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The map label for this gene is yngF [H]

Identifier: 91782263

GI number: 91782263

Start: 989378

End: 990667

Strand: Direct

Name: yngF [H]

Synonym: Bxe_A3571

Alternate gene names: 91782263

Gene position: 989378-990667 (Clockwise)

Preceding gene: 91782262

Following gene: 91782264

Centisome position: 20.21

GC content: 62.25

Gene sequence:

>1290_bases
ATGAACGCGACGCCTGATTTCCAGTCTTCCTCGAAGCCCATTTCGCCGGAGACAGGCAGCACCGGAAGCGAAGTGCTGTT
TCAGGTGGTGAACCGCGTCGCTGTCATCACCTTGAATCGACCCGCCGCGCTCAATGCACTGTCGCACTTGATGGTGCGTG
ATCTCGCGAATCTGCTCGAACGAGTGCGCAACGACGACCGGATCGTTGCACTCGTGCTGCGCGGTGGAGATCCGAAGGGT
TTTTGCGCAGGCGGTGATGTGCGGGCGTTGTACCAGCTCGCGCGTCGTGGAGAGCGCGACGGCGAGAATGGCTGGCTGCA
GTTTTTCGTTGATGAATATCAGCTTGATCATGCATTGCACAGATTCCCGAAGCCGGTTGTCGCGCTGCTGGATGGGGTCA
CGATGGGCGGGGGGATGGGGCTCGGCCAGGCAGCGCAGATCCGGGTCGTGACGACCCGCACGAAAATCGCGATGCCGGAG
ACCCGCATCGGGATGGTGCCGGATGTTGGCGCGACCCGTTTTCTTGCGGCGATGCCGCTGGAAACCGAACTGTACGTTGG
GCTGACGGGTGTCACGTTGTCGGGTGCTGATGCGGTGGCGTGCGGCCTGGCAGACCTCTGCGTACCGGGCGAATGGTTCG
ACGATTTCGAGGGCCGGCTGGAGCGGATGAGAATTGAGGGAAATGTGCTGGAGGCGCTGAGAGCGGTTTTTGCTCCGGTG
GGTGTCGAGGGAATACCGAAGGCCACATTGCCCGAGTACACGCCGCTGATCTCGCGGCATTTCGACCGGCGCGCGGGAAT
TGAGCAGATCGTGAGCACACTCTCTGCCGGGCTTGCCCGGCACCCTCCACAACCTGAAGCAGACTGGTTGCTCGCGACGC
TCGACGCACTGACGCAGTACTCGCCAACGATGTTGATGGTAACGCGTGAGGCGCTACTGCGGGGGCGCCAGATGACACTC
GCAGAATGTTTCCGGATGGAACTCGGCATTGTCTCGCGTGCGATCGGGGAGGGAGAGTTCTGTGAAGGCGTGCGTGCGCA
TCTCGTTGACAAGGACCGCAAGCCCCGGTGGGCTCCGGCGAAGCTCGCCGAGGTTCGTGCTGAGCGCGTACAGCATTTTC
TGTCTTCGCCGTGGCGCAACAACACGCATCCGCTTGCCGGTCTGCGCGATTGCGATTGGCCGGCGCGCGAGACCGCTAAA
CCAGGGCGCGGGCAATGCGCTGGAGCTAATGGCGAGGACAGCCTGTGCCTATCCCACTCCGGAGAGATGGGGCGGGTAAG
AGGAATGTGA

Upstream 100 bases:

>100_bases
GTTTCGTCGATCTGTCCGGTTCGCCCGACCAGCTCGAAGGCGTGAACGCCTTCCTCGAAAAGCGCGCACCGCGATGGAGC
ACGACAGCGGAGAGTGGCGC

Downstream 100 bases:

>100_bases
TCAAAGAGAATGGAGCGACGATGCAATGAAGATTCTGGTGGCGGTCAAGCGGGTTGTGGATGCCAATGTAAAAGTACAGT
CCAGGTCGGACGGGACGGGA

Product: putative enoyl-CoA hydratase/isomerase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MNATPDFQSSSKPISPETGSTGSEVLFQVVNRVAVITLNRPAALNALSHLMVRDLANLLERVRNDDRIVALVLRGGDPKG
FCAGGDVRALYQLARRGERDGENGWLQFFVDEYQLDHALHRFPKPVVALLDGVTMGGGMGLGQAAQIRVVTTRTKIAMPE
TRIGMVPDVGATRFLAAMPLETELYVGLTGVTLSGADAVACGLADLCVPGEWFDDFEGRLERMRIEGNVLEALRAVFAPV
GVEGIPKATLPEYTPLISRHFDRRAGIEQIVSTLSAGLARHPPQPEADWLLATLDALTQYSPTMLMVTREALLRGRQMTL
AECFRMELGIVSRAIGEGEFCEGVRAHLVDKDRKPRWAPAKLAEVRAERVQHFLSSPWRNNTHPLAGLRDCDWPARETAK
PGRGQCAGANGEDSLCLSHSGEMGRVRGM

Sequences:

>Translated_429_residues
MNATPDFQSSSKPISPETGSTGSEVLFQVVNRVAVITLNRPAALNALSHLMVRDLANLLERVRNDDRIVALVLRGGDPKG
FCAGGDVRALYQLARRGERDGENGWLQFFVDEYQLDHALHRFPKPVVALLDGVTMGGGMGLGQAAQIRVVTTRTKIAMPE
TRIGMVPDVGATRFLAAMPLETELYVGLTGVTLSGADAVACGLADLCVPGEWFDDFEGRLERMRIEGNVLEALRAVFAPV
GVEGIPKATLPEYTPLISRHFDRRAGIEQIVSTLSAGLARHPPQPEADWLLATLDALTQYSPTMLMVTREALLRGRQMTL
AECFRMELGIVSRAIGEGEFCEGVRAHLVDKDRKPRWAPAKLAEVRAERVQHFLSSPWRNNTHPLAGLRDCDWPARETAK
PGRGQCAGANGEDSLCLSHSGEMGRVRGM
>Mature_429_residues
MNATPDFQSSSKPISPETGSTGSEVLFQVVNRVAVITLNRPAALNALSHLMVRDLANLLERVRNDDRIVALVLRGGDPKG
FCAGGDVRALYQLARRGERDGENGWLQFFVDEYQLDHALHRFPKPVVALLDGVTMGGGMGLGQAAQIRVVTTRTKIAMPE
TRIGMVPDVGATRFLAAMPLETELYVGLTGVTLSGADAVACGLADLCVPGEWFDDFEGRLERMRIEGNVLEALRAVFAPV
GVEGIPKATLPEYTPLISRHFDRRAGIEQIVSTLSAGLARHPPQPEADWLLATLDALTQYSPTMLMVTREALLRGRQMTL
AECFRMELGIVSRAIGEGEFCEGVRAHLVDKDRKPRWAPAKLAEVRAERVQHFLSSPWRNNTHPLAGLRDCDWPARETAK
PGRGQCAGANGEDSLCLSHSGEMGRVRGM

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI37594471, Length=355, Percent_Identity=34.0845070422535, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI37594469, Length=306, Percent_Identity=32.6797385620915, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI194097323, Length=182, Percent_Identity=33.5164835164835, Blast_Score=86, Evalue=6e-17,
Organism=Escherichia coli, GI1788682, Length=186, Percent_Identity=27.9569892473118, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787659, Length=201, Percent_Identity=27.363184079602, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1788597, Length=164, Percent_Identity=29.2682926829268, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI221142681, Length=177, Percent_Identity=28.8135593220339, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI25144160, Length=372, Percent_Identity=36.2903225806452, Blast_Score=215, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI25144157, Length=372, Percent_Identity=36.2903225806452, Blast_Score=214, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI17540714, Length=180, Percent_Identity=26.1111111111111, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI25145438, Length=184, Percent_Identity=29.8913043478261, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17554946, Length=235, Percent_Identity=27.6595744680851, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI25144276, Length=182, Percent_Identity=27.4725274725275, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=182, Percent_Identity=27.4725274725275, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17508951, Length=182, Percent_Identity=27.4725274725275, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=357, Percent_Identity=30.2521008403361, Blast_Score=139, Evalue=8e-34,
Organism=Drosophila melanogaster, GI28571730, Length=371, Percent_Identity=35.8490566037736, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI28571729, Length=371, Percent_Identity=35.8490566037736, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24653139, Length=201, Percent_Identity=32.8358208955224, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI20129971, Length=182, Percent_Identity=30.7692307692308, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653477, Length=182, Percent_Identity=30.7692307692308, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 46810; Mature: 46810

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATPDFQSSSKPISPETGSTGSEVLFQVVNRVAVITLNRPAALNALSHLMVRDLANLLE
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
RVRNDDRIVALVLRGGDPKGFCAGGDVRALYQLARRGERDGENGWLQFFVDEYQLDHALH
HHCCCCEEEEEEEECCCCCCEECCCCHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHH
RFPKPVVALLDGVTMGGGMGLGQAAQIRVVTTRTKIAMPETRIGMVPDVGATRFLAAMPL
HCCHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHCCC
ETELYVGLTGVTLSGADAVACGLADLCVPGEWFDDFEGRLERMRIEGNVLEALRAVFAPV
CCEEEEEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
GVEGIPKATLPEYTPLISRHFDRRAGIEQIVSTLSAGLARHPPQPEADWLLATLDALTQY
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
SPTMLMVTREALLRGRQMTLAECFRMELGIVSRAIGEGEFCEGVRAHLVDKDRKPRWAPA
CCHHHEEEHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHH
KLAEVRAERVQHFLSSPWRNNTHPLAGLRDCDWPARETAKPGRGQCAGANGEDSLCLSHS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCEEECCC
GEMGRVRGM
CCCCCCCCC
>Mature Secondary Structure
MNATPDFQSSSKPISPETGSTGSEVLFQVVNRVAVITLNRPAALNALSHLMVRDLANLLE
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
RVRNDDRIVALVLRGGDPKGFCAGGDVRALYQLARRGERDGENGWLQFFVDEYQLDHALH
HHCCCCEEEEEEEECCCCCCEECCCCHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHH
RFPKPVVALLDGVTMGGGMGLGQAAQIRVVTTRTKIAMPETRIGMVPDVGATRFLAAMPL
HCCHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHCCC
ETELYVGLTGVTLSGADAVACGLADLCVPGEWFDDFEGRLERMRIEGNVLEALRAVFAPV
CCEEEEEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
GVEGIPKATLPEYTPLISRHFDRRAGIEQIVSTLSAGLARHPPQPEADWLLATLDALTQY
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
SPTMLMVTREALLRGRQMTLAECFRMELGIVSRAIGEGEFCEGVRAHLVDKDRKPRWAPA
CCHHHEEEHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHH
KLAEVRAERVQHFLSSPWRNNTHPLAGLRDCDWPARETAKPGRGQCAGANGEDSLCLSHS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCEEECCC
GEMGRVRGM
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]