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The map label for this gene is ycgF [H]

Identifier: 92114947

GI number: 92114947

Start: 3157335

End: 3158096

Strand: Direct

Name: ycgF [H]

Synonym: Csal_2830

Alternate gene names: 92114947

Gene position: 3157335-3158096 (Clockwise)

Preceding gene: 92114944

Following gene: 92114949

Centisome position: 85.41

GC content: 62.2

Gene sequence:

>762_bases
ATGACCCATTGCGCGCGCACCGACCATCACTGCAAGCGTTGCGACCATCCCTTGCCCTTCGCTTTCAGCATGGCCTTTCA
GCCCATCGTCGATGTCGCCAGGCAACAGGTCATCACCCACGAGGCCCTGGTCCGCGGTCTCGGTGACGAATCGGCCTACG
GCGTGCTCTCCCAGGTATCCGACAACCTGCTCTATCGCTTCGACCAGGCCTGTCGCGTCAAGGCCATCGAGCTGGCCAGC
GGGCTGGACATGAAAAGCGATTTGTCGATCAACTTCCTGCCCAATGCCGTCTACGCCCCCGAAGCCTGCATCCAGGCCAC
GCTGGACGTCTCCCGACGCGTGGGGTGGCCCACCGAACGCCTGGTCTTCGAAATCACCGAAACCGAGAGCGTGAACGATC
GCGGGCACCTGCGCAGCATCATCGACACCTACCGGCAGATGGGATTCACCACCGCCCTGGACGACTTCGGCAAGGGTCAT
GCCAACCTCGACCTGCTCGCCGACCTGCGCCCCGACAAGCTCAAGATCGATCGCGCACTGGTCATGGGCTGTCATCAGGA
CACACGCCGCCAGATATTGCTCAAGGCGGTCGCCGATATTGCCACGCAACTGGAGATCGACCTCGTGGCCGAAGGCATCG
AATGCCGGGAGGAAGCCAAGTGGCTGCATGAAGAAGCCGGCATCGTGCAGCATCAGGGCTTCTATTACGCGCGCCCGGCG
TTCGAATCCATGCCCACCGTCGCGGGATTCGCCACACCATAA

Upstream 100 bases:

>100_bases
GATAGAAGACACCCTATCTTAAACGACGCCCGCCACGCCCAACACCCATGGCGCGTCTGGCATCGTGCAGGGGTCGAGAC
ACGAGAACATGGAGGTAGGC

Downstream 100 bases:

>100_bases
GACAAAACGCCCCGACCATGGGGCCGGGGCGTTCATCCTGAGTTGCCGAAAACGCGATCAGGCGTCTTCAGGCAAGGCGT
AGGCGATCACGTAGTCGCCA

Product: diguanylate phosphodiesterase

Products: NA

Alternate protein names: Blrp [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MTHCARTDHHCKRCDHPLPFAFSMAFQPIVDVARQQVITHEALVRGLGDESAYGVLSQVSDNLLYRFDQACRVKAIELAS
GLDMKSDLSINFLPNAVYAPEACIQATLDVSRRVGWPTERLVFEITETESVNDRGHLRSIIDTYRQMGFTTALDDFGKGH
ANLDLLADLRPDKLKIDRALVMGCHQDTRRQILLKAVADIATQLEIDLVAEGIECREEAKWLHEEAGIVQHQGFYYARPA
FESMPTVAGFATP

Sequences:

>Translated_253_residues
MTHCARTDHHCKRCDHPLPFAFSMAFQPIVDVARQQVITHEALVRGLGDESAYGVLSQVSDNLLYRFDQACRVKAIELAS
GLDMKSDLSINFLPNAVYAPEACIQATLDVSRRVGWPTERLVFEITETESVNDRGHLRSIIDTYRQMGFTTALDDFGKGH
ANLDLLADLRPDKLKIDRALVMGCHQDTRRQILLKAVADIATQLEIDLVAEGIECREEAKWLHEEAGIVQHQGFYYARPA
FESMPTVAGFATP
>Mature_252_residues
THCARTDHHCKRCDHPLPFAFSMAFQPIVDVARQQVITHEALVRGLGDESAYGVLSQVSDNLLYRFDQACRVKAIELASG
LDMKSDLSINFLPNAVYAPEACIQATLDVSRRVGWPTERLVFEITETESVNDRGHLRSIIDTYRQMGFTTALDDFGKGHA
NLDLLADLRPDKLKIDRALVMGCHQDTRRQILLKAVADIATQLEIDLVAEGIECREEAKWLHEEAGIVQHQGFYYARPAF
ESMPTVAGFATP

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787410, Length=226, Percent_Identity=30.0884955752212, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI87081845, Length=166, Percent_Identity=33.1325301204819, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1790496, Length=228, Percent_Identity=27.1929824561404, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI226510982, Length=231, Percent_Identity=26.4069264069264, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788502, Length=236, Percent_Identity=27.9661016949153, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI87081980, Length=123, Percent_Identity=34.1463414634146, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787541, Length=125, Percent_Identity=34.4, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787055, Length=231, Percent_Identity=26.8398268398268, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI87081743, Length=225, Percent_Identity=28, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081921, Length=149, Percent_Identity=31.5436241610738, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI87082096, Length=121, Percent_Identity=29.7520661157025, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1786507, Length=133, Percent_Identity=30.8270676691729, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633 [H]

Pfam domain/function: PF04940 BLUF; PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 28343; Mature: 28211

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHCARTDHHCKRCDHPLPFAFSMAFQPIVDVARQQVITHEALVRGLGDESAYGVLSQVS
CCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
DNLLYRFDQACRVKAIELASGLDMKSDLSINFLPNAVYAPEACIQATLDVSRRVGWPTER
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHH
LVFEITETESVNDRGHLRSIIDTYRQMGFTTALDDFGKGHANLDLLADLRPDKLKIDRAL
EEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECCCCCHHHHHHHH
VMGCHQDTRRQILLKAVADIATQLEIDLVAEGIECREEAKWLHEEAGIVQHQGFYYARPA
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEECCEEEECCH
FESMPTVAGFATP
HHHCCCCCCCCCC
>Mature Secondary Structure 
THCARTDHHCKRCDHPLPFAFSMAFQPIVDVARQQVITHEALVRGLGDESAYGVLSQVS
CCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
DNLLYRFDQACRVKAIELASGLDMKSDLSINFLPNAVYAPEACIQATLDVSRRVGWPTER
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHH
LVFEITETESVNDRGHLRSIIDTYRQMGFTTALDDFGKGHANLDLLADLRPDKLKIDRAL
EEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECCCCCHHHHHHHH
VMGCHQDTRRQILLKAVADIATQLEIDLVAEGIECREEAKWLHEEAGIVQHQGFYYARPA
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEECCEEEECCH
FESMPTVAGFATP
HHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]