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The map label for this gene is prfA

Identifier: 92113653

GI number: 92113653

Start: 1742080

End: 1743165

Strand: Direct

Name: prfA

Synonym: Csal_1529

Alternate gene names: 92113653

Gene position: 1742080-1743165 (Clockwise)

Preceding gene: 92113652

Following gene: 92113654

Centisome position: 47.13

GC content: 65.84

Gene sequence:

>1086_bases
ATGAAAGCGACTCTGCGCACACGTCTCGATGGCTTCCAGGAACGCTTCGAAGAACTCGCCGCCTTGCTGGCCGAGCCCGA
CGTGATCGCTGATCAGGCGCGCTTTCGCGATTATTCACGCGAGTACGCCGAACTCGAGCCGCTGATCGAGGCGTGGCGCA
GCTATCGGCGCACCGAGGACGACCTCGAGGCCGCCGAGCAGATGCGCGAGGACAGTGATCCCGAGATGCGCGAGCTGGCG
GAGGAAGAATGGCGGGAGGCGCAGGCGCGTCTCGAGGCGCTGGATGCGGACGTCAAGCGCCTGCTGGTGCCCAGGGACCC
CGACGATGCCAGCAACGTCTTCCTCGAGATTCGCGCCGGCACCGGCGGTGACGAGGCGGCCCTGTTCGCCGGGGACCTGT
TTCGCATGTATTCGCGATATGCCGAAAAGGTCGGCTGGCGCATCGAGGTGATCAATGCCAGTCATGGCGAGCAGGGCGGC
TACAAGGAACTGATCGCGCGCGTACGCGGTGACAATGTCTATGCGCGTCTCAAGTTCGAGTCGGGCGCGCACCGCGTGCA
GCGGGTGCCGGCCACCGAGTCCCAGGGGCGCATTCATACCTCGGCGTGCACCGTGGCGGTGATGGCCGAGGCCTCCGCCG
TCGGTGAGGTGGAACTCAACAGCAACGATCTGCGCGTGGATACCTTTCGCTCCAGCGGGGCCGGCGGTCAGCACGTCAAT
ACCACCGATTCGGCGATTCGCATCACGCACCTGCCGAGCGGACTGGTGGTGGAGTGCCAGGAAGAACGCAGCCAGCACAA
GAACCGGGCCAAGGCGATGGCGCTGCTGGCGGCTCGCCTCAAGCAGTCCGCGGTGGACGCACAGCGCCAGCAGCAGGCCG
ATACGCGGCGCTCGCTGGTGGGCTCGGGGGACCGCAGCGAGCGCATCCGTACCTACAACTTCCCCCAGGGGCGCATTACC
GACCATCGCATCAATCTGACGCTCTACAAGCTCGGCGACGTGATGGCGGGTGAGCTCGGCGAGGTGATCGATCCCTTGAT
CCACGAGTACCAGGCCGAGCAACTGTCGGCCCTGCAGGCCTCGTGA

Upstream 100 bases:

>100_bases
CCCTGCGCTTGCGTGAAGCGTCGGGCGAGGCACGACACGATATTCTCAAGGCGGCCGATACCCTGTTGATCGACCCGGCC
GCATCGACACAGGACTCTGC

Downstream 100 bases:

>100_bases
TGACCTTCGATGCCTTGCTGGCACGCGCGACGATGCGGCTGCAGGCCTCGGGGAGCCCCTCGCCGCGTCTGGACGCTGAG
GTGCTGATGATGCATGCCGC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKATLRTRLDGFQERFEELAALLAEPDVIADQARFRDYSREYAELEPLIEAWRSYRRTEDDLEAAEQMREDSDPEMRELA
EEEWREAQARLEALDADVKRLLVPRDPDDASNVFLEIRAGTGGDEAALFAGDLFRMYSRYAEKVGWRIEVINASHGEQGG
YKELIARVRGDNVYARLKFESGAHRVQRVPATESQGRIHTSACTVAVMAEASAVGEVELNSNDLRVDTFRSSGAGGQHVN
TTDSAIRITHLPSGLVVECQEERSQHKNRAKAMALLAARLKQSAVDAQRQQQADTRRSLVGSGDRSERIRTYNFPQGRIT
DHRINLTLYKLGDVMAGELGEVIDPLIHEYQAEQLSALQAS

Sequences:

>Translated_361_residues
MKATLRTRLDGFQERFEELAALLAEPDVIADQARFRDYSREYAELEPLIEAWRSYRRTEDDLEAAEQMREDSDPEMRELA
EEEWREAQARLEALDADVKRLLVPRDPDDASNVFLEIRAGTGGDEAALFAGDLFRMYSRYAEKVGWRIEVINASHGEQGG
YKELIARVRGDNVYARLKFESGAHRVQRVPATESQGRIHTSACTVAVMAEASAVGEVELNSNDLRVDTFRSSGAGGQHVN
TTDSAIRITHLPSGLVVECQEERSQHKNRAKAMALLAARLKQSAVDAQRQQQADTRRSLVGSGDRSERIRTYNFPQGRIT
DHRINLTLYKLGDVMAGELGEVIDPLIHEYQAEQLSALQAS
>Mature_361_residues
MKATLRTRLDGFQERFEELAALLAEPDVIADQARFRDYSREYAELEPLIEAWRSYRRTEDDLEAAEQMREDSDPEMRELA
EEEWREAQARLEALDADVKRLLVPRDPDDASNVFLEIRAGTGGDEAALFAGDLFRMYSRYAEKVGWRIEVINASHGEQGG
YKELIARVRGDNVYARLKFESGAHRVQRVPATESQGRIHTSACTVAVMAEASAVGEVELNSNDLRVDTFRSSGAGGQHVN
TTDSAIRITHLPSGLVVECQEERSQHKNRAKAMALLAARLKQSAVDAQRQQQADTRRSLVGSGDRSERIRTYNFPQGRIT
DHRINLTLYKLGDVMAGELGEVIDPLIHEYQAEQLSALQAS

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=290, Percent_Identity=45.8620689655172, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI34577120, Length=352, Percent_Identity=38.6363636363636, Blast_Score=245, Evalue=5e-65,
Organism=Homo sapiens, GI166795305, Length=195, Percent_Identity=45.1282051282051, Blast_Score=178, Evalue=8e-45,
Organism=Escherichia coli, GI1787462, Length=358, Percent_Identity=62.2905027932961, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI2367172, Length=341, Percent_Identity=36.0703812316716, Blast_Score=202, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17542784, Length=289, Percent_Identity=36.6782006920415, Blast_Score=184, Evalue=7e-47,
Organism=Saccharomyces cerevisiae, GI6321295, Length=316, Percent_Identity=41.7721518987342, Blast_Score=241, Evalue=1e-64,
Organism=Drosophila melanogaster, GI19921226, Length=327, Percent_Identity=41.2844036697248, Blast_Score=228, Evalue=7e-60,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_CHRSD (Q1QXC6)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_573581.1
- ProteinModelPortal:   Q1QXC6
- SMR:   Q1QXC6
- STRING:   Q1QXC6
- GeneID:   4029231
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_1529
- NMPDR:   fig|290398.4.peg.2479
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- BioCyc:   CSAL290398:CSAL_1529-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40562; Mature: 40562

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKATLRTRLDGFQERFEELAALLAEPDVIADQARFRDYSREYAELEPLIEAWRSYRRTED
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
DLEAAEQMREDSDPEMRELAEEEWREAQARLEALDADVKRLLVPRDPDDASNVFLEIRAG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
TGGDEAALFAGDLFRMYSRYAEKVGWRIEVINASHGEQGGYKELIARVRGDNVYARLKFE
CCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEEC
SGAHRVQRVPATESQGRIHTSACTVAVMAEASAVGEVELNSNDLRVDTFRSSGAGGQHVN
CCCHHHHCCCCCCCCCCEEHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCCCCCC
TTDSAIRITHLPSGLVVECQEERSQHKNRAKAMALLAARLKQSAVDAQRQQQADTRRSLV
CCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSGDRSERIRTYNFPQGRITDHRINLTLYKLGDVMAGELGEVIDPLIHEYQAEQLSALQA
CCCCCCCCCEEECCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
S
C
>Mature Secondary Structure
MKATLRTRLDGFQERFEELAALLAEPDVIADQARFRDYSREYAELEPLIEAWRSYRRTED
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
DLEAAEQMREDSDPEMRELAEEEWREAQARLEALDADVKRLLVPRDPDDASNVFLEIRAG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
TGGDEAALFAGDLFRMYSRYAEKVGWRIEVINASHGEQGGYKELIARVRGDNVYARLKFE
CCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEEC
SGAHRVQRVPATESQGRIHTSACTVAVMAEASAVGEVELNSNDLRVDTFRSSGAGGQHVN
CCCHHHHCCCCCCCCCCEEHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCCCCCC
TTDSAIRITHLPSGLVVECQEERSQHKNRAKAMALLAARLKQSAVDAQRQQQADTRRSLV
CCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSGDRSERIRTYNFPQGRITDHRINLTLYKLGDVMAGELGEVIDPLIHEYQAEQLSALQA
CCCCCCCCCEEECCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA