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The map label for this gene is degA [H]

Identifier: 92112209

GI number: 92112209

Start: 92956

End: 94011

Strand: Direct

Name: degA [H]

Synonym: Csal_0074

Alternate gene names: 92112209

Gene position: 92956-94011 (Clockwise)

Preceding gene: 92112204

Following gene: 92112213

Centisome position: 2.51

GC content: 65.91

Gene sequence:

>1056_bases
ATGACGCGTCGACCGACCCTCAAGACTATCGCTGATCGGGTGGGCGTTACCGTCTCCGCGGTATCGCTGGCCCTGCGCGA
CGATCCACGTATCTCCGCGACGACGCGTTCACGTATCCAGGAGGCCGCCGAGGCACTGGGTTACGTCTACAATCGACATG
CGGCGGGATTGCGACGTGGCGACAGCGGCACCGTGGCGGTCTGCCTCAACGACCTGAGCAACCCTTTCTTCACCGAATTC
CTCGGCCATATGGAGACCCGTTTCCGCGAGGCGGGACGCATGATGCTGTTCTGTCATGCCCATGAGACACCCTCGATCCA
GGCTCGCTTCATCCGCCAGATGGCCGAACACGGTGCCGCCGGATTGATTCTGGTCCCGGTGGAGGGCACGTCGCGCGCGG
ACCTGGAAGGCCCCCGCGTGCGTCATCTGCGCGATTTTCCACTGGTGCTGATTTCGCGCGATGTCGCCGATACGGCGTTC
GACCGGGTGATCAACGACGATGAACGCGGCATCCGGCTATTGTTCGAGCACCTGTATGCCCTGGGGCACCGCCGCCTGGC
CTGGCTGGGCGGAGGGGGCGATACCTCCACGGCGCACGATCGCGAGCGCTCCTTTCGCCGCGAGATGGACGCCGCAGGAT
TGCCGATCGATGACGCGACGATGCACCACGGCCCGACGTCATTGGCCTTTGGCGATAGCATGCTCGACGAGTTGATGGCG
CTGCCGACACCGCCTACGGCGATCGTGTGTTTTTCCGACGTGATCGCGTTCGGGGTTCTGGCTGCCTGCTATCGGCGGGG
GCTGCGCCCGGGCATCGACCTTTCGGTGACCGGCTTTGACGACATGGGCGCCGCTGCCTATTCGGCGCCGGCTCTGACCA
GTGTCCGCGTGCGCACCGACTTGATCGGCGATCGCGCCAGCGAGTTGCTGCTGGCCCGCATCGCCGGCGACCGTCAGCCG
GCGGTGCGGGAAATGATGCCGCCCGAACTGGTGGTCCGCGAGACCACCGGTCCTCCGCCCGAGACAGGCATGCTACCGCC
ACGGCGGCGTGGGTGA

Upstream 100 bases:

>100_bases
AGGCCGCTGGTGAGGAGATTGCTCGCGGCGCCACGACCATCACGCCGACAGGCTTCGACAGCGGGCCCTCGCCAGGGCCC
CGCCGGAACAGGACTCTTGC

Downstream 100 bases:

>100_bases
GTGGTGCTGGGCGGGTAAAAACGACGCGAGGCCGGCATCGGCCTCGCGTCGTTCCCTGGTGGCGATGGGGTGTCGTCAGG
TCACGCCCGTTTCATGGCGC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Degradation activator [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MTRRPTLKTIADRVGVTVSAVSLALRDDPRISATTRSRIQEAAEALGYVYNRHAAGLRRGDSGTVAVCLNDLSNPFFTEF
LGHMETRFREAGRMMLFCHAHETPSIQARFIRQMAEHGAAGLILVPVEGTSRADLEGPRVRHLRDFPLVLISRDVADTAF
DRVINDDERGIRLLFEHLYALGHRRLAWLGGGGDTSTAHDRERSFRREMDAAGLPIDDATMHHGPTSLAFGDSMLDELMA
LPTPPTAIVCFSDVIAFGVLAACYRRGLRPGIDLSVTGFDDMGAAAYSAPALTSVRVRTDLIGDRASELLLARIAGDRQP
AVREMMPPELVVRETTGPPPETGMLPPRRRG

Sequences:

>Translated_351_residues
MTRRPTLKTIADRVGVTVSAVSLALRDDPRISATTRSRIQEAAEALGYVYNRHAAGLRRGDSGTVAVCLNDLSNPFFTEF
LGHMETRFREAGRMMLFCHAHETPSIQARFIRQMAEHGAAGLILVPVEGTSRADLEGPRVRHLRDFPLVLISRDVADTAF
DRVINDDERGIRLLFEHLYALGHRRLAWLGGGGDTSTAHDRERSFRREMDAAGLPIDDATMHHGPTSLAFGDSMLDELMA
LPTPPTAIVCFSDVIAFGVLAACYRRGLRPGIDLSVTGFDDMGAAAYSAPALTSVRVRTDLIGDRASELLLARIAGDRQP
AVREMMPPELVVRETTGPPPETGMLPPRRRG
>Mature_350_residues
TRRPTLKTIADRVGVTVSAVSLALRDDPRISATTRSRIQEAAEALGYVYNRHAAGLRRGDSGTVAVCLNDLSNPFFTEFL
GHMETRFREAGRMMLFCHAHETPSIQARFIRQMAEHGAAGLILVPVEGTSRADLEGPRVRHLRDFPLVLISRDVADTAFD
RVINDDERGIRLLFEHLYALGHRRLAWLGGGGDTSTAHDRERSFRREMDAAGLPIDDATMHHGPTSLAFGDSMLDELMAL
PTPPTAIVCFSDVIAFGVLAACYRRGLRPGIDLSVTGFDDMGAAAYSAPALTSVRVRTDLIGDRASELLLARIAGDRQPA
VREMMPPELVVRETTGPPPETGMLPPRRRG

Specific function: Involved in the control of degradation of B.subtilis amidophosphoribosyltransferase (purF). Probably activates the gene for a degradative protease [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=332, Percent_Identity=26.8072289156627, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787906, Length=269, Percent_Identity=29.7397769516729, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1789202, Length=335, Percent_Identity=27.1641791044776, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787948, Length=336, Percent_Identity=24.4047619047619, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790194, Length=319, Percent_Identity=26.0188087774295, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI48994940, Length=316, Percent_Identity=27.8481012658228, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1788474, Length=333, Percent_Identity=25.2252252252252, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786540, Length=349, Percent_Identity=26.3610315186246, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789068, Length=318, Percent_Identity=23.8993710691824, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1789456, Length=344, Percent_Identity=25, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1790689, Length=326, Percent_Identity=25.7668711656442, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787580, Length=324, Percent_Identity=22.2222222222222, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786268, Length=319, Percent_Identity=21.3166144200627, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38447; Mature: 38316

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRPTLKTIADRVGVTVSAVSLALRDDPRISATTRSRIQEAAEALGYVYNRHAAGLRRG
CCCCCHHHHHHHHHCCHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DSGTVAVCLNDLSNPFFTEFLGHMETRFREAGRMMLFCHAHETPSIQARFIRQMAEHGAA
CCCEEEEEEHHCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCC
GLILVPVEGTSRADLEGPRVRHLRDFPLVLISRDVADTAFDRVINDDERGIRLLFEHLYA
CEEEEECCCCCCCCCCCCHHHHHCCCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHH
LGHRRLAWLGGGGDTSTAHDRERSFRREMDAAGLPIDDATMHHGPTSLAFGDSMLDELMA
HCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LPTPPTAIVCFSDVIAFGVLAACYRRGLRPGIDLSVTGFDDMGAAAYSAPALTSVRVRTD
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHCCCCHHEEHHHHH
LIGDRASELLLARIAGDRQPAVREMMPPELVVRETTGPPPETGMLPPRRRG
HHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRRPTLKTIADRVGVTVSAVSLALRDDPRISATTRSRIQEAAEALGYVYNRHAAGLRRG
CCCCHHHHHHHHHCCHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DSGTVAVCLNDLSNPFFTEFLGHMETRFREAGRMMLFCHAHETPSIQARFIRQMAEHGAA
CCCEEEEEEHHCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCC
GLILVPVEGTSRADLEGPRVRHLRDFPLVLISRDVADTAFDRVINDDERGIRLLFEHLYA
CEEEEECCCCCCCCCCCCHHHHHCCCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHH
LGHRRLAWLGGGGDTSTAHDRERSFRREMDAAGLPIDDATMHHGPTSLAFGDSMLDELMA
HCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LPTPPTAIVCFSDVIAFGVLAACYRRGLRPGIDLSVTGFDDMGAAAYSAPALTSVRVRTD
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHCCCCHHEEHHHHH
LIGDRASELLLARIAGDRQPAVREMMPPELVVRETTGPPPETGMLPPRRRG
HHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407808; 9353932; 9384377 [H]