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The map label for this gene is kstD [H]

Identifier: 91782228

GI number: 91782228

Start: 946813

End: 948603

Strand: Direct

Name: kstD [H]

Synonym: Bxe_A3607

Alternate gene names: 91782228

Gene position: 946813-948603 (Clockwise)

Preceding gene: 91782227

Following gene: 91782229

Centisome position: 19.34

GC content: 62.26

Gene sequence:

>1791_bases
ATGGGGAAAGGTGCTCAGGTGGTCAACGAATTCGATGTGATCGTGGTGGGCGCGGGTGCCGGCGGTATGGCCGCGGCGGT
CAGCGCGGCGAAGAACGGGGCGAGTGTGATTGTCATCGAAAAGGACAAGGTACTCGGCGGGACGACTGCGCGCTCGGGCG
GCGTGCTGTGGATTCCGTGTCATCCGTTCTCGCAGCAGAGCCCTGCCGAAGATTCGATCGAGAACGCCACGACCTACCTT
CGTCATGAGGCGGGGCGATACTTCAATGCGGAGAAGGTGCAGGCGTTTCTTCACAACGGGCCGCGCATGGTCGACTGGTT
CCAGCGCGAAACCGACGTGAAGTTCGTCTCCGTGCCGGAGTTCTCGGACTACCATCCGGATGCGGCCGGCGGTCTGCCCG
GCGGCCGTTCGATCCTTGCCGCGCCGTTCGACGGCAGGGAGTTAGGCAAAAAGATCGAATGGCTGCGCCCGCCGCTGCGC
GAGATTACCTTCGTGGGCATGATGTTCAATGCAAGCAAGGAGATCAGCCAGTTCTTCAACGTGACACGTTCGCTGGCCTC
CGCATGGTATGTGACGAAACGCCTTGTTACGCACGCGCGCGAAATGCTGACATACGGACGCGCCATGCGCCTGACCAACG
GCAATGCACTCGCTGCGCGTCTCGCGAAAAGCGCCTTCGACTGCGACATTCCATTTCGCCTCGACACGCGTGTCGAACGT
CTGCTGATGGAAGGCAGGCGCGTGGCAGGCGTCGCGGTAAATTCGGGCGGCGAAAGCATGAACCTGTACGCCCGTACGGG
CGTCATCCTCGCTGGCGGCGGGTTTCCGCAGGACGTGGAGCGTCGCAAGGCGCTATTTCCGCACGCACCGAATGGAACCG
AGCATCTGTCGCCCGCGCCGGCCAGCAACACGGGTGACGGACTTCGCCTCGCTGCGAATGCCGGCGCGGCGCTCGAACTC
GACCTGCCTAATGCCGCCGCCTGGATACCCGTTTCGAAGGTGCCGTATCGCAACGGCGAGTCCGGTGTATTTCCTCATCT
GATCGACCGGTACAAACCAGGCGTCATCATGGTGACGCGAAACGGACTTCGCTTTACTAACGAATCCAACTCGTATCACG
ACGTCGGCATGGCAATGCAGAAGGCGTGCCGGGATAGCGATGCGACAGAGGCTTTCCTGATCTGCGATCACCGAACCTTG
CGACGCTACGGCCTGGGATTCGTCAAGCCGTTTCCCATTCCGTTTGGTCCGCATCTGCGCAGCGGCTACCTGAAGCGCGG
ACGCACACTAGCCGAACTGGCGGATGCGGCCGGCATCGATGCTGCGGCCTTCGAACGTACGGTCGCGACTTTCAACGAAG
GAGCGGTGCATGGTGAAGACCATCAGTTCGGACGCGGCAATTCCGCGTACAACCGTTTTCTTGGCGATGCAGAACATCAG
CCGAATCCTTGTGTCGCGCCGATCCAGGACGGGCCGTTCTACGCGATTCGAACAGTAATCGGCGATCTTGGCACCTTCGC
CGGGATTCGCACGGATGGCTGTGCGCGGGCGCTCGATTCAAGCGGGCATGTGGTGCCCGGTTTGTACGCGGTGGGAAACG
ACGCCGCAAGCGTAATGGGTGGGAATTATCCGGGCGGCGGCATTACCCTGGGACCGGCGATGACCTTCGGTTTTCTCGCG
GGCGAGCACGCTGCGAAAACCGCGCGCGGCGCGCGGGAAAACGCGCTAAGTCTGGATATGCCGGTAGCCGAGGTCAGCGT
GCTGGCGCGCAGCAGCGAGCAGGCGCCCTGA

Upstream 100 bases:

>100_bases
GAGGGGCGCACTCGTGCTGTCGTTCTTTCTGAAGCCAAACAGGTTTCTGTCGGGCGAGCTGGCAGGACAGGAACTCAAGT
AAAGCGGCACGCGGGTCGAC

Downstream 100 bases:

>100_bases
CACCGCAAAACGGAAACGGCGTCTCGCTGAAAAGGATGCGAATGGAACCCGAGCTGAAGGCGTTGATAGAACAACTAAGC
CAGGCAGGGCGGCGGCCGTT

Product: putative dehydrogenase

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 596; Mature: 595

Protein sequence:

>596_residues
MGKGAQVVNEFDVIVVGAGAGGMAAAVSAAKNGASVIVIEKDKVLGGTTARSGGVLWIPCHPFSQQSPAEDSIENATTYL
RHEAGRYFNAEKVQAFLHNGPRMVDWFQRETDVKFVSVPEFSDYHPDAAGGLPGGRSILAAPFDGRELGKKIEWLRPPLR
EITFVGMMFNASKEISQFFNVTRSLASAWYVTKRLVTHAREMLTYGRAMRLTNGNALAARLAKSAFDCDIPFRLDTRVER
LLMEGRRVAGVAVNSGGESMNLYARTGVILAGGGFPQDVERRKALFPHAPNGTEHLSPAPASNTGDGLRLAANAGAALEL
DLPNAAAWIPVSKVPYRNGESGVFPHLIDRYKPGVIMVTRNGLRFTNESNSYHDVGMAMQKACRDSDATEAFLICDHRTL
RRYGLGFVKPFPIPFGPHLRSGYLKRGRTLAELADAAGIDAAAFERTVATFNEGAVHGEDHQFGRGNSAYNRFLGDAEHQ
PNPCVAPIQDGPFYAIRTVIGDLGTFAGIRTDGCARALDSSGHVVPGLYAVGNDAASVMGGNYPGGGITLGPAMTFGFLA
GEHAAKTARGARENALSLDMPVAEVSVLARSSEQAP

Sequences:

>Translated_596_residues
MGKGAQVVNEFDVIVVGAGAGGMAAAVSAAKNGASVIVIEKDKVLGGTTARSGGVLWIPCHPFSQQSPAEDSIENATTYL
RHEAGRYFNAEKVQAFLHNGPRMVDWFQRETDVKFVSVPEFSDYHPDAAGGLPGGRSILAAPFDGRELGKKIEWLRPPLR
EITFVGMMFNASKEISQFFNVTRSLASAWYVTKRLVTHAREMLTYGRAMRLTNGNALAARLAKSAFDCDIPFRLDTRVER
LLMEGRRVAGVAVNSGGESMNLYARTGVILAGGGFPQDVERRKALFPHAPNGTEHLSPAPASNTGDGLRLAANAGAALEL
DLPNAAAWIPVSKVPYRNGESGVFPHLIDRYKPGVIMVTRNGLRFTNESNSYHDVGMAMQKACRDSDATEAFLICDHRTL
RRYGLGFVKPFPIPFGPHLRSGYLKRGRTLAELADAAGIDAAAFERTVATFNEGAVHGEDHQFGRGNSAYNRFLGDAEHQ
PNPCVAPIQDGPFYAIRTVIGDLGTFAGIRTDGCARALDSSGHVVPGLYAVGNDAASVMGGNYPGGGITLGPAMTFGFLA
GEHAAKTARGARENALSLDMPVAEVSVLARSSEQAP
>Mature_595_residues
GKGAQVVNEFDVIVVGAGAGGMAAAVSAAKNGASVIVIEKDKVLGGTTARSGGVLWIPCHPFSQQSPAEDSIENATTYLR
HEAGRYFNAEKVQAFLHNGPRMVDWFQRETDVKFVSVPEFSDYHPDAAGGLPGGRSILAAPFDGRELGKKIEWLRPPLRE
ITFVGMMFNASKEISQFFNVTRSLASAWYVTKRLVTHAREMLTYGRAMRLTNGNALAARLAKSAFDCDIPFRLDTRVERL
LMEGRRVAGVAVNSGGESMNLYARTGVILAGGGFPQDVERRKALFPHAPNGTEHLSPAPASNTGDGLRLAANAGAALELD
LPNAAAWIPVSKVPYRNGESGVFPHLIDRYKPGVIMVTRNGLRFTNESNSYHDVGMAMQKACRDSDATEAFLICDHRTLR
RYGLGFVKPFPIPFGPHLRSGYLKRGRTLAELADAAGIDAAAFERTVATFNEGAVHGEDHQFGRGNSAYNRFLGDAEHQP
NPCVAPIQDGPFYAIRTVIGDLGTFAGIRTDGCARALDSSGHVVPGLYAVGNDAASVMGGNYPGGGITLGPAMTFGFLAG
EHAAKTARGARENALSLDMPVAEVSVLARSSEQAP

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 63748; Mature: 63617

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKGAQVVNEFDVIVVGAGAGGMAAAVSAAKNGASVIVIEKDKVLGGTTARSGGVLWIPC
CCCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEEEEC
HPFSQQSPAEDSIENATTYLRHEAGRYFNAEKVQAFLHNGPRMVDWFQRETDVKFVSVPE
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHCCCEEEEECCC
FSDYHPDAAGGLPGGRSILAAPFDGRELGKKIEWLRPPLREITFVGMMFNASKEISQFFN
CCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCHHHHEEEHHHHCCHHHHHHHHH
VTRSLASAWYVTKRLVTHAREMLTYGRAMRLTNGNALAARLAKSAFDCDIPFRLDTRVER
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHCCCCCCEEHHHHHHH
LLMEGRRVAGVAVNSGGESMNLYARTGVILAGGGFPQDVERRKALFPHAPNGTEHLSPAP
HHHCCCEEEEEEEECCCCCEEEEEECCEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCC
ASNTGDGLRLAANAGAALELDