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The map label for this gene is ansA [H]

Identifier: 91783355

GI number: 91783355

Start: 2198974

End: 2200014

Strand: Direct

Name: ansA [H]

Synonym: Bxe_A2464

Alternate gene names: 91783355

Gene position: 2198974-2200014 (Clockwise)

Preceding gene: 91783353

Following gene: 91783360

Centisome position: 44.92

GC content: 67.24

Gene sequence:

>1041_bases
ATGAATACTTCGATTCCTTCCTCCACCACATCTTCAGACGAAGGCGCCACGCTGCTGCCGCGCATCGCCGTGCTGGCAAC
CGGCGGCACGATTGCCGGCGCGGCTGCGGACGCCACCAATACGTCCGGCTACCAGGCCGGCGTGGTCGGCGTCGAGCAAT
TGCTGGCGGTGGTGCCGGCTTTGTCCGCGGTGGCGCGCGTCGCGCCCGAGCAGATTGCCAGCATCGACAGCAAGGACATG
GCGTTGCCGCTTTGGACCACGCTCGCACAACGCATCAACGCGCTGCTCGCCGACGACGACATCGACGGCGTGGTGGTCAC
GCACGGCACGGACACGCTCGAAGAAACCGCCTACCTGCTGCACCTGACCATCAAGTCGGACAAACCGGTCGTGCTGACGG
CGGCCATGCGCCCGGCCTCGGCGCTCTCCGCCGACGGTCCGCTGAATCTGCTCAACGCCGTGACGGTCGCGGCGCACGTG
GCGTCGCGCGGGCAGGGCGTACTGGTGGCCTTCAACAACAGGATTCACAGCGCGCGCGACGTGGTGAAGACGAGCACCTA
CGCGGTCGATGCATTCCATTCGCCGGAAATCGGCGCGCTCGGCTGGGTGCAGGACGGCCGCGTCGAATTCCAGCGCAGCG
TGGTGCGGCCGCATACGCTCGCCACCGAATTCGTGATCGGCGCGAAGTGGCCGCACGTGGAGATCGTCGTGAGCTACGCG
GGCGTTTCGCGGATTGCCGTGGATGCGCTGGTTGCCGCCGGCGTGCGCGGTATCGTGGTGGCAGGCACGGGCAACGGTTC
GATTCATGCCTCGGTGCAGCAGGCGCTGGCCGATGCCGCCTCGCAGGGCGTAGCGGTTGTGCGTGCGTCGCGCGTGGGTT
CGGGGCATGTGATGCGCAACGGCGCGGCCGCCGACGACGCGCTCGGCTTCGTCAGTGCGGGTTCGCTGAATCCGTACAAG
GCGCGTGTGTTGCTGATGCTGGCATTGGCCGCGGGCGCCGCGGGGCCGATGGTGTTGCAGAAGATATTCGATACGTACTG
A

Upstream 100 bases:

>100_bases
TTTCGTTCGCGCAGCCATTGGCTCAATCCGTCCAGGAGTTTGAAGCCATTTCTTGCGTGACGGCATGGCAGCACCCGGCG
CATCGCTTACAATCGCCGCC

Downstream 100 bases:

>100_bases
TCGATTGAACGCGGACGGCCTGGTTCAAAGTCAGAACGGTGTCGCGCACATTAAGCCTGAATGACAACGCGGCGCCCGGG
TCATTGACCGGGCGCCGCGT

Product: L-asparaginase, type II

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MNTSIPSSTTSSDEGATLLPRIAVLATGGTIAGAAADATNTSGYQAGVVGVEQLLAVVPALSAVARVAPEQIASIDSKDM
ALPLWTTLAQRINALLADDDIDGVVVTHGTDTLEETAYLLHLTIKSDKPVVLTAAMRPASALSADGPLNLLNAVTVAAHV
ASRGQGVLVAFNNRIHSARDVVKTSTYAVDAFHSPEIGALGWVQDGRVEFQRSVVRPHTLATEFVIGAKWPHVEIVVSYA
GVSRIAVDALVAAGVRGIVVAGTGNGSIHASVQQALADAASQGVAVVRASRVGSGHVMRNGAAADDALGFVSAGSLNPYK
ARVLLMLALAAGAAGPMVLQKIFDTY

Sequences:

>Translated_346_residues
MNTSIPSSTTSSDEGATLLPRIAVLATGGTIAGAAADATNTSGYQAGVVGVEQLLAVVPALSAVARVAPEQIASIDSKDM
ALPLWTTLAQRINALLADDDIDGVVVTHGTDTLEETAYLLHLTIKSDKPVVLTAAMRPASALSADGPLNLLNAVTVAAHV
ASRGQGVLVAFNNRIHSARDVVKTSTYAVDAFHSPEIGALGWVQDGRVEFQRSVVRPHTLATEFVIGAKWPHVEIVVSYA
GVSRIAVDALVAAGVRGIVVAGTGNGSIHASVQQALADAASQGVAVVRASRVGSGHVMRNGAAADDALGFVSAGSLNPYK
ARVLLMLALAAGAAGPMVLQKIFDTY
>Mature_346_residues
MNTSIPSSTTSSDEGATLLPRIAVLATGGTIAGAAADATNTSGYQAGVVGVEQLLAVVPALSAVARVAPEQIASIDSKDM
ALPLWTTLAQRINALLADDDIDGVVVTHGTDTLEETAYLLHLTIKSDKPVVLTAAMRPASALSADGPLNLLNAVTVAAHV
ASRGQGVLVAFNNRIHSARDVVKTSTYAVDAFHSPEIGALGWVQDGRVEFQRSVVRPHTLATEFVIGAKWPHVEIVVSYA
GVSRIAVDALVAAGVRGIVVAGTGNGSIHASVQQALADAASQGVAVVRASRVGSGHVMRNGAAADDALGFVSAGSLNPYK
ARVLLMLALAAGAAGPMVLQKIFDTY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=227, Percent_Identity=29.0748898678414, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1789327, Length=335, Percent_Identity=48.955223880597, Blast_Score=284, Evalue=5e-78,
Organism=Escherichia coli, GI1788065, Length=296, Percent_Identity=30.0675675675676, Blast_Score=102, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17558280, Length=310, Percent_Identity=28.3870967741935, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI193207372, Length=310, Percent_Identity=28.3870967741935, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17558278, Length=310, Percent_Identity=28.3870967741935, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI193207374, Length=310, Percent_Identity=28.3870967741935, Blast_Score=81, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6323189, Length=329, Percent_Identity=37.6899696048632, Blast_Score=200, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6323187, Length=329, Percent_Identity=37.6899696048632, Blast_Score=200, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6323186, Length=329, Percent_Identity=37.6899696048632, Blast_Score=200, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6323184, Length=329, Percent_Identity=37.6899696048632, Blast_Score=200, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6320527, Length=322, Percent_Identity=34.472049689441, Blast_Score=180, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24639609, Length=250, Percent_Identity=27.2, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24645530, Length=256, Percent_Identity=28.125, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35378; Mature: 35378

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSIPSSTTSSDEGATLLPRIAVLATGGTIAGAAADATNTSGYQAGVVGVEQLLAVVPA
CCCCCCCCCCCCCCCCCHHCEEEEEECCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
LSAVARVAPEQIASIDSKDMALPLWTTLAQRINALLADDDIDGVVVTHGTDTLEETAYLL
HHHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHCCEEEE
HLTIKSDKPVVLTAAMRPASALSADGPLNLLNAVTVAAHVASRGQGVLVAFNNRIHSARD
EEEECCCCCEEEEECCCCCHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHH
VVKTSTYAVDAFHSPEIGALGWVQDGRVEFQRSVVRPHTLATEFVIGAKWPHVEIVVSYA
HHHHCCEEEECCCCCCCCCEECCCCCHHHHHHHHCCCHHHHHHEEECCCCCCEEEEEEEC
GVSRIAVDALVAAGVRGIVVAGTGNGSIHASVQQALADAASQGVAVVRASRVGSGHVMRN
CHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEC
GAAADDALGFVSAGSLNPYKARVLLMLALAAGAAGPMVLQKIFDTY
CCCCCHHHCEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MNTSIPSSTTSSDEGATLLPRIAVLATGGTIAGAAADATNTSGYQAGVVGVEQLLAVVPA
CCCCCCCCCCCCCCCCCHHCEEEEEECCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
LSAVARVAPEQIASIDSKDMALPLWTTLAQRINALLADDDIDGVVVTHGTDTLEETAYLL
HHHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHCCEEEE
HLTIKSDKPVVLTAAMRPASALSADGPLNLLNAVTVAAHVASRGQGVLVAFNNRIHSARD
EEEECCCCCEEEEECCCCCHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHH
VVKTSTYAVDAFHSPEIGALGWVQDGRVEFQRSVVRPHTLATEFVIGAKWPHVEIVVSYA
HHHHCCEEEECCCCCCCCCEECCCCCHHHHHHHHCCCHHHHHHEEECCCCCCEEEEEEEC
GVSRIAVDALVAAGVRGIVVAGTGNGSIHASVQQALADAASQGVAVVRASRVGSGHVMRN
CHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEC
GAAADDALGFVSAGSLNPYKARVLLMLALAAGAAGPMVLQKIFDTY
CCCCCHHHCEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8898907; 14500908 [H]