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The map label for this gene is dpnC [H]

Identifier: 93004984

GI number: 93004984

Start: 180938

End: 181597

Strand: Reverse

Name: dpnC [H]

Synonym: Pcryo_0153

Alternate gene names: 93004984

Gene position: 181597-180938 (Counterclockwise)

Preceding gene: 93004987

Following gene: 93004981

Centisome position: 5.93

GC content: 37.58

Gene sequence:

>660_bases
ATGAACCTAAATTTTAATCAAAGTCTTGCCAAAAAATATAAATCTGAACCTCAAAAAATTCGTGTGCTTAGCGAAGATTG
GGTAGCTAAGCAAAGTTATTGTCCTAATTGTAATACCGAACCATTGGCTGCATTTACGAACGGCAAACCTGTCGCTGACT
TTTATTGTGCAAACTGTGATGAGCAATACGAATTAAAAAGTAAGAAGGCTAAATTAAGTAACATTATTAATGATGGCGCT
TATGCCACCATGATTGAACGTATCAATAGCAAGGATAATCCCAGCTTTTTCTTTTTGACCTACTCTCAAGAATATAGGGT
TAATAACTTCTTAATCATTCCCAAACAATTCTTCAAGCCCGACATGATTATAAAACGCAAACCGTTATCCGTTACTGCCA
AACGTGCGGGCTGGGTTGGCTGTAATATTGACTTGCGCAAAGTGCCCGAATCAGGAAAAGTATTTTTAGTCAAAGACCAG
CAGGTCATACCACGAGATAATGTCACTGAGCAATTTCAAAAAACCTTATTTTTACGTACACAATCTATCGCCTCACGCGG
CTGGACACTAGATATACTGCAATGTATTGATAAGCTAGAGGCTAATTTCTCTCAATCAGGTTTATGCGTTTGCCGACTTA
TTAAAACTCAAATATCCTGA

Upstream 100 bases:

>100_bases
AGCTTATCCATTAACTTAGCGTTTAAGCTCATTTATATGGCTTAATACTCATATCGCTTATTTATCACTTCAAATCTTTC
CTTTAATAAGAAAACGGGCT

Downstream 100 bases:

>100_bases
AAACAACCACATTAAAGATAAAATCCGTCAGCAATTACAAATATTACGCGATAGAGGCATTATTAAGTTTTTGGGTCGTG
GTCATTATCAAAAATTATAT

Product: putative type II restriction endonuclease DpnI

Products: NA

Alternate protein names: R.DpnI; Endonuclease DpnI; Type II restriction enzyme DpnI [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MNLNFNQSLAKKYKSEPQKIRVLSEDWVAKQSYCPNCNTEPLAAFTNGKPVADFYCANCDEQYELKSKKAKLSNIINDGA
YATMIERINSKDNPSFFFLTYSQEYRVNNFLIIPKQFFKPDMIIKRKPLSVTAKRAGWVGCNIDLRKVPESGKVFLVKDQ
QVIPRDNVTEQFQKTLFLRTQSIASRGWTLDILQCIDKLEANFSQSGLCVCRLIKTQIS

Sequences:

>Translated_219_residues
MNLNFNQSLAKKYKSEPQKIRVLSEDWVAKQSYCPNCNTEPLAAFTNGKPVADFYCANCDEQYELKSKKAKLSNIINDGA
YATMIERINSKDNPSFFFLTYSQEYRVNNFLIIPKQFFKPDMIIKRKPLSVTAKRAGWVGCNIDLRKVPESGKVFLVKDQ
QVIPRDNVTEQFQKTLFLRTQSIASRGWTLDILQCIDKLEANFSQSGLCVCRLIKTQIS
>Mature_219_residues
MNLNFNQSLAKKYKSEPQKIRVLSEDWVAKQSYCPNCNTEPLAAFTNGKPVADFYCANCDEQYELKSKKAKLSNIINDGA
YATMIERINSKDNPSFFFLTYSQEYRVNNFLIIPKQFFKPDMIIKRKPLSVTAKRAGWVGCNIDLRKVPESGKVFLVKDQ
QVIPRDNVTEQFQKTLFLRTQSIASRGWTLDILQCIDKLEANFSQSGLCVCRLIKTQIS

Specific function: Recognizes the double-stranded and methylated sequence G(Me)ATC and cleaves after A-2 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010324 [H]

Pfam domain/function: PF06044 DRP [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 25145; Mature: 25145

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNFNQSLAKKYKSEPQKIRVLSEDWVAKQSYCPNCNTEPLAAFTNGKPVADFYCANCD
CCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCCCCEEEECCCCCCHHEEECCCC
EQYELKSKKAKLSNIINDGAYATMIERINSKDNPSFFFLTYSQEYRVNNFLIIPKQFFKP
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECHHHCCC
DMIIKRKPLSVTAKRAGWVGCNIDLRKVPESGKVFLVKDQQVIPRDNVTEQFQKTLFLRT
CCEEECCCCEEEECCCCEEECCCCCEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
QSIASRGWTLDILQCIDKLEANFSQSGLCVCRLIKTQIS
HHHHHCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNLNFNQSLAKKYKSEPQKIRVLSEDWVAKQSYCPNCNTEPLAAFTNGKPVADFYCANCD
CCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCCCCEEEECCCCCCHHEEECCCC
EQYELKSKKAKLSNIINDGAYATMIERINSKDNPSFFFLTYSQEYRVNNFLIIPKQFFKP
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECHHHCCC
DMIIKRKPLSVTAKRAGWVGCNIDLRKVPESGKVFLVKDQQVIPRDNVTEQFQKTLFLRT
CCEEECCCCEEEECCCCEEECCCCCEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
QSIASRGWTLDILQCIDKLEANFSQSGLCVCRLIKTQIS
HHHHHCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2844782; 3019562; 11463916 [H]