| Definition | Burkholderia xenovorans LB400 chromosome 1, complete sequence. |
|---|---|
| Accession | NC_007951 |
| Length | 4,895,836 |
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The map label for this gene is yngF [H]
Identifier: 91782262
GI number: 91782262
Start: 988602
End: 989381
Strand: Direct
Name: yngF [H]
Synonym: Bxe_A3572
Alternate gene names: 91782262
Gene position: 988602-989381 (Clockwise)
Preceding gene: 91782261
Following gene: 91782263
Centisome position: 20.19
GC content: 64.1
Gene sequence:
>780_bases ATGATCGAGTTTGAACTTAGCCATAACGGTGCGGTGGCTGTTGTTACGCTGACGCGTCCCCCGGCAAACGCGTTTACGCC CGATGGCCTGCGGCAGTTGCAGCAAGTCATCCGAACCTGTGACGAACAACTCCATGTGCGCGCGGTGGTCATCACCGGTA GCGGCCCGAAATTCTTCAGCGCGGGGGCGGATCTGAATACCTTCGCCTCGGGAGATCGCGACCTTGCGCGCGAGGCAGCG TCTGCCTTCGAAGCCGTGCAGAATGCCCGCCCTGTCGTGATTGCTGCGATCAACGGCTATGCAATGGGCGGTGGACTGGA ATGCGCGCTCGCTTGTGATATCCGCATTGCGGAGGAGCACGCAGTGCTGGCGCTGCCCGAGACGGCTGTGGGTCTGTTGC CATGTGGTTGTGGTACGCAAACGCTCCCATGGCTCGTCGGCGAAGGCTGGGCGAAGCGGATCATCCTGACCGGCGAGCGC GTCAATGCGGCTACTGCGCTGCGCATCGGACTGGTGGAGGACGTGGTTGCAAGAGGCGGCGCGCGCGAGGCCGCGCTCGC GATGGCCGCGCGTGTCGCGTCTCTTAGTCCGCAGGCGGTGTCGTTCAGCAAGGAACTGATCCATCAGGGGCGCAACGGCG TGCCGCGGGAGGCGGCACTTGCCGTTGAGCGTGAGCGTTTCGTCGATCTGTCCGGTTCGCCCGACCAGCTCGAAGGCGTG AACGCCTTCCTCGAAAAGCGCGCACCGCGATGGAGCACGACAGCGGAGAGTGGCGCATGA
Upstream 100 bases:
>100_bases AGGACTTTCCTGAACTGCGCTGAACATGTATCCGCATGTGGTAGTGCTGCGTGCGGTATCTCAGCATTGGCGACCGTTCC CTGGTCTACGGAGAAGCTGA
Downstream 100 bases:
>100_bases ACGCGACGCCTGATTTCCAGTCTTCCTCGAAGCCCATTTCGCCGGAGACAGGCAGCACCGGAAGCGAAGTGCTGTTTCAG GTGGTGAACCGCGTCGCTGT
Product: enoyl-CoA hydratase
Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O
Alternate protein names: NA
Number of amino acids: Translated: 259; Mature: 259
Protein sequence:
>259_residues MIEFELSHNGAVAVVTLTRPPANAFTPDGLRQLQQVIRTCDEQLHVRAVVITGSGPKFFSAGADLNTFASGDRDLAREAA SAFEAVQNARPVVIAAINGYAMGGGLECALACDIRIAEEHAVLALPETAVGLLPCGCGTQTLPWLVGEGWAKRIILTGER VNAATALRIGLVEDVVARGGAREAALAMAARVASLSPQAVSFSKELIHQGRNGVPREAALAVERERFVDLSGSPDQLEGV NAFLEKRAPRWSTTAESGA
Sequences:
>Translated_259_residues MIEFELSHNGAVAVVTLTRPPANAFTPDGLRQLQQVIRTCDEQLHVRAVVITGSGPKFFSAGADLNTFASGDRDLAREAA SAFEAVQNARPVVIAAINGYAMGGGLECALACDIRIAEEHAVLALPETAVGLLPCGCGTQTLPWLVGEGWAKRIILTGER VNAATALRIGLVEDVVARGGAREAALAMAARVASLSPQAVSFSKELIHQGRNGVPREAALAVERERFVDLSGSPDQLEGV NAFLEKRAPRWSTTAESGA >Mature_259_residues MIEFELSHNGAVAVVTLTRPPANAFTPDGLRQLQQVIRTCDEQLHVRAVVITGSGPKFFSAGADLNTFASGDRDLAREAA SAFEAVQNARPVVIAAINGYAMGGGLECALACDIRIAEEHAVLALPETAVGLLPCGCGTQTLPWLVGEGWAKRIILTGER VNAATALRIGLVEDVVARGGAREAALAMAARVASLSPQAVSFSKELIHQGRNGVPREAALAVERERFVDLSGSPDQLEGV NAFLEKRAPRWSTTAESGA
Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]
Homologues:
Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=34.2741935483871, Blast_Score=126, Evalue=2e-29, Organism=Homo sapiens, GI68989263, Length=290, Percent_Identity=33.1034482758621, Blast_Score=115, Evalue=4e-26, Organism=Homo sapiens, GI20127408, Length=177, Percent_Identity=35.0282485875706, Blast_Score=96, Evalue=4e-20, Organism=Homo sapiens, GI4502327, Length=254, Percent_Identity=33.4645669291339, Blast_Score=92, Evalue=4e-19, Organism=Homo sapiens, GI31542718, Length=246, Percent_Identity=29.6747967479675, Blast_Score=89, Evalue=5e-18, Organism=Homo sapiens, GI37594471, Length=180, Percent_Identity=30.5555555555556, Blast_Score=80, Evalue=2e-15, Organism=Homo sapiens, GI37594469, Length=183, Percent_Identity=30.6010928961749, Blast_Score=80, Evalue=2e-15, Organism=Homo sapiens, GI221136756, Length=239, Percent_Identity=27.6150627615063, Blast_Score=73, Evalue=3e-13, Organism=Homo sapiens, GI221136753, Length=239, Percent_Identity=27.6150627615063, Blast_Score=73, Evalue=3e-13, Organism=Homo sapiens, GI221307494, Length=239, Percent_Identity=27.6150627615063, Blast_Score=73, Evalue=3e-13, Organism=Homo sapiens, GI261878539, Length=190, Percent_Identity=33.1578947368421, Blast_Score=73, Evalue=3e-13, Organism=Homo sapiens, GI157694516, Length=250, Percent_Identity=25.6, Blast_Score=72, Evalue=6e-13, Organism=Homo sapiens, GI213417737, Length=250, Percent_Identity=25.6, Blast_Score=72, Evalue=6e-13, Organism=Homo sapiens, GI70995211, Length=208, Percent_Identity=33.1730769230769, Blast_Score=71, Evalue=9e-13, Organism=Homo sapiens, GI150378541, Length=242, Percent_Identity=30.5785123966942, Blast_Score=71, Evalue=9e-13, Organism=Homo sapiens, GI62530384, Length=207, Percent_Identity=28.5024154589372, Blast_Score=70, Evalue=1e-12, Organism=Escherichia coli, GI1787659, Length=257, Percent_Identity=37.7431906614786, Blast_Score=146, Evalue=1e-36, Organism=Escherichia coli, GI221142681, Length=256, Percent_Identity=34.375, Blast_Score=137, Evalue=5e-34, Organism=Escherichia coli, GI1788682, Length=189, Percent_Identity=38.0952380952381, Blast_Score=109, Evalue=2e-25, Organism=Escherichia coli, GI1790281, Length=151, Percent_Identity=35.7615894039735, Blast_Score=82, Evalue=5e-17, Organism=Escherichia coli, GI1788597, Length=258, Percent_Identity=25.5813953488372, Blast_Score=79, Evalue=3e-16, Organism=Escherichia coli, GI87082183, Length=238, Percent_Identity=26.0504201680672, Blast_Score=72, Evalue=3e-14, Organism=Escherichia coli, GI1787660, Length=264, Percent_Identity=30.6818181818182, Blast_Score=70, Evalue=1e-13, Organism=Caenorhabditis elegans, GI25145438, Length=255, Percent_Identity=33.7254901960784, Blast_Score=124, Evalue=5e-29, Organism=Caenorhabditis elegans, GI17554946, Length=257, Percent_Identity=33.0739299610895, Blast_Score=123, Evalue=1e-28, Organism=Caenorhabditis elegans, GI17540714, Length=243, Percent_Identity=31.2757201646091, Blast_Score=102, Evalue=2e-22, Organism=Caenorhabditis elegans, GI17558304, Length=176, Percent_Identity=34.0909090909091, Blast_Score=99, Evalue=2e-21, Organism=Caenorhabditis elegans, GI17536985, Length=224, Percent_Identity=29.4642857142857, Blast_Score=89, Evalue=3e-18, Organism=Caenorhabditis elegans, GI25144276, Length=176, Percent_Identity=34.0909090909091, Blast_Score=87, Evalue=8e-18, Organism=Caenorhabditis elegans, GI17508951, Length=180, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=9e-18, Organism=Caenorhabditis elegans, GI17508953, Length=176, Percent_Identity=34.0909090909091, Blast_Score=84, Evalue=6e-17, Organism=Caenorhabditis elegans, GI17534483, Length=218, Percent_Identity=31.1926605504587, Blast_Score=84, Evalue=7e-17, Organism=Caenorhabditis elegans, GI17549921, Length=221, Percent_Identity=28.9592760180996, Blast_Score=77, Evalue=7e-15, Organism=Caenorhabditis elegans, GI25144157, Length=183, Percent_Identity=31.6939890710383, Blast_Score=74, Evalue=6e-14, Organism=Caenorhabditis elegans, GI25144160, Length=183, Percent_Identity=31.6939890710383, Blast_Score=74, Evalue=7e-14, Organism=Caenorhabditis elegans, GI17560910, Length=226, Percent_Identity=27.4336283185841, Blast_Score=70, Evalue=1e-12, Organism=Caenorhabditis elegans, GI17535521, Length=261, Percent_Identity=26.0536398467433, Blast_Score=70, Evalue=1e-12, Organism=Caenorhabditis elegans, GI17540306, Length=261, Percent_Identity=25.2873563218391, Blast_Score=69, Evalue=3e-12, Organism=Drosophila melanogaster, GI20129971, Length=258, Percent_Identity=32.1705426356589, Blast_Score=114, Evalue=5e-26, Organism=Drosophila melanogaster, GI24653477, Length=258, Percent_Identity=32.1705426356589, Blast_Score=114, Evalue=5e-26, Organism=Drosophila melanogaster, GI24653139, Length=260, Percent_Identity=32.6923076923077, Blast_Score=114, Evalue=8e-26, Organism=Drosophila melanogaster, GI28571729, Length=164, Percent_Identity=30.4878048780488, Blast_Score=75, Evalue=4e-14, Organism=Drosophila melanogaster, GI28571730, Length=164, Percent_Identity=30.4878048780488, Blast_Score=75, Evalue=4e-14, Organism=Drosophila melanogaster, GI24583165, Length=251, Percent_Identity=24.7011952191235, Blast_Score=74, Evalue=8e-14, Organism=Drosophila melanogaster, GI21357171, Length=171, Percent_Identity=35.0877192982456, Blast_Score=74, Evalue=1e-13, Organism=Drosophila melanogaster, GI24650670, Length=206, Percent_Identity=30.0970873786408, Blast_Score=71, Evalue=6e-13, Organism=Drosophila melanogaster, GI19922422, Length=249, Percent_Identity=23.2931726907631, Blast_Score=70, Evalue=1e-12, Organism=Drosophila melanogaster, GI19921018, Length=247, Percent_Identity=25.1012145748988, Blast_Score=70, Evalue=1e-12, Organism=Drosophila melanogaster, GI19920382, Length=229, Percent_Identity=31.0043668122271, Blast_Score=68, Evalue=7e-12, Organism=Drosophila melanogaster, GI24583077, Length=177, Percent_Identity=34.4632768361582, Blast_Score=67, Evalue=1e-11, Organism=Drosophila melanogaster, GI24583079, Length=177, Percent_Identity=34.4632768361582, Blast_Score=67, Evalue=1e-11, Organism=Drosophila melanogaster, GI19921000, Length=177, Percent_Identity=34.4632768361582, Blast_Score=67, Evalue=1e-11, Organism=Drosophila melanogaster, GI24654903, Length=180, Percent_Identity=26.1111111111111, Blast_Score=65, Evalue=5e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR014748 - InterPro: IPR001753 - InterPro: IPR018376 [H]
Pfam domain/function: PF00378 ECH [H]
EC number: 4.2.1.17
Molecular weight: Translated: 27234; Mature: 27234
Theoretical pI: Translated: 5.39; Mature: 5.39
Prosite motif: PS00166 ENOYL_COA_HYDRATASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.9 %Cys (Translated Protein) 1.2 %Met (Translated Protein) 3.1 %Cys+Met (Translated Protein) 1.9 %Cys (Mature Protein) 1.2 %Met (Mature Protein) 3.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MIEFELSHNGAVAVVTLTRPPANAFTPDGLRQLQQVIRTCDEQLHVRAVVITGSGPKFFS CEEEEECCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCEEE AGADLNTFASGDRDLAREAASAFEAVQNARPVVIAAINGYAMGGGLECALACDIRIAEEH CCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCEEECCCCEEEEEEEEEEECCC AVLALPETAVGLLPCGCGTQTLPWLVGEGWAKRIILTGERVNAATALRIGLVEDVVARGG EEEECCCHHHEEEECCCCCCCCCHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCC AREAALAMAARVASLSPQAVSFSKELIHQGRNGVPREAALAVERERFVDLSGSPDQLEGV CHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEECCCCCHHHHHH NAFLEKRAPRWSTTAESGA HHHHHHCCCCCCCCCCCCC >Mature Secondary Structure MIEFELSHNGAVAVVTLTRPPANAFTPDGLRQLQQVIRTCDEQLHVRAVVITGSGPKFFS CEEEEECCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCEEE AGADLNTFASGDRDLAREAASAFEAVQNARPVVIAAINGYAMGGGLECALACDIRIAEEH CCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCEEECCCCEEEEEEEEEEECCC AVLALPETAVGLLPCGCGTQTLPWLVGEGWAKRIILTGERVNAATALRIGLVEDVVARGG EEEECCCHHHEEEECCCCCCCCCHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCC AREAALAMAARVASLSPQAVSFSKELIHQGRNGVPREAALAVERERFVDLSGSPDQLEGV CHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEECCCCCHHHHHH NAFLEKRAPRWSTTAESGA HHHHHHCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]
Substrates: (3S)-3-hydroxyacyl-CoA
Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O
General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 9387222; 9384377 [H]