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The map label for this gene is dmlR [H]

Identifier: 91783360

GI number: 91783360

Start: 2205157

End: 2206071

Strand: Direct

Name: dmlR [H]

Synonym: Bxe_A2459

Alternate gene names: 91783360

Gene position: 2205157-2206071 (Clockwise)

Preceding gene: 91783355

Following gene: 91783361

Centisome position: 45.04

GC content: 65.9

Gene sequence:

>915_bases
ATGGATCGCTTCAAACAGATCGAAACCTTCGTGCGCGTCGCGGACGCAGGCAGCCTCGCGGCGGCGGCGCTTGAGGAGGG
CGTGTCGCCTGTGATCCTCGGCCGCCGCATCGACGCGCTGGAAAAGCGCCTCGGCGTCAAGCTGATGTACCGCTCGACAC
GCCGGCTGGTGGTGAGCGAAGACGGCGCCGCGTTTCTGGAGCGCTGCCGGGGCCTGCTCACCGAATGGGATCAGGCGGAA
AACGAACTGAGCGCCGGGCGGCGCGCGATCAACGGCCATCTGATCGTGTCGGCGCCGGCAGCGTTCGGACGCAAGCACGT
CGCGCCGCTCGCGCCGGCATTTCTCGCCGACAAGCCGGAATTGCAGGTGTCGTTCAATCTGACCGACCGGGTGGTGGATC
TGGTGCGCGAGGGTTACGACCTGTCGATCCGCATCGGCGGCGCGGTCGATCCGAATTTCGTCGCGGTCAAGCTGGCGTCG
AACCGGCGCGTGGTGTGCGGCACGCCGGAGTATTTCCGTCGGCACGGCAGGCCGAAAACGCTCGAAGACCTGCCGCAGCA
CAACTGTCTGGCGTTCAATCTGCAGGGCGGACAGAACCGCGGCTGGTACTTCCGCCGCAACGGCAAGCTGGCGACGGTGC
GGGTGGGCGGCACGCTCGACTGCAACGACGGCGAGCTGCTGCACCGCTGGGTGTCGGAAGGGCTCGGGCTCGGCTGGCGC
TCCACGTGGGAAATCCAGCAGCAACTGGCGCGCGGCGAACTGGAAACCGTGCTCGACGAGTTCGCGCTGCCCGACTACGA
CATTCTCGCGGTCTATCCGCAGCAGCGGTATGTGCCGGCCAAGGTGCGGTACTTCATCGATTATCTGAAAGACGTATACG
CCAGCGGAGATTACTGGAACCGGCCGGCTTACTAG

Upstream 100 bases:

>100_bases
TGGGACCGGAGTAAGCGCTGAAGCGCTAACTGCGGTCGACTGCGCTGCATGGGACCGCAGTAAGCGCTAAAGCGCTAACT
GCGGTCGACAGGAGACGGAA

Downstream 100 bases:

>100_bases
ACTTAGGCGAGGCGGCGCCCGGCACCCCGGCACCGCCGGCACGCCGGCGGTCCTGCATCCACCGGTCCGGCCAGACTTTT
TTGGGATTGCGCGCGGTGTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MDRFKQIETFVRVADAGSLAAAALEEGVSPVILGRRIDALEKRLGVKLMYRSTRRLVVSEDGAAFLERCRGLLTEWDQAE
NELSAGRRAINGHLIVSAPAAFGRKHVAPLAPAFLADKPELQVSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLAS
NRRVVCGTPEYFRRHGRPKTLEDLPQHNCLAFNLQGGQNRGWYFRRNGKLATVRVGGTLDCNDGELLHRWVSEGLGLGWR
STWEIQQQLARGELETVLDEFALPDYDILAVYPQQRYVPAKVRYFIDYLKDVYASGDYWNRPAY

Sequences:

>Translated_304_residues
MDRFKQIETFVRVADAGSLAAAALEEGVSPVILGRRIDALEKRLGVKLMYRSTRRLVVSEDGAAFLERCRGLLTEWDQAE
NELSAGRRAINGHLIVSAPAAFGRKHVAPLAPAFLADKPELQVSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLAS
NRRVVCGTPEYFRRHGRPKTLEDLPQHNCLAFNLQGGQNRGWYFRRNGKLATVRVGGTLDCNDGELLHRWVSEGLGLGWR
STWEIQQQLARGELETVLDEFALPDYDILAVYPQQRYVPAKVRYFIDYLKDVYASGDYWNRPAY
>Mature_304_residues
MDRFKQIETFVRVADAGSLAAAALEEGVSPVILGRRIDALEKRLGVKLMYRSTRRLVVSEDGAAFLERCRGLLTEWDQAE
NELSAGRRAINGHLIVSAPAAFGRKHVAPLAPAFLADKPELQVSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLAS
NRRVVCGTPEYFRRHGRPKTLEDLPQHNCLAFNLQGGQNRGWYFRRNGKLATVRVGGTLDCNDGELLHRWVSEGLGLGWR
STWEIQQQLARGELETVLDEFALPDYDILAVYPQQRYVPAKVRYFIDYLKDVYASGDYWNRPAY

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=291, Percent_Identity=37.1134020618557, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1789440, Length=285, Percent_Identity=31.9298245614035, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=31.5972222222222, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1787128, Length=295, Percent_Identity=29.1525423728814, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1789639, Length=294, Percent_Identity=27.2108843537415, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1787589, Length=286, Percent_Identity=27.972027972028, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=25.938566552901, Blast_Score=117, Evalue=9e-28,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34252; Mature: 34252

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRFKQIETFVRVADAGSLAAAALEEGVSPVILGRRIDALEKRLGVKLMYRSTRRLVVSE
CCHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHCEEEEEECCCEEEEEC
DGAAFLERCRGLLTEWDQAENELSAGRRAINGHLIVSAPAAFGRKHVAPLAPAFLADKPE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHCCCCCCCCCHHHHCCCCC
LQVSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLASNRRVVCGTPEYFRRHGRPKT
EEEEECHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCEEEECCHHHHHHCCCCCC
LEDLPQHNCLAFNLQGGQNRGWYFRRNGKLATVRVGGTLDCNDGELLHRWVSEGLGLGWR
HHHCCCCCEEEEEECCCCCCCEEEEECCEEEEEEECCEEECCCHHHHHHHHHHHCCCCCC
STWEIQQQLARGELETVLDEFALPDYDILAVYPQQRYVPAKVRYFIDYLKDVYASGDYWN
CHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCC
RPAY
CCCC
>Mature Secondary Structure
MDRFKQIETFVRVADAGSLAAAALEEGVSPVILGRRIDALEKRLGVKLMYRSTRRLVVSE
CCHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHCEEEEEECCCEEEEEC
DGAAFLERCRGLLTEWDQAENELSAGRRAINGHLIVSAPAAFGRKHVAPLAPAFLADKPE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHCCCCCCCCCHHHHCCCCC
LQVSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLASNRRVVCGTPEYFRRHGRPKT
EEEEECHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCEEEECCHHHHHHCCCCCC
LEDLPQHNCLAFNLQGGQNRGWYFRRNGKLATVRVGGTLDCNDGELLHRWVSEGLGLGWR
HHHCCCCCEEEEEECCCCCCCEEEEECCEEEEEEECCEEECCCHHHHHHHHHHHCCCCCC
STWEIQQQLARGELETVLDEFALPDYDILAVYPQQRYVPAKVRYFIDYLKDVYASGDYWN
CHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCC
RPAY
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]