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The map label for this gene is rluB [H]

Identifier: 93005438

GI number: 93005438

Start: 723132

End: 724064

Strand: Reverse

Name: rluB [H]

Synonym: Pcryo_0608

Alternate gene names: 93005438

Gene position: 724064-723132 (Counterclockwise)

Preceding gene: 93005472

Following gene: 93005434

Centisome position: 23.66

GC content: 45.55

Gene sequence:

>933_bases
ATGAAAGATGAAAAATTACAGAAAGCACTTGCCCGTATGGGACTTGGTTCACGTCGTCAGATGGAAGAAGTTATTAAAGC
AGGTCGTGTGACTGTCAATAATGGTCCGGCAACCATTGGTGATCGTGTTGAACAAGGTGATGAGATTCGCGTTGATGGTC
GTCAGATTAAATATACTTCTGAAAATGAGAAACGTCGTCGCGTTTTAGCTTATTACAAACCTGAAGGCGAAGTATGCTCG
GCGAATGATCCAGAAGGTCGTCCTACCGTGTTTGAGCGTTTGCCAAAGCTGACTCATGATCGTTGGGTTATGGTAGGTCG
CTTGGATATCAACTCAACTGGTCTATTATTATTTACTAACGATGGTGAAATGGCGCATCGTTTGATGCATCCTTCTAGTG
AAGTGACGCGTGAATATGCCGTACGTGTACTGGGCGATGTTACTCCAGATATGGCACGAGCGATGACAACAGGTGTCATG
TTAGAAGATGGCATGGCTAAGTTCGAAGATATTAAGGAAGGCGGTGGTGAAGGCGTCAACAAGTGGCATCATGTGCAGCT
TAAAGAAGGTCGTAACCGTGAAGTTCGCCGTTTATTTGAATCGCAAGGTCTTAAAGTCAGCCGTCTATTACGTACGAGCT
ATGGTAATATTACATTGCCAAAAGAGCTGCGTACAGGGCGCTTCTTAGAGCTAGATAGAAAAGACATTAATACGTTAACA
GACCTAGTGAGCTTACGCCCGCGTTATGGTACAGGTCTTCATGGTGCTGCTAAAGAAAAGCAAGAGCGTATCAGAAACAA
GCCGCTTAAAGCACGTCGTACTGACACTCGTGGCGATAATCGTAATAGCACTAGTAGTGCCAAACCGAAAAGCAGTGCAA
GTCCTGTCAGTCGCGGCACCCGTAATACCCGTGACCGCAACGATAAACGCTAA

Upstream 100 bases:

>100_bases
TATTTAAACATAATATCTATAACCCTTAGAAATAGCGTATAATATGCAGTCCAAATTTATACATTGAGAAATGTAAACCC
ACCGATTCTAGGAAATCGCT

Downstream 100 bases:

>100_bases
TATTTTTTAGATTTAGCATACTCAATAAAAAGCTGCCAATGATACATAATCATTGGCAGCTTTTTTGTATGTGACATTAA
TAAGTATTCTCTAGCCACTG

Product: pseudouridine synthase Rsu

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MKDEKLQKALARMGLGSRRQMEEVIKAGRVTVNNGPATIGDRVEQGDEIRVDGRQIKYTSENEKRRRVLAYYKPEGEVCS
ANDPEGRPTVFERLPKLTHDRWVMVGRLDINSTGLLLFTNDGEMAHRLMHPSSEVTREYAVRVLGDVTPDMARAMTTGVM
LEDGMAKFEDIKEGGGEGVNKWHHVQLKEGRNREVRRLFESQGLKVSRLLRTSYGNITLPKELRTGRFLELDRKDINTLT
DLVSLRPRYGTGLHGAAKEKQERIRNKPLKARRTDTRGDNRNSTSSAKPKSSASPVSRGTRNTRDRNDKR

Sequences:

>Translated_310_residues
MKDEKLQKALARMGLGSRRQMEEVIKAGRVTVNNGPATIGDRVEQGDEIRVDGRQIKYTSENEKRRRVLAYYKPEGEVCS
ANDPEGRPTVFERLPKLTHDRWVMVGRLDINSTGLLLFTNDGEMAHRLMHPSSEVTREYAVRVLGDVTPDMARAMTTGVM
LEDGMAKFEDIKEGGGEGVNKWHHVQLKEGRNREVRRLFESQGLKVSRLLRTSYGNITLPKELRTGRFLELDRKDINTLT
DLVSLRPRYGTGLHGAAKEKQERIRNKPLKARRTDTRGDNRNSTSSAKPKSSASPVSRGTRNTRDRNDKR
>Mature_310_residues
MKDEKLQKALARMGLGSRRQMEEVIKAGRVTVNNGPATIGDRVEQGDEIRVDGRQIKYTSENEKRRRVLAYYKPEGEVCS
ANDPEGRPTVFERLPKLTHDRWVMVGRLDINSTGLLLFTNDGEMAHRLMHPSSEVTREYAVRVLGDVTPDMARAMTTGVM
LEDGMAKFEDIKEGGGEGVNKWHHVQLKEGRNREVRRLFESQGLKVSRLLRTSYGNITLPKELRTGRFLELDRKDINTLT
DLVSLRPRYGTGLHGAAKEKQERIRNKPLKARRTDTRGDNRNSTSSAKPKSSASPVSRGTRNTRDRNDKR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=246, Percent_Identity=60.1626016260163, Blast_Score=290, Evalue=6e-80,
Organism=Escherichia coli, GI1790453, Length=216, Percent_Identity=30.0925925925926, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1788510, Length=176, Percent_Identity=32.9545454545455, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87081838, Length=190, Percent_Identity=30.5263157894737, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 35195; Mature: 35195

Theoretical pI: Translated: 10.75; Mature: 10.75

Prosite motif: PS50889 S4 ; PS00070 ALDEHYDE_DEHYDR_CYS ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDEKLQKALARMGLGSRRQMEEVIKAGRVTVNNGPATIGDRVEQGDEIRVDGRQIKYTS
CCHHHHHHHHHHCCCCCHHHHHHHHHHCCEEECCCCCCHHHHHCCCCEEEECCEEEEECC
ENEKRRRVLAYYKPEGEVCSANDPEGRPTVFERLPKLTHDRWVMVGRLDINSTGLLLFTN
CCCCCCEEEEEECCCCCEECCCCCCCCCHHHHHHHHCCCCCEEEEEEEEECCCEEEEEEC
DGEMAHRLMHPSSEVTREYAVRVLGDVTPDMARAMTTGVMLEDGMAKFEDIKEGGGEGVN
CHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KWHHVQLKEGRNREVRRLFESQGLKVSRLLRTSYGNITLPKELRTGRFLELDRKDINTLT
CEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHCCCCEECCHHHCCCCEEEECHHHHHHHH
DLVSLRPRYGTGLHGAAKEKQERIRNKPLKARRTDTRGDNRNSTSSAKPKSSASPVSRGT
HHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCC
RNTRDRNDKR
CCCCCCCCCC
>Mature Secondary Structure
MKDEKLQKALARMGLGSRRQMEEVIKAGRVTVNNGPATIGDRVEQGDEIRVDGRQIKYTS
CCHHHHHHHHHHCCCCCHHHHHHHHHHCCEEECCCCCCHHHHHCCCCEEEECCEEEEECC
ENEKRRRVLAYYKPEGEVCSANDPEGRPTVFERLPKLTHDRWVMVGRLDINSTGLLLFTN
CCCCCCEEEEEECCCCCEECCCCCCCCCHHHHHHHHCCCCCEEEEEEEEECCCEEEEEEC
DGEMAHRLMHPSSEVTREYAVRVLGDVTPDMARAMTTGVMLEDGMAKFEDIKEGGGEGVN
CHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KWHHVQLKEGRNREVRRLFESQGLKVSRLLRTSYGNITLPKELRTGRFLELDRKDINTLT
CEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHCCCCEECCHHHCCCCEEEECHHHHHHHH
DLVSLRPRYGTGLHGAAKEKQERIRNKPLKARRTDTRGDNRNSTSSAKPKSSASPVSRGT
HHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCC
RNTRDRNDKR
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]