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The map label for this gene is macA [H]

Identifier: 93005393

GI number: 93005393

Start: 668580

End: 669950

Strand: Reverse

Name: macA [H]

Synonym: Pcryo_0563

Alternate gene names: 93005393

Gene position: 669950-668580 (Counterclockwise)

Preceding gene: 93005394

Following gene: 93005392

Centisome position: 21.89

GC content: 45.88

Gene sequence:

>1371_bases
ATGCGCAAAATTGGCAAAAACTCTGCTATTAAATGGGGCATTATTGCCCTCGTCATCATCGCTTTAGGGGTTTTAGCCTA
CAAAACCTTAAAACCAAAAGAAGTAACACCCAACTATCTAACAGCGACTGCTGAGATTGGCGATATCGAAAATAACGTTA
TGGCATCGGGCAAGGTTAAGGCTTTAAATACCGTCGATGTTGGTGCGCAAGTATCTGGTGAAGTGACGCGACTATTCGTT
GAAGTCGGTGATGAAGTGCAAAAAGGCGATTTGATTGCGCAGATTGACCAAGTAACGCAGAAGAACAGTCTGAGCAATGA
GCAAGCAAGCCTTGAGCAAAGTGAAGCGGCGCTACAAAGTGCACGAGCTGAGTCGTTAAGTCGTCAAGCAGGTTTAAAAA
GTGCGCAGGCAGATCTTGCCAGCCGCCAAGCTGAGTTAAGACAAGCGCAAGCAGATTTTTCACGCTTGCAAGGCTTACTC
GCTATCGACGCGATATCACAGCAGGATTATGATACTCAAAGGACAAAGGTACAAACTGCGCAGGCAGCAGTCGCCAATGC
GCGTGCCTCTATCGATACTGCTAAAGCCGCTATTGCCACTACTGCAGCGAATATCAATAGTCAACAGGCGGCGTTACGTA
AATCTCAAACCAACGTCAGCACCGCGCAAGAGGATTTAAGCTACACCACCATTCGCGCGCCAATGTCAGGTACTGTAGTA
TCGGTAACGACTGAGCAAGGGACGACGGTCAATGCCAATCAAAGTGCACCGACCATTGTTACCTTAGCTGATTTATCTAC
CGTACGCATTAATGCTCAAATCTCTGAAGCTGACGTCATTAATGTCAAAGCGGGCTTGCCTGTTTATTTTAATATCATCG
GTAATCCCGATCAAAAATATGATTCGATTCTTAAAGCGATTGAACCGGCTCCTGAAAAAATCAGTGATACCAGCTCGACC
GATGCTGCCATTTATTATGTCGGTTATATTGAAGTACCCAATACCGAGCGCCGCTTTCGTATTGATATGACCGCGCAAGT
TTATATTGTCATTAACCAAGCCAAAGATGCGCTACTTATCCCTTCTGCCGCACTGCAACCTGCCGGTAAATCTAACAAAA
CTGGAAATAATAAAAAAGCATCTACCACTGCGGTTAATACTAATGGCGATTCTGCCGCAACTAAAGCTAGTGTCCGCGTT
CTTAAAACTGATGGCACAGTGGTTGAACAAGCTGTCACAGTTGGTATTAATAACCGCGTCAATGCGGAGATATTGAGTGG
TCTTAAAAAAGGTGACGAGGTTATCTTAAGCGAAGAGGGACCAGATAGTGGCAGCAAAGGCAGTGGTGGCAGAGGCGGCC
GACCATTTTAG

Upstream 100 bases:

>100_bases
ATATCTCTTCTTAACCTTTCGCAAACTAACTCCCATTCCCTTTTGTCCGTCGAGCCTCTATTATAGTTACATTTGATTTT
CTTGTTATTGGATTGTCGTC

Downstream 100 bases:

>100_bases
CCCTAACTCCAGAATCAGATAGAACAAGATTTAAATGAATTTAAATTAGCGACAAATCAGCTTTGATATTGATTAAGATA
AGCAGGATAGTAAATGAGCA

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MRKIGKNSAIKWGIIALVIIALGVLAYKTLKPKEVTPNYLTATAEIGDIENNVMASGKVKALNTVDVGAQVSGEVTRLFV
EVGDEVQKGDLIAQIDQVTQKNSLSNEQASLEQSEAALQSARAESLSRQAGLKSAQADLASRQAELRQAQADFSRLQGLL
AIDAISQQDYDTQRTKVQTAQAAVANARASIDTAKAAIATTAANINSQQAALRKSQTNVSTAQEDLSYTTIRAPMSGTVV
SVTTEQGTTVNANQSAPTIVTLADLSTVRINAQISEADVINVKAGLPVYFNIIGNPDQKYDSILKAIEPAPEKISDTSST
DAAIYYVGYIEVPNTERRFRIDMTAQVYIVINQAKDALLIPSAALQPAGKSNKTGNNKKASTTAVNTNGDSAATKASVRV
LKTDGTVVEQAVTVGINNRVNAEILSGLKKGDEVILSEEGPDSGSKGSGGRGGRPF

Sequences:

>Translated_456_residues
MRKIGKNSAIKWGIIALVIIALGVLAYKTLKPKEVTPNYLTATAEIGDIENNVMASGKVKALNTVDVGAQVSGEVTRLFV
EVGDEVQKGDLIAQIDQVTQKNSLSNEQASLEQSEAALQSARAESLSRQAGLKSAQADLASRQAELRQAQADFSRLQGLL
AIDAISQQDYDTQRTKVQTAQAAVANARASIDTAKAAIATTAANINSQQAALRKSQTNVSTAQEDLSYTTIRAPMSGTVV
SVTTEQGTTVNANQSAPTIVTLADLSTVRINAQISEADVINVKAGLPVYFNIIGNPDQKYDSILKAIEPAPEKISDTSST
DAAIYYVGYIEVPNTERRFRIDMTAQVYIVINQAKDALLIPSAALQPAGKSNKTGNNKKASTTAVNTNGDSAATKASVRV
LKTDGTVVEQAVTVGINNRVNAEILSGLKKGDEVILSEEGPDSGSKGSGGRGGRPF
>Mature_456_residues
MRKIGKNSAIKWGIIALVIIALGVLAYKTLKPKEVTPNYLTATAEIGDIENNVMASGKVKALNTVDVGAQVSGEVTRLFV
EVGDEVQKGDLIAQIDQVTQKNSLSNEQASLEQSEAALQSARAESLSRQAGLKSAQADLASRQAELRQAQADFSRLQGLL
AIDAISQQDYDTQRTKVQTAQAAVANARASIDTAKAAIATTAANINSQQAALRKSQTNVSTAQEDLSYTTIRAPMSGTVV
SVTTEQGTTVNANQSAPTIVTLADLSTVRINAQISEADVINVKAGLPVYFNIIGNPDQKYDSILKAIEPAPEKISDTSST
DAAIYYVGYIEVPNTERRFRIDMTAQVYIVINQAKDALLIPSAALQPAGKSNKTGNNKKASTTAVNTNGDSAATKASVRV
LKTDGTVVEQAVTVGINNRVNAEILSGLKKGDEVILSEEGPDSGSKGSGGRGGRPF

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=428, Percent_Identity=35.2803738317757, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1790519, Length=245, Percent_Identity=25.7142857142857, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787013, Length=314, Percent_Identity=24.5222929936306, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1789900, Length=259, Percent_Identity=26.6409266409266, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789041, Length=242, Percent_Identity=25.6198347107438, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 48062; Mature: 48062

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIGKNSAIKWGIIALVIIALGVLAYKTLKPKEVTPNYLTATAEIGDIENNVMASGKVK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCEECCCCEE
ALNTVDVGAQVSGEVTRLFVEVGDEVQKGDLIAQIDQVTQKNSLSNEQASLEQSEAALQS
EEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
ARAESLSRQAGLKSAQADLASRQAELRQAQADFSRLQGLLAIDAISQQDYDTQRTKVQTA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHCCCCCCHHHHHHHHH
QAAVANARASIDTAKAAIATTAANINSQQAALRKSQTNVSTAQEDLSYTTIRAPMSGTVV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCEEEEECCCCCCEE
SVTTEQGTTVNANQSAPTIVTLADLSTVRINAQISEADVINVKAGLPVYFNIIGNPDQKY
EEEECCCCEEECCCCCCEEEEEECCEEEEEEEEECCCEEEEEECCCCEEEEEECCCCHHH
DSILKAIEPAPEKISDTSSTDAAIYYVGYIEVPNTERRFRIDMTAQVYIVINQAKDALLI
HHHHHHHCCCCHHCCCCCCCCEEEEEEEEEECCCCCCEEEEEEEEEEEEEEECCCCEEEC
PSAALQPAGKSNKTGNNKKASTTAVNTNGDSAATKASVRVLKTDGTVVEQAVTVGINNRV
CHHHCCCCCCCCCCCCCCCCCEEEEECCCCCHHCEEEEEEEECCCCEEEHHEEECCCCCC
NAEILSGLKKGDEVILSEEGPDSGSKGSGGRGGRPF
CHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRKIGKNSAIKWGIIALVIIALGVLAYKTLKPKEVTPNYLTATAEIGDIENNVMASGKVK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCEECCCCEE
ALNTVDVGAQVSGEVTRLFVEVGDEVQKGDLIAQIDQVTQKNSLSNEQASLEQSEAALQS
EEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
ARAESLSRQAGLKSAQADLASRQAELRQAQADFSRLQGLLAIDAISQQDYDTQRTKVQTA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHCCCCCCHHHHHHHHH
QAAVANARASIDTAKAAIATTAANINSQQAALRKSQTNVSTAQEDLSYTTIRAPMSGTVV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCEEEEECCCCCCEE
SVTTEQGTTVNANQSAPTIVTLADLSTVRINAQISEADVINVKAGLPVYFNIIGNPDQKY
EEEECCCCEEECCCCCCEEEEEECCEEEEEEEEECCCEEEEEECCCCEEEEEECCCCHHH
DSILKAIEPAPEKISDTSSTDAAIYYVGYIEVPNTERRFRIDMTAQVYIVINQAKDALLI
HHHHHHHCCCCHHCCCCCCCCEEEEEEEEEECCCCCCEEEEEEEEEEEEEEECCCCEEEC
PSAALQPAGKSNKTGNNKKASTTAVNTNGDSAATKASVRVLKTDGTVVEQAVTVGINNRV
CHHHCCCCCCCCCCCCCCCCCEEEEECCCCCHHCEEEEEEEECCCCEEEHHEEECCCCCC
NAEILSGLKKGDEVILSEEGPDSGSKGSGGRGGRPF
CHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]