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The map label for this gene is gltJ [H]

Identifier: 93005166

GI number: 93005166

Start: 405914

End: 406660

Strand: Direct

Name: gltJ [H]

Synonym: Pcryo_0335

Alternate gene names: 93005166

Gene position: 405914-406660 (Clockwise)

Preceding gene: 93005165

Following gene: 93005167

Centisome position: 13.27

GC content: 44.98

Gene sequence:

>747_bases
ATGAATTATAGCTGGAACTGGGGTGTGCTGTTTGAGCAGACCGGTATCGGTAGCGAGCTGTATATCCATTGGATGATTAC
TGGTCTTGGCTGGTTGTTATTGATTGGCAGTATTGCATGGGCAATCGCCATGGTGGTTGGTACTATTTTTGGCATCATGC
GTACCTTGCCGAATAAAGGCGCACGTGCTATCGGTACTGCTTATGTGACGTTTTTCCGCAATATCCCTTTATTGGTACAA
TTATTTTTCTGGTTTTATATCGCCCCAGGTTGGCTCACGCCATCCATACAAGAGTGGTGGTATAAAGACTTGTCTCCCAA
TACCTCAGCCATGCTGTCAGCCAGTATCGGTCTTGGTTTATTTACCGCAGCTCGTATTGTTGAACAAGTACGTACTGGTA
TCGAGTCACTACCTCAAGGGCAGATCAATGCTGCCTACGCGCTTGGCTTTAGTATCCCGCAAGCCTATAAAGAAGTACTG
CTGCCACAAGCGTTTCGGATTATCTTACCGCCACTGAGCTCAGAGCTGACCAACTGCTTTAAAAACGCCTCAGTTGCGTC
TTTGGTTGGGGTAATGGAGCTGATCAGTCAGACCAAAACCATCAGTGAATATACTCAAAACAGTCTTGAGATTTATACCT
ATGCGACCATTATTTATCTGGTGTTTAATCTGTCTTTAATTGCCATTATGGGGCTAATTGAACGTAAATTGCGTGTGCCT
GGGCTAATTGCAGGGAGTCAAAAATGA

Upstream 100 bases:

>100_bases
TCCCTCAACATAGCATGCTTACGCGTTCAGATTGTTTGTCAAATAGTTTGATAGCAGTCTGAAAGCGTGGGCTTTTTAAC
CGTTGCTCGGAGAGACAATT

Downstream 100 bases:

>100_bases
ATATGACCGAACTTGCAACTGCCTATCCCGGTTTGATGGGTGGCATGCTGACGACTTTAAAAGTATTATTTTTAGCAATC
TTAGGCGGTCTCAGCTTAGG

Product: amino acid ABC transporter permease

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MNYSWNWGVLFEQTGIGSELYIHWMITGLGWLLLIGSIAWAIAMVVGTIFGIMRTLPNKGARAIGTAYVTFFRNIPLLVQ
LFFWFYIAPGWLTPSIQEWWYKDLSPNTSAMLSASIGLGLFTAARIVEQVRTGIESLPQGQINAAYALGFSIPQAYKEVL
LPQAFRIILPPLSSELTNCFKNASVASLVGVMELISQTKTISEYTQNSLEIYTYATIIYLVFNLSLIAIMGLIERKLRVP
GLIAGSQK

Sequences:

>Translated_248_residues
MNYSWNWGVLFEQTGIGSELYIHWMITGLGWLLLIGSIAWAIAMVVGTIFGIMRTLPNKGARAIGTAYVTFFRNIPLLVQ
LFFWFYIAPGWLTPSIQEWWYKDLSPNTSAMLSASIGLGLFTAARIVEQVRTGIESLPQGQINAAYALGFSIPQAYKEVL
LPQAFRIILPPLSSELTNCFKNASVASLVGVMELISQTKTISEYTQNSLEIYTYATIIYLVFNLSLIAIMGLIERKLRVP
GLIAGSQK
>Mature_248_residues
MNYSWNWGVLFEQTGIGSELYIHWMITGLGWLLLIGSIAWAIAMVVGTIFGIMRTLPNKGARAIGTAYVTFFRNIPLLVQ
LFFWFYIAPGWLTPSIQEWWYKDLSPNTSAMLSASIGLGLFTAARIVEQVRTGIESLPQGQINAAYALGFSIPQAYKEVL
LPQAFRIILPPLSSELTNCFKNASVASLVGVMELISQTKTISEYTQNSLEIYTYATIIYLVFNLSLIAIMGLIERKLRVP
GLIAGSQK

Specific function: Part of the binding-protein-dependent transport system for aspartate/glutamate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786874, Length=246, Percent_Identity=42.6829268292683, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI1786873, Length=214, Percent_Identity=30.8411214953271, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788226, Length=216, Percent_Identity=31.0185185185185, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI48994930, Length=226, Percent_Identity=29.2035398230088, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787086, Length=219, Percent_Identity=32.4200913242009, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082239, Length=213, Percent_Identity=26.2910798122066, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI1787030, Length=217, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788646, Length=201, Percent_Identity=25.8706467661692, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787087, Length=171, Percent_Identity=32.1637426900585, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788645, Length=206, Percent_Identity=27.6699029126214, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 27586; Mature: 27586

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYSWNWGVLFEQTGIGSELYIHWMITGLGWLLLIGSIAWAIAMVVGTIFGIMRTLPNKG
CCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ARAIGTAYVTFFRNIPLLVQLFFWFYIAPGWLTPSIQEWWYKDLSPNTSAMLSASIGLGL
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHH
FTAARIVEQVRTGIESLPQGQINAAYALGFSIPQAYKEVLLPQAFRIILPPLSSELTNCF
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KNASVASLVGVMELISQTKTISEYTQNSLEIYTYATIIYLVFNLSLIAIMGLIERKLRVP
HCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GLIAGSQK
CCCCCCCC
>Mature Secondary Structure
MNYSWNWGVLFEQTGIGSELYIHWMITGLGWLLLIGSIAWAIAMVVGTIFGIMRTLPNKG
CCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ARAIGTAYVTFFRNIPLLVQLFFWFYIAPGWLTPSIQEWWYKDLSPNTSAMLSASIGLGL
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHH
FTAARIVEQVRTGIESLPQGQINAAYALGFSIPQAYKEVLLPQAFRIILPPLSSELTNCF
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KNASVASLVGVMELISQTKTISEYTQNSLEIYTYATIIYLVFNLSLIAIMGLIERKLRVP
HCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GLIAGSQK
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]