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The map label for this gene is emrA [H]

Identifier: 91782252

GI number: 91782252

Start: 974518

End: 975780

Strand: Reverse

Name: emrA [H]

Synonym: Bxe_A3583

Alternate gene names: 91782252

Gene position: 975780-974518 (Counterclockwise)

Preceding gene: 91782253

Following gene: 91782251

Centisome position: 19.93

GC content: 65.87

Gene sequence:

>1263_bases
ATGAGCACCACCCAAAAGCCCGCGGCTGCTACACAGCCGACTCAGCCGGCCCCGCAGCCGAGGCAACCGGCCCAACCGCC
GGCGAACAACGGCAAACGCAAGCGCACGATGACGCTGCTCGTCATCGTGATCCTGATCGCCGCGCTCGCCTACGGCCTGT
ACTACTTCCTCGTCGCGCGCTTCCACGAAGACACCGACGACGCGTACGTGAGCGGCAACGTCGTGCAGATCACGCCGCAA
GTCACGGGCACCGTGGTCGCCGTGAACGCGGACGACACGCAAGTGGTCAAGGCGGGCGATCCGCTCATCGTACTCGATCC
GGCCGACGCACGCGTGGCGCTCGAGCAGGCCGAGGCGAATCTCGCGCAAACGGTACGCCAGGTGCGCGGCCTGTTCGCCG
ACGACAACCAGTACCAGGCGCAAGTCGCACTGCGTCAGTCCGATCTGTCGCGCGCCCAGGACGACCTGAAGCGCCGTCTG
ACGGTCGCGCAAACCGGCGCGGTATCGCAGGAAGAAATCTCGCACGCCCGCGACGCGGTGAAGAGCGCGCAAGCCGCCGT
CGACGCCGCCCAGCAGCAACTGGAGTCGAACCGCGCGCTGACCGCCAACACCACGATCGCGAACCATCCGAACGTGCAGG
CCGCCGCCGCGAAGGTCCGCGACGCGTACCTGAACCACGCGCGCAACGTTCTGCCTGCGCCGGTGACCGGCTACGTCGCA
AAACGCTCGGTGCAGGTCGGCCAGCGCGTCTCGCCCGGCACCCCGTTGATGGCCATCGTGCCGCTTCACGACGCGTGGGT
CGACGCGAATTTCAAGGAAGTGCAGCTCAAGCACATGCGCATCGGTCAGCCGGTGGAACTGACAGCCGACGTGTACGGCT
CGTCGGTGCCGTATCACGGCAAGGTGGTCGGCTTCTCGGCGGGCACGGGTTCGGCGTTCTCGCTGCTGCCCGCGCAAAAC
GCCACCGGCAACTGGATCAAGGTGGTGCAGCGTCTGCCGGTGCGTATCGCGCTCGATCCGCAGGAACTCGACAAACACCC
GCTGCGCATCGGCCTGTCGATGCAAGCCGACGTGAACATCAAGGACGACACCGGCGGCCAGCTCGGCCAGGCGCCGAACA
CGGCCTACCAGACCAACGTCTTCGATAAATACGGCGACGAAGCCGACGCGGAAATCGCTCGCATCATCGCGGAAAACGCC
GGGCTCGAGACCACAACACAGAGAGTCGAACACCAAAAAAATCCCGGCCAGAAGCCACTGTAA

Upstream 100 bases:

>100_bases
GCCGCGACCGCCACGGCGAACTGAAGCCACGCCGCCGCAAACCACATTCGCACGAACATCCGAATAAACGACGACGCTTG
AGAGAACCCGGAGCACATCA

Downstream 100 bases:

>100_bases
CCCGTACCAAGACTGCTAAAGAGGCTTCTCCATGGCTCAATCAGCGCCCCCGCCACCGCCACCGGCACCCGCCCCCGAAG
CGCCGATCCATCACCCGCCG

Product: major facilitator superfamily efflux pump membrane fusion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MSTTQKPAAATQPTQPAPQPRQPAQPPANNGKRKRTMTLLVIVILIAALAYGLYYFLVARFHEDTDDAYVSGNVVQITPQ
VTGTVVAVNADDTQVVKAGDPLIVLDPADARVALEQAEANLAQTVRQVRGLFADDNQYQAQVALRQSDLSRAQDDLKRRL
TVAQTGAVSQEEISHARDAVKSAQAAVDAAQQQLESNRALTANTTIANHPNVQAAAAKVRDAYLNHARNVLPAPVTGYVA
KRSVQVGQRVSPGTPLMAIVPLHDAWVDANFKEVQLKHMRIGQPVELTADVYGSSVPYHGKVVGFSAGTGSAFSLLPAQN
ATGNWIKVVQRLPVRIALDPQELDKHPLRIGLSMQADVNIKDDTGGQLGQAPNTAYQTNVFDKYGDEADAEIARIIAENA
GLETTTQRVEHQKNPGQKPL

Sequences:

>Translated_420_residues
MSTTQKPAAATQPTQPAPQPRQPAQPPANNGKRKRTMTLLVIVILIAALAYGLYYFLVARFHEDTDDAYVSGNVVQITPQ
VTGTVVAVNADDTQVVKAGDPLIVLDPADARVALEQAEANLAQTVRQVRGLFADDNQYQAQVALRQSDLSRAQDDLKRRL
TVAQTGAVSQEEISHARDAVKSAQAAVDAAQQQLESNRALTANTTIANHPNVQAAAAKVRDAYLNHARNVLPAPVTGYVA
KRSVQVGQRVSPGTPLMAIVPLHDAWVDANFKEVQLKHMRIGQPVELTADVYGSSVPYHGKVVGFSAGTGSAFSLLPAQN
ATGNWIKVVQRLPVRIALDPQELDKHPLRIGLSMQADVNIKDDTGGQLGQAPNTAYQTNVFDKYGDEADAEIARIIAENA
GLETTTQRVEHQKNPGQKPL
>Mature_419_residues
STTQKPAAATQPTQPAPQPRQPAQPPANNGKRKRTMTLLVIVILIAALAYGLYYFLVARFHEDTDDAYVSGNVVQITPQV
TGTVVAVNADDTQVVKAGDPLIVLDPADARVALEQAEANLAQTVRQVRGLFADDNQYQAQVALRQSDLSRAQDDLKRRLT
VAQTGAVSQEEISHARDAVKSAQAAVDAAQQQLESNRALTANTTIANHPNVQAAAAKVRDAYLNHARNVLPAPVTGYVAK
RSVQVGQRVSPGTPLMAIVPLHDAWVDANFKEVQLKHMRIGQPVELTADVYGSSVPYHGKVVGFSAGTGSAFSLLPAQNA
TGNWIKVVQRLPVRIALDPQELDKHPLRIGLSMQADVNIKDDTGGQLGQAPNTAYQTNVFDKYGDEADAEIARIIAENAG
LETTTQRVEHQKNPGQKPL

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=343, Percent_Identity=53.3527696793003, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1788711, Length=378, Percent_Identity=44.1798941798942, Blast_Score=328, Evalue=5e-91,
Organism=Escherichia coli, GI87081951, Length=309, Percent_Identity=29.7734627831715, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790519, Length=323, Percent_Identity=30.030959752322, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789637, Length=326, Percent_Identity=29.1411042944785, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1790024, Length=235, Percent_Identity=25.1063829787234, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 45205; Mature: 45074

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTQKPAAATQPTQPAPQPRQPAQPPANNGKRKRTMTLLVIVILIAALAYGLYYFLVAR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FHEDTDDAYVSGNVVQITPQVTGTVVAVNADDTQVVKAGDPLIVLDPADARVALEQAEAN
HCCCCCCEEECCCEEEEECCCCEEEEEEECCCCEEEECCCCEEEECCCCHHHHHHHHHHH
LAQTVRQVRGLFADDNQYQAQVALRQSDLSRAQDDLKRRLTVAQTGAVSQEEISHARDAV
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
KSAQAAVDAAQQQLESNRALTANTTIANHPNVQAAAAKVRDAYLNHARNVLPAPVTGYVA
HHHHHHHHHHHHHHHCCCCEEECCEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
KRSVQVGQRVSPGTPLMAIVPLHDAWVDANFKEVQLKHMRIGQPVELTADVYGSSVPYHG
HHHHHHCCCCCCCCCEEEEEECCHHHHCCCHHHHHHHHHCCCCCCEEEHHHCCCCCCCCC
KVVGFSAGTGSAFSLLPAQNATGNWIKVVQRLPVRIALDPQELDKHPLRIGLSMQADVNI
EEEEEECCCCCEEEEECCCCCCCHHHHHHHHCCCEEEECHHHHCCCCEEEEEEEEECCEE
KDDTGGQLGQAPNTAYQTNVFDKYGDEADAEIARIIAENAGLETTTQRVEHQKNPGQKPL
CCCCCCCCCCCCCCCEECCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
STTQKPAAATQPTQPAPQPRQPAQPPANNGKRKRTMTLLVIVILIAALAYGLYYFLVAR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FHEDTDDAYVSGNVVQITPQVTGTVVAVNADDTQVVKAGDPLIVLDPADARVALEQAEAN
HCCCCCCEEECCCEEEEECCCCEEEEEEECCCCEEEECCCCEEEECCCCHHHHHHHHHHH
LAQTVRQVRGLFADDNQYQAQVALRQSDLSRAQDDLKRRLTVAQTGAVSQEEISHARDAV
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
KSAQAAVDAAQQQLESNRALTANTTIANHPNVQAAAAKVRDAYLNHARNVLPAPVTGYVA
HHHHHHHHHHHHHHHCCCCEEECCEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
KRSVQVGQRVSPGTPLMAIVPLHDAWVDANFKEVQLKHMRIGQPVELTADVYGSSVPYHG
HHHHHHCCCCCCCCCEEEEEECCHHHHCCCHHHHHHHHHCCCCCCEEEHHHCCCCCCCCC
KVVGFSAGTGSAFSLLPAQNATGNWIKVVQRLPVRIALDPQELDKHPLRIGLSMQADVNI
EEEEEECCCCCEEEEECCCCCCCHHHHHHHHCCCEEEECHHHHCCCCEEEEEEEEECCEE
KDDTGGQLGQAPNTAYQTNVFDKYGDEADAEIARIIAENAGLETTTQRVEHQKNPGQKPL
CCCCCCCCCCCCCCCEECCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]