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The map label for this gene is yfjQ [H]

Identifier: 93005472

GI number: 93005472

Start: 759660

End: 760643

Strand: Reverse

Name: yfjQ [H]

Synonym: Pcryo_0642

Alternate gene names: 93005472

Gene position: 760643-759660 (Counterclockwise)

Preceding gene: 93005473

Following gene: 93005438

Centisome position: 24.86

GC content: 41.87

Gene sequence:

>984_bases
GTGGTCAATTGTTATGCTTATTCCCGTAAAACGGGTGAACAGCTGAACAAAATAGCGCTCAGGGATGTCAGTCGCGCCTT
GAGCAATAGTGGTCAGTTTATCTGGTTAGGTCTTTATGAGCCTAGCCTGCAGACAATATCCGAGGTAAAAGAAGCCTTTG
ATCTGCACGAGCTTTCAATCGAAGATGCCTTTGCTGACCATCAACGCGCCAAAGTTGAAAGTTATGATAACGATACGATA
TTTGTGGTATTACGTACCGCTAAGCTTGAAGGCAATGTCATCCGCTACGGTACTACGGCGATATTTATGGGCAAGAACTA
TCTGATTACGATTCGTAATGGTCCTTCTAACTCCTATGCGCCGGTACGTGAGCACTGCAATCGACGCCCTGAAAAACTGC
GTATGGGACCTATATTTGTCTTGCACGCTGTAATTGATTTTATCGTCGATAACTATATGCCAGTGACCGACCGCTTAGGC
AGCTATTTACGTGAGCAAGAGCGCAATATTTTTACTTATGAATTTAATAAAGAAACGCTTAGAAATCTATATGAATTAAA
ATCACAATTGGTGCATATGCGTGCAGTTATTTTACCAGTGCAGGATATCTGTAGCTTTTTTATAAACCATCAAAAAAGCG
ATTTGGTCTCACCATTTTCGCACGCTGCAAAGCCCTACTTTCGAGATGTGAATGACCATTTACTGCATTCTTTAGATGCC
ATCAATGGTTTAAATGAGATGCTCAGTGTGGTGATGAATACATATCTCGCTATGGTAAATATGGGACAGAATGAGGTGGT
TCGCAAGCTTGCGGCTTGGGCAGGTATTTTGGCAGTACCGACGGCGATTGCTGGTATTTATGGCATGAACTTCGACTTTA
TGCCTGAGCTACACTGGCAATATTCTTACTTTATCATTATGGCGGTTATCATTTCACTATGTAGCTTTTTATACCTTAAC
TTTAAGCGCTTGGGCTGGTTATAA

Upstream 100 bases:

>100_bases
CGATTCTGATGCGACGGTCGAGGACACTATTGAGACCATGACCGTCTATGATCCAGATTCTGAGCGCTATGAGGATATTG
AGCCACTCAGTGCTAATGAA

Downstream 100 bases:

>100_bases
ACCCGCTTTTTAGATAAAAAAAGAGCTTCCATATATATAGAAGCTCTTTTTTTATTAACAGATATTAACTATCGTTAGTA
ACTCATCGACACCTATCATC

Product: magnesium and cobalt transport protein CorA

Products: Ni2; [Cytoplasm]; Co; 2 [Cytoplasm]; Mg (II) [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MVNCYAYSRKTGEQLNKIALRDVSRALSNSGQFIWLGLYEPSLQTISEVKEAFDLHELSIEDAFADHQRAKVESYDNDTI
FVVLRTAKLEGNVIRYGTTAIFMGKNYLITIRNGPSNSYAPVREHCNRRPEKLRMGPIFVLHAVIDFIVDNYMPVTDRLG
SYLREQERNIFTYEFNKETLRNLYELKSQLVHMRAVILPVQDICSFFINHQKSDLVSPFSHAAKPYFRDVNDHLLHSLDA
INGLNEMLSVVMNTYLAMVNMGQNEVVRKLAAWAGILAVPTAIAGIYGMNFDFMPELHWQYSYFIIMAVIISLCSFLYLN
FKRLGWL

Sequences:

