| Definition | Burkholderia xenovorans LB400 chromosome 1, complete sequence. |
|---|---|
| Accession | NC_007951 |
| Length | 4,895,836 |
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The map label for this gene is murE [H]
Identifier: 91785298
GI number: 91785298
Start: 4348368
End: 4349924
Strand: Reverse
Name: murE [H]
Synonym: Bxe_A0481
Alternate gene names: 91785298
Gene position: 4349924-4348368 (Counterclockwise)
Preceding gene: 91785299
Following gene: 91785297
Centisome position: 88.85
GC content: 67.31
Gene sequence:
>1557_bases ATGAGTGCGCTGCGTCAGAAGCATCCCGCGCAGCGGCAGATCGCCGACGCCCTCACCTGGCTGCACGCTCACGTGCAGCC AGGTGCGCATCTGCACGCCGACACGCGCTCGCTCGCGGCGGGCGACGCGTTTTTCGCCTACGCGGTGGACGGCGCGGACA ACCGCCCGTTCATCGAGGGTGCGATCGAGCGTGGCGCCGCCGCCGTGCTGGTTCAGCCCGAGGGCTTCAGCGGCGCGATC AATCCGTCGAACATGCTCGCCGTGCCGGCGTTGAACGAACTCGCGGGTTCGATCGCGAGCGGCTGGTACAACGACCCGAG CGACAGCATGCTCACGGTCGGCATTACCGGCACGAACGGCAAGACTTCGTGCAGCCAGTGGATCTCGGCGGCGCTGACGG CGCTCGGCACGCGTTGCGCGATCATCGGCACGCTCGGCACGGGTTTGCCGGGTCACCTGGTGCACACGGGTTTCACCACC CCCGACGCGCCGCAACTGCAACGCAGCCTTGCGCAATTGCGCGACGCGGGTGCGCAGGCGGTAGCGATGGAAGTGTCGTC GCATGCGCTGCACCAGGGCCGCGTGAACGGCACGGCGTTCGACATCGCCGTATTCACGAATCTCACGCAGGACCATCTCG ACTATCACCAGACATTCGAAGCCTACGAAGCCGCCAAAGCGCGCCTCTTCGCCTGGCCGGAACTGCGTGCGGCGGTGATC AATCGCGACGATGCGGCCGGCCGCCGTTTGCTCGCGAGCACGCAAGGCCACGCGCGCACGATCGCGTACGGGCTCGACAG CGGGCAAGGCGCGCTGCAGGACGCACCGCAAGCCGACGCGTTGCTGCTCGCCTCGAATGTGCGCGCCACGGCCACCGGTA CCGCGTTCCATCTGAGCACCTCGGACTGGGGCGATGCCGAAGTCGAAGTGCAAACGCTCGGCGCGTTCAACGTGAGCAAT CTGCTCGGCGTGCTGGGGGCATTGCTCGCCGCCGACGTGCCGTTCGAAGCCGCGCTGGCCGAACTGGCGCAGCTCGAACC GGTGAACGGCCGCATGCAGCGTCTGGGCGGCCGCTTGCAGAACGACGAACCGCTCGTCGTGATCGATTACGCGCACACGC CGGATGCGCTGGAAAAAACACTCGAAGCGCTGCGCCCGATGGTAACCGCGCGCGGCGGCGAACTGATCTGCATGTTCGGC TGCGGCGGCGACCGCGACGCCACCAAGCGTCCGCTGATGGGCGCGATTGCCGAGAAGATCGCCGACGGCGTGGTGGTGAC CAGCGACAACCCGCGCAGCGAAGATCCGCAATCGATCATCGAGCAGATCGCGGCGGGTATGAAAGACGCGTCGAAGGCGC GCCGTATCGAGGACCGCGCAAGTGCGATCCTGCAGGCGATCCGCAGCGCCGCGCGCGAAGACGTCATCGTGCTGGCCGGC AAGGGGCATGAAGCAACACAGGAAATCATGGGTAAGAAACGCGCTTTCTCCGATCAGGATCACGCGCGCCTCGCGCTGGC CGCACGCGCGACGCACGCACGCGGAGGTGGCGAATGA
Upstream 100 bases:
>100_bases CGCCCGCCACGCCTGCCACGGTCAAGAAACTCTCCACCAGCGCCGGCGCGAAGAAGATGACGATTGCCGCGAACGCGAGA AGCCATCCGGGAGTCGTGCG
Downstream 100 bases:
>100_bases GCATGTTCTCGCTGCGTGAAGCCGCGGCGCTGATCCCGGGCGCAACCGTGCTCGGCGACGACAGCGTCACTATTGAACGC ATCTCGACCGACAGCCGCAG
Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase
Products: NA
Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]
Number of amino acids: Translated: 518; Mature: 517
Protein sequence:
>518_residues MSALRQKHPAQRQIADALTWLHAHVQPGAHLHADTRSLAAGDAFFAYAVDGADNRPFIEGAIERGAAAVLVQPEGFSGAI NPSNMLAVPALNELAGSIASGWYNDPSDSMLTVGITGTNGKTSCSQWISAALTALGTRCAIIGTLGTGLPGHLVHTGFTT PDAPQLQRSLAQLRDAGAQAVAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHQTFEAYEAAKARLFAWPELRAAVI NRDDAAGRRLLASTQGHARTIAYGLDSGQGALQDAPQADALLLASNVRATATGTAFHLSTSDWGDAEVEVQTLGAFNVSN LLGVLGALLAADVPFEAALAELAQLEPVNGRMQRLGGRLQNDEPLVVIDYAHTPDALEKTLEALRPMVTARGGELICMFG CGGDRDATKRPLMGAIAEKIADGVVVTSDNPRSEDPQSIIEQIAAGMKDASKARRIEDRASAILQAIRSAAREDVIVLAG KGHEATQEIMGKKRAFSDQDHARLALAARATHARGGGE
Sequences:
>Translated_518_residues MSALRQKHPAQRQIADALTWLHAHVQPGAHLHADTRSLAAGDAFFAYAVDGADNRPFIEGAIERGAAAVLVQPEGFSGAI NPSNMLAVPALNELAGSIASGWYNDPSDSMLTVGITGTNGKTSCSQWISAALTALGTRCAIIGTLGTGLPGHLVHTGFTT PDAPQLQRSLAQLRDAGAQAVAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHQTFEAYEAAKARLFAWPELRAAVI NRDDAAGRRLLASTQGHARTIAYGLDSGQGALQDAPQADALLLASNVRATATGTAFHLSTSDWGDAEVEVQTLGAFNVSN LLGVLGALLAADVPFEAALAELAQLEPVNGRMQRLGGRLQNDEPLVVIDYAHTPDALEKTLEALRPMVTARGGELICMFG CGGDRDATKRPLMGAIAEKIADGVVVTSDNPRSEDPQSIIEQIAAGMKDASKARRIEDRASAILQAIRSAAREDVIVLAG KGHEATQEIMGKKRAFSDQDHARLALAARATHARGGGE >Mature_517_residues SALRQKHPAQRQIADALTWLHAHVQPGAHLHADTRSLAAGDAFFAYAVDGADNRPFIEGAIERGAAAVLVQPEGFSGAIN PSNMLAVPALNELAGSIASGWYNDPSDSMLTVGITGTNGKTSCSQWISAALTALGTRCAIIGTLGTGLPGHLVHTGFTTP DAPQLQRSLAQLRDAGAQAVAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHQTFEAYEAAKARLFAWPELRAAVIN RDDAAGRRLLASTQGHARTIAYGLDSGQGALQDAPQADALLLASNVRATATGTAFHLSTSDWGDAEVEVQTLGAFNVSNL LGVLGALLAADVPFEAALAELAQLEPVNGRMQRLGGRLQNDEPLVVIDYAHTPDALEKTLEALRPMVTARGGELICMFGC GGDRDATKRPLMGAIAEKIADGVVVTSDNPRSEDPQSIIEQIAAGMKDASKARRIEDRASAILQAIRSAAREDVIVLAGK GHEATQEIMGKKRAFSDQDHARLALAARATHARGGGE
Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]
COG id: COG0769
COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the MurCDEF family. MurE subfamily [H]
Homologues:
Organism=Escherichia coli, GI1786273, Length=474, Percent_Identity=43.8818565400844, Blast_Score=308, Evalue=4e-85,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004101 - InterPro: IPR013221 - InterPro: IPR005761 [H]
Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]
EC number: =6.3.2.13 [H]
Molecular weight: Translated: 54518; Mature: 54387
Theoretical pI: Translated: 5.94; Mature: 5.94
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 1.9 %Met (Translated Protein) 2.7 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 1.7 %Met (Mature Protein) 2.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSALRQKHPAQRQIADALTWLHAHVQPGAHLHADTRSLAAGDAFFAYAVDGADNRPFIEG CCCHHHCCCHHHHHHHHHHHHHHHCCCCCEEECCCHHHHCCCEEEEEEECCCCCCCHHHH AIERGAAAVLVQPEGFSGAINPSNMLAVPALNELAGSIASGWYNDPSDSMLTVGITGTNG HHCCCCEEEEECCCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCC KTSCSQWISAALTALGTRCAIIGTLGTGLPGHLVHTGFTTPDAPQLQRSLAQLRDAGAQA CHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCE VAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHQTFEAYEAAKARLFAWPELRAAVI EEHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHEEECHHHHHHHH NRDDAAGRRLLASTQGHARTIAYGLDSGQGALQDAPQADALLLASNVRATATGTAFHLST CCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCEEEECCCEEEEEC SDWGDAEVEVQTLGAFNVSNLLGVLGALLAADVPFEAALAELAQLEPVNGRMQRLGGRLQ CCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHCCCCC NDEPLVVIDYAHTPDALEKTLEALRPMVTARGGELICMFGCGGDRDATKRPLMGAIAEKI CCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHH ADGVVVTSDNPRSEDPQSIIEQIAAGMKDASKARRIEDRASAILQAIRSAAREDVIVLAG HCCEEEECCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC KGHEATQEIMGKKRAFSDQDHARLALAARATHARGGGE CCCHHHHHHHHHHHCCCCCCHHHEEHHHHHHCCCCCCC >Mature Secondary Structure SALRQKHPAQRQIADALTWLHAHVQPGAHLHADTRSLAAGDAFFAYAVDGADNRPFIEG CCHHHCCCHHHHHHHHHHHHHHHCCCCCEEECCCHHHHCCCEEEEEEECCCCCCCHHHH AIERGAAAVLVQPEGFSGAINPSNMLAVPALNELAGSIASGWYNDPSDSMLTVGITGTNG HHCCCCEEEEECCCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCC KTSCSQWISAALTALGTRCAIIGTLGTGLPGHLVHTGFTTPDAPQLQRSLAQLRDAGAQA CHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCE VAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHQTFEAYEAAKARLFAWPELRAAVI EEHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHEEECHHHHHHHH NRDDAAGRRLLASTQGHARTIAYGLDSGQGALQDAPQADALLLASNVRATATGTAFHLST CCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCEEEECCCEEEEEC SDWGDAEVEVQTLGAFNVSNLLGVLGALLAADVPFEAALAELAQLEPVNGRMQRLGGRLQ CCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHCCCCC NDEPLVVIDYAHTPDALEKTLEALRPMVTARGGELICMFGCGGDRDATKRPLMGAIAEKI CCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHH ADGVVVTSDNPRSEDPQSIIEQIAAGMKDASKARRIEDRASAILQAIRSAAREDVIVLAG HCCEEEECCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC KGHEATQEIMGKKRAFSDQDHARLALAARATHARGGGE CCCHHHHHHHHHHHCCCCCCHHHEEHHHHHHCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11823852 [H]