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The map label for this gene is hemK [H]

Identifier: 92113654

GI number: 92113654

Start: 1743165

End: 1744025

Strand: Direct

Name: hemK [H]

Synonym: Csal_1530

Alternate gene names: 92113654

Gene position: 1743165-1744025 (Clockwise)

Preceding gene: 92113653

Following gene: 92113655

Centisome position: 47.16

GC content: 71.08

Gene sequence:

>861_bases
ATGACCTTCGATGCCTTGCTGGCACGCGCGACGATGCGGCTGCAGGCCTCGGGGAGCCCCTCGCCGCGTCTGGACGCTGA
GGTGCTGATGATGCATGCCGCGGACGTCACGCGAGCCTGGCTGTATACCTGGGGCGACCGCGAGATTGTGCCTCCGGCAT
GGGCCCGGTTCGACGCCCTGATCGCGGCGCGGTCGCTGGGGCATCCCGTGGCGCACCTGACCGGGGAGCGTGAATTCTGG
GGGCTCGTGCTGCGTGTCGACGACACGACGCTCATTCCGCGCCCCGACACCGAATGCCTGGTCGAAGCGCTGCTGGCTCG
CATGCCGCATGCCACCGGGCGCGCGCTCGATCTGGGCACGGGATCGGGTGCCATCGCGCTGGCGCTCGCCTCGGAGCGAC
CGGCGTGGCATGTCGTCGGGGTCGACAAGGTACCCGGCGCCGTACGCCTGGCGACGGCCAATGCGGAGCGCCTGGCACTG
AGCAACGCCACCTTCCTGGTCAGCGACTGGTTCTCTGCCCTCGGCAACGAGACGTTCGACGTGATCGCGGCCAACCCGCC
CTATATCGCCGAGGACGACCCGCATCTCGCCCAGGGCGACGTGCGTTTCGAGCCCCGCTCGGCCCTGGTGGCGGAAGACG
CCGGGCTTGCCGACCTGTGTCACCTCGCGCTGACCGCGCGGGCCCATCTCGCTGGGGGCGGCTGGCTGGCCATGGAGCAC
GGCATGACGCAGGCGGCGCGGGTGCGCGAGATCCTGACGAGTGCCGGTTATCGCGATGTCGCCAGTGTCGCGGACCTCGG
GGCCCGCGAGCGCGTCACTCTGGGGCGTGCGCCCGATGCGGCGGGGCACGGCGCTCGTTGA

Upstream 100 bases:

>100_bases
ACAAGCTCGGCGACGTGATGGCGGGTGAGCTCGGCGAGGTGATCGATCCCTTGATCCACGAGTACCAGGCCGAGCAACTG
TCGGCCCTGCAGGCCTCGTG

Downstream 100 bases:

>100_bases
TTCGACGCACGTGGGGACAGACTCGATGCGCGTGGAGGAAGGCGCGGCGCGTCTGGAGGCGCGGGCGGCGAATGTGGTAG
TAATACGCCTCGCACTCGAC

Product: HemK family modification methylase

Products: NA

Alternate protein names: M.EcoKHemKP; Protein-(glutamine-N(5)) MTase hemK; Protein-glutamine N-methyltransferase hemK [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MTFDALLARATMRLQASGSPSPRLDAEVLMMHAADVTRAWLYTWGDREIVPPAWARFDALIAARSLGHPVAHLTGEREFW
GLVLRVDDTTLIPRPDTECLVEALLARMPHATGRALDLGTGSGAIALALASERPAWHVVGVDKVPGAVRLATANAERLAL
SNATFLVSDWFSALGNETFDVIAANPPYIAEDDPHLAQGDVRFEPRSALVAEDAGLADLCHLALTARAHLAGGGWLAMEH
GMTQAARVREILTSAGYRDVASVADLGARERVTLGRAPDAAGHGAR

Sequences:

>Translated_286_residues
MTFDALLARATMRLQASGSPSPRLDAEVLMMHAADVTRAWLYTWGDREIVPPAWARFDALIAARSLGHPVAHLTGEREFW
GLVLRVDDTTLIPRPDTECLVEALLARMPHATGRALDLGTGSGAIALALASERPAWHVVGVDKVPGAVRLATANAERLAL
SNATFLVSDWFSALGNETFDVIAANPPYIAEDDPHLAQGDVRFEPRSALVAEDAGLADLCHLALTARAHLAGGGWLAMEH
GMTQAARVREILTSAGYRDVASVADLGARERVTLGRAPDAAGHGAR
>Mature_285_residues
TFDALLARATMRLQASGSPSPRLDAEVLMMHAADVTRAWLYTWGDREIVPPAWARFDALIAARSLGHPVAHLTGEREFWG
LVLRVDDTTLIPRPDTECLVEALLARMPHATGRALDLGTGSGAIALALASERPAWHVVGVDKVPGAVRLATANAERLALS
NATFLVSDWFSALGNETFDVIAANPPYIAEDDPHLAQGDVRFEPRSALVAEDAGLADLCHLALTARAHLAGGGWLAMEHG
MTQAARVREILTSAGYRDVASVADLGARERVTLGRAPDAAGHGAR

Specific function: Methylates the translation termination release factor RF1/PrfA on 'Gln-235' and RF2/PrfB on 'Gln-252' [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=187, Percent_Identity=34.2245989304813, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1787463, Length=276, Percent_Identity=52.536231884058, Blast_Score=281, Evalue=4e-77,
Organism=Escherichia coli, GI87082085, Length=175, Percent_Identity=33.7142857142857, Blast_Score=77, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24582226, Length=276, Percent_Identity=25, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 30485; Mature: 30354

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFDALLARATMRLQASGSPSPRLDAEVLMMHAADVTRAWLYTWGDREIVPPAWARFDAL
CCHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHH
IAARSLGHPVAHLTGEREFWGLVLRVDDTTLIPRPDTECLVEALLARMPHATGRALDLGT
HHHHHCCCCHHHHCCCCCEEEEEEEECCCEECCCCCHHHHHHHHHHHCCCCCCCEEEECC
GSGAIALALASERPAWHVVGVDKVPGAVRLATANAERLALSNATFLVSDWFSALGNETFD
CCCEEEEEEECCCCCEEEEECCCCCCEEEEEECCCHHEEECCCEEHHHHHHHHHCCCCEE
VIAANPPYIAEDDPHLAQGDVRFEPRSALVAEDAGLADLCHLALTARAHLAGGGWLAMEH
EEECCCCCCCCCCCCCCCCCEEECCCHHEEECCCCHHHHHHHHHHHHHHCCCCCEEEECC
GMTQAARVREILTSAGYRDVASVADLGARERVTLGRAPDAAGHGAR
CCHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure 
TFDALLARATMRLQASGSPSPRLDAEVLMMHAADVTRAWLYTWGDREIVPPAWARFDAL
CHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHH
IAARSLGHPVAHLTGEREFWGLVLRVDDTTLIPRPDTECLVEALLARMPHATGRALDLGT
HHHHHCCCCHHHHCCCCCEEEEEEEECCCEECCCCCHHHHHHHHHHHCCCCCCCEEEECC
GSGAIALALASERPAWHVVGVDKVPGAVRLATANAERLALSNATFLVSDWFSALGNETFD
CCCEEEEEEECCCCCEEEEECCCCCCEEEEEECCCHHEEECCCEEHHHHHHHHHCCCCEE
VIAANPPYIAEDDPHLAQGDVRFEPRSALVAEDAGLADLCHLALTARAHLAGGGWLAMEH
EEECCCCCCCCCCCCCCCCCEEECCCHHEEECCCCHHHHHHHHHHHHHHCCCCCEEEECC
GMTQAARVREILTSAGYRDVASVADLGARERVTLGRAPDAAGHGAR
CCHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7883187; 7543480; 11805295; 8905232; 9278503 [H]