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The map label for this gene is tapD [H]

Identifier: 92114298

GI number: 92114298

Start: 2450178

End: 2451020

Strand: Direct

Name: tapD [H]

Synonym: Csal_2176

Alternate gene names: 92114298

Gene position: 2450178-2451020 (Clockwise)

Preceding gene: 92114297

Following gene: 92114299

Centisome position: 66.28

GC content: 66.31

Gene sequence:

>843_bases
TTGTTGGCCGATATCCCCCTGCCCTGGCTGGCCGTGATGGCTTTCGTCGTGGGTACGCTGCTGGGCAGCTTTCTCAATGT
CGTCATCGTGCGCCTGCCGGTCATGCTGATGCAGCAATGGCGTCAGGAGGCGCGCGAATCGCTGGCCCTCGAGACGGAGG
ACGTGCCGGCCTACAACCTGTGCACGCCACGCTCGCAATGTCCGCGTTGCCGAGCGCCGATCGCCTGGCACGACAATCTG
CCCTTGATCGGCTGGCTCAAGCGACGCGGTCGCTGTGCGCATTGCCAGGGGCGGATCAGCGCACAATATCCTCTGGTCGA
GCTGGCCGCTGGGCTGTTGCTGTTCAGCGTGGTGATGGTGCATGGCGCGACCTGGTCCGCCCTCGCCTGGGGCGGGCTGT
GCCTGACGCTGCTGGTGGTCGCCGTCATCGACCTGCGCACGCAGCTGCTGCCCGATATCATCACGCTCCCCCTGCTCTGG
GCGGGGCTTATCTACCAGCTCGTCTTTCAGCCCTATTGGCTGAGCAACGCCGTCATCGGCGCGCTCGCCGGCTATCTGCT
GCTCTGGGGGTTCTACTGGCTCTTCAAGTGGATCACCGGCAAGGAGGGCATGGGGTACGGCGACTTCAAGCTGCTGGCGG
CGCTGGGGGCCTGGTTCGGCTGGACGATGCTGCCGATTCTGCTGATCCTTTCCGCCGGCCTGGGAGCCGTCATCGGTGGG
CTATTGCAACTCACGCACCCCCGGCTACGCGGCATGCCGCTGCCCTTCGGCCCCTTCCTCGCCCTCGCCGGCTGGGTGGC
GGTCCTCTTCGGCGCACCGCTGCGCGCCTTGACCGGTCTATGA

Upstream 100 bases:

>100_bases
CGCGCTCGCCCGCGCCGGCGTGACACCGGCCCGTTCGCGCCCACTTCTGCCTGTCCATCCCGAAGGCAGGCATTTTCAGA
CATAGCGTAGGAGTTGCAAG

Downstream 100 bases:

>100_bases
GGCACTCGACATGACCACACCCGTACGCATCATCGGGGTGACCGGCGGCATCGCCTCGGGCAAGTCCACCGTCGCCGCCA
TGTTCGCCGAACACGGTATT

Product: type 4 prepilin peptidase 1

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MLADIPLPWLAVMAFVVGTLLGSFLNVVIVRLPVMLMQQWRQEARESLALETEDVPAYNLCTPRSQCPRCRAPIAWHDNL
PLIGWLKRRGRCAHCQGRISAQYPLVELAAGLLLFSVVMVHGATWSALAWGGLCLTLLVVAVIDLRTQLLPDIITLPLLW
AGLIYQLVFQPYWLSNAVIGALAGYLLLWGFYWLFKWITGKEGMGYGDFKLLAALGAWFGWTMLPILLILSAGLGAVIGG
LLQLTHPRLRGMPLPFGPFLALAGWVAVLFGAPLRALTGL

Sequences:

>Translated_280_residues
MLADIPLPWLAVMAFVVGTLLGSFLNVVIVRLPVMLMQQWRQEARESLALETEDVPAYNLCTPRSQCPRCRAPIAWHDNL
PLIGWLKRRGRCAHCQGRISAQYPLVELAAGLLLFSVVMVHGATWSALAWGGLCLTLLVVAVIDLRTQLLPDIITLPLLW
AGLIYQLVFQPYWLSNAVIGALAGYLLLWGFYWLFKWITGKEGMGYGDFKLLAALGAWFGWTMLPILLILSAGLGAVIGG
LLQLTHPRLRGMPLPFGPFLALAGWVAVLFGAPLRALTGL
>Mature_280_residues
MLADIPLPWLAVMAFVVGTLLGSFLNVVIVRLPVMLMQQWRQEARESLALETEDVPAYNLCTPRSQCPRCRAPIAWHDNL
PLIGWLKRRGRCAHCQGRISAQYPLVELAAGLLLFSVVMVHGATWSALAWGGLCLTLLVVAVIDLRTQLLPDIITLPLLW
AGLIYQLVFQPYWLSNAVIGALAGYLLLWGFYWLFKWITGKEGMGYGDFKLLAALGAWFGWTMLPILLILSAGLGAVIGG
LLQLTHPRLRGMPLPFGPFLALAGWVAVLFGAPLRALTGL

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the tapA pilin precursor during membrane translocation [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=266, Percent_Identity=38.7218045112782, Blast_Score=150, Evalue=9e-38,
Organism=Escherichia coli, GI1789732, Length=129, Percent_Identity=50.3875968992248, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 30809; Mature: 30809

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure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HHHHHCHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA