| Definition | Burkholderia xenovorans LB400 chromosome 1, complete sequence. |
|---|---|
| Accession | NC_007951 |
| Length | 4,895,836 |
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The map label for this gene is murF [H]
Identifier: 91785297
GI number: 91785297
Start: 4346959
End: 4348371
Strand: Reverse
Name: murF [H]
Synonym: Bxe_A0482
Alternate gene names: 91785297
Gene position: 4348371-4346959 (Counterclockwise)
Preceding gene: 91785298
Following gene: 91785296
Centisome position: 88.82
GC content: 66.81
Gene sequence:
>1413_bases ATGAGCATGTTCTCGCTGCGTGAAGCCGCGGCGCTGATCCCGGGCGCAACCGTGCTCGGCGACGACAGCGTCACTATTGA ACGCATCTCGACCGACAGCCGCAGCGCCGGCCCGGGCGATCTGTTTGTCGCGATCAAGGGCGACCGGTTCGACGCGCACG ACTTTCTGCCGGACGTGGCCGTGCGCAACGTGAGCGCTGTTCTGGTTACGCGCACGCCGCAAGACTGGAACGTACCGGCG CTCAAAGTCGCCGACACGCGCGTCGGCCTGGGTGCGCTCGCACGCGGCTGGCGCCGCCGGTTCAGCATGCCGCTCGTCGC CGTGACGGGCAGCAACGGCAAGACCACGGTCAAGGAAATGATCGCGTCGATCTTCGCGGCTGCGGTCGGCGCCGACGCAC GTCTCGCCACTGCGGGCAACTTCAACAACGACATCGGCTTGCCGCTCACGTTGTTCCGGCTGCACGCGGCGCACCAACTG GCGGTGGTCGAACTCGGCATGAACCATCCGGGCGAAACCTCCGAGCTCGCGAAAATCGCCGAGCCGACGGTTGCCGTCGT GAACAACGCACAGCGTGAGCACCAGGAATTCATGGCGACGGTCGAGGCTGTCGCGCTGGAACACGCGAGCGTGATTCATG CGCTGTCGCCCGAAGGCGTCGCGGTGTTCCCCGCCGACGACGCTTACGCGAGCATCTGGCGCGTGGCCGCAACCGGCAAC CGCATCATGGACTTCGCGCTGAACTCCGCGGAGCGCACCACTGAAGCTGCAGTAAAAGGCACGTTCGCTGGCAACGTCCT GAGCATCGACACGCCGGCAGGCCGGCTCGAAGTCACGCTGCAGGTGCTCGGCGACCACAACGCGCACAACGCACTGGCCG CAACGTCCGCCGCTTTGGCCGCGGGCGTTTCGCTCGACGCGATCAGGCGCGGTCTCGAATCGTTCGGCGCCGTAAAGGGC CGCTTGCAGGTGAAGCAAGGCGTCCTCGGCTCGCTCGCCGGTGCAACCGTGATCGACGACACCTACAACGCCAATCCCGA TTCGATGCGCGCCGCAATCGACGTGCTCGCCTCGCGCCCCGCACCCCGCGTGCTGGTGATGGGCGACATGGGCGAAGTCG GCGACAACGGTCCGGCGTTCCACCGCGAAATCGGCGCATACGCCAGGGACCGCGGTATCGACGCGCTATACGCAATGGGT GACGCCTCGCGCGACGCCTGCACCGCGTATGGCGCGAACGCGCACCACGTCGCCGACATCGGCACGCTGGTCGCGCAATT GCAGCAGGCCGGTTTCGGACCTGCCGCAACGCTTCTCGTGAAAGGCTCGCGCTTCATGCAGATGGAGCGCGTGGTGGACG CCGTTACGAGTCCACAACCCAACGCAGCGGGCAGCACGCCCGCCGCACATTGA
Upstream 100 bases:
>100_bases CAACACAGGAAATCATGGGTAAGAAACGCGCTTTCTCCGATCAGGATCACGCGCGCCTCGCGCTGGCCGCACGCGCGACG CACGCACGCGGAGGTGGCGA
Downstream 100 bases:
>100_bases AATAGAAGGACCGAAGCATGCTACTGGCGCTGGCGCAATGGCTGCAGAATGACGCAAGCTTCTTGCGCGTGTTCAGTTAT CTGACTTTCCGTGCGGTGAT
Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase
Products: NA
Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]
Number of amino acids: Translated: 470; Mature: 469
Protein sequence:
>470_residues MSMFSLREAAALIPGATVLGDDSVTIERISTDSRSAGPGDLFVAIKGDRFDAHDFLPDVAVRNVSAVLVTRTPQDWNVPA LKVADTRVGLGALARGWRRRFSMPLVAVTGSNGKTTVKEMIASIFAAAVGADARLATAGNFNNDIGLPLTLFRLHAAHQL AVVELGMNHPGETSELAKIAEPTVAVVNNAQREHQEFMATVEAVALEHASVIHALSPEGVAVFPADDAYASIWRVAATGN RIMDFALNSAERTTEAAVKGTFAGNVLSIDTPAGRLEVTLQVLGDHNAHNALAATSAALAAGVSLDAIRRGLESFGAVKG RLQVKQGVLGSLAGATVIDDTYNANPDSMRAAIDVLASRPAPRVLVMGDMGEVGDNGPAFHREIGAYARDRGIDALYAMG DASRDACTAYGANAHHVADIGTLVAQLQQAGFGPAATLLVKGSRFMQMERVVDAVTSPQPNAAGSTPAAH
Sequences:
>Translated_470_residues MSMFSLREAAALIPGATVLGDDSVTIERISTDSRSAGPGDLFVAIKGDRFDAHDFLPDVAVRNVSAVLVTRTPQDWNVPA LKVADTRVGLGALARGWRRRFSMPLVAVTGSNGKTTVKEMIASIFAAAVGADARLATAGNFNNDIGLPLTLFRLHAAHQL AVVELGMNHPGETSELAKIAEPTVAVVNNAQREHQEFMATVEAVALEHASVIHALSPEGVAVFPADDAYASIWRVAATGN RIMDFALNSAERTTEAAVKGTFAGNVLSIDTPAGRLEVTLQVLGDHNAHNALAATSAALAAGVSLDAIRRGLESFGAVKG RLQVKQGVLGSLAGATVIDDTYNANPDSMRAAIDVLASRPAPRVLVMGDMGEVGDNGPAFHREIGAYARDRGIDALYAMG DASRDACTAYGANAHHVADIGTLVAQLQQAGFGPAATLLVKGSRFMQMERVVDAVTSPQPNAAGSTPAAH >Mature_469_residues SMFSLREAAALIPGATVLGDDSVTIERISTDSRSAGPGDLFVAIKGDRFDAHDFLPDVAVRNVSAVLVTRTPQDWNVPAL KVADTRVGLGALARGWRRRFSMPLVAVTGSNGKTTVKEMIASIFAAAVGADARLATAGNFNNDIGLPLTLFRLHAAHQLA VVELGMNHPGETSELAKIAEPTVAVVNNAQREHQEFMATVEAVALEHASVIHALSPEGVAVFPADDAYASIWRVAATGNR IMDFALNSAERTTEAAVKGTFAGNVLSIDTPAGRLEVTLQVLGDHNAHNALAATSAALAAGVSLDAIRRGLESFGAVKGR LQVKQGVLGSLAGATVIDDTYNANPDSMRAAIDVLASRPAPRVLVMGDMGEVGDNGPAFHREIGAYARDRGIDALYAMGD ASRDACTAYGANAHHVADIGTLVAQLQQAGFGPAATLLVKGSRFMQMERVVDAVTSPQPNAAGSTPAAH
Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]
COG id: COG0770
COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase
Gene ontology:
Cell location: Cytoplasm (Probable) [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the MurCDEF family [H]
Homologues:
Organism=Escherichia coli, GI1786274, Length=434, Percent_Identity=39.8617511520737, Blast_Score=258, Evalue=5e-70,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004101 - InterPro: IPR013221 - InterPro: IPR000713 - InterPro: IPR005863 [H]
Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]
EC number: =6.3.2.10 [H]
Molecular weight: Translated: 48977; Mature: 48846
Theoretical pI: Translated: 6.05; Mature: 6.05
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 2.8 %Met (Translated Protein) 3.0 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 2.6 %Met (Mature Protein) 2.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSMFSLREAAALIPGATVLGDDSVTIERISTDSRSAGPGDLFVAIKGDRFDAHDFLPDVA CCCHHHHHHHHHCCCCEEECCCCEEEEEECCCCCCCCCCEEEEEEECCCCCHHHHCHHHH VRNVSAVLVTRTPQDWNVPALKVADTRVGLGALARGWRRRFSMPLVAVTGSNGKTTVKEM HCCCEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHH IASIFAAAVGADARLATAGNFNNDIGLPLTLFRLHAAHQLAVVELGMNHPGETSELAKIA HHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHC EPTVAVVNNAQREHQEFMATVEAVALEHASVIHALSPEGVAVFPADDAYASIWRVAATGN CCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCHHHHHHHHHCCCC RIMDFALNSAERTTEAAVKGTFAGNVLSIDTPAGRLEVTLQVLGDHNAHNALAATSAALA CEEHHHHCCHHHHHHHHHHEEECCCEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHH AGVSLDAIRRGLESFGAVKGRLQVKQGVLGSLAGATVIDDTYNANPDSMRAAIDVLASRP HCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCEEECCCCCCCHHHHHHHHHHHHCCC APRVLVMGDMGEVGDNGPAFHREIGAYARDRGIDALYAMGDASRDACTAYGANAHHVADI CCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHCCCCCHHHHHH GTLVAQLQQAGFGPAATLLVKGSRFMQMERVVDAVTSPQPNAAGSTPAAH HHHHHHHHHCCCCCHHEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCC >Mature Secondary Structure SMFSLREAAALIPGATVLGDDSVTIERISTDSRSAGPGDLFVAIKGDRFDAHDFLPDVA CCHHHHHHHHHCCCCEEECCCCEEEEEECCCCCCCCCCEEEEEEECCCCCHHHHCHHHH VRNVSAVLVTRTPQDWNVPALKVADTRVGLGALARGWRRRFSMPLVAVTGSNGKTTVKEM HCCCEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHH IASIFAAAVGADARLATAGNFNNDIGLPLTLFRLHAAHQLAVVELGMNHPGETSELAKIA HHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHC EPTVAVVNNAQREHQEFMATVEAVALEHASVIHALSPEGVAVFPADDAYASIWRVAATGN CCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCHHHHHHHHHCCCC RIMDFALNSAERTTEAAVKGTFAGNVLSIDTPAGRLEVTLQVLGDHNAHNALAATSAALA CEEHHHHCCHHHHHHHHHHEEECCCEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHH AGVSLDAIRRGLESFGAVKGRLQVKQGVLGSLAGATVIDDTYNANPDSMRAAIDVLASRP HCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCEEECCCCCCCHHHHHHHHHHHHCCC APRVLVMGDMGEVGDNGPAFHREIGAYARDRGIDALYAMGDASRDACTAYGANAHHVADI CCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHCCCCCHHHHHH GTLVAQLQQAGFGPAATLLVKGSRFMQMERVVDAVTSPQPNAAGSTPAAH HHHHHHHHHCCCCCHHEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 7542800 [H]