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The map label for this gene is rspA [H]

Identifier: 92115090

GI number: 92115090

Start: 3311962

End: 3313173

Strand: Direct

Name: rspA [H]

Synonym: Csal_2974

Alternate gene names: 92115090

Gene position: 3311962-3313173 (Clockwise)

Preceding gene: 92115089

Following gene: 92115091

Centisome position: 89.59

GC content: 66.58

Gene sequence:

>1212_bases
ATGAAGATTCGCGACGCCTACACCATCGTCACCTGCCCCGGCCGTAACTTCGTGACGCTCAAGATCGTCACCGAGTCAGG
CACCCACGGCATCGGCGATGCCACCCTGAACGGCCGCGAGATGGCGGTGGCCGCCTATCTCGACGAACACGTCGTGCCGG
CACTGATCGGCCGCGATGCCGGCCGCATCGAGGACACCTGGCAGTACCTCTATCGCGGTGCCTACTGGCGGCGCGGCCCG
GTGACCATGACCGCCATCGCCGCCGTCGACATGGCGCTGTGGGACATCAAGGCCAAGGCCGCGGGCATGCCGTTGTATCA
GCTCCTCGGCGGCAAGAGCCGCGAGCGGGTGATGACCTACGCCCACTGCACCGGGCAGACGATCGAGGATTGTCTCGGGG
AAGTCGCTCGCCACGTCGAGCTGGGCTACCGGGCGGTACGCGTCCAGTCCGGCGTGCCGGGCATCGAGACCACCTACGGT
GTCGCCAAGACGCCCGGCGAGCGCTACGAGCCGGCCGACTCGTCGCTGCCCGCCGAGCACGTATGGAGCACCGAGAAATA
CCTCAATCACGCACCCAAGCTGTTCGCCGCGGTGCGCGAGCGTTTCGGTGACGACCTGCACGTCCTGCACGACGTCCACC
ATCGCCTGACGCCGATCGAAGCGGCACGACTGGGCAAGGCGGTCGAGCCCTATCACCTGTTCTGGCTGGAAGACTGCGTG
CCGGCCGAAAACCAGGAGAGTCTGCGCCTGATCCGCGAGCACACCACCACGCCGCTGGCGATCGGCGAGGTGTTCAACTC
GATCCACGACTGCCGCGAGCTGATCCAGAACCAGTGGATCGACTATATCCGCATGCCGCTGACCCACGGCGGCGGCATCA
CCGCGATGCGTCGCGTCGCCGACCTGGCGTCGCTGTATCACGTACGCACCGGCTTCCACGGCCCCACCGACCTGTCGCCG
GTATGCCTGGGCGCGGCGATCCACTTCGATACCTGGGTGCCCAACTTCGGCATCCAGGAGCACATGCCGCACACCGACGA
GACCGACGCGGTGTTCCCCCACGACTATCGCTTCGAGGATGGCCACTTCCTGGCCGGCGAGAGCCCGGGACACGGCGTCG
ACATCGACGAGGAACTCGCTGCCAAGTATCCCTACGAACGCGCCAGCCTGCCGGTCAACCGTCTGGAAGACGGCACGCTC
TGGCACTGGTGA

Upstream 100 bases:

>100_bases
TCCTCAGCTCGCTGCCGGCCCTGGTCGAGCGCTTCCCCGACCTTTTCGAGGCCGCCGCGTCCAGCGGTCGTCGAACCCCG
CTGCCCTCTGGAGTCAGTGC

Downstream 100 bases:

>100_bases
TCCCGTCTTCCCTTATCCAGAGCGGAGTCCGTCATGAAAGCATTCGAGGTTCGCGCCCCCCACGACTTCGGCATCGTCGA
CATCGATGCCCCGCAGGCCG

Product: mandelate racemase/muconate lactonizing enzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MKIRDAYTIVTCPGRNFVTLKIVTESGTHGIGDATLNGREMAVAAYLDEHVVPALIGRDAGRIEDTWQYLYRGAYWRRGP
VTMTAIAAVDMALWDIKAKAAGMPLYQLLGGKSRERVMTYAHCTGQTIEDCLGEVARHVELGYRAVRVQSGVPGIETTYG
VAKTPGERYEPADSSLPAEHVWSTEKYLNHAPKLFAAVRERFGDDLHVLHDVHHRLTPIEAARLGKAVEPYHLFWLEDCV
PAENQESLRLIREHTTTPLAIGEVFNSIHDCRELIQNQWIDYIRMPLTHGGGITAMRRVADLASLYHVRTGFHGPTDLSP
VCLGAAIHFDTWVPNFGIQEHMPHTDETDAVFPHDYRFEDGHFLAGESPGHGVDIDEELAAKYPYERASLPVNRLEDGTL
WHW

Sequences:

>Translated_403_residues
MKIRDAYTIVTCPGRNFVTLKIVTESGTHGIGDATLNGREMAVAAYLDEHVVPALIGRDAGRIEDTWQYLYRGAYWRRGP
VTMTAIAAVDMALWDIKAKAAGMPLYQLLGGKSRERVMTYAHCTGQTIEDCLGEVARHVELGYRAVRVQSGVPGIETTYG
VAKTPGERYEPADSSLPAEHVWSTEKYLNHAPKLFAAVRERFGDDLHVLHDVHHRLTPIEAARLGKAVEPYHLFWLEDCV
PAENQESLRLIREHTTTPLAIGEVFNSIHDCRELIQNQWIDYIRMPLTHGGGITAMRRVADLASLYHVRTGFHGPTDLSP
VCLGAAIHFDTWVPNFGIQEHMPHTDETDAVFPHDYRFEDGHFLAGESPGHGVDIDEELAAKYPYERASLPVNRLEDGTL
WHW
>Mature_403_residues
MKIRDAYTIVTCPGRNFVTLKIVTESGTHGIGDATLNGREMAVAAYLDEHVVPALIGRDAGRIEDTWQYLYRGAYWRRGP
VTMTAIAAVDMALWDIKAKAAGMPLYQLLGGKSRERVMTYAHCTGQTIEDCLGEVARHVELGYRAVRVQSGVPGIETTYG
VAKTPGERYEPADSSLPAEHVWSTEKYLNHAPKLFAAVRERFGDDLHVLHDVHHRLTPIEAARLGKAVEPYHLFWLEDCV
PAENQESLRLIREHTTTPLAIGEVFNSIHDCRELIQNQWIDYIRMPLTHGGGITAMRRVADLASLYHVRTGFHGPTDLSP
VCLGAAIHFDTWVPNFGIQEHMPHTDETDAVFPHDYRFEDGHFLAGESPGHGVDIDEELAAKYPYERASLPVNRLEDGTL
WHW

Specific function: Probably involved in the degradation of homoserine lactone (HSL) or of a metabolite of HSL that signals starvation [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787864, Length=404, Percent_Identity=66.3366336633663, Blast_Score=580, Evalue=1e-167,
Organism=Escherichia coli, GI48994953, Length=414, Percent_Identity=30.4347826086957, Blast_Score=167, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 45208; Mature: 45208

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRDAYTIVTCPGRNFVTLKIVTESGTHGIGDATLNGREMAVAAYLDEHVVPALIGRDA
CCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCCEECCCCEEHHHHHHHHHHHHHHCCCC
GRIEDTWQYLYRGAYWRRGPVTMTAIAAVDMALWDIKAKAAGMPLYQLLGGKSRERVMTY
CCHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHEEEE
AHCTGQTIEDCLGEVARHVELGYRAVRVQSGVPGIETTYGVAKTPGERYEPADSSLPAEH
HHCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
VWSTEKYLNHAPKLFAAVRERFGDDLHVLHDVHHRLTPIEAARLGKAVEPYHLFWLEDCV
HHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEEECCCC
PAENQESLRLIREHTTTPLAIGEVFNSIHDCRELIQNQWIDYIRMPLTHGGGITAMRRVA
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
DLASLYHVRTGFHGPTDLSPVCLGAAIHFDTWVPNFGIQEHMPHTDETDAVFPHDYRFED
HHHHHHHHHCCCCCCCCCCHHHHHHEEEHHCCCCCCCCHHCCCCCCCCCCCCCCCCEECC
GHFLAGESPGHGVDIDEELAAKYPYERASLPVNRLEDGTLWHW
CCEECCCCCCCCCCCCHHHHHHCCCHHHCCCHHHCCCCCCCCC
>Mature Secondary Structure
MKIRDAYTIVTCPGRNFVTLKIVTESGTHGIGDATLNGREMAVAAYLDEHVVPALIGRDA
CCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCCEECCCCEEHHHHHHHHHHHHHHCCCC
GRIEDTWQYLYRGAYWRRGPVTMTAIAAVDMALWDIKAKAAGMPLYQLLGGKSRERVMTY
CCHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHEEEE
AHCTGQTIEDCLGEVARHVELGYRAVRVQSGVPGIETTYGVAKTPGERYEPADSSLPAEH
HHCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
VWSTEKYLNHAPKLFAAVRERFGDDLHVLHDVHHRLTPIEAARLGKAVEPYHLFWLEDCV
HHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEEECCCC
PAENQESLRLIREHTTTPLAIGEVFNSIHDCRELIQNQWIDYIRMPLTHGGGITAMRRVA
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
DLASLYHVRTGFHGPTDLSPVCLGAAIHFDTWVPNFGIQEHMPHTDETDAVFPHDYRFED
HHHHHHHHHCCCCCCCCCCHHHHHHEEEHHCCCCCCCCHHCCCCCCCCCCCCCCCCEECC
GHFLAGESPGHGVDIDEELAAKYPYERASLPVNRLEDGTLWHW
CCEECCCCCCCCCCCCHHHHHHCCCHHHCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7545940; 9097039; 9278503 [H]