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The map label for this gene is dctA2 [H]

Identifier: 91785211

GI number: 91785211

Start: 4257686

End: 4259008

Strand: Reverse

Name: dctA2 [H]

Synonym: Bxe_A0568

Alternate gene names: 91785211

Gene position: 4259008-4257686 (Counterclockwise)

Preceding gene: 91785213

Following gene: 91785210

Centisome position: 86.99

GC content: 62.89

Gene sequence:

>1323_bases
GTGTTGAAATTTTTCAATTCGCTGTTTGGCCGGGTCGTCATAGCGCTGGTGGCGGGTATCGTGATCGGCGCCGTGTACCC
GCATTTCGCCCAATCGCTGCGCCCGCTCGGCGACGGCTTTCTCAAACTGATCAAGATGGTGATCGGCCCGATCGTGTTCT
GCGTCGTGGTGAGCGGCATGGCGCATGCCGGCGACCTGCGCAAGGTGGGGCGCGTCGGCCTGAAGGCCGTGATCTACTTC
GAGGTGATGACGACGATCGCGCTCGTGATCGGCGCAATACTCGCGTATGTCACGCAGCCCGGCGTCGGCATGAACATCGA
TCTGCGCTCGCTGGATCCTTCGTCGCTCTCGACTTACACCGAAAACGCGAAGAGCCTGAAGGACACGGCAGGCTTCCTGC
TGAAGATCATCCCCGACACGGCGATCAACGCGTTCGCTACCGGCGACATCCTGCAGATTCTGGTGTTTTCGGTGCTGTTC
GGCTCGGCACTCTCGCTGCTCGGCGGCAAGGCGCAGCGTGTGAGCAGCCTGATCGACGAACTGTCGCAGGTGTTTTTCCG
CGTAATGGGCTTCATCATCAAGCTCGCGCCGCTCGGCGTGCTCGGGGCGATTGCCTTCACCACCGGCACCTATGGCGTCG
AGTCGCTCAAGCAGCTCGGCATGCTGGTGCTCGTGTTCTACGTGAGCTGCTTCGTGTTCGTGGCTGTGGTGCTGGGCGTC
GTGATGCGGCTCGCCGGCTTCAGCATCTTCAAGCTGATCCGCTACCTGCGCGAAGAACTGTCGATCGTGCTCGGCACCGC
CTCGTCCGATGCCGTGCTGCCGCAGATCATGCGCAAGCTCGAATGGATGGGCGTCAAGGACTCCACCGTCGGGCTCGTCA
TTCCGACCGGCTACTCGTTCAATCTCGACGGCTTCTCCATCTATCTCACGCTGGCGGTGATTTTCATCGCGCAGGCCACC
AATACGCCGCTGTCCGTGCATGACCTGATCGTGGTGGTGCTGGTTTCGCTGGTGACGTCGAAGGGCGCGCACGGCATTCC
CGGCTCGGCCATCGTGATTCTGGCCGCGACGTTGTCCGCGATTCCGGCGATTCCCGTGCTGGGTCTCGTGCTGATCCTGC
CGGTCGACTGGTTCGTCGGCATTGCGCGTGCGCTGACCAACCTGATCGGCAACTGCGTGGCGACGGTGGTGGTCGCCGTG
TGGGAAAACGATATCGACCGGGCGCGCGCGCATCGCGTGCTGAATCGCGACGAAGCGTTGCGCTACGTGCCGGCCGGTGA
AGACGCCGAGCCTGCCGCGGGCGAACACGCTCCGGCCGTCTGA

Upstream 100 bases:

>100_bases
CATGAACCCTTGACGCCAGTATATCGGCTTCCTACTATTCGACATAACAGCACTGGTCGGACCAGTCTGAACAGATCTGA
ATCGCAACCAGGAGGAAGCC

Downstream 100 bases:

>100_bases
ACCTGAGAAACCGTATCCAGATCGGCGCGCCAACGCAGCGCGCCTCCAGCGCCGGCAGATCCCCAGGAACGCCGGCTCTC
CTTCACTTTTTCGCCGCCTG

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MLKFFNSLFGRVVIALVAGIVIGAVYPHFAQSLRPLGDGFLKLIKMVIGPIVFCVVVSGMAHAGDLRKVGRVGLKAVIYF
EVMTTIALVIGAILAYVTQPGVGMNIDLRSLDPSSLSTYTENAKSLKDTAGFLLKIIPDTAINAFATGDILQILVFSVLF
GSALSLLGGKAQRVSSLIDELSQVFFRVMGFIIKLAPLGVLGAIAFTTGTYGVESLKQLGMLVLVFYVSCFVFVAVVLGV
VMRLAGFSIFKLIRYLREELSIVLGTASSDAVLPQIMRKLEWMGVKDSTVGLVIPTGYSFNLDGFSIYLTLAVIFIAQAT
NTPLSVHDLIVVVLVSLVTSKGAHGIPGSAIVILAATLSAIPAIPVLGLVLILPVDWFVGIARALTNLIGNCVATVVVAV
WENDIDRARAHRVLNRDEALRYVPAGEDAEPAAGEHAPAV

Sequences:

>Translated_440_residues
MLKFFNSLFGRVVIALVAGIVIGAVYPHFAQSLRPLGDGFLKLIKMVIGPIVFCVVVSGMAHAGDLRKVGRVGLKAVIYF
EVMTTIALVIGAILAYVTQPGVGMNIDLRSLDPSSLSTYTENAKSLKDTAGFLLKIIPDTAINAFATGDILQILVFSVLF
GSALSLLGGKAQRVSSLIDELSQVFFRVMGFIIKLAPLGVLGAIAFTTGTYGVESLKQLGMLVLVFYVSCFVFVAVVLGV
VMRLAGFSIFKLIRYLREELSIVLGTASSDAVLPQIMRKLEWMGVKDSTVGLVIPTGYSFNLDGFSIYLTLAVIFIAQAT
NTPLSVHDLIVVVLVSLVTSKGAHGIPGSAIVILAATLSAIPAIPVLGLVLILPVDWFVGIARALTNLIGNCVATVVVAV
WENDIDRARAHRVLNRDEALRYVPAGEDAEPAAGEHAPAV
>Mature_440_residues
MLKFFNSLFGRVVIALVAGIVIGAVYPHFAQSLRPLGDGFLKLIKMVIGPIVFCVVVSGMAHAGDLRKVGRVGLKAVIYF
EVMTTIALVIGAILAYVTQPGVGMNIDLRSLDPSSLSTYTENAKSLKDTAGFLLKIIPDTAINAFATGDILQILVFSVLF
GSALSLLGGKAQRVSSLIDELSQVFFRVMGFIIKLAPLGVLGAIAFTTGTYGVESLKQLGMLVLVFYVSCFVFVAVVLGV
VMRLAGFSIFKLIRYLREELSIVLGTASSDAVLPQIMRKLEWMGVKDSTVGLVIPTGYSFNLDGFSIYLTLAVIFIAQAT
NTPLSVHDLIVVVLVSLVTSKGAHGIPGSAIVILAATLSAIPAIPVLGLVLILPVDWFVGIARALTNLIGNCVATVVVAV
WENDIDRARAHRVLNRDEALRYVPAGEDAEPAAGEHAPAV

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI4827012, Length=437, Percent_Identity=27.4599542334096, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI169790839, Length=472, Percent_Identity=25.4237288135593, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI21314632, Length=450, Percent_Identity=26.2222222222222, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI40254478, Length=474, Percent_Identity=24.6835443037975, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI66773030, Length=393, Percent_Identity=24.6819338422392, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI5032093, Length=405, Percent_Identity=25.4320987654321, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI223468566, Length=272, Percent_Identity=26.4705882352941, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI223468564, Length=272, Percent_Identity=26.4705882352941, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI194239697, Length=444, Percent_Identity=24.0990990990991, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI262359914, Length=387, Percent_Identity=24.8062015503876, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI301601644, Length=214, Percent_Identity=27.5700934579439, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1789947, Length=426, Percent_Identity=50.4694835680751, Blast_Score=434, Evalue=1e-123,
Organism=Escherichia coli, GI1790514, Length=423, Percent_Identity=39.9527186761229, Blast_Score=288, Evalue=5e-79,
Organism=Escherichia coli, GI1788024, Length=419, Percent_Identity=22.9116945107399, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1789473, Length=330, Percent_Identity=22.4242424242424, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193206654, Length=420, Percent_Identity=25, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17537407, Length=466, Percent_Identity=26.1802575107296, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71996953, Length=448, Percent_Identity=25, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71983106, Length=433, Percent_Identity=24.9422632794457, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71983099, Length=433, Percent_Identity=24.9422632794457, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI193206505, Length=416, Percent_Identity=25.7211538461538, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17541374, Length=402, Percent_Identity=25.3731343283582, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24583025, Length=419, Percent_Identity=24.8210023866348, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17137668, Length=419, Percent_Identity=24.8210023866348, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24583023, Length=419, Percent_Identity=24.8210023866348, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281360483, Length=450, Percent_Identity=25.3333333333333, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI17137666, Length=450, Percent_Identity=25.3333333333333, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI281360481, Length=450, Percent_Identity=25.3333333333333, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 46755; Mature: 46755

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure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EECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]