTGAAGAAGATCCCGAGCTGATTAATGCCGGAAAAGAGTACGTC
ACCATGCTCGATGGCGGTAGCTTCTTTCATCATGGCGACTCCTTTGCCATGATGCGCGGTGGTCATATCGATATTTGCGT
ATTGGGTGCGTTTCAAGTGGCTGCCAATGGCGACTTAGCCAACTGGAGCACGGGTGCGCCAGATGCAATACCTGCGGTTG
GCGGCGCTATGGACTTAGCCGTCGGTGCCAAAAAAGTCTTTGTGATGACCAATCACACCACCAAAACTGGCGACCCTAAA
ATCGTTAGCAAGCTTACCTACCCCGTGACTGGCAAAAACTGTGTTGATCGTATTTATACTGACTTATGTGTCATTGATGT
CACTGATAAAGGTTTGGCCGTCACTGAAATGGTCGATGGCTTAAGCTTTGCGGACTTACAAGCATTGACGGGTGCGACGC
TAATTGACGCTACAAATTCAAAATAA

Upstream 100 bases:

>100_bases
TTACACCGGGTATCTTTGTGCAGCATGTGGTGCAAATTCAGCCTGCTAGCACAGCAACTACAGCCACCGCTTAATACTGC
CAACTATAAGGAGCGTCATC

Downstream 100 bases:

>100_bases
TAAAAAGGACAAGGATGATGACTGATTCAATCACTGGTTTAAATAATGCTTATATTATTGATGCCATTCGCACCCCTTTT
GGTCGCTACGGCGGCGGTCT

Product: 3-oxoacid CoA-transferase subunit B

Products: NA

Alternate protein names: Beta-ketoadipate:succinyl-CoA transferase subunit B [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MSHTSYTVLTRDQIAERAAQDIPDGAYVNLGIGLPTKIAKYLPDDKDVFLHSENGLLAFGPPPAKGEEDPELINAGKEYV
TMLDGGSFFHHGDSFAMMRGGHIDICVLGAFQVAANGDLANWSTGAPDAIPAVGGAMDLAVGAKKVFVMTNHTTKTGDPK
IVSKLTYPVTGKNCVDRIYTDLCVIDVTDKGLAVTEMVDGLSFADLQALTGATLIDATNSK

Sequences:

>Translated_221_residues
MSHTSYTVLTRDQIAERAAQDIPDGAYVNLGIGLPTKIAKYLPDDKDVFLHSENGLLAFGPPPAKGEEDPELINAGKEYV
TMLDGGSFFHHGDSFAMMRGGHIDICVLGAFQVAANGDLANWSTGAPDAIPAVGGAMDLAVGAKKVFVMTNHTTKTGDPK
IVSKLTYPVTGKNCVDRIYTDLCVIDVTDKGLAVTEMVDGLSFADLQALTGATLIDATNSK
>Mature_220_residues
SHTSYTVLTRDQIAERAAQDIPDGAYVNLGIGLPTKIAKYLPDDKDVFLHSENGLLAFGPPPAKGEEDPELINAGKEYVT
MLDGGSFFHHGDSFAMMRGGHIDICVLGAFQVAANGDLANWSTGAPDAIPAVGGAMDLAVGAKKVFVMTNHTTKTGDPKI
VSKLTYPVTGKNCVDRIYTDLCVIDVTDKGLAVTEMVDGLSFADLQALTGATLIDATNSK

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=203, Percent_Identity=48.7684729064039, Blast_Score=185, Evalue=3e-47,
Organism=Homo sapiens, GI11545841, Length=213, Percent_Identity=46.9483568075117, Blast_Score=185, Evalue=4e-47,
Organism=Escherichia coli, GI1788552, Length=202, Percent_Identity=42.5742574257426, Blast_Score=165, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17531517, Length=221, Percent_Identity=46.1538461538462, Blast_Score=180, Evalue=6e-46,
Organism=Drosophila melanogaster, GI21358325, Length=213, Percent_Identity=45.0704225352113, Blast_Score=180, Evalue=8e-46,
Organism=Drosophila melanogaster, GI24655794, Length=213, Percent_Identity=45.0704225352113, Blast_Score=179, Evalue=9e-46,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.6 [H]

Molecular weight: Translated: 23332; Mature: 23201

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHTSYTVLTRDQIAERAAQDIPDGAYVNLGIGLPTKIAKYLPDDKDVFLHSENGLLAFG
CCCCEEEEEEHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCCCEEEEECCCCEEEEC
PPPAKGEEDPELINAGKEYVTMLDGGSFFHHGDSFAMMRGGHIDICVLGAFQVAANGDLA
CCCCCCCCCHHHHHCCCEEEEEEECCCEEECCCCEEEEECCCEEEEEEEEEEEECCCCCC
NWSTGAPDAIPAVGGAMDLAVGAKKVFVMTNHTTKTGDPKIVSKLTYPVTGKNCVDRIYT
CCCCCCCCCHHHCCCHHHEECCCEEEEEEECCCCCCCCCCEEHEEECCCCCHHHHHHHHH
DLCVIDVTDKGLAVTEMVDGLSFADLQALTGATLIDATNSK
CEEEEEECCCCEEHHHHHCCCCHHHHHHHCCCEEEECCCCC
>Mature Secondary Structure 
SHTSYTVLTRDQIAERAAQDIPDGAYVNLGIGLPTKIAKYLPDDKDVFLHSENGLLAFG
CCCEEEEEEHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCCCEEEEECCCCEEEEC
PPPAKGEEDPELINAGKEYVTMLDGGSFFHHGDSFAMMRGGHIDICVLGAFQVAANGDLA
CCCCCCCCCHHHHHCCCEEEEEEECCCEEECCCCEEEEECCCEEEEEEEEEEEECCCCCC
NWSTGAPDAIPAVGGAMDLAVGAKKVFVMTNHTTKTGDPKIVSKLTYPVTGKNCVDRIYT
CCCCCCCCCHHHCCCHHHEECCCEEEEEEECCCCCCCCCCEEHEEECCCCCHHHHHHHHH
DLCVIDVTDKGLAVTEMVDGLSFADLQALTGATLIDATNSK
CEEEEEECCCCEEHHHHHCCCCHHHHHHHCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8063101; 8125318 [H]