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The map label for this gene is mltD [H]

Identifier: 92114068

GI number: 92114068

Start: 2200030

End: 2201367

Strand: Direct

Name: mltD [H]

Synonym: Csal_1945

Alternate gene names: 92114068

Gene position: 2200030-2201367 (Clockwise)

Preceding gene: 92114067

Following gene: 92114069

Centisome position: 59.51

GC content: 67.86

Gene sequence:

>1338_bases
ATGAAAGTACGATCGAGTCGCCGCCAGGCGCTACGTTTGGGTCACGTAGCCCTGGCGGGAGGACTGTTATTGAGCGCATT
GTCGCTTCCCGCCGCGAAGGCCGCCTCCGGCGAGGCCACCGGCACCTCGGCAGCCACGACTACCTTCTGGCAAGCCCTGG
TATTGCAGCGCGCCCCGCAGGCCGATGCCTGGACGCGCCTCAAGCAGGGCTTCGGCCTGCCGCACGATATTCAGCATCCG
CGTGTCGCGAAATGGCTCGACTGGTATGCCGCGCACCCCGAGCACGTCGAGCAGGTCGCCGCCAAGGCGCGCCCCTGGCT
GCGCTGGGTGACGCGTCAGCTCGAGGCTCGCGACATGCCCACCGAAATCGCCCTGCTGCCTTTCATCGAAAGCGCCTACG
ACCCCACGGCCCGCCACCCCGGCGGTGCTTCCGGGCTCTGGCAGTTCATGCCCGGCACCGGCGAAGCCATGGGGCTCGCC
GAGACCTGGTGGTACGACGGGCGCCGCGATGTCGTCTCTTCGACGAATGCGGCCCTCGACTACCTGCAGACGCTCGCCGA
CCAGTGGTACGAAGGCAACATGGAACTCGCCCTCGCCGCCTACAATGCCGGCGCCGGCACCGTCAATCGCGCGCGTGAGC
GAGCCGCCGCGCAGGGCAAGCCCATCGACTACTGGCACCTCTCGCTGCCCGCCCAGACCATGGACTACGTGCCCAAGCTT
CTCGCCTTGTCGGCGGTCATCTCGGACCCCCAGCGGTACGCCATCGACCTGCCGAGCATTCCCGATCGACCGGCCATCGC
CAGGGTCACCGCCGAGGGGCAACTGTCGCTGGCCGACGCCGCCCAGCTCGCCGATGTCAGCGAAGCGCGGCTGCAAGCCC
TCAACCCCGGCCTGCGGCGTGGTGCCACGCGCCCCGACAGGAACGCGCAATTGATCGTGCCCGCCGATCGCCGGCAGCGT
TTTCTCGCCAATCTCGATACGCTGCCCGCCGAAAAACGGCGTACCCGGACGCACTACGTGGTCCAGTCCGGCGACACGCT
GTCCGGCATTGCCGCGCGCCATGACATCCCGGTGGGCGTACTGCGTCGGCAGAATGGGATCAGCGGCAATATGCTGCGCG
TCGGTCAATCCCTGGTCATTCCGGGCGCGTCGAGCCGTGCACGCCTGGCCAGCCGTGAGGCCACCCAGCAAGTCCGCGTC
AAGCCGGGTGACAGCCTCTCGCGTATCGCGGCACGCCATGATGTCAGCGTCGACGACCTGACACGCTGGAATCAGATACA
GCGCGACGATTATCTCCAGCCCGGACAGCGTCTCACGCTTCGCACCCCGGCGCGTTGA

Upstream 100 bases:

>100_bases
ATGACGACAGTGCCGCCTTCGCGACACTACGCGGCTGGAAGGATCGCTTCTAGGCCATGGCCGGCTCGGCCGCTACTGTA
CCTCGAGAGGTTCGCTTCCC

Downstream 100 bases:

>100_bases
GCATCGTGGCGGCGTTGCCCTCATGCCCCGCCCCTGATCAGAGGGCGGGCGCGTGCGCCACCGGCGAGGCTGGGGTAGGC
TAGGCACGGCCGAAAACGGC

Product: lytic transglycosylase

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MKVRSSRRQALRLGHVALAGGLLLSALSLPAAKAASGEATGTSAATTTFWQALVLQRAPQADAWTRLKQGFGLPHDIQHP
RVAKWLDWYAAHPEHVEQVAAKARPWLRWVTRQLEARDMPTEIALLPFIESAYDPTARHPGGASGLWQFMPGTGEAMGLA
ETWWYDGRRDVVSSTNAALDYLQTLADQWYEGNMELALAAYNAGAGTVNRARERAAAQGKPIDYWHLSLPAQTMDYVPKL
LALSAVISDPQRYAIDLPSIPDRPAIARVTAEGQLSLADAAQLADVSEARLQALNPGLRRGATRPDRNAQLIVPADRRQR
FLANLDTLPAEKRRTRTHYVVQSGDTLSGIAARHDIPVGVLRRQNGISGNMLRVGQSLVIPGASSRARLASREATQQVRV
KPGDSLSRIAARHDVSVDDLTRWNQIQRDDYLQPGQRLTLRTPAR

Sequences:

>Translated_445_residues
MKVRSSRRQALRLGHVALAGGLLLSALSLPAAKAASGEATGTSAATTTFWQALVLQRAPQADAWTRLKQGFGLPHDIQHP
RVAKWLDWYAAHPEHVEQVAAKARPWLRWVTRQLEARDMPTEIALLPFIESAYDPTARHPGGASGLWQFMPGTGEAMGLA
ETWWYDGRRDVVSSTNAALDYLQTLADQWYEGNMELALAAYNAGAGTVNRARERAAAQGKPIDYWHLSLPAQTMDYVPKL
LALSAVISDPQRYAIDLPSIPDRPAIARVTAEGQLSLADAAQLADVSEARLQALNPGLRRGATRPDRNAQLIVPADRRQR
FLANLDTLPAEKRRTRTHYVVQSGDTLSGIAARHDIPVGVLRRQNGISGNMLRVGQSLVIPGASSRARLASREATQQVRV
KPGDSLSRIAARHDVSVDDLTRWNQIQRDDYLQPGQRLTLRTPAR
>Mature_445_residues
MKVRSSRRQALRLGHVALAGGLLLSALSLPAAKAASGEATGTSAATTTFWQALVLQRAPQADAWTRLKQGFGLPHDIQHP
RVAKWLDWYAAHPEHVEQVAAKARPWLRWVTRQLEARDMPTEIALLPFIESAYDPTARHPGGASGLWQFMPGTGEAMGLA
ETWWYDGRRDVVSSTNAALDYLQTLADQWYEGNMELALAAYNAGAGTVNRARERAAAQGKPIDYWHLSLPAQTMDYVPKL
LALSAVISDPQRYAIDLPSIPDRPAIARVTAEGQLSLADAAQLADVSEARLQALNPGLRRGATRPDRNAQLIVPADRRQR
FLANLDTLPAEKRRTRTHYVVQSGDTLSGIAARHDIPVGVLRRQNGISGNMLRVGQSLVIPGASSRARLASREATQQVRV
KPGDSLSRIAARHDVSVDDLTRWNQIQRDDYLQPGQRLTLRTPAR

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=422, Percent_Identity=36.7298578199052, Blast_Score=256, Evalue=2e-69,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 48906; Mature: 48906

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVRSSRRQALRLGHVALAGGLLLSALSLPAAKAASGEATGTSAATTTFWQALVLQRAPQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
ADAWTRLKQGFGLPHDIQHPRVAKWLDWYAAHPEHVEQVAAKARPWLRWVTRQLEARDMP
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TEIALLPFIESAYDPTARHPGGASGLWQFMPGTGEAMGLAETWWYDGRRDVVSSTNAALD
CHHHHHHHHHHCCCCCCCCCCCCCCHHEECCCCCCCCCCHHHHCCCCCHHHHHHHHHHHH
YLQTLADQWYEGNMELALAAYNAGAGTVNRARERAAAQGKPIDYWHLSLPAQTMDYVPKL
HHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHH
LALSAVISDPQRYAIDLPSIPDRPAIARVTAEGQLSLADAAQLADVSEARLQALNPGLRR
HHHHHHHCCCHHEEEECCCCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHCCHHHC
GATRPDRNAQLIVPADRRQRFLANLDTLPAEKRRTRTHYVVQSGDTLSGIAARHDIPVGV
CCCCCCCCCEEEEECHHHHHHHHHHCCCCCHHHHCCEEEEEECCCCHHHHHHHCCCCHHH
LRRQNGISGNMLRVGQSLVIPGASSRARLASREATQQVRVKPGDSLSRIAARHDVSVDDL
HCCCCCCCCCHHHCCCEEEECCCCCHHHHHHHHHHHHEECCCCCHHHHHHHHCCCCHHHH
TRWNQIQRDDYLQPGQRLTLRTPAR
HHHHHHCCCCCCCCCCEEEEECCCC
>Mature Secondary Structure
MKVRSSRRQALRLGHVALAGGLLLSALSLPAAKAASGEATGTSAATTTFWQALVLQRAPQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
ADAWTRLKQGFGLPHDIQHPRVAKWLDWYAAHPEHVEQVAAKARPWLRWVTRQLEARDMP
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TEIALLPFIESAYDPTARHPGGASGLWQFMPGTGEAMGLAETWWYDGRRDVVSSTNAALD
CHHHHHHHHHHCCCCCCCCCCCCCCHHEECCCCCCCCCCHHHHCCCCCHHHHHHHHHHHH
YLQTLADQWYEGNMELALAAYNAGAGTVNRARERAAAQGKPIDYWHLSLPAQTMDYVPKL
HHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHH
LALSAVISDPQRYAIDLPSIPDRPAIARVTAEGQLSLADAAQLADVSEARLQALNPGLRR
HHHHHHHCCCHHEEEECCCCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHCCHHHC
GATRPDRNAQLIVPADRRQRFLANLDTLPAEKRRTRTHYVVQSGDTLSGIAARHDIPVGV
CCCCCCCCCEEEEECHHHHHHHHHHCCCCCHHHHCCEEEEEECCCCHHHHHHHCCCCHHH
LRRQNGISGNMLRVGQSLVIPGASSRARLASREATQQVRVKPGDSLSRIAARHDVSVDDL
HCCCCCCCCCHHHCCCEEEECCCCCHHHHHHHHHHHHEECCCCCHHHHHHHHCCCCHHHH
TRWNQIQRDDYLQPGQRLTLRTPAR
HHHHHHCCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]