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The map label for this gene is aroE

Identifier: 93004981

GI number: 93004981

Start: 177821

End: 178687

Strand: Reverse

Name: aroE

Synonym: Pcryo_0150

Alternate gene names: 93004981

Gene position: 178687-177821 (Counterclockwise)

Preceding gene: 93004984

Following gene: 93004979

Centisome position: 5.84

GC content: 46.6

Gene sequence:

>867_bases
ATGACCCAGCATTTTATTGTTATTGGTAATCCAATTGCCCACAGCAAGTCTCCTGAAATCCATACGTTATTTGGTACGCA
AGCAGGGCTTGATATCTGTTATCAGCGTCAATATTGCCCTGATGACGCAGCAAGCTTTACAGCAGTGATTGAGGCATTCT
TTCATGGTGGCGGCGTCGGTGCCAATGTGACTGTACCCTTTAAACAGATCGCTTATGAGTGCTGTGCAGCGCGAGGTGGC
TTATCTGAGCATGCCAAAGTCGCTGGCGCGGTCAATACATTATTATTAAATAAAGCTCTGCTAGCAAAAGGTGTACCGCC
AGTAGAGGCGTTATTTGGTGACAATACTGATGGTCAAGGATTGGTCAATCATATGCAGCGTTTAGGCTGGCCATTGAATG
GTGCACGCGTGGCAATCATTGGGGCAGGCGGTGCAGCGCGCGGCGTGGTATTGCCGCTGATAGAGGCTGGTATTGAAACG
CTGAGTATCGCCAATCGTACAGTGAGTAAGGCAGAAATTTTAGTCGATGAACTTAGCACCGCAAGCGAAAAGATAAATCA
ACATACTATTCAGACCTATGCGACGGCTGATTTAAGTGGCGACTTTGATATCATTATCAATGCCACATCCATCGGACTAT
CAGGTGAAACCTTGCCACTAACCGATAAGCTAAACTGCCAATATGCTTACGATATGATGTATGGACGTGAGCTGCCATTT
TTACAACATTTTTCTATCCGTGGTGCTCAGACATCGGATGGTTACGGCATGCTAATCGGGCAAGCGGCGTTAAGTTTTGA
GCGTTGGACAGGACGCGCTATTGATGTTGCGCAAGCGACTACCGCATTAGAAAACGTATCAACATAA

Upstream 100 bases:

>100_bases
TCAGGTTTGTCAAACGGTGCGTTATCCCTTATGATAAGTACGCTTTGATACCCTTACTCTATCACCCTTACTTATGCCCT
GTACCGCCAGTGGAGCCTTT

Downstream 100 bases:

>100_bases
TAAACGGTGCACGAGTGCAGCTATCATCTATCTAAATGACAAAAACGATCTACGTTTTTATACTAGCCAAATCGTGTCAG
CAGCCATTTTTCTATACGGC

Product: shikimate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MTQHFIVIGNPIAHSKSPEIHTLFGTQAGLDICYQRQYCPDDAASFTAVIEAFFHGGGVGANVTVPFKQIAYECCAARGG
LSEHAKVAGAVNTLLLNKALLAKGVPPVEALFGDNTDGQGLVNHMQRLGWPLNGARVAIIGAGGAARGVVLPLIEAGIET
LSIANRTVSKAEILVDELSTASEKINQHTIQTYATADLSGDFDIIINATSIGLSGETLPLTDKLNCQYAYDMMYGRELPF
LQHFSIRGAQTSDGYGMLIGQAALSFERWTGRAIDVAQATTALENVST

Sequences:

>Translated_288_residues
MTQHFIVIGNPIAHSKSPEIHTLFGTQAGLDICYQRQYCPDDAASFTAVIEAFFHGGGVGANVTVPFKQIAYECCAARGG
LSEHAKVAGAVNTLLLNKALLAKGVPPVEALFGDNTDGQGLVNHMQRLGWPLNGARVAIIGAGGAARGVVLPLIEAGIET
LSIANRTVSKAEILVDELSTASEKINQHTIQTYATADLSGDFDIIINATSIGLSGETLPLTDKLNCQYAYDMMYGRELPF
LQHFSIRGAQTSDGYGMLIGQAALSFERWTGRAIDVAQATTALENVST
>Mature_287_residues
TQHFIVIGNPIAHSKSPEIHTLFGTQAGLDICYQRQYCPDDAASFTAVIEAFFHGGGVGANVTVPFKQIAYECCAARGGL
SEHAKVAGAVNTLLLNKALLAKGVPPVEALFGDNTDGQGLVNHMQRLGWPLNGARVAIIGAGGAARGVVLPLIEAGIETL
SIANRTVSKAEILVDELSTASEKINQHTIQTYATADLSGDFDIIINATSIGLSGETLPLTDKLNCQYAYDMMYGRELPFL
QHFSIRGAQTSDGYGMLIGQAALSFERWTGRAIDVAQATTALENVST

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1789675, Length=287, Percent_Identity=43.205574912892, Blast_Score=179, Evalue=1e-46,
Organism=Escherichia coli, GI1787983, Length=281, Percent_Identity=27.0462633451957, Blast_Score=88, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6320332, Length=293, Percent_Identity=27.9863481228669, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_PSYCK (Q1QEG9)

Other databases:

- EMBL:   CP000323
- RefSeq:   YP_579418.1
- ProteinModelPortal:   Q1QEG9
- SMR:   Q1QEG9
- STRING:   Q1QEG9
- GeneID:   4035509
- GenomeReviews:   CP000323_GR
- KEGG:   pcr:Pcryo_0150
- NMPDR:   fig|335284.3.peg.753
- eggNOG:   COG0169
- HOGENOM:   HBG553408
- OMA:   DQYVVFG
- PhylomeDB:   Q1QEG9
- ProtClustDB:   CLSK838830
- BioCyc:   PCRY335284:PCRYO_0150-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR011342
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00507

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 30539; Mature: 30408

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: ACT_SITE 68-68

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQHFIVIGNPIAHSKSPEIHTLFGTQAGLDICYQRQYCPDDAASFTAVIEAFFHGGGVG
CCCEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCC
ANVTVPFKQIAYECCAARGGLSEHAKVAGAVNTLLLNKALLAKGVPPVEALFGDNTDGQG
CEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCH
LVNHMQRLGWPLNGARVAIIGAGGAARGVVLPLIEAGIETLSIANRTVSKAEILVDELST
HHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
ASEKINQHTIQTYATADLSGDFDIIINATSIGLSGETLPLTDKLNCQYAYDMMYGRELPF
HHHHHHHHHHHEEEEECCCCCEEEEEEEEEECCCCCEECCCCCCCCEEEEHHHHCCCCCH
LQHFSIRGAQTSDGYGMLIGQAALSFERWTGRAIDVAQATTALENVST
HHHEEECCCCCCCCCEEEEHHHHHHHHHHCCCEEEHHHHHHHHHHCCC
>Mature Secondary Structure 
TQHFIVIGNPIAHSKSPEIHTLFGTQAGLDICYQRQYCPDDAASFTAVIEAFFHGGGVG
CCEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCC
ANVTVPFKQIAYECCAARGGLSEHAKVAGAVNTLLLNKALLAKGVPPVEALFGDNTDGQG
CEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCH
LVNHMQRLGWPLNGARVAIIGAGGAARGVVLPLIEAGIETLSIANRTVSKAEILVDELST
HHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
ASEKINQHTIQTYATADLSGDFDIIINATSIGLSGETLPLTDKLNCQYAYDMMYGRELPF
HHHHHHHHHHHEEEEECCCCCEEEEEEEEEECCCCCEECCCCCCCCEEEEHHHHCCCCCH
LQHFSIRGAQTSDGYGMLIGQAALSFERWTGRAIDVAQATTALENVST
HHHEEECCCCCCCCCEEEEHHHHHHHHHHCCCEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA