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The map label for this gene is queA

Identifier: 92114949

GI number: 92114949

Start: 3161197

End: 3162267

Strand: Direct

Name: queA

Synonym: Csal_2832

Alternate gene names: 92114949

Gene position: 3161197-3162267 (Clockwise)

Preceding gene: 92114947

Following gene: 92114950

Centisome position: 85.52

GC content: 65.55

Gene sequence:

>1071_bases
ATGCAGCGCGCCGATTTCCATTTCGATCTCCCTGATGAGTTGATCGCCCGTTATCCGTCCGAGACCCGCAGCGATTGCCG
GCTGCTGTGCCTGGACGACGCTACCGGCGATATCGCTCACCGTCGCTTCACCGATCTGCTGACGCTTCTCGCGCCCGGCG
ATCTTCTCGTGTTCAACGACACCCGAGTCATCCCCGCCCGGCTGTTCGGCCAAAAGGCCAGCGGCGGGCGCGTCGAGATG
CTGCTGGAGCGGCCGCTGGACGCGCATCGTGGCCTCGCGCACCTGCGCGCCAGCAAGGCGCCCAGGCCCGGCACCGAGCT
GATCTTCGAAGGCGACGTGCACGCGGTCGTCGAAGCACGCCATGACGCCTTGTTCGAGCTGCGTTTCCTGGGCGAGACGC
CGATGATCGAGCTGCTCGAGAACCACGGCCACATGCCGCTGCCGCCTTATATCGACCGCGAGGACGAACTCGACGATCGC
GAGCGCTATCAGACGGTCTACGCGCGGCGCGAGGGCGCGGTCGCCGCGCCGACCGCCGGCTTGCACTTCGATACGCCGCT
GATGGAGGCATTGCGCGAACGCGGCGTGGAGATGGGCTATGTGACCCTGCACGTCGGCGCCGGCACCTTCCAGCCGGTGC
GCGTGGACGACATTCGCGAGCACCACATGCACAGCGAGTGGCTGGAGGTCGACGAGACGCTATGTCGGCAGGTCGAGGCG
ACCCGGGCGCGCGGCGGGCGCGTGATCGCCGTGGGCACGACCAGCGTGCGCTGCCTGGAAACCGCCAGCCAGGGTCAGGA
AGATGGCCACATCGCGCCCTTCCGCGGCGACACCGACATCTTCATCTATCCCGGCTATCAATGGCGGTGCGTCGACGCAC
TGATTACCAACTTCCATCTTCCCGAGTCGACGCTATTGATGCTGGTCTCGGCATTCGCGGGCTATCCCCAGGTGATGCGC
GCCTATCGCGCCGCCGTCGCCGAGCGGTATGCCTTCTTCAGTTATGGCGATGCCATGTTCCTGAATCGGTCGGCGTCCTC
ACATCCCTTTCCCGAGAATTCTCATGCGTAA

Upstream 100 bases:

>100_bases
CGCGTCTCGACTATCATAGGCCGCCTTCGTCGAGCGCACGCCGAGCGTGGCTCATTTCCGATGTCCGTTTTCCGATGCCA
GGTCGTTCCCTTCAGCCCCC

Downstream 100 bases:

>100_bases
CGAATGTTTCATGCGTTTCGAGCGCCTGGCCCAGGATGGACAGGCCCGGCGCGGTCGCCTTTCCTTTCCGCGCGGCACGG
TGGAGACGCCCGCCTTCATG

Product: S-adenosylmethionine

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MQRADFHFDLPDELIARYPSETRSDCRLLCLDDATGDIAHRRFTDLLTLLAPGDLLVFNDTRVIPARLFGQKASGGRVEM
LLERPLDAHRGLAHLRASKAPRPGTELIFEGDVHAVVEARHDALFELRFLGETPMIELLENHGHMPLPPYIDREDELDDR
ERYQTVYARREGAVAAPTAGLHFDTPLMEALRERGVEMGYVTLHVGAGTFQPVRVDDIREHHMHSEWLEVDETLCRQVEA
TRARGGRVIAVGTTSVRCLETASQGQEDGHIAPFRGDTDIFIYPGYQWRCVDALITNFHLPESTLLMLVSAFAGYPQVMR
AYRAAVAERYAFFSYGDAMFLNRSASSHPFPENSHA

Sequences:

>Translated_356_residues
MQRADFHFDLPDELIARYPSETRSDCRLLCLDDATGDIAHRRFTDLLTLLAPGDLLVFNDTRVIPARLFGQKASGGRVEM
LLERPLDAHRGLAHLRASKAPRPGTELIFEGDVHAVVEARHDALFELRFLGETPMIELLENHGHMPLPPYIDREDELDDR
ERYQTVYARREGAVAAPTAGLHFDTPLMEALRERGVEMGYVTLHVGAGTFQPVRVDDIREHHMHSEWLEVDETLCRQVEA
TRARGGRVIAVGTTSVRCLETASQGQEDGHIAPFRGDTDIFIYPGYQWRCVDALITNFHLPESTLLMLVSAFAGYPQVMR
AYRAAVAERYAFFSYGDAMFLNRSASSHPFPENSHA
>Mature_356_residues
MQRADFHFDLPDELIARYPSETRSDCRLLCLDDATGDIAHRRFTDLLTLLAPGDLLVFNDTRVIPARLFGQKASGGRVEM
LLERPLDAHRGLAHLRASKAPRPGTELIFEGDVHAVVEARHDALFELRFLGETPMIELLENHGHMPLPPYIDREDELDDR
ERYQTVYARREGAVAAPTAGLHFDTPLMEALRERGVEMGYVTLHVGAGTFQPVRVDDIREHHMHSEWLEVDETLCRQVEA
TRARGGRVIAVGTTSVRCLETASQGQEDGHIAPFRGDTDIFIYPGYQWRCVDALITNFHLPESTLLMLVSAFAGYPQVMR
AYRAAVAERYAFFSYGDAMFLNRSASSHPFPENSHA

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=344, Percent_Identity=64.5348837209302, Blast_Score=452, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_CHRSD (Q1QTN0)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_574877.1
- ProteinModelPortal:   Q1QTN0
- SMR:   Q1QTN0
- STRING:   Q1QTN0
- GeneID:   4028518
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_2832
- NMPDR:   fig|290398.4.peg.2304
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- BioCyc:   CSAL290398:CSAL_2832-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 40127; Mature: 40127

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRADFHFDLPDELIARYPSETRSDCRLLCLDDATGDIAHRRFTDLLTLLAPGDLLVFND
CCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECC
TRVIPARLFGQKASGGRVEMLLERPLDAHRGLAHLRASKAPRPGTELIFEGDVHAVVEAR
CEEEHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHH
HDALFELRFLGETPMIELLENHGHMPLPPYIDREDELDDRERYQTVYARREGAVAAPTAG
HHHEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEECCCCC
LHFDTPLMEALRERGVEMGYVTLHVGAGTFQPVRVDDIREHHMHSEWLEVDETLCRQVEA
CCCCCHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TRARGGRVIAVGTTSVRCLETASQGQEDGHIAPFRGDTDIFIYPGYQWRCVDALITNFHL
HHCCCCEEEEEECCCEEEHHHHHCCCCCCCCCCCCCCCCEEEECCCCEEEHHHHHHHCCC
PESTLLMLVSAFAGYPQVMRAYRAAVAERYAFFSYGDAMFLNRSASSHPFPENSHA
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MQRADFHFDLPDELIARYPSETRSDCRLLCLDDATGDIAHRRFTDLLTLLAPGDLLVFND
CCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECC
TRVIPARLFGQKASGGRVEMLLERPLDAHRGLAHLRASKAPRPGTELIFEGDVHAVVEAR
CEEEHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHH
HDALFELRFLGETPMIELLENHGHMPLPPYIDREDELDDRERYQTVYARREGAVAAPTAG
HHHEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEECCCCC
LHFDTPLMEALRERGVEMGYVTLHVGAGTFQPVRVDDIREHHMHSEWLEVDETLCRQVEA
CCCCCHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TRARGGRVIAVGTTSVRCLETASQGQEDGHIAPFRGDTDIFIYPGYQWRCVDALITNFHL
HHCCCCEEEEEECCCEEEHHHHHCCCCCCCCCCCCCCCCEEEECCCCEEEHHHHHHHCCC
PESTLLMLVSAFAGYPQVMRAYRAAVAERYAFFSYGDAMFLNRSASSHPFPENSHA
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA