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The map label for this gene is holA [C]

Identifier: 93005394

GI number: 93005394

Start: 670186

End: 671262

Strand: Reverse

Name: holA [C]

Synonym: Pcryo_0564

Alternate gene names: 93005394

Gene position: 671262-670186 (Counterclockwise)

Preceding gene: 93005395

Following gene: 93005393

Centisome position: 21.94

GC content: 45.68

Gene sequence:

>1077_bases
ATGCAAGATACTTTTATACAAGCTTATCCAAAGCTACAGCGATCCTCAGTTGCTGTAGCAGGCTTGTGGCTAGCACATGG
TGATGAGCCATTATTAAGTCAATGGCTCATCGATGCGCTGCGTCCACATTGGCGCGCGCAGAATTACGCCATTAAACGTA
TTGAGCTTATATCGGTTAAGAGCTGGCAAGAAGTATTGTCAGAGCTTGGCAGCCAGTCTTTATTTGATGATGCCAGTGCC
TTGATTGTCACAGGCAACCATAAACCTGATAAGGCAGTCATTACAGAGCTTGAGCGCTTTGCACAGGACGCTCAAGCAGG
CAGCCACAGTCATAGCTTATTATGGTTGACCCCAAAACAAGACAAACGCGCTCAGAGCAGCAAGTGGTTTACGCCATTTG
CGCAATATGGTCACGTCATCGACTGTAATTTGTATAATGAGCAGCAGCGTCAGCAGTTATTGCAGATACAAGCGCAGCAG
TTTGGCTTAAAGCTATCGCAAGAAGCGTGGCAGCTACTCATGTCACATACTGAACATCATTTGCTCAGTGCCTATCAAAC
CTTATGGCGCTTATCTTATTTGTTTGCCCCACAACTCATGGCAAATAATGATGCTGCCATTCAAGAAACTACAAACTCTA
GCCCGCACCTATCACAGGTCGCACTAGATATTAATGACTTACAAGCGGCATTGGTCAGCGATGCGCAGTTTAGCGTGTTT
GATTTGTCCGATGCGATGCTTGCTGGCAATAGTGCCCAAGTCGCTAAAATCATTTTTCAACTCAAAGCCACGGATGAGCC
AACGACCTTAGTATTATGGGCAATCAGTAAAGACATGCGACAAATCATGCAATTGATGGATGGACAAGATCCGCAAGCCC
TAGGTATTTGGCGCAGTAAACAAGGCTTATATCAGCAAGCCTGTCGTCGTCAGGCAAAAGCGCAATCAGCTGCGTGGCCT
GCTCTGCTTTATCGCTGTGATCAGGCAATTAAAGGGCTTATTATCCAGCCAGCATGGGAGCTATTACTACAAGCAGCGTT
AGAATTATCAGGAAAACGTTTATTCCCTACTAAATAA

Upstream 100 bases:

>100_bases
ACGGCGATTCTTGTACCCAAACCTTAATATGGCTCAAATGTAGCTTAGCAATTTTTTGGCTTTGCTTTTTTATTCACAAC
TTTGTGTGCGTCAGCCTTTT

Downstream 100 bases:

>100_bases
CCAAAGTGATGAATTCTTAGTTATTTATCAGTCAGCAACCAATAATACCTCTAAGAACGATATTATTTCTTAACCTGCTT
TTTCAACGATAAACTTCCTG

Product: DNA polymerase III subunit delta

Products: diphosphate; DNAn+1

Alternate protein names: DNA Polymerase III Delta Subunit; DNA Polymerase III; DNA Polymerase III Delta Subunit Probably ATP Hydrolase

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MQDTFIQAYPKLQRSSVAVAGLWLAHGDEPLLSQWLIDALRPHWRAQNYAIKRIELISVKSWQEVLSELGSQSLFDDASA
LIVTGNHKPDKAVITELERFAQDAQAGSHSHSLLWLTPKQDKRAQSSKWFTPFAQYGHVIDCNLYNEQQRQQLLQIQAQQ
FGLKLSQEAWQLLMSHTEHHLLSAYQTLWRLSYLFAPQLMANNDAAIQETTNSSPHLSQVALDINDLQAALVSDAQFSVF
DLSDAMLAGNSAQVAKIIFQLKATDEPTTLVLWAISKDMRQIMQLMDGQDPQALGIWRSKQGLYQQACRRQAKAQSAAWP
ALLYRCDQAIKGLIIQPAWELLLQAALELSGKRLFPTK

Sequences:

>Translated_358_residues
MQDTFIQAYPKLQRSSVAVAGLWLAHGDEPLLSQWLIDALRPHWRAQNYAIKRIELISVKSWQEVLSELGSQSLFDDASA
LIVTGNHKPDKAVITELERFAQDAQAGSHSHSLLWLTPKQDKRAQSSKWFTPFAQYGHVIDCNLYNEQQRQQLLQIQAQQ
FGLKLSQEAWQLLMSHTEHHLLSAYQTLWRLSYLFAPQLMANNDAAIQETTNSSPHLSQVALDINDLQAALVSDAQFSVF
DLSDAMLAGNSAQVAKIIFQLKATDEPTTLVLWAISKDMRQIMQLMDGQDPQALGIWRSKQGLYQQACRRQAKAQSAAWP
ALLYRCDQAIKGLIIQPAWELLLQAALELSGKRLFPTK
>Mature_358_residues
MQDTFIQAYPKLQRSSVAVAGLWLAHGDEPLLSQWLIDALRPHWRAQNYAIKRIELISVKSWQEVLSELGSQSLFDDASA
LIVTGNHKPDKAVITELERFAQDAQAGSHSHSLLWLTPKQDKRAQSSKWFTPFAQYGHVIDCNLYNEQQRQQLLQIQAQQ
FGLKLSQEAWQLLMSHTEHHLLSAYQTLWRLSYLFAPQLMANNDAAIQETTNSSPHLSQVALDINDLQAALVSDAQFSVF
DLSDAMLAGNSAQVAKIIFQLKATDEPTTLVLWAISKDMRQIMQLMDGQDPQALGIWRSKQGLYQQACRRQAKAQSAAWP
ALLYRCDQAIKGLIIQPAWELLLQAALELSGKRLFPTK

Specific function: DNA Polymerase III Is A Complex, Multichain Enzyme Responsible For Most Of The Replicative Synthesis In Bacteria. This DNA Polymerase Also Exhibits 3' To 5' Exonuclease Activity. The Delta Subunit Seems To Interact With The Gamma Subunit To Transfer The B

COG id: COG1466

COG function: function code L; DNA polymerase III, delta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.7

Molecular weight: Translated: 40429; Mature: 40429

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDTFIQAYPKLQRSSVAVAGLWLAHGDEPLLSQWLIDALRPHWRAQNYAIKRIELISVK
CCCCHHHHHHHHHHCCCHHEEEEECCCCCHHHHHHHHHHHCHHHCCCCHHHHHEEEEEHH
SWQEVLSELGSQSLFDDASALIVTGNHKPDKAVITELERFAQDAQAGSHSHSLLWLTPKQ
HHHHHHHHHCCCHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
DKRAQSSKWFTPFAQYGHVIDCNLYNEQQRQQLLQIQAQQFGLKLSQEAWQLLMSHTEHH
HHHHCCCCCCCHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LLSAYQTLWRLSYLFAPQLMANNDAAIQETTNSSPHLSQVALDINDLQAALVSDAQFSVF
HHHHHHHHHHHHHHHCCHHHCCCCCCEEECCCCCCCHHHHEECHHHHHHHHHCCCCEEEE
DLSDAMLAGNSAQVAKIIFQLKATDEPTTLVLWAISKDMRQIMQLMDGQDPQALGIWRSK
ECCHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCEEECHHCC
QGLYQQACRRQAKAQSAAWPALLYRCDQAIKGLIIQPAWELLLQAALELSGKRLFPTK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQDTFIQAYPKLQRSSVAVAGLWLAHGDEPLLSQWLIDALRPHWRAQNYAIKRIELISVK
CCCCHHHHHHHHHHCCCHHEEEEECCCCCHHHHHHHHHHHCHHHCCCCHHHHHEEEEEHH
SWQEVLSELGSQSLFDDASALIVTGNHKPDKAVITELERFAQDAQAGSHSHSLLWLTPKQ
HHHHHHHHHCCCHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
DKRAQSSKWFTPFAQYGHVIDCNLYNEQQRQQLLQIQAQQFGLKLSQEAWQLLMSHTEHH
HHHHCCCCCCCHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LLSAYQTLWRLSYLFAPQLMANNDAAIQETTNSSPHLSQVALDINDLQAALVSDAQFSVF
HHHHHHHHHHHHHHHCCHHHCCCCCCEEECCCCCCCHHHHEECHHHHHHHHHCCCCEEEE
DLSDAMLAGNSAQVAKIIFQLKATDEPTTLVLWAISKDMRQIMQLMDGQDPQALGIWRSK
ECCHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCEEECHHCC
QGLYQQACRRQAKAQSAAWPALLYRCDQAIKGLIIQPAWELLLQAALELSGKRLFPTK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: deoxynucleoside triphosphate; DNAn

Specific reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10 -Phenanthroline; 2', 3'-Dideoxythymidine 5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA