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The map label for this gene is rpoS [H]

Identifier: 91783466

GI number: 91783466

Start: 2309767

End: 2310849

Strand: Direct

Name: rpoS [H]

Synonym: Bxe_A2350

Alternate gene names: 91783466

Gene position: 2309767-2310849 (Clockwise)

Preceding gene: 91783465

Following gene: 91783467

Centisome position: 47.18

GC content: 63.07

Gene sequence:

>1083_bases
ATGCCGAAATCGAAGCGCCCGCCACCGCAAGCCGAGTCTGAGACGATCAGCCACGCTATGTCCGCTTCGGTGGATGAAAG
CGGCGCTTCGGAAGTGGAAGAGGAGATCGCAGAGGAGCGCGATCTCGACGAACGCCAGGGCGGCGCGGACGAAGCGGGCG
ACGCCCGCGAAGGCGCGAGCGAAGTCGCGCCCGACGCGGACGATTTCCGCGCGCTGCTGCAAGCCGAGCTGACGGCCGAC
ACGATTCAGCACTATCTGAACCGTATCAGCGTCAAGCCGCTGCTGACCGTCGAGGAAGAGCAGAAGTATTCGCGTCTCGC
CAAGGCGGGCGAGTTCGAAGCGCGGCAGGTCATGATCGAGCGCAATTTAAGGCTCGTTGTCAGCATTGCGAAGGGTTATC
TGAACCGCGGCGTGCCGCTGCTCGATCTGATCGAAGAAGGCAATCTCGGCCTGATGCACGCAATCGAGAAATTCGACCCG
ACGCGCGGCTTCCGCTTCTCCACCTACGCCACCTGGTGGATCCGCCAGAGTATCGAGCGCGCGATCATGAACCAGGCGCG
CACCGTCCGTTTGCCGGTGCACGTGATTCGTGAGCTCAATCAGGTGCTGCGCGCCAAGCGCCATCTGGAAAAGAATTCGA
TGAACTCCGGCGAAGCCGCCGAGCGCCGCGACGCCAGCATCGACGACATTGCCTATCTGACCGGCAAGACCACCGACGAA
GTGACGGACATCCTCGCGCTGAACGAGCACACCGCGTCGCTCGACGCGCCGCTCGATCTCGATCCGGCGAGCAGCCTGCT
CGACCTGCTGTCGGACGACCAGAGCCAGTCGCCGGACGCCGAAGTGCAGCACCGCGAACTGGAAACGCTCACGCGCGCGT
GGCTTGCGCGTTTGTCGGACAAGCACCGTCACGTGATCGAGCGCCGCTTCGGCCTGAATCACATCGAACCCGCCACGCTC
GAAGAACTGGCCGACGAAATGGGCCTCACGCGCGAGCGTGTGCGCCAGATCCAACAGGAAGCGCTCGTGCGGTTAAAGCG
CTTCTTCGCCTCCAACGGTGTTCGGAAAGACGCCGTTCTATAA

Upstream 100 bases:

>100_bases
ATAGCGATTCCGACCGCGTGATGTTGCATTTCGAAGTTCGCCGGCAGGGTAAACCTGTCGACCCACTGAAGTATTTGCCG
CCGCAATAAGCGATACGACC

Downstream 100 bases:

>100_bases
CCTGATGACACCGATTCTTGTTTTCGACATCGAGACGATTCCCGATGTCGCCGGCATTCGCCGCCTCGAAGATCTGCCCG
CCACGCTCAGCGACGCCGAA

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MPKSKRPPPQAESETISHAMSASVDESGASEVEEEIAEERDLDERQGGADEAGDAREGASEVAPDADDFRALLQAELTAD
TIQHYLNRISVKPLLTVEEEQKYSRLAKAGEFEARQVMIERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEKFDP
TRGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKNSMNSGEAAERRDASIDDIAYLTGKTTDE
VTDILALNEHTASLDAPLDLDPASSLLDLLSDDQSQSPDAEVQHRELETLTRAWLARLSDKHRHVIERRFGLNHIEPATL
EELADEMGLTRERVRQIQQEALVRLKRFFASNGVRKDAVL

Sequences:

>Translated_360_residues
MPKSKRPPPQAESETISHAMSASVDESGASEVEEEIAEERDLDERQGGADEAGDAREGASEVAPDADDFRALLQAELTAD
TIQHYLNRISVKPLLTVEEEQKYSRLAKAGEFEARQVMIERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEKFDP
TRGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKNSMNSGEAAERRDASIDDIAYLTGKTTDE
VTDILALNEHTASLDAPLDLDPASSLLDLLSDDQSQSPDAEVQHRELETLTRAWLARLSDKHRHVIERRFGLNHIEPATL
EELADEMGLTRERVRQIQQEALVRLKRFFASNGVRKDAVL
>Mature_359_residues
PKSKRPPPQAESETISHAMSASVDESGASEVEEEIAEERDLDERQGGADEAGDAREGASEVAPDADDFRALLQAELTADT
IQHYLNRISVKPLLTVEEEQKYSRLAKAGEFEARQVMIERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEKFDPT
RGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKNSMNSGEAAERRDASIDDIAYLTGKTTDEV
TDILALNEHTASLDAPLDLDPASSLLDLLSDDQSQSPDAEVQHRELETLTRAWLARLSDKHRHVIERRFGLNHIEPATLE
ELADEMGLTRERVRQIQQEALVRLKRFFASNGVRKDAVL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in acid resistance [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=281, Percent_Identity=50.5338078291815, Blast_Score=268, Evalue=4e-73,
Organism=Escherichia coli, GI1789448, Length=264, Percent_Identity=38.2575757575758, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1789871, Length=286, Percent_Identity=27.2727272727273, Blast_Score=98, Evalue=7e-22,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 40586; Mature: 40455

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKSKRPPPQAESETISHAMSASVDESGASEVEEEIAEERDLDERQGGADEAGDAREGAS
CCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCCCHHCCHH
EVAPDADDFRALLQAELTADTIQHYLNRISVKPLLTVEEEQKYSRLAKAGEFEARQVMIE
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHCCCHHHHHHHHH
RNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEKFDPTRGFRFSTYATWWIRQSIER
HCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHH
AIMNQARTVRLPVHVIRELNQVLRAKRHLEKNSMNSGEAAERRDASIDDIAYLTGKTTDE
HHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHCCCCHHH
VTDILALNEHTASLDAPLDLDPASSLLDLLSDDQSQSPDAEVQHRELETLTRAWLARLSD
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KHRHVIERRFGLNHIEPATLEELADEMGLTRERVRQIQQEALVRLKRFFASNGVRKDAVL
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure 
PKSKRPPPQAESETISHAMSASVDESGASEVEEEIAEERDLDERQGGADEAGDAREGAS
CCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCCCHHCCHH
EVAPDADDFRALLQAELTADTIQHYLNRISVKPLLTVEEEQKYSRLAKAGEFEARQVMIE
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHCCCHHHHHHHHH
RNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEKFDPTRGFRFSTYATWWIRQSIER
HCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHH
AIMNQARTVRLPVHVIRELNQVLRAKRHLEKNSMNSGEAAERRDASIDDIAYLTGKTTDE
HHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHCCCCHHH
VTDILALNEHTASLDAPLDLDPASSLLDLLSDDQSQSPDAEVQHRELETLTRAWLARLSD
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KHRHVIERRFGLNHIEPATLEELADEMGLTRERVRQIQQEALVRLKRFFASNGVRKDAVL
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7959068; 10984043 [H]