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The map label for this gene is tgt

Identifier: 92114950

GI number: 92114950

Start: 3162278

End: 3163396

Strand: Direct

Name: tgt

Synonym: Csal_2833

Alternate gene names: 92114950

Gene position: 3162278-3163396 (Clockwise)

Preceding gene: 92114949

Following gene: 92114951

Centisome position: 85.54

GC content: 64.34

Gene sequence:

>1119_bases
ATGCGTTTCGAGCGCCTGGCCCAGGATGGACAGGCCCGGCGCGGTCGCCTTTCCTTTCCGCGCGGCACGGTGGAGACGCC
CGCCTTCATGCCGGTGGGCACCTACGGCACGGTCAAGGGCATGACGCCCCAGTCGGTGAAGGACATCGGTGCCGAGATCA
TTCTCGGCAATACCTTTCATCTATGGCTGCGCCCCGGCACCGAGGTCATCGAGACGCACGGCGATCTGCATGACTTCGCC
CAGTGGGACAAGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTTTCGCTGGGCGACATGCGCAAGATCACCGAGGA
AGGCGTGCACTTCCGCTCCCCCGTGGATGGCGCCAAGGTGTTCATGGGTCCCGAGGAATCGATGGCCGTGCAGCGCTCGC
TGGGTTCGGACATCGTGATGATCTTCGACGAGTGCACGCCCTACCCGGCCACCGAGGCAGAGGCCAAGCGCTCCATGGAG
ATGTCGCTGCGCTGGGCCGAGCGCTCGCGCATCGCGCACGGCGACTCGCCCTCGGCGCTGTTCGGCATCATCCAGGGCGG
CATGTACCCCGAGCTTCGCGAGCGCTCCCTGAAGGGGCTCTTGGACATCGGCTTCGATGGGCTCGCCATCGGCGGTTTGT
CCGTGGGCGAACCCAAGGAAGAGATGCTCAAGGTACTCGACTATTTGCCGGGCTGGATGCCCGACGACAAGCCCCGTTAC
CTGATGGGCGTCGGCAAGCCCGAGGACCTGGTCGAGGGCGTGCGGCGCGGGGTGGACATGTTCGACTGCGTGATGCCCAC
GCGCAACGCGCGCAACGGGTATCTGTTCACCGCCGAGGGCACCGTCAAGATCCGCAATGCCCAGCACCGTTACTCGACAC
AGGCACTCGAGGCGGATTGCGATTGTCATACCTGCCAACACTTCTCGCGCAGCTATCTCCACCACCTGGACCGCTGCGGG
GAAATGCTCGGCGCGATGCTCAATACCATCCACAATCTGCGCTATTACCAGCGCGTGATGGCTGGTTTGCGCACTGCCAT
TGAAGCGGGTACATTGACTGCCTTTGTCGAGGACTTCTACGCACGGCGAGGCATGCCGGTGCCGCCGCTGGCAGCGTGA

Upstream 100 bases:

>100_bases
GAGCGGTATGCCTTCTTCAGTTATGGCGATGCCATGTTCCTGAATCGGTCGGCGTCCTCACATCCCTTTCCCGAGAATTC
TCATGCGTAACGAATGTTTC

Downstream 100 bases:

>100_bases
ACGGACACCGTCGAGGCCTTGTCTCGCGCACTCACTTTTCACCCCACCACTATCCGGAGATAACAATCCATGCTGGAGTT
TTTCATTCCCGCAGCTCATG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MRFERLAQDGQARRGRLSFPRGTVETPAFMPVGTYGTVKGMTPQSVKDIGAEIILGNTFHLWLRPGTEVIETHGDLHDFA
QWDKPILTDSGGFQVFSLGDMRKITEEGVHFRSPVDGAKVFMGPEESMAVQRSLGSDIVMIFDECTPYPATEAEAKRSME
MSLRWAERSRIAHGDSPSALFGIIQGGMYPELRERSLKGLLDIGFDGLAIGGLSVGEPKEEMLKVLDYLPGWMPDDKPRY
LMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGYLFTAEGTVKIRNAQHRYSTQALEADCDCHTCQHFSRSYLHHLDRCG
EMLGAMLNTIHNLRYYQRVMAGLRTAIEAGTLTAFVEDFYARRGMPVPPLAA

Sequences:

>Translated_372_residues
MRFERLAQDGQARRGRLSFPRGTVETPAFMPVGTYGTVKGMTPQSVKDIGAEIILGNTFHLWLRPGTEVIETHGDLHDFA
QWDKPILTDSGGFQVFSLGDMRKITEEGVHFRSPVDGAKVFMGPEESMAVQRSLGSDIVMIFDECTPYPATEAEAKRSME
MSLRWAERSRIAHGDSPSALFGIIQGGMYPELRERSLKGLLDIGFDGLAIGGLSVGEPKEEMLKVLDYLPGWMPDDKPRY
LMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGYLFTAEGTVKIRNAQHRYSTQALEADCDCHTCQHFSRSYLHHLDRCG
EMLGAMLNTIHNLRYYQRVMAGLRTAIEAGTLTAFVEDFYARRGMPVPPLAA
>Mature_372_residues
MRFERLAQDGQARRGRLSFPRGTVETPAFMPVGTYGTVKGMTPQSVKDIGAEIILGNTFHLWLRPGTEVIETHGDLHDFA
QWDKPILTDSGGFQVFSLGDMRKITEEGVHFRSPVDGAKVFMGPEESMAVQRSLGSDIVMIFDECTPYPATEAEAKRSME
MSLRWAERSRIAHGDSPSALFGIIQGGMYPELRERSLKGLLDIGFDGLAIGGLSVGEPKEEMLKVLDYLPGWMPDDKPRY
LMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGYLFTAEGTVKIRNAQHRYSTQALEADCDCHTCQHFSRSYLHHLDRCG
EMLGAMLNTIHNLRYYQRVMAGLRTAIEAGTLTAFVEDFYARRGMPVPPLAA

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=360, Percent_Identity=42.2222222222222, Blast_Score=289, Evalue=3e-78,
Organism=Escherichia coli, GI1786607, Length=372, Percent_Identity=69.6236559139785, Blast_Score=549, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI17544680, Length=361, Percent_Identity=40.9972299168975, Blast_Score=281, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI25146502, Length=138, Percent_Identity=27.536231884058, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19920510, Length=362, Percent_Identity=42.5414364640884, Blast_Score=315, Evalue=3e-86,
Organism=Drosophila melanogaster, GI21355311, Length=258, Percent_Identity=24.031007751938, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_CHRSD (Q1QTM9)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_574878.1
- ProteinModelPortal:   Q1QTM9
- SMR:   Q1QTM9
- STRING:   Q1QTM9
- GeneID:   4028519
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_2833
- NMPDR:   fig|290398.4.peg.2305
- eggNOG:   COG0343
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- BioCyc:   CSAL290398:CSAL_2833-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 41489; Mature: 41489

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: ACT_SITE 89-89 BINDING 90-90

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFERLAQDGQARRGRLSFPRGTVETPAFMPVGTYGTVKGMTPQSVKDIGAEIILGNTFH
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCHHHHHHCCCEEEECCEEE
LWLRPGTEVIETHGDLHDFAQWDKPILTDSGGFQVFSLGDMRKITEEGVHFRSPVDGAKV
EEECCCHHHHHHCCCHHHHHHCCCCEEECCCCEEEEECCHHHHHHHCCCEECCCCCCCEE
FMGPEESMAVQRSLGSDIVMIFDECTPYPATEAEAKRSMEMSLRWAERSRIAHGDSPSAL
EECCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
FGIIQGGMYPELRERSLKGLLDIGFDGLAIGGLSVGEPKEEMLKVLDYLPGWMPDDKPRY
HHHHHCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCE
LMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGYLFTAEGTVKIRNAQHRYSTQALEADC
EEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCEEEECCCHHHHHHHHHHCCC
DCHTCQHFSRSYLHHLDRCGEMLGAMLNTIHNLRYYQRVMAGLRTAIEAGTLTAFVEDFY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARRGMPVPPLAA
HHCCCCCCCCCC
>Mature Secondary Structure
MRFERLAQDGQARRGRLSFPRGTVETPAFMPVGTYGTVKGMTPQSVKDIGAEIILGNTFH
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCHHHHHHCCCEEEECCEEE
LWLRPGTEVIETHGDLHDFAQWDKPILTDSGGFQVFSLGDMRKITEEGVHFRSPVDGAKV
EEECCCHHHHHHCCCHHHHHHCCCCEEECCCCEEEEECCHHHHHHHCCCEECCCCCCCEE
FMGPEESMAVQRSLGSDIVMIFDECTPYPATEAEAKRSMEMSLRWAERSRIAHGDSPSAL
EECCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
FGIIQGGMYPELRERSLKGLLDIGFDGLAIGGLSVGEPKEEMLKVLDYLPGWMPDDKPRY
HHHHHCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCE
LMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGYLFTAEGTVKIRNAQHRYSTQALEADC
EEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCEEEECCCHHHHHHHHHHCCC
DCHTCQHFSRSYLHHLDRCGEMLGAMLNTIHNLRYYQRVMAGLRTAIEAGTLTAFVEDFY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARRGMPVPPLAA
HHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA