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The map label for this gene is gloB

Identifier: 92114067

GI number: 92114067

Start: 2199215

End: 2199982

Strand: Direct

Name: gloB

Synonym: Csal_1944

Alternate gene names: 92114067

Gene position: 2199215-2199982 (Clockwise)

Preceding gene: 92114062

Following gene: 92114068

Centisome position: 59.49

GC content: 65.89

Gene sequence:

>768_bases
ATGCTGACCGTAATACCGATCCCCGCATTTCAGGACAACTATATCTGGCTGCTTCGCCAGGATGCCAGCGATAAGGTCGT
CATCGTCGATCCCGGCGATGCCCAGCCGGTCATCGAGTACCTGGAACGAGAAGGCCTCAGCCTGGCCGCGATTCTGGTAA
CGCACCATCATCACGATCACACCGGTGGCATCGATGCGCTGGTCAAGCGGTACTCGCCCCGCGTCATCGGTCCCGACAAC
TCGGCCATTCCGGCCATCGACGAGGTCGTCGGCGACGAGGATGAATGCCGCGTGCAAGGACGGCGCTTCGAAGTCTTCGC
CGTGCCCGGCCACACGCTGGACCATATCGCGTTCTACGCCCCCGGCACACCCGGCCTGCTGTTCTGCGGCGACACCCTGT
TCTCCGGCGGCTGCGGCCGCCTCTTCGAAGGCACCGCCGAGCAGATGCACCGCTCGCTCGCGCGTCTCGCCGCCCTGCCC
GACGACACCCTGGTGTTCGCGGGACACGAATACACGCTGGCCAACCTGCGGTTCGCCCAGGCCGCCGAGCCCGACAACCC
CGCGCGGGACGCACATCTCGGCGAATGCGAGCGGGCCCGGCAGCTGGAGCGCCCCACGCTACCCAGCACCATCGGCCGGG
AGCGCCAGATCAATCCGTTTCTACGTATCGACCAGCCCGGCCTGCTGAATGCCTTGGCCGAGCAGGGAAGCGTCGATGAC
GACAGTGCCGCCTTCGCGACACTACGCGGCTGGAAGGATCGCTTCTAG

Upstream 100 bases:

>100_bases
TTGACGGATTTTACGCTGCCGCTCACGATCGAGCGCGCCCGCGAGGCGCGAGCATCGTCACCATTCGGCAGCCCCGACGT
TTGCCGAGATCCTACGCACT

Downstream 100 bases:

>100_bases
GCCATGGCCGGCTCGGCCGCTACTGTACCTCGAGAGGTTCGCTTCCCATGAAAGTACGATCGAGTCGCCGCCAGGCGCTA
CGTTTGGGTCACGTAGCCCT

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MLTVIPIPAFQDNYIWLLRQDASDKVVIVDPGDAQPVIEYLEREGLSLAAILVTHHHHDHTGGIDALVKRYSPRVIGPDN
SAIPAIDEVVGDEDECRVQGRRFEVFAVPGHTLDHIAFYAPGTPGLLFCGDTLFSGGCGRLFEGTAEQMHRSLARLAALP
DDTLVFAGHEYTLANLRFAQAAEPDNPARDAHLGECERARQLERPTLPSTIGRERQINPFLRIDQPGLLNALAEQGSVDD
DSAAFATLRGWKDRF

Sequences:

>Translated_255_residues
MLTVIPIPAFQDNYIWLLRQDASDKVVIVDPGDAQPVIEYLEREGLSLAAILVTHHHHDHTGGIDALVKRYSPRVIGPDN
SAIPAIDEVVGDEDECRVQGRRFEVFAVPGHTLDHIAFYAPGTPGLLFCGDTLFSGGCGRLFEGTAEQMHRSLARLAALP
DDTLVFAGHEYTLANLRFAQAAEPDNPARDAHLGECERARQLERPTLPSTIGRERQINPFLRIDQPGLLNALAEQGSVDD
DSAAFATLRGWKDRF
>Mature_255_residues
MLTVIPIPAFQDNYIWLLRQDASDKVVIVDPGDAQPVIEYLEREGLSLAAILVTHHHHDHTGGIDALVKRYSPRVIGPDN
SAIPAIDEVVGDEDECRVQGRRFEVFAVPGHTLDHIAFYAPGTPGLLFCGDTLFSGGCGRLFEGTAEQMHRSLARLAALP
DDTLVFAGHEYTLANLRFAQAAEPDNPARDAHLGECERARQLERPTLPSTIGRERQINPFLRIDQPGLLNALAEQGSVDD
DSAAFATLRGWKDRF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI21703352, Length=253, Percent_Identity=40.7114624505929, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI116642887, Length=253, Percent_Identity=40.7114624505929, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI94538320, Length=260, Percent_Identity=36.1538461538462, Blast_Score=162, Evalue=2e-40,
Organism=Homo sapiens, GI94538322, Length=259, Percent_Identity=35.1351351351351, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI14150041, Length=230, Percent_Identity=39.1304347826087, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI46361987, Length=209, Percent_Identity=38.755980861244, Blast_Score=133, Evalue=2e-31,
Organism=Escherichia coli, GI1786406, Length=250, Percent_Identity=46.4, Blast_Score=208, Evalue=2e-55,
Organism=Escherichia coli, GI1787158, Length=207, Percent_Identity=29.951690821256, Blast_Score=74, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17536925, Length=263, Percent_Identity=33.0798479087452, Blast_Score=154, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6320478, Length=263, Percent_Identity=30.7984790874525, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324614, Length=259, Percent_Identity=28.5714285714286, Blast_Score=98, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21356335, Length=256, Percent_Identity=38.28125, Blast_Score=183, Evalue=9e-47,
Organism=Drosophila melanogaster, GI24667711, Length=256, Percent_Identity=38.28125, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24667703, Length=256, Percent_Identity=38.28125, Blast_Score=182, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_CHRSD (Q1QW62)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_573995.1
- ProteinModelPortal:   Q1QW62
- SMR:   Q1QW62
- STRING:   Q1QW62
- GeneID:   4027184
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_1944
- NMPDR:   fig|290398.4.peg.2620
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- BioCyc:   CSAL290398:CSAL_1944-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28054; Mature: 28054

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTVIPIPAFQDNYIWLLRQDASDKVVIVDPGDAQPVIEYLEREGLSLAAILVTHHHHDH
CEEEEECCCCCCCEEEEEEECCCCCEEEECCCCCHHHHHHHHHCCCEEEEEEEEECCCCC
TGGIDALVKRYSPRVIGPDNSAIPAIDEVVGDEDECRVQGRRFEVFAVPGHTLDHIAFYA
CCCHHHHHHHCCCCEECCCCCCCCHHHHHHCCCHHHEECCCEEEEEEECCCCHHHEEEEC
PGTPGLLFCGDTLFSGGCGRLFEGTAEQMHRSLARLAALPDDTLVFAGHEYTLANLRFAQ
CCCCCEEEECHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEECEEEHH
AAEPDNPARDAHLGECERARQLERPTLPSTIGRERQINPFLRIDQPGLLNALAEQGSVDD
CCCCCCCCCCCCCHHHHHHHHHCCCCCCHHCCCCCCCCCEEEECCCHHHHHHHHCCCCCC
DSAAFATLRGWKDRF
CHHHHHHHHCCCCCC
>Mature Secondary Structure
MLTVIPIPAFQDNYIWLLRQDASDKVVIVDPGDAQPVIEYLEREGLSLAAILVTHHHHDH
CEEEEECCCCCCCEEEEEEECCCCCEEEECCCCCHHHHHHHHHCCCEEEEEEEEECCCCC
TGGIDALVKRYSPRVIGPDNSAIPAIDEVVGDEDECRVQGRRFEVFAVPGHTLDHIAFYA
CCCHHHHHHHCCCCEECCCCCCCCHHHHHHCCCHHHEECCCEEEEEEECCCCHHHEEEEC
PGTPGLLFCGDTLFSGGCGRLFEGTAEQMHRSLARLAALPDDTLVFAGHEYTLANLRFAQ
CCCCCEEEECHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEECEEEHH
AAEPDNPARDAHLGECERARQLERPTLPSTIGRERQINPFLRIDQPGLLNALAEQGSVDD
CCCCCCCCCCCCCHHHHHHHHHCCCCCCHHCCCCCCCCCEEEECCCHHHHHHHHCCCCCC
DSAAFATLRGWKDRF
CHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA