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The map label for this gene is purB [H]

Identifier: 92114564

GI number: 92114564

Start: 2750596

End: 2751960

Strand: Direct

Name: purB [H]

Synonym: Csal_2446

Alternate gene names: 92114564

Gene position: 2750596-2751960 (Clockwise)

Preceding gene: 92114563

Following gene: 92114565

Centisome position: 74.41

GC content: 63.44

Gene sequence:

>1365_bases
ATGGAATTGTCCGCCCTCACCGCCCTGTCCCCCGTCGATGGCCGCTATGGCGCCAAAGCCGCCGCCTTGCGCGAACATTT
CAGCGAATACGGCTTGATCCGGGCTCGCGTCACCGTCGAGGTGCGCTGGCTGGAGCGTCTCGCCGCGCATCCGGGGATCG
TCGAGGTGCCACCGCTCTCGGCCCAGGCCACCAACTTCCTGAGCCAGCTGGTGAGCGAGTTCTCGGTCGCCGACGCCGAG
CGCATCAAGGACATCGAGCGCACCACCAATCACGACGTCAAGGCGGTGGAGTACTTCCTCAAGGAGAAGGTCGCCGAGGT
CGACGAGCTTCACGCGATCACCGAATTCATCCACTTCGCCTGCACCAGCGAGGACATCAACAACCTGTCCTACGGCGTGA
TGCTGCGTGACGGACTCGACACGCTGCTGCCGACGCTGCGCGAAATCGTCACCGCCATCACGCGCCTGGCCCACGAGCAT
GCCGAGCAGCCCATGCTCTCGCGTACCCACGGCCAGACCGCCAGCCCCACCACGCTGGGCAAGGAAATGGCCAACGTGGC
ATACCGCCTGCGCCGCCAGCTCAAGCAGATCGAGCAAGCCGAAATCCTCGGCAAGATCAACGGCGCGGTAGGCAACTACA
ATGCCCACCTGGCCACCTACCCCGAGATCGACTGGGCCGAGAATGCGCGCATCTTCGTCGAAGGCCTGGGGCTGACCTTC
AACCCCTACACCACGCAGATCGAGCCGCACGACTACATCGCCGAACTGTTCGACGCCATCGCGCGCTTCAACACCATCCT
GATCGACTTCGACCGCGACGTGTGGGGCTACATTTCGCTGGGCTACTTCAAGCAGAAGACCGTCGAGGGGGAAGTCGGCT
CCTCGACCATGCCCCACAAGGTCAATCCCATCGATTTCGAGAACTCCGAGGGCAATCTCGGCCTCTCCAACGCGCTGCTC
GGTCACCTCGCCCAGAAACTGCCGATCTCCCGCTGGCAGCGCGATCTGACCGACTCCACGGTGCTGCGCAACCTCGGCGT
GGGTCTGGCGTACGGCTTGATCGCCTATCAGGCCACCCTCAAGGGCATCGGCAAGCTGGAAGCCAATCCCGCACGTCTCG
ACGAGGACCTCGACCAGAACTGGGAAGTCCTCGCCGAGCCGATCCAGACCGTGATGCGCCGTTACGGCATCGAGAAACCC
TACGAGAAGCTCAAGACGCTGACGCGTGGCAAGCGCGTCGATCAGGCCGGCTTCGCGGCCTTCATCGACACTCTGGAACT
GCCCGACGACGTCAAGACCGAGCTCAAGGCACTGACCCCGGCCACCTATATCGGGAACGCCAGCGAGCAGGCTCGCAATC
TATAA

Upstream 100 bases:

>100_bases
GCTGTTTCAACGCCAGGGCATCAAGAAGTCCCTGGCCGCTCTAGATGGCCACTGAGCGCTGCGCCGCGCGATTCGATTCA
CAATCAGGAAGACGACACCC

Downstream 100 bases:

>100_bases
GCGGGAAGCGCCGACATCACCAGGTAAACGGCACCGGGGACAGGCCGAAGCGGAGGCGTACAGGGAGGCTTTGACAGCGC
CCCCCTCGCGCAGGCCTGTC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MELSALTALSPVDGRYGAKAAALREHFSEYGLIRARVTVEVRWLERLAAHPGIVEVPPLSAQATNFLSQLVSEFSVADAE
RIKDIERTTNHDVKAVEYFLKEKVAEVDELHAITEFIHFACTSEDINNLSYGVMLRDGLDTLLPTLREIVTAITRLAHEH
AEQPMLSRTHGQTASPTTLGKEMANVAYRLRRQLKQIEQAEILGKINGAVGNYNAHLATYPEIDWAENARIFVEGLGLTF
NPYTTQIEPHDYIAELFDAIARFNTILIDFDRDVWGYISLGYFKQKTVEGEVGSSTMPHKVNPIDFENSEGNLGLSNALL
GHLAQKLPISRWQRDLTDSTVLRNLGVGLAYGLIAYQATLKGIGKLEANPARLDEDLDQNWEVLAEPIQTVMRRYGIEKP
YEKLKTLTRGKRVDQAGFAAFIDTLELPDDVKTELKALTPATYIGNASEQARNL

