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The map label for this gene is penA [H]

Identifier: 91785299

GI number: 91785299

Start: 4349921

End: 4351786

Strand: Reverse

Name: penA [H]

Synonym: Bxe_A0480

Alternate gene names: 91785299

Gene position: 4351786-4349921 (Counterclockwise)

Preceding gene: 91785300

Following gene: 91785298

Centisome position: 88.89

GC content: 64.58

Gene sequence:

>1866_bases
ATGAAAAAATCGTCGGCTCGCAAGAGCGTGGCTTTCTCGGCCAACCCGATCCTGTCGGTGCGCCTGCCAATGTGGCGCTC
CAAGCTCGTCGTGTTCATGCTTTTCATGGCGTTCGTCGCGCTCACCGCGCGCGCCTTCTGGATTCAGGGCCCGGGCAACG
CGTTCTATCAGAAGCAGGGTGAAATCCGCTATCAGCGCCGCCTCGAACTGCCGGCTACGCGCGGCAAGATTCTCGACCGC
AACGGTCTCGTGCTGGCCACGAGTCTGCCGGTGCGCGCGATCTGGGCGATTCCCGAATCGGTGCCGGACGACCTCGGCGC
CGACAAGCTGACTTCGCTCGGCAAGCTGCTCGGCATGACGAACAAGGAACTGCGCACCAAGTTGTCGGAAGACAAAAACT
TCGTCTACGTGAAACGCCAGGTGCCGGTGGACATTGCGGAAAAAGTCACCGCGCTGGAGATTCCCGGCATTTACTCGCGC
GACGAGTACAAGCGCTTCTATCCGGAAGGCGAGATCACGGCCCACCTGATCGGCTTTACCAACGTGGAAGACGAAGGCCA
GGAAGGCGTCGAACTCGGCGACCAGAAGCTGCTCGCCGGCGTGTCGGGCAGCCGCCGCGTGATCAAGGACCGCATGGGCC
ACATCATCGAGGACGTGGACGAGCAGGTCGTGCCGCACAACGGCCAGGACGTGGATCTCTCGATCGACAGCAAGATTCAG
TACATCGCTTATACGAACCTGAAGGCGGCCGTCGAAAAATTCAAGGCGAAGGCCGGCGCGGCAATGGTGATCGACGTGCG
CACCGGCGAAGTACTGGCGCTCGTCAATTACCCCACGTACAACCCGAACGACCGCACTCACCTGACCGGCGACCAGTTGC
GCAACCGCATCCTGACCGACACGTTCGAACCCGGCTCGATCATGAAGCCGTTCACGGTCTCGCTCGCGCTCGATCTGCAC
CGTGTCACGCCGACTACACTGGTTGATACAGGCGGCGGCCGTTTCGTGCTCGACGGCGCGCCGATTACCGACGACAGCGC
GTTCGGCGTGCTGACCGTGGGCGGCGTGATCCAGAAGTCGAGCAACATCGGCGCGACGAAGATCGCCATGCAGCTCAAGC
CCGAAGAGATGTGGAACATGTACACCAGCATCGGTCTCGGCCAGGCGCCGAAGGTCGGCTTCCCGGGCGCGGCGGCTGGC
CGTCTGCGTCCGTGGAAGAGCTGGCGCCGCATCGAGCAGGCCACCATGTCGTACGGCTATGGTCTGTCGGTGTCGCTGTT
CCAGTTGGGCCGCGCTTACACCGCGATCGCGCATGACGGCGAGATCATGCCGGTGTCGATCTTCCGCACGCCTGGCGATC
AGCCGGCCACCGGCCCGCAGATCTTCGCGCCCACTACCGCTCGCGAAGTGCGCGCCATGCTCGAAACCGTCACGGCGCCG
GGCGGCACCTCGCCGGATGCGGCGGTGCCGGGCTATCGCGTCGGCGGCAAGAGCGGCACGGCGTACAAGCACGGCGCGCA
CGGCTACGACCACTCCAAATATCGCGCGTCGTTCGTCGGCATGGCGCCCATGCCGAATCCGCGCATCGTCGTGGCCGTTT
CGGTCGACGAACCGACCGCGGGCAGCCACTTCGGTGGTCAGGTGTCGGGCCCGGTGTTCTCCAGCATCGTCGGCGATACG
CTGCGCTCGCTGAACGTGCCGCCGGACATGCCGGTCAAGCAGATGGTGGTGTCAGACGACTCGGCCCCGCCGGCGCCGAC
CGCGCCCGCCACGCCTGCCACGGTCAAGAAACTCTCCACCAGCGCCGGCGCGAAGAAGATGACGATTGCCGCGAACGCGA
GAAGCCATCCGGGAGTCGTGCGATGA

Upstream 100 bases:

>100_bases
ACCTCACGCTCGATCCGGGCGCGGTGAAAGCTGAAGACGCGCCGATCCCGACTTCCGGCCCCGCATCGGCGCCGGCGGCG
ACCCGTCGCGGAGGCGTGCG

Downstream 100 bases:

>100_bases
GTGCGCTGCGTCAGAAGCATCCCGCGCAGCGGCAGATCGCCGACGCCCTCACCTGGCTGCACGCTCACGTGCAGCCAGGT
GCGCATCTGCACGCCGACAC

Product: peptidoglycan synthetase FtsI

Products: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-; $diphosphoundecaprenol; undecaprenyl diphosphate

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 621; Mature: 621

Protein sequence:

>621_residues
MKKSSARKSVAFSANPILSVRLPMWRSKLVVFMLFMAFVALTARAFWIQGPGNAFYQKQGEIRYQRRLELPATRGKILDR
NGLVLATSLPVRAIWAIPESVPDDLGADKLTSLGKLLGMTNKELRTKLSEDKNFVYVKRQVPVDIAEKVTALEIPGIYSR
DEYKRFYPEGEITAHLIGFTNVEDEGQEGVELGDQKLLAGVSGSRRVIKDRMGHIIEDVDEQVVPHNGQDVDLSIDSKIQ
YIAYTNLKAAVEKFKAKAGAAMVIDVRTGEVLALVNYPTYNPNDRTHLTGDQLRNRILTDTFEPGSIMKPFTVSLALDLH
RVTPTTLVDTGGGRFVLDGAPITDDSAFGVLTVGGVIQKSSNIGATKIAMQLKPEEMWNMYTSIGLGQAPKVGFPGAAAG
RLRPWKSWRRIEQATMSYGYGLSVSLFQLGRAYTAIAHDGEIMPVSIFRTPGDQPATGPQIFAPTTAREVRAMLETVTAP
GGTSPDAAVPGYRVGGKSGTAYKHGAHGYDHSKYRASFVGMAPMPNPRIVVAVSVDEPTAGSHFGGQVSGPVFSSIVGDT
LRSLNVPPDMPVKQMVVSDDSAPPAPTAPATPATVKKLSTSAGAKKMTIAANARSHPGVVR

Sequences:

>Translated_621_residues
MKKSSARKSVAFSANPILSVRLPMWRSKLVVFMLFMAFVALTARAFWIQGPGNAFYQKQGEIRYQRRLELPATRGKILDR
NGLVLATSLPVRAIWAIPESVPDDLGADKLTSLGKLLGMTNKELRTKLSEDKNFVYVKRQVPVDIAEKVTALEIPGIYSR
DEYKRFYPEGEITAHLIGFTNVEDEGQEGVELGDQKLLAGVSGSRRVIKDRMGHIIEDVDEQVVPHNGQDVDLSIDSKIQ
YIAYTNLKAAVEKFKAKAGAAMVIDVRTGEVLALVNYPTYNPNDRTHLTGDQLRNRILTDTFEPGSIMKPFTVSLALDLH
RVTPTTLVDTGGGRFVLDGAPITDDSAFGVLTVGGVIQKSSNIGATKIAMQLKPEEMWNMYTSIGLGQAPKVGFPGAAAG
RLRPWKSWRRIEQATMSYGYGLSVSLFQLGRAYTAIAHDGEIMPVSIFRTPGDQPATGPQIFAPTTAREVRAMLETVTAP
GGTSPDAAVPGYRVGGKSGTAYKHGAHGYDHSKYRASFVGMAPMPNPRIVVAVSVDEPTAGSHFGGQVSGPVFSSIVGDT
LRSLNVPPDMPVKQMVVSDDSAPPAPTAPATPATVKKLSTSAGAKKMTIAANARSHPGVVR
>Mature_621_residues
MKKSSARKSVAFSANPILSVRLPMWRSKLVVFMLFMAFVALTARAFWIQGPGNAFYQKQGEIRYQRRLELPATRGKILDR
NGLVLATSLPVRAIWAIPESVPDDLGADKLTSLGKLLGMTNKELRTKLSEDKNFVYVKRQVPVDIAEKVTALEIPGIYSR
DEYKRFYPEGEITAHLIGFTNVEDEGQEGVELGDQKLLAGVSGSRRVIKDRMGHIIEDVDEQVVPHNGQDVDLSIDSKIQ
YIAYTNLKAAVEKFKAKAGAAMVIDVRTGEVLALVNYPTYNPNDRTHLTGDQLRNRILTDTFEPGSIMKPFTVSLALDLH
RVTPTTLVDTGGGRFVLDGAPITDDSAFGVLTVGGVIQKSSNIGATKIAMQLKPEEMWNMYTSIGLGQAPKVGFPGAAAG
RLRPWKSWRRIEQATMSYGYGLSVSLFQLGRAYTAIAHDGEIMPVSIFRTPGDQPATGPQIFAPTTAREVRAMLETVTAP
GGTSPDAAVPGYRVGGKSGTAYKHGAHGYDHSKYRASFVGMAPMPNPRIVVAVSVDEPTAGSHFGGQVSGPVFSSIVGDT
LRSLNVPPDMPVKQMVVSDDSAPPAPTAPATPATVKKLSTSAGAKKMTIAANARSHPGVVR