>Translated_327_residues
MVNCYAYSRKTGEQLNKIALRDVSRALSNSGQFIWLGLYEPSLQTISEVKEAFDLHELSIEDAFADHQRAKVESYDNDTI
FVVLRTAKLEGNVIRYGTTAIFMGKNYLITIRNGPSNSYAPVREHCNRRPEKLRMGPIFVLHAVIDFIVDNYMPVTDRLG
SYLREQERNIFTYEFNKETLRNLYELKSQLVHMRAVILPVQDICSFFINHQKSDLVSPFSHAAKPYFRDVNDHLLHSLDA
INGLNEMLSVVMNTYLAMVNMGQNEVVRKLAAWAGILAVPTAIAGIYGMNFDFMPELHWQYSYFIIMAVIISLCSFLYLN
FKRLGWL
>Mature_327_residues
MVNCYAYSRKTGEQLNKIALRDVSRALSNSGQFIWLGLYEPSLQTISEVKEAFDLHELSIEDAFADHQRAKVESYDNDTI
FVVLRTAKLEGNVIRYGTTAIFMGKNYLITIRNGPSNSYAPVREHCNRRPEKLRMGPIFVLHAVIDFIVDNYMPVTDRLG
SYLREQERNIFTYEFNKETLRNLYELKSQLVHMRAVILPVQDICSFFINHQKSDLVSPFSHAAKPYFRDVNDHLLHSLDA
INGLNEMLSVVMNTYLAMVNMGQNEVVRKLAAWAGILAVPTAIAGIYGMNFDFMPELHWQYSYFIIMAVIISLCSFLYLN
FKRLGWL

Specific function: Plays A Role In The Transport Of Magnesium And Cobalt Ions. [C]

COG id: COG0598

COG function: function code P; Mg2+ and Co2+ transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family [H]

Homologues:

Organism=Escherichia coli, GI2367297, Length=152, Percent_Identity=29.6052631578947, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004488
- InterPro:   IPR002523 [H]

Pfam domain/function: PF01544 CorA [H]

EC number: NA

Molecular weight: Translated: 37677; Mature: 37677

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNCYAYSRKTGEQLNKIALRDVSRALSNSGQFIWLGLYEPSLQTISEVKEAFDLHELSI
CCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
EDAFADHQRAKVESYDNDTIFVVLRTAKLEGNVIRYGTTAIFMGKNYLITIRNGPSNSYA
HHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCEEEECCEEEEECCCEEEEEECCCCCCCC
PVREHCNRRPEKLRMGPIFVLHAVIDFIVDNYMPVTDRLGSYLREQERNIFTYEFNKETL
HHHHHCCCCCHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEECCHHHH
RNLYELKSQLVHMRAVILPVQDICSFFINHQKSDLVSPFSHAAKPYFRDVNDHLLHSLDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHCCHHHHCHHHHHHHHHHH
INGLNEMLSVVMNTYLAMVNMGQNEVVRKLAAWAGILAVPTAIAGIYGMNFDFMPELHWQ
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
YSYFIIMAVIISLCSFLYLNFKRLGWL
HHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVNCYAYSRKTGEQLNKIALRDVSRALSNSGQFIWLGLYEPSLQTISEVKEAFDLHELSI
CCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
EDAFADHQRAKVESYDNDTIFVVLRTAKLEGNVIRYGTTAIFMGKNYLITIRNGPSNSYA
HHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCEEEECCEEEEECCCEEEEEECCCCCCCC
PVREHCNRRPEKLRMGPIFVLHAVIDFIVDNYMPVTDRLGSYLREQERNIFTYEFNKETL
HHHHHCCCCCHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEECCHHHH
RNLYELKSQLVHMRAVILPVQDICSFFINHQKSDLVSPFSHAAKPYFRDVNDHLLHSLDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHCCHHHHCHHHHHHHHHHH
INGLNEMLSVVMNTYLAMVNMGQNEVVRKLAAWAGILAVPTAIAGIYGMNFDFMPELHWQ
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
YSYFIIMAVIISLCSFLYLNFKRLGWL
HHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; Co; 2 [Periplasm]; Mg (II) [Periplasm] [C]

Specific reaction: Ni2+ [Periplasm] = Ni2+ [Cytoplasm] Co+2 [Periplasm] = Co+2 [Cytoplasm] Mg (II) [Periplasm] = Mg (II) [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]