Click here to switch to the map view.

The map label for this gene is livM [H]

Identifier: 93005820

GI number: 93005820

Start: 1181735

End: 1182805

Strand: Reverse

Name: livM [H]

Synonym: Pcryo_0992

Alternate gene names: 93005820

Gene position: 1182805-1181735 (Counterclockwise)

Preceding gene: 93005821

Following gene: 93005819

Centisome position: 38.66

GC content: 46.13

Gene sequence:

>1071_bases
ATGCGTTTGACTCAATTATTATCTGATTCCCCACGTAATGCCATTTTGATTGGTTTGTGTTTTTTAGTGCTACTGATATT
GCCTTGGCTCCATCTACTACCTGAAACCTCTAGTTTTCACCTGTCTGCTTACTGGATAACTTTGATTGGTAAAATAATGG
CGCTCGCCATGGTAGCACTGGCTTTAGACTTGGTCTGGGGCTATGCTGGCATCCTAAGCTTAGGTCATGGTCTTTATTTT
GCGCTAGGAGGTTATGCCTTTGGCATGTACTTGACGCGTGAAACTGCTGGCACGGGCTTGCCAGATTTTATGCGTTTCTT
AAGCTGGACCGAGCTGCCGTGGTATTGGGCAGGCACTCAACATTTCTGGTGGGCGATGGCTCTAGTGGTATTAGTTCCCG
GTCTGGTCGCTTTCATCTTTGGCTATTTTGCTTTTCGCTCAAAAATCAAAGGGGTTTATTTTTCGATTATTACTCAAGCC
ATGACCTATGCGGCAGCCTTATTATTCTTCCGTAACGAAACAGGCTTCGGTGGTAACAATGGCTTCACAGGTTTTACCAC
GTTATTAGGCTATGACATCACTGCCTCTTCAACGAGAGCTGTGCTGTGTTTTACGACTGCTTTGGCGCTGCTACTGTCCT
ATCTTGGACTACGCTATCTGATGAGCCAGCCTTATGGGCGCGTGCTCGGTGCCATCCGTGATAGTGAAAATCGCTTGCAG
TATTTAGGCTATCGCACGCTTTGGTACAAGCTTTCTGCTTGGGTATTGTCTGCAGTCATTGCTGGTATCGCTGGTGCTCT
ATATGTACCGCAAGTTGGCATCATTAATCCGGGCGAGATGAACCCCGTCAACTCTATCGAGATGGCAGTATGGGTAGCTG
CGGGAGGTAGAGGTTCACTCATTGGTGCTATCGTAGGCGCAGGTGCAATCAATGCGATTAAGACTTATTTTACTGTCGCC
TACCCTGAGTTTTGGTTGCTTATCCTCGGTGGATTATTTGTGGTGGTTACTTTATTTCTCCCTAACGGCATCATTGGCGT
ACTTGATCGTTTTAAAAAGGAAAAAGAATAA

Upstream 100 bases:

>100_bases
GCTAAGATTGTATTACTGGTTCTTGTGATTTTGTTTATTCAAAAACGTCCACAAGGTTTATTCGCCCTCAAAGGCCGTTT
TGTGGATTAAGGAGAACCTC

Downstream 100 bases:

>100_bases
TGTTAATAACTAATGCTGATACTTCTAATGTTGCTACTTCCCAAAAAGTTACTAACGATGCGCTTGTTAAAAACAATCCT
AGTATCAATAATCCAATGAA

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MRLTQLLSDSPRNAILIGLCFLVLLILPWLHLLPETSSFHLSAYWITLIGKIMALAMVALALDLVWGYAGILSLGHGLYF
ALGGYAFGMYLTRETAGTGLPDFMRFLSWTELPWYWAGTQHFWWAMALVVLVPGLVAFIFGYFAFRSKIKGVYFSIITQA
MTYAAALLFFRNETGFGGNNGFTGFTTLLGYDITASSTRAVLCFTTALALLLSYLGLRYLMSQPYGRVLGAIRDSENRLQ
YLGYRTLWYKLSAWVLSAVIAGIAGALYVPQVGIINPGEMNPVNSIEMAVWVAAGGRGSLIGAIVGAGAINAIKTYFTVA
YPEFWLLILGGLFVVVTLFLPNGIIGVLDRFKKEKE

Sequences:

>Translated_356_residues
MRLTQLLSDSPRNAILIGLCFLVLLILPWLHLLPETSSFHLSAYWITLIGKIMALAMVALALDLVWGYAGILSLGHGLYF
ALGGYAFGMYLTRETAGTGLPDFMRFLSWTELPWYWAGTQHFWWAMALVVLVPGLVAFIFGYFAFRSKIKGVYFSIITQA
MTYAAALLFFRNETGFGGNNGFTGFTTLLGYDITASSTRAVLCFTTALALLLSYLGLRYLMSQPYGRVLGAIRDSENRLQ
YLGYRTLWYKLSAWVLSAVIAGIAGALYVPQVGIINPGEMNPVNSIEMAVWVAAGGRGSLIGAIVGAGAINAIKTYFTVA
YPEFWLLILGGLFVVVTLFLPNGIIGVLDRFKKEKE
>Mature_356_residues
MRLTQLLSDSPRNAILIGLCFLVLLILPWLHLLPETSSFHLSAYWITLIGKIMALAMVALALDLVWGYAGILSLGHGLYF
ALGGYAFGMYLTRETAGTGLPDFMRFLSWTELPWYWAGTQHFWWAMALVVLVPGLVAFIFGYFAFRSKIKGVYFSIITQA
MTYAAALLFFRNETGFGGNNGFTGFTTLLGYDITASSTRAVLCFTTALALLLSYLGLRYLMSQPYGRVLGAIRDSENRLQ
YLGYRTLWYKLSAWVLSAVIAGIAGALYVPQVGIINPGEMNPVNSIEMAVWVAAGGRGSLIGAIVGAGAINAIKTYFTVA
YPEFWLLILGGLFVVVTLFLPNGIIGVLDRFKKEKE

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=311, Percent_Identity=24.7588424437299, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 39167; Mature: 39167

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLTQLLSDSPRNAILIGLCFLVLLILPWLHLLPETSSFHLSAYWITLIGKIMALAMVAL
CCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ALDLVWGYAGILSLGHGLYFALGGYAFGMYLTRETAGTGLPDFMRFLSWTELPWYWAGTQ
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHCCCCEEECCCH
HFWWAMALVVLVPGLVAFIFGYFAFRSKIKGVYFSIITQAMTYAAALLFFRNETGFGGNN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GFTGFTTLLGYDITASSTRAVLCFTTALALLLSYLGLRYLMSQPYGRVLGAIRDSENRLQ
CCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHH
YLGYRTLWYKLSAWVLSAVIAGIAGALYVPQVGIINPGEMNPVNSIEMAVWVAAGGRGSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHH
IGAIVGAGAINAIKTYFTVAYPEFWLLILGGLFVVVTLFLPNGIIGVLDRFKKEKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MRLTQLLSDSPRNAILIGLCFLVLLILPWLHLLPETSSFHLSAYWITLIGKIMALAMVAL
CCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ALDLVWGYAGILSLGHGLYFALGGYAFGMYLTRETAGTGLPDFMRFLSWTELPWYWAGTQ
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHCCCCEEECCCH
HFWWAMALVVLVPGLVAFIFGYFAFRSKIKGVYFSIITQAMTYAAALLFFRNETGFGGNN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GFTGFTTLLGYDITASSTRAVLCFTTALALLLSYLGLRYLMSQPYGRVLGAIRDSENRLQ
CCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHH
YLGYRTLWYKLSAWVLSAVIAGIAGALYVPQVGIINPGEMNPVNSIEMAVWVAAGGRGSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHH
IGAIVGAGAINAIKTYFTVAYPEFWLLILGGLFVVVTLFLPNGIIGVLDRFKKEKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]