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The map label for this gene is yiaO [H]

Identifier: 92115092

GI number: 92115092

Start: 3314327

End: 3315295

Strand: Direct

Name: yiaO [H]

Synonym: Csal_2976

Alternate gene names: 92115092

Gene position: 3314327-3315295 (Clockwise)

Preceding gene: 92115091

Following gene: 92115093

Centisome position: 89.66

GC content: 61.3

Gene sequence:

>969_bases
ATGTTCAAACAGCTACTCGCCAGCGCCGTCATCGGCTCGAGCCTGCTTGCGGCCCCGTTCGCCACGGCGGCGGACTACAC
CCTCAAGTTCGGTCACCTGGCCAACGAGGAACATATCTGGAACAAGGCCGCCGAACGCTTCAAGGAGATCGTCGAGAAAC
GCTCGGACGGCCGGATCGAGGTACAGATCTTTCCCAACGAACAGTTGGGGAACGAAATGGACGTCATCAACAGCATTCAG
CTCGGCACCGCCGACATGACCATTACCGGTGAAAGCCTGCAGAACTGGGCCCCAAAGGCGGCGATGATGGCCGTGCCTTA
TGCCTTCAGCGATTCGGGGCAGATGCGCAAGGCGGTCGAGGGCGACATCGGTGAGCAGATCGAAGCGCAGATCGAGGAGA
AGACCGGCCTGGTCCCGATCGCCTGGTTCGAGCGCGGTCCGCGTGAGCTGACCTCCAACCGGCCGATCAAGGAACCCGCC
GATCTCGACGGCCTGCGGCTGCGCGTACCCAACGTGCCGCTTTTCGTCGATACCTGGCAGGCGCTCGGCGCCAAGCCCAC
GCCGATGGCCTTCAGCGAGGTATTCACATCACTTCAGCAGGGCACCATCGATGCCCAGGAGAATCCCCTGTCGCTGATCG
AAAGTGCCAGCTTCGACGAGGTGCAGGACTACGTGAACCTGACCGACCACGTGCGCAGCTGGATCTACGTGGTGATCGGC
AAGCAGAAGCTCGAATCGATGCCCGAGGACCTGCAGAAGGTCGTTCTCGATGCCGCCGACAAGATGCAGAGCTACGAGGA
AGAGCTGTTCGCTGAAGATCAGAAGCGACTGCGCAAGACGCTCGAGGAAGCCGGCATGGAATTCGTCGAGGTCGACCAGC
AAGCCTTCGCCGAAAAGGCCCGGCCCGCCGTCGAGGCCTCGTTGAACGAAGAACAACAGGCCCTCTACCAGGCCATTCAG
GACCTCTGA

Upstream 100 bases:

>100_bases
TGACGCATCGCGCGATGCGCCTCGCGCTCCACAACGCTGGATTGCCGGACACTTCAATGCTCGACAACAACAGCCATCAA
TCGAAGTCAGGAGCAACGTC

Downstream 100 bases:

>100_bases
ATCGAGGCGCTCCGGGTCGGCGGTACCCCGCCGCCCCGGAGACGTCGCTCATTCACCGCAGGCTTTCAAGCAAGGGATTC
CCATGCGTGAACCCGATTCA

Product: TRAP dicarboxylate transporter subunit DctP

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MFKQLLASAVIGSSLLAAPFATAADYTLKFGHLANEEHIWNKAAERFKEIVEKRSDGRIEVQIFPNEQLGNEMDVINSIQ
LGTADMTITGESLQNWAPKAAMMAVPYAFSDSGQMRKAVEGDIGEQIEAQIEEKTGLVPIAWFERGPRELTSNRPIKEPA
DLDGLRLRVPNVPLFVDTWQALGAKPTPMAFSEVFTSLQQGTIDAQENPLSLIESASFDEVQDYVNLTDHVRSWIYVVIG
KQKLESMPEDLQKVVLDAADKMQSYEEELFAEDQKRLRKTLEEAGMEFVEVDQQAFAEKARPAVEASLNEEQQALYQAIQ
DL

Sequences:

>Translated_322_residues
MFKQLLASAVIGSSLLAAPFATAADYTLKFGHLANEEHIWNKAAERFKEIVEKRSDGRIEVQIFPNEQLGNEMDVINSIQ
LGTADMTITGESLQNWAPKAAMMAVPYAFSDSGQMRKAVEGDIGEQIEAQIEEKTGLVPIAWFERGPRELTSNRPIKEPA
DLDGLRLRVPNVPLFVDTWQALGAKPTPMAFSEVFTSLQQGTIDAQENPLSLIESASFDEVQDYVNLTDHVRSWIYVVIG
KQKLESMPEDLQKVVLDAADKMQSYEEELFAEDQKRLRKTLEEAGMEFVEVDQQAFAEKARPAVEASLNEEQQALYQAIQ
DL
>Mature_322_residues
MFKQLLASAVIGSSLLAAPFATAADYTLKFGHLANEEHIWNKAAERFKEIVEKRSDGRIEVQIFPNEQLGNEMDVINSIQ
LGTADMTITGESLQNWAPKAAMMAVPYAFSDSGQMRKAVEGDIGEQIEAQIEEKTGLVPIAWFERGPRELTSNRPIKEPA
DLDGLRLRVPNVPLFVDTWQALGAKPTPMAFSEVFTSLQQGTIDAQENPLSLIESASFDEVQDYVNLTDHVRSWIYVVIG
KQKLESMPEDLQKVVLDAADKMQSYEEELFAEDQKRLRKTLEEAGMEFVEVDQQAFAEKARPAVEASLNEEQQALYQAIQ
DL

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=286, Percent_Identity=34.965034965035, Blast_Score=203, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 36040; Mature: 36040

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKQLLASAVIGSSLLAAPFATAADYTLKFGHLANEEHIWNKAAERFKEIVEKRSDGRIE
CHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEE
VQIFPNEQLGNEMDVINSIQLGTADMTITGESLQNWAPKAAMMAVPYAFSDSGQMRKAVE
EEECCCHHCCCHHHHHHHHEECCCCEEEECHHHHHCCCCHHHHEECCEECCCCHHHHHHC
GDIGEQIEAQIEEKTGLVPIAWFERGPRELTSNRPIKEPADLDGLRLRVPNVPLFVDTWQ
CCHHHHHHHHHHHHCCCEEEHHHCCCCHHHHCCCCCCCCCCCCCEEEECCCCCEEEECHH
ALGAKPTPMAFSEVFTSLQQGTIDAQENPLSLIESASFDEVQDYVNLTDHVRSWIYVVIG
HCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEC
KQKLESMPEDLQKVVLDAADKMQSYEEELFAEDQKRLRKTLEEAGMEFVEVDQQAFAEKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
RPAVEASLNEEQQALYQAIQDL
CHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MFKQLLASAVIGSSLLAAPFATAADYTLKFGHLANEEHIWNKAAERFKEIVEKRSDGRIE
CHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEE
VQIFPNEQLGNEMDVINSIQLGTADMTITGESLQNWAPKAAMMAVPYAFSDSGQMRKAVE
EEECCCHHCCCHHHHHHHHEECCCCEEEECHHHHHCCCCHHHHEECCEECCCCHHHHHHC
GDIGEQIEAQIEEKTGLVPIAWFERGPRELTSNRPIKEPADLDGLRLRVPNVPLFVDTWQ
CCHHHHHHHHHHHHCCCEEEHHHCCCCHHHHCCCCCCCCCCCCCEEEECCCCCEEEECHH
ALGAKPTPMAFSEVFTSLQQGTIDAQENPLSLIESASFDEVQDYVNLTDHVRSWIYVVIG
HCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEC
KQKLESMPEDLQKVVLDAADKMQSYEEELFAEDQKRLRKTLEEAGMEFVEVDQQAFAEKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
RPAVEASLNEEQQALYQAIQDL
CHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]