Specific function: Synthesis of cross-linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=553, Percent_Identity=40.3254972875226, Blast_Score=398, Evalue=1e-112,
Organism=Escherichia coli, GI1786854, Length=623, Percent_Identity=23.916532905297, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: 2.4.1.129

Molecular weight: Translated: 67060; Mature: 67060

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSSARKSVAFSANPILSVRLPMWRSKLVVFMLFMAFVALTARAFWIQGPGNAFYQKQG
CCCCCCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHCCCC
EIRYQRRLELPATRGKILDRNGLVLATSLPVRAIWAIPESVPDDLGADKLTSLGKLLGMT
CEEEEEEECCCCCCCCEECCCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCC
NKELRTKLSEDKNFVYVKRQVPVDIAEKVTALEIPGIYSRDEYKRFYPEGEITAHLIGFT
CHHHHHHHCCCCCEEEEEEECCCCHHHCEEEEECCCCCCCCHHHHHCCCCCEEEEEEEEC
NVEDEGQEGVELGDQKLLAGVSGSRRVIKDRMGHIIEDVDEQVVPHNGQDVDLSIDSKIQ
CCCCCCCCCHHCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEE
YIAYTNLKAAVEKFKAKAGAAMVIDVRTGEVLALVNYPTYNPNDRTHLTGDQLRNRILTD
EEEECCHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCEECHHHHHHHHHCC
TFEPGSIMKPFTVSLALDLHRVTPTTLVDTGGGRFVLDGAPITDDSAFGVLTVGGVIQKS
CCCCCCCCCCEEEEEEEEEECCCCCEEEECCCCEEEECCCCCCCCCCCEEEEECCCCCCC
SNIGATKIAMQLKPEEMWNMYTSIGLGQAPKVGFPGAAAGRLRPWKSWRRIEQATMSYGY
CCCCEEEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
GLSVSLFQLGRAYTAIAHDGEIMPVSIFRTPGDQPATGPQIFAPTTAREVRAMLETVTAP
CCEEHHHHHCCCEEEEECCCCEEEEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHCCC
GGTSPDAAVPGYRVGGKSGTAYKHGAHGYDHSKYRASFVGMAPMPNPRIVVAVSVDEPTA
CCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHEEEEEECCCCCCCCEEEEEEECCCCC
GSHFGGQVSGPVFSSIVGDTLRSLNVPPDMPVKQMVVSDDSAPPAPTAPATPATVKKLST
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHEECCCCCCCCCCCCCCCHHHHHHHH
SAGAKKMTIAANARSHPGVVR
CCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MKKSSARKSVAFSANPILSVRLPMWRSKLVVFMLFMAFVALTARAFWIQGPGNAFYQKQG
CCCCCCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHCCCC
EIRYQRRLELPATRGKILDRNGLVLATSLPVRAIWAIPESVPDDLGADKLTSLGKLLGMT
CEEEEEEECCCCCCCCEECCCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCC
NKELRTKLSEDKNFVYVKRQVPVDIAEKVTALEIPGIYSRDEYKRFYPEGEITAHLIGFT
CHHHHHHHCCCCCEEEEEEECCCCHHHCEEEEECCCCCCCCHHHHHCCCCCEEEEEEEEC
NVEDEGQEGVELGDQKLLAGVSGSRRVIKDRMGHIIEDVDEQVVPHNGQDVDLSIDSKIQ
CCCCCCCCCHHCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEE
YIAYTNLKAAVEKFKAKAGAAMVIDVRTGEVLALVNYPTYNPNDRTHLTGDQLRNRILTD
EEEECCHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCEECHHHHHHHHHCC
TFEPGSIMKPFTVSLALDLHRVTPTTLVDTGGGRFVLDGAPITDDSAFGVLTVGGVIQKS
CCCCCCCCCCEEEEEEEEEECCCCCEEEECCCCEEEECCCCCCCCCCCEEEEECCCCCCC
SNIGATKIAMQLKPEEMWNMYTSIGLGQAPKVGFPGAAAGRLRPWKSWRRIEQATMSYGY
CCCCEEEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
GLSVSLFQLGRAYTAIAHDGEIMPVSIFRTPGDQPATGPQIFAPTTAREVRAMLETVTAP
CCEEHHHHHCCCEEEEECCCCEEEEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHCCC
GGTSPDAAVPGYRVGGKSGTAYKHGAHGYDHSKYRASFVGMAPMPNPRIVVAVSVDEPTA
CCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHEEEEEECCCCCCCCEEEEEEECCCCC
GSHFGGQVSGPVFSSIVGDTLRSLNVPPDMPVKQMVVSDDSAPPAPTAPATPATVKKLST
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHEECCCCCCCCCCCCCCCHHHHHHHH
SAGAKKMTIAANARSHPGVVR
CCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-; $diphosphoundecaprenol; GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-; $diphosphoundecaprenol

Specific reaction: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n- $diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu- $L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = [GlcNAc-(1->4)- $Mur2Ac(oyl-L-Ala-gamma-

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3126399 [H]