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The map label for this gene is emrB [H]

Identifier: 91782251

GI number: 91782251

Start: 972891

End: 974486

Strand: Reverse

Name: emrB [H]

Synonym: Bxe_A3584

Alternate gene names: 91782251

Gene position: 974486-972891 (Counterclockwise)

Preceding gene: 91782252

Following gene: 91782250

Centisome position: 19.9

GC content: 61.09

Gene sequence:

>1596_bases
ATGGCTCAATCAGCGCCCCCGCCACCGCCACCGGCACCCGCCCCCGAAGCGCCGATCCATCACCCGCCGCTCAAAGGTGG
GCAGCTCGTTGTCGCCACCATTGTCGTCGCGCTTGCCACGTTCATGACGGTGCTCGACACGTCGATTGCCAACGTCGCCA
TTCCGAACCTCTCGGGTAACCTGGGCGTCTCCGTCGACGAAGGCACCTGGGTGATCACGATGTTCGCCGCCGCCAATGCG
GTATCCATTCCCCTGACCGGATGGCTCACGCAACGCATCGGCCAGATCAAACTGTTCGTCGGCGCGATCCTGATGTTCGT
TCTGGCGTCATGGCTGTGCGGTATCGCACCCACGCTGCCGTTCCTGCTTGCCGCACGGATCCTGCAAGGCGTGGTAGCCG
GCCCGCTTATCCCGCTGTCGCAGGCTGTCCTGCTCGGCTCCTACCCGAAAGAAAAATCCGGCACGGCGCTGGCCCTGTGG
GCGACGACTGCCACCGTCGGCCCGATCGCGGGCCCGATTCTCGGCGGCTGGATTACCGACAACTACAGCTGGTCGTGGAT
CTTTTACGTCAACATTCCCGTCGGCCTCGTCGCGGCCAGTGTCATCTGGATGATCTACCGTGACCGCGAAACGCAAACAA
GGAAACTGCCGATTGACGTCGTCGGACTCGGCCTGCTGATCGCATGGGTCGCTTCGCTGCAGATCATGCTCGACAAGGGC
AAGGATCTCGACTGGTTTTCATCACCGCTCATCGTCGGCCTTGGCATTGCAGCACTGATCAGTTTCGCTTTCTTTCTCGT
GTGGGAGCTGACCGAGGAGCACCCCATTGTCGACCTGCGGCTGTTTGGGCAGCGCAACTTCCTTGGGGGCACCATTGCTG
TCTCCGTCTCCTACGCGCTCTACTTCGGCAATCTCGTGCTACTGCCGCAGTGGATGCAGGAATATCTCGGCTACCGCGCA
TTCGATGCCGGACTCGTGACCGCACCAGTCGGTGTCTTTGCGGTCATTCTCTCGCCGATCGTCGGGCGCATGCTGCCCTA
TACGGACGCACGGAAGATAGCGACCTGTGCGTTCATCGGGTTCGCGGGTGTGTTTTACATGCGCACGAACTACGTCACAG
ATATCGACACCTGGCATCTCGTGCTACCCACGTTGCTCCAGGGTATTCCGACCGTCCTGTGGTTCGTGCCCCTGACGGCC
ATCATCCTGTCGGGATTGCCGCCGGAGCGCATTCCGGCGGCAGCCGGCCTGTCCAATTTCGTCCGGATATTCTGCGGCGC
GGCGGGGACCTCCATCGCAGGCACCGTCTGGAACAATCGGACCATCCTGCATCACGAGCGCCTGACTGAACAAGCCAGTA
TCAACAATCCGCTGTTCAACCAGCAGATCGCCTCGTCCCAATCGCTACTGCATCTTGACACGCAATCAGCGCAGGCGCTT
TTCGATGCCTCGGTGAACAGTCAGGCGGCGATCATGGGACTGAACGACTTCTTCCACATTTCGGTCTTCATCTTCATCCT
CATTATTCCGCTGATCTGGATCACGCGGCCGGTAAAGGGCGGTGGCGGCGCTGATGCGGCCGCTGGCGCGCACTGA

Upstream 100 bases:

>100_bases
AACGCCGGGCTCGAGACCACAACACAGAGAGTCGAACACCAAAAAAATCCCGGCCAGAAGCCACTGTAACCCGTACCAAG
ACTGCTAAAGAGGCTTCTCC

Downstream 100 bases:

>100_bases
ACTCGCTCCGGAAACATTCCCCATGCGCAATCTTCCAGATTCAGGCTGCGGGTACCGCGTCAGCAGCGTTCTTGCCGCTG
TCCGTATCCCCCTCCCAGGG

Product: major facilitator superfamily multidrug efflux transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MAQSAPPPPPPAPAPEAPIHHPPLKGGQLVVATIVVALATFMTVLDTSIANVAIPNLSGNLGVSVDEGTWVITMFAAANA
VSIPLTGWLTQRIGQIKLFVGAILMFVLASWLCGIAPTLPFLLAARILQGVVAGPLIPLSQAVLLGSYPKEKSGTALALW
ATTATVGPIAGPILGGWITDNYSWSWIFYVNIPVGLVAASVIWMIYRDRETQTRKLPIDVVGLGLLIAWVASLQIMLDKG
KDLDWFSSPLIVGLGIAALISFAFFLVWELTEEHPIVDLRLFGQRNFLGGTIAVSVSYALYFGNLVLLPQWMQEYLGYRA
FDAGLVTAPVGVFAVILSPIVGRMLPYTDARKIATCAFIGFAGVFYMRTNYVTDIDTWHLVLPTLLQGIPTVLWFVPLTA
IILSGLPPERIPAAAGLSNFVRIFCGAAGTSIAGTVWNNRTILHHERLTEQASINNPLFNQQIASSQSLLHLDTQSAQAL
FDASVNSQAAIMGLNDFFHISVFIFILIIPLIWITRPVKGGGGADAAAGAH

Sequences:

>Translated_531_residues
MAQSAPPPPPPAPAPEAPIHHPPLKGGQLVVATIVVALATFMTVLDTSIANVAIPNLSGNLGVSVDEGTWVITMFAAANA
VSIPLTGWLTQRIGQIKLFVGAILMFVLASWLCGIAPTLPFLLAARILQGVVAGPLIPLSQAVLLGSYPKEKSGTALALW
ATTATVGPIAGPILGGWITDNYSWSWIFYVNIPVGLVAASVIWMIYRDRETQTRKLPIDVVGLGLLIAWVASLQIMLDKG
KDLDWFSSPLIVGLGIAALISFAFFLVWELTEEHPIVDLRLFGQRNFLGGTIAVSVSYALYFGNLVLLPQWMQEYLGYRA
FDAGLVTAPVGVFAVILSPIVGRMLPYTDARKIATCAFIGFAGVFYMRTNYVTDIDTWHLVLPTLLQGIPTVLWFVPLTA
IILSGLPPERIPAAAGLSNFVRIFCGAAGTSIAGTVWNNRTILHHERLTEQASINNPLFNQQIASSQSLLHLDTQSAQAL
FDASVNSQAAIMGLNDFFHISVFIFILIIPLIWITRPVKGGGGADAAAGAH
>Mature_530_residues
AQSAPPPPPPAPAPEAPIHHPPLKGGQLVVATIVVALATFMTVLDTSIANVAIPNLSGNLGVSVDEGTWVITMFAAANAV
SIPLTGWLTQRIGQIKLFVGAILMFVLASWLCGIAPTLPFLLAARILQGVVAGPLIPLSQAVLLGSYPKEKSGTALALWA
TTATVGPIAGPILGGWITDNYSWSWIFYVNIPVGLVAASVIWMIYRDRETQTRKLPIDVVGLGLLIAWVASLQIMLDKGK
DLDWFSSPLIVGLGIAALISFAFFLVWELTEEHPIVDLRLFGQRNFLGGTIAVSVSYALYFGNLVLLPQWMQEYLGYRAF
DAGLVTAPVGVFAVILSPIVGRMLPYTDARKIATCAFIGFAGVFYMRTNYVTDIDTWHLVLPTLLQGIPTVLWFVPLTAI
ILSGLPPERIPAAAGLSNFVRIFCGAAGTSIAGTVWNNRTILHHERLTEQASINNPLFNQQIASSQSLLHLDTQSAQALF
DASVNSQAAIMGLNDFFHISVFIFILIIPLIWITRPVKGGGGADAAAGAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=500, Percent_Identity=49.4, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI1788710, Length=498, Percent_Identity=43.3734939759036, Blast_Score=417, Evalue=1e-117,
Organism=Escherichia coli, GI1788392, Length=406, Percent_Identity=30.0492610837438, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI1790195, Length=385, Percent_Identity=27.5324675324675, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI87081983, Length=420, Percent_Identity=23.8095238095238, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321663, Length=181, Percent_Identity=27.0718232044199, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=187, Percent_Identity=25.1336898395722, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56917; Mature: 56786

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQSAPPPPPPAPAPEAPIHHPPLKGGQLVVATIVVALATFMTVLDTSIANVAIPNLSGN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCC
LGVSVDEGTWVITMFAAANAVSIPLTGWLTQRIGQIKLFVGAILMFVLASWLCGIAPTLP
CCEEECCCCEEEEEEECCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLLAARILQGVVAGPLIPLSQAVLLGSYPKEKSGTALALWATTATVGPIAGPILGGWITD
HHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEEEEEHHCCCCCHHHHHCCEEEC
NYSWSWIFYVNIPVGLVAASVIWMIYRDRETQTRKLPIDVVGLGLLIAWVASLQIMLDKG
CCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
KDLDWFSSPLIVGLGIAALISFAFFLVWELTEEHPIVDLRLFGQRNFLGGTIAVSVSYAL
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEHHHHHHH
YFGNLVLLPQWMQEYLGYRAFDAGLVTAPVGVFAVILSPIVGRMLPYTDARKIATCAFIG
HHCCCEEEHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
FAGVFYMRTNYVTDIDTWHLVLPTLLQGIPTVLWFVPLTAIILSGLPPERIPAAAGLSNF
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHH
VRIFCGAAGTSIAGTVWNNRTILHHERLTEQASINNPLFNQQIASSQSLLHLDTQSAQAL
HHHHHCCCCCCEEEEEECCCEEEEHHHHHHHHHCCCCCHHHHHHCCCCEEEECCHHHHHH
FDASVNSQAAIMGLNDFFHISVFIFILIIPLIWITRPVKGGGGADAAAGAH
HHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AQSAPPPPPPAPAPEAPIHHPPLKGGQLVVATIVVALATFMTVLDTSIANVAIPNLSGN
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCC
LGVSVDEGTWVITMFAAANAVSIPLTGWLTQRIGQIKLFVGAILMFVLASWLCGIAPTLP
CCEEECCCCEEEEEEECCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLLAARILQGVVAGPLIPLSQAVLLGSYPKEKSGTALALWATTATVGPIAGPILGGWITD
HHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEEEEEHHCCCCCHHHHHCCEEEC
NYSWSWIFYVNIPVGLVAASVIWMIYRDRETQTRKLPIDVVGLGLLIAWVASLQIMLDKG
CCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
KDLDWFSSPLIVGLGIAALISFAFFLVWELTEEHPIVDLRLFGQRNFLGGTIAVSVSYAL
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEHHHHHHH
YFGNLVLLPQWMQEYLGYRAFDAGLVTAPVGVFAVILSPIVGRMLPYTDARKIATCAFIG
HHCCCEEEHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
FAGVFYMRTNYVTDIDTWHLVLPTLLQGIPTVLWFVPLTAIILSGLPPERIPAAAGLSNF
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHH
VRIFCGAAGTSIAGTVWNNRTILHHERLTEQASINNPLFNQQIASSQSLLHLDTQSAQAL
HHHHHCCCCCCEEEEEECCCEEEEHHHHHHHHHCCCCCHHHHHHCCCCEEEECCHHHHHH
FDASVNSQAAIMGLNDFFHISVFIFILIIPLIWITRPVKGGGGADAAAGAH
HHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]