Sequences:

>Translated_454_residues
MELSALTALSPVDGRYGAKAAALREHFSEYGLIRARVTVEVRWLERLAAHPGIVEVPPLSAQATNFLSQLVSEFSVADAE
RIKDIERTTNHDVKAVEYFLKEKVAEVDELHAITEFIHFACTSEDINNLSYGVMLRDGLDTLLPTLREIVTAITRLAHEH
AEQPMLSRTHGQTASPTTLGKEMANVAYRLRRQLKQIEQAEILGKINGAVGNYNAHLATYPEIDWAENARIFVEGLGLTF
NPYTTQIEPHDYIAELFDAIARFNTILIDFDRDVWGYISLGYFKQKTVEGEVGSSTMPHKVNPIDFENSEGNLGLSNALL
GHLAQKLPISRWQRDLTDSTVLRNLGVGLAYGLIAYQATLKGIGKLEANPARLDEDLDQNWEVLAEPIQTVMRRYGIEKP
YEKLKTLTRGKRVDQAGFAAFIDTLELPDDVKTELKALTPATYIGNASEQARNL
>Mature_454_residues
MELSALTALSPVDGRYGAKAAALREHFSEYGLIRARVTVEVRWLERLAAHPGIVEVPPLSAQATNFLSQLVSEFSVADAE
RIKDIERTTNHDVKAVEYFLKEKVAEVDELHAITEFIHFACTSEDINNLSYGVMLRDGLDTLLPTLREIVTAITRLAHEH
AEQPMLSRTHGQTASPTTLGKEMANVAYRLRRQLKQIEQAEILGKINGAVGNYNAHLATYPEIDWAENARIFVEGLGLTF
NPYTTQIEPHDYIAELFDAIARFNTILIDFDRDVWGYISLGYFKQKTVEGEVGSSTMPHKVNPIDFENSEGNLGLSNALL
GHLAQKLPISRWQRDLTDSTVLRNLGVGLAYGLIAYQATLKGIGKLEANPARLDEDLDQNWEVLAEPIQTVMRRYGIEKP
YEKLKTLTRGKRVDQAGFAAFIDTLELPDDVKTELKALTPATYIGNASEQARNL

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=448, Percent_Identity=71.2053571428571, Blast_Score=673, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 50690; Mature: 50690

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSALTALSPVDGRYGAKAAALREHFSEYGLIRARVTVEVRWLERLAAHPGIVEVPPLS
CCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCEEEEEEEEHHHHHHHHHCCCCCEECCCCC
AQATNFLSQLVSEFSVADAERIKDIERTTNHDVKAVEYFLKEKVAEVDELHAITEFIHFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CTSEDINNLSYGVMLRDGLDTLLPTLREIVTAITRLAHEHAEQPMLSRTHGQTASPTTLG
CCCHHHCCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHH
KEMANVAYRLRRQLKQIEQAEILGKINGAVGNYNAHLATYPEIDWAENARIFVEGLGLTF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCEEEEEECCEEE
NPYTTQIEPHDYIAELFDAIARFNTILIDFDRDVWGYISLGYFKQKTVEGEVGSSTMPHK
CCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
VNPIDFENSEGNLGLSNALLGHLAQKLPISRWQRDLTDSTVLRNLGVGLAYGLIAYQATL
CCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGIGKLEANPARLDEDLDQNWEVLAEPIQTVMRRYGIEKPYEKLKTLTRGKRVDQAGFAA
HHHHCCCCCHHHCCHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHH
FIDTLELPDDVKTELKALTPATYIGNASEQARNL
HHHHHCCCHHHHHHHHHHCCHHHCCCCHHHHCCC
>Mature Secondary Structure
MELSALTALSPVDGRYGAKAAALREHFSEYGLIRARVTVEVRWLERLAAHPGIVEVPPLS
CCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCEEEEEEEEHHHHHHHHHCCCCCEECCCCC
AQATNFLSQLVSEFSVADAERIKDIERTTNHDVKAVEYFLKEKVAEVDELHAITEFIHFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CTSEDINNLSYGVMLRDGLDTLLPTLREIVTAITRLAHEHAEQPMLSRTHGQTASPTTLG
CCCHHHCCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHH
KEMANVAYRLRRQLKQIEQAEILGKINGAVGNYNAHLATYPEIDWAENARIFVEGLGLTF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCEEEEEECCEEE
NPYTTQIEPHDYIAELFDAIARFNTILIDFDRDVWGYISLGYFKQKTVEGEVGSSTMPHK
CCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
VNPIDFENSEGNLGLSNALLGHLAQKLPISRWQRDLTDSTVLRNLGVGLAYGLIAYQATL
CCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGIGKLEANPARLDEDLDQNWEVLAEPIQTVMRRYGIEKPYEKLKTLTRGKRVDQAGFAA
HHHHCCCCCHHHCCHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHH
FIDTLELPDDVKTELKALTPATYIGNASEQARNL
HHHHHCCCHHHHHHHHHHCCHHHCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]