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The map label for this gene is gltK [H]

Identifier: 93005167

GI number: 93005167

Start: 406657

End: 407325

Strand: Direct

Name: gltK [H]

Synonym: Pcryo_0336

Alternate gene names: 93005167

Gene position: 406657-407325 (Clockwise)

Preceding gene: 93005166

Following gene: 93005168

Centisome position: 13.29

GC content: 43.5

Gene sequence:

>669_bases
ATGAATATGACCGAACTTGCAACTGCCTATCCCGGTTTGATGGGTGGCATGCTGACGACTTTAAAAGTATTATTTTTAGC
AATCTTAGGCGGTCTCAGCTTAGGGACGGTATTGGCTTTGATGCGTTTGTCTGGTATCAAAGCACTTGAGATACCTGCAA
AGCTATACGTCAATTATTTCCGTTCTGTGCCATTGCTGCTCGTACTTCTCTGGTTTTATTTTGCCGTACCGATGATTTAT
TTTTGGGTCGCTGGTAAATATCTGCAACTCGATACAGCTTTTACCTCGTGTGTAGTCGCCTTTATGATGTTTGAAGCGGC
ATATTTTTCAGAGGTGGTACGTGCGGGTATCCAATCTATCGGCAGTGGACAGGTTAACGCTGCCAAAGCGCTCGGTATGA
CCTATGGGCAAACGATGCGCTTGATTATTTTGCCGCAGGCGTTTCGCAAGATGCTACCGCTGATTTTGCAGCAATGTATT
ATTTTATTCCAAGATACGACATTGGTTTTTGCAATTGGCTTGACGGATTTTTTCCGCGCCGCTTATGTACGCGGTGAGCT
GATGGGTTTATTAACGCCCTATATATTAGGAGCAGGCGCTGTTTATTTTATTATCAGTTTGAGTGCCTCTATTGGCGTAC
AACAAGTACAAAAGCGTTTAAGATTCTAA

Upstream 100 bases:

>100_bases
CGACCATTATTTATCTGGTGTTTAATCTGTCTTTAATTGCCATTATGGGGCTAATTGAACGTAAATTGCGTGTGCCTGGG
CTAATTGCAGGGAGTCAAAA

Downstream 100 bases:

>100_bases
TGATCTTTAATAAATTCATATCGGAAGCTGTACGTTAAGAGCTATATTTAATAATGATGAAAATAATGTGGTTAGGAGCC
AGTGATGAATGAGCCAGTAA

Product: amino acid ABC transporter permease

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MNMTELATAYPGLMGGMLTTLKVLFLAILGGLSLGTVLALMRLSGIKALEIPAKLYVNYFRSVPLLLVLLWFYFAVPMIY
FWVAGKYLQLDTAFTSCVVAFMMFEAAYFSEVVRAGIQSIGSGQVNAAKALGMTYGQTMRLIILPQAFRKMLPLILQQCI
ILFQDTTLVFAIGLTDFFRAAYVRGELMGLLTPYILGAGAVYFIISLSASIGVQQVQKRLRF

Sequences:

>Translated_222_residues
MNMTELATAYPGLMGGMLTTLKVLFLAILGGLSLGTVLALMRLSGIKALEIPAKLYVNYFRSVPLLLVLLWFYFAVPMIY
FWVAGKYLQLDTAFTSCVVAFMMFEAAYFSEVVRAGIQSIGSGQVNAAKALGMTYGQTMRLIILPQAFRKMLPLILQQCI
ILFQDTTLVFAIGLTDFFRAAYVRGELMGLLTPYILGAGAVYFIISLSASIGVQQVQKRLRF
>Mature_222_residues
MNMTELATAYPGLMGGMLTTLKVLFLAILGGLSLGTVLALMRLSGIKALEIPAKLYVNYFRSVPLLLVLLWFYFAVPMIY
FWVAGKYLQLDTAFTSCVVAFMMFEAAYFSEVVRAGIQSIGSGQVNAAKALGMTYGQTMRLIILPQAFRKMLPLILQQCI
ILFQDTTLVFAIGLTDFFRAAYVRGELMGLLTPYILGAGAVYFIISLSASIGVQQVQKRLRF

Specific function: Part of the binding-protein-dependent transport system for aspartate/glutamate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786873, Length=218, Percent_Identity=51.8348623853211, Blast_Score=208, Evalue=3e-55,
Organism=Escherichia coli, GI87082239, Length=208, Percent_Identity=34.6153846153846, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1788226, Length=197, Percent_Identity=36.5482233502538, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787030, Length=223, Percent_Identity=31.390134529148, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI1786874, Length=175, Percent_Identity=30.8571428571429, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788645, Length=168, Percent_Identity=29.7619047619048, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI48994930, Length=178, Percent_Identity=27.5280898876405, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787087, Length=222, Percent_Identity=24.3243243243243, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 24520; Mature: 24520

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMTELATAYPGLMGGMLTTLKVLFLAILGGLSLGTVLALMRLSGIKALEIPAKLYVNYF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
RSVPLLLVLLWFYFAVPMIYFWVAGKYLQLDTAFTSCVVAFMMFEAAYFSEVVRAGIQSI
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSGQVNAAKALGMTYGQTMRLIILPQAFRKMLPLILQQCIILFQDTTLVFAIGLTDFFRA
CCCCCHHHHHHHHHHCCHHHHEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AYVRGELMGLLTPYILGAGAVYFIISLSASIGVQQVQKRLRF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure
MNMTELATAYPGLMGGMLTTLKVLFLAILGGLSLGTVLALMRLSGIKALEIPAKLYVNYF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
RSVPLLLVLLWFYFAVPMIYFWVAGKYLQLDTAFTSCVVAFMMFEAAYFSEVVRAGIQSI
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSGQVNAAKALGMTYGQTMRLIILPQAFRKMLPLILQQCIILFQDTTLVFAIGLTDFFRA
CCCCCHHHHHHHHHHCCHHHHEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AYVRGELMGLLTPYILGAGAVYFIISLSASIGVQQVQKRLRF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]