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The map label for this gene is gcvH

Identifier: 93005635

GI number: 93005635

Start: 949663

End: 950043

Strand: Reverse

Name: gcvH

Synonym: Pcryo_0805

Alternate gene names: 93005635

Gene position: 950043-949663 (Counterclockwise)

Preceding gene: 93005636

Following gene: 93005634

Centisome position: 31.05

GC content: 43.31

Gene sequence:

>381_bases
ATGAGTAATATCCCAGCAGAACTAAAATACATCGCCAGCCACGAGTGGTTACGCTTAGAAGACGACGGTACTATCACGGT
TGGTATCACTGATCATGCTCAAGACCTATTGGGTGACGTAGTATTTGTTGAATTGCCAGATGTTGGTGACATCATCGCTG
TTGATGACGAAATCTCTGTTGTTGAATCAGTAAAAGCAGCTTCTGACGTTTACGCGCCTATTTCAGGTGAAGTGGTTGCT
ATCAATGAAGCCTTAGAAGATGATCCAGAAATCATCAACTCTGATCCATATGGTGAAGGTTGGTTCTTCAGAATGAAGCC
TGACAATATCGCTGACTATGAAGCACTGCTAACCGCTGATGAGTACGAAAACGAGCTGTAA

Upstream 100 bases:

>100_bases
TGTCCGTAATGGCAAGCAGCAGTTTGACTCGTAGGCAACAGAAAATTTATTATTAGCTTTCTTTTTCATTAATTAACCTC
TATCACCTAGGAGAGAAACC

Downstream 100 bases:

>100_bases
TATCAGTCTTACGCTCTCTTAAAGGCAGCATTTAACTTTAGTATTGCTGCTTTTAATATTTTTATTGATAAGACAGTCTT
TGATAAGATTGTCATTTATA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MSNIPAELKYIASHEWLRLEDDGTITVGITDHAQDLLGDVVFVELPDVGDIIAVDDEISVVESVKAASDVYAPISGEVVA
INEALEDDPEIINSDPYGEGWFFRMKPDNIADYEALLTADEYENEL

Sequences:

>Translated_126_residues
MSNIPAELKYIASHEWLRLEDDGTITVGITDHAQDLLGDVVFVELPDVGDIIAVDDEISVVESVKAASDVYAPISGEVVA
INEALEDDPEIINSDPYGEGWFFRMKPDNIADYEALLTADEYENEL
>Mature_125_residues
SNIPAELKYIASHEWLRLEDDGTITVGITDHAQDLLGDVVFVELPDVGDIIAVDDEISVVESVKAASDVYAPISGEVVAI
NEALEDDPEIINSDPYGEGWFFRMKPDNIADYEALLTADEYENEL

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=116, Percent_Identity=43.9655172413793, Blast_Score=109, Evalue=7e-25,
Organism=Homo sapiens, GI89057342, Length=116, Percent_Identity=43.1034482758621, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI1789271, Length=123, Percent_Identity=60.9756097560976, Blast_Score=164, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17507493, Length=113, Percent_Identity=48.6725663716814, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17551294, Length=113, Percent_Identity=46.9026548672566, Blast_Score=106, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319272, Length=128, Percent_Identity=41.40625, Blast_Score=112, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17865652, Length=122, Percent_Identity=43.4426229508197, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_PSYA2 (Q4FTK7)

Other databases:

- EMBL:   CP000082
- RefSeq:   YP_264085.1
- ProteinModelPortal:   Q4FTK7
- SMR:   Q4FTK7
- STRING:   Q4FTK7
- GeneID:   3514646
- GenomeReviews:   CP000082_GR
- KEGG:   par:Psyc_0798
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- PhylomeDB:   Q4FTK7
- ProtClustDB:   PRK01202
- BioCyc:   PARC259536:PSYC_0798-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13886; Mature: 13755

Theoretical pI: Translated: 3.50; Mature: 3.50

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIPAELKYIASHEWLRLEDDGTITVGITDHAQDLLGDVVFVELPDVGDIIAVDDEISV
CCCCCCCEEEECCCCEEEECCCCEEEEEECCHHHHHHCCEEEEECCCCCCEEEECCCHHH
VESVKAASDVYAPISGEVVAINEALEDDPEIINSDPYGEGWFFRMKPDNIADYEALLTAD
HHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHEEEECC
EYENEL
CCCCCC
>Mature Secondary Structure 
SNIPAELKYIASHEWLRLEDDGTITVGITDHAQDLLGDVVFVELPDVGDIIAVDDEISV
CCCCCCEEEECCCCEEEECCCCEEEEEECCHHHHHHCCEEEEECCCCCCEEEECCCHHH
VESVKAASDVYAPISGEVVAINEALEDDPEIINSDPYGEGWFFRMKPDNIADYEALLTAD
HHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHEEEECC
EYENEL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA