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The map label for this gene is trmE

Identifier: 91785808

GI number: 91785808

Start: 4889839

End: 4891233

Strand: Reverse

Name: trmE

Synonym: Bxe_A4469

Alternate gene names: 91785808

Gene position: 4891233-4889839 (Counterclockwise)

Preceding gene: 91785811

Following gene: 91785807

Centisome position: 99.91

GC content: 66.95

Gene sequence:

>1395_bases
ATGCTCACCACCGATTCCGATCCCATCGTCGCCATCGCCACCGCGCCCGGCCGGGGAGGTATCGGCGTGGTGCGGATTTC
GTTCGGCCGAGCGGGTGAGGCGGCGGCCCAGCCGCTGATGCAAGCGCTCACCGGTCAGACACTCGCCGCCCGCCGCGCCA
GCTACGTTCCGTTCCTCGACGCCAGCGGCGATGCGCTCGACCGTGGCATCGCGCTGTACTTCCCGGCGCCACACTCCTAT
ACGGGCGAACACGTGCTCGAACTGCAGGGCCACGGCGGTCCGGTCGTGCTGCAACTCGTGCTGCAGCGTTGCATCGACGC
CGGCCGCGCCTTCGGTCTACGTCTGGCCGAACCGGGCGAGTTCACGCGCCGCGCCTTCCTCAACGACAAGCTGGATCTGG
CGCAAGCCGAAGCGGTCGCCGATCTGATCGAGGCCAGCACCGAGGCCGCCGCGCGCTCGGCGGGCCGCTCGCTCGACGGC
GCATTTTCGCGCGACATTCACGCGTTGGTCGAAGACGTGATCACGCTGCGCATGCTCGTCGAAGCGACGCTCGACTTTCC
CGAGGAGGAGATCGACTTCCTCGAAGCCGCCGACGCCCGCGGCAAGCTCGCGCGGATTCGCGAGCGCCTCGGGCACGTGC
TGAGCGAGGCGCGGCAAGGCGCGTTGTTGCGCGAGGGCCTCTCGGTGGTGCTGGCCGGGCAGCCCAACGTCGGCAAATCG
TCGCTGCTGAATGCGCTGGCGGGCGCGGAACTGGCGATCGTCACGCCGATCGCCGGCACCACGCGGGACAAGGTCGCGCA
GACGATCCAGATCGAAGGCATTCCGCTACACGTGATCGATACCGCCGGCCTGCGCGATACCGAGGACGAAGTCGAAAAAA
TCGGCATTGCGCGCACCTGGAACGAGATCGAACGCGCGGATGTCGTACTGCATCTGCTCGATGCGCGCACCGGCATGACG
GTCGAAGACGAGGCGATCGCCGGGCGCTTTCCGGCCGGCGTGCCGGTGGTGCGCGTGCTGAACAAGACGGACCTGACCGG
CCTTGCGCCGGCAACGCGAGCACTCGACGCAGACCTGGATCTGAGCGAAGTGCGGCTCTCGGCGAAACAGGGCGACGGCG
TAGCGCTGTTGCGCGAAGAGTTGCTGCGGATCGCCGGCTGGCAAGCAGGCGCGGAAAGCGTGTACCTGGCGCGCGAACGG
CATCTGATTGCACTACGCGCCGCCGAAGAACATCTCGCCACCGCAGCCGCGCACGCCGATCAGAACTCCCAGGCGCTGGA
CCTATTCGCTGAAGAGCTTCGGCTCGCGCAGGACCAGCTGAATTCCATCACGGGCGAATTCAGTTCGGATGACCTGCTCG
GAGTGATTTTCAGCAGGTTCTGTATCGGCAAGTGA

Upstream 100 bases:

>100_bases
CGCGCCGCCTGTCAGCCTTCACACACCGCCGCGTTACAATGCCTCGCGCCGCGTTGACCGCCAGCACCCTGCCCTCCTCA
CCGTTTTTGCCCACGCTTCC

Downstream 100 bases:

>100_bases
ACGAGGCTCGGCCTCCACCTCATAAAGGTTATATTTCAGGCTTTCACGAGGGATTCGGCGACCGTCCTCATCCATATGAA
ATCTCGCTTCGTTGACCCAA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MLTTDSDPIVAIATAPGRGGIGVVRISFGRAGEAAAQPLMQALTGQTLAARRASYVPFLDASGDALDRGIALYFPAPHSY
TGEHVLELQGHGGPVVLQLVLQRCIDAGRAFGLRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDG
AFSRDIHALVEDVITLRMLVEATLDFPEEEIDFLEAADARGKLARIRERLGHVLSEARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQIEGIPLHVIDTAGLRDTEDEVEKIGIARTWNEIERADVVLHLLDARTGMT
VEDEAIAGRFPAGVPVVRVLNKTDLTGLAPATRALDADLDLSEVRLSAKQGDGVALLREELLRIAGWQAGAESVYLARER
HLIALRAAEEHLATAAAHADQNSQALDLFAEELRLAQDQLNSITGEFSSDDLLGVIFSRFCIGK

Sequences:

>Translated_464_residues
MLTTDSDPIVAIATAPGRGGIGVVRISFGRAGEAAAQPLMQALTGQTLAARRASYVPFLDASGDALDRGIALYFPAPHSY
TGEHVLELQGHGGPVVLQLVLQRCIDAGRAFGLRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDG
AFSRDIHALVEDVITLRMLVEATLDFPEEEIDFLEAADARGKLARIRERLGHVLSEARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQIEGIPLHVIDTAGLRDTEDEVEKIGIARTWNEIERADVVLHLLDARTGMT
VEDEAIAGRFPAGVPVVRVLNKTDLTGLAPATRALDADLDLSEVRLSAKQGDGVALLREELLRIAGWQAGAESVYLARER
HLIALRAAEEHLATAAAHADQNSQALDLFAEELRLAQDQLNSITGEFSSDDLLGVIFSRFCIGK
>Mature_464_residues
MLTTDSDPIVAIATAPGRGGIGVVRISFGRAGEAAAQPLMQALTGQTLAARRASYVPFLDASGDALDRGIALYFPAPHSY
TGEHVLELQGHGGPVVLQLVLQRCIDAGRAFGLRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDG
AFSRDIHALVEDVITLRMLVEATLDFPEEEIDFLEAADARGKLARIRERLGHVLSEARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQIEGIPLHVIDTAGLRDTEDEVEKIGIARTWNEIERADVVLHLLDARTGMT
VEDEAIAGRFPAGVPVVRVLNKTDLTGLAPATRALDADLDLSEVRLSAKQGDGVALLREELLRIAGWQAGAESVYLARER
HLIALRAAEEHLATAAAHADQNSQALDLFAEELRLAQDQLNSITGEFSSDDLLGVIFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=472, Percent_Identity=37.2881355932203, Blast_Score=225, Evalue=6e-59,
Organism=Homo sapiens, GI193082993, Length=462, Percent_Identity=36.7965367965368, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI193082988, Length=502, Percent_Identity=34.8605577689243, Blast_Score=213, Evalue=3e-55,
Organism=Escherichia coli, GI2367268, Length=471, Percent_Identity=54.7770700636943, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI87082120, Length=157, Percent_Identity=42.0382165605096, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17507259, Length=446, Percent_Identity=28.47533632287, Blast_Score=144, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6323665, Length=493, Percent_Identity=29.2089249492901, Blast_Score=191, Evalue=2e-49,
Organism=Drosophila melanogaster, GI45550813, Length=482, Percent_Identity=33.8174273858921, Blast_Score=191, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_BURXL (Q13SH7)

Other databases:

- EMBL:   CP000270
- RefSeq:   YP_561014.1
- ProteinModelPortal:   Q13SH7
- SMR:   Q13SH7
- GeneID:   4006463
- GenomeReviews:   CP000270_GR
- KEGG:   bxe:Bxe_A4469
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 49462; Mature: 49462

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: BINDING 25-25 BINDING 87-87 BINDING 130-130 BINDING 464-464

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTTDSDPIVAIATAPGRGGIGVVRISFGRAGEAAAQPLMQALTGQTLAARRASYVPFLD
CCCCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHCCCCCEEC
ASGDALDRGIALYFPAPHSYTGEHVLELQGHGGPVVLQLVLQRCIDAGRAFGLRLAEPGE
CCCCHHHCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHCCEEECCCCH
FTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDGAFSRDIHALVEDVITLRMLV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
EATLDFPEEEIDFLEAADARGKLARIRERLGHVLSEARQGALLREGLSVVLAGQPNVGKS
HHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHH
SLLNALAGAELAIVTPIAGTTRDKVAQTIQIEGIPLHVIDTAGLRDTEDEVEKIGIARTW
HHHHHHCCCCEEEEECCCCCCHHHHHHEEEECCCEEEEEECCCCCCCHHHHHHHCHHHHH
NEIERADVVLHLLDARTGMTVEDEAIAGRFPAGVPVVRVLNKTDLTGLAPATRALDADLD
HHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHHHHHCCCCCCCCCCHHHHHCCCCC
LSEVRLSAKQGDGVALLREELLRIAGWQAGAESVYLARERHLIALRAAEEHLATAAAHAD
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEHHHHHHHHHHHHCC
QNSQALDLFAEELRLAQDQLNSITGEFSSDDLLGVIFSRFCIGK
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTTDSDPIVAIATAPGRGGIGVVRISFGRAGEAAAQPLMQALTGQTLAARRASYVPFLD
CCCCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHCCCCCEEC
ASGDALDRGIALYFPAPHSYTGEHVLELQGHGGPVVLQLVLQRCIDAGRAFGLRLAEPGE
CCCCHHHCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHCCEEECCCCH
FTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDGAFSRDIHALVEDVITLRMLV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
EATLDFPEEEIDFLEAADARGKLARIRERLGHVLSEARQGALLREGLSVVLAGQPNVGKS
HHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHH
SLLNALAGAELAIVTPIAGTTRDKVAQTIQIEGIPLHVIDTAGLRDTEDEVEKIGIARTW
HHHHHHCCCCEEEEECCCCCCHHHHHHEEEECCCEEEEEECCCCCCCHHHHHHHCHHHHH
NEIERADVVLHLLDARTGMTVEDEAIAGRFPAGVPVVRVLNKTDLTGLAPATRALDADLD
HHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHHHHHCCCCCCCCCCHHHHHCCCCC
LSEVRLSAKQGDGVALLREELLRIAGWQAGAESVYLARERHLIALRAAEEHLATAAAHAD
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEHHHHHHHHHHHHCC
QNSQALDLFAEELRLAQDQLNSITGEFSSDDLLGVIFSRFCIGK
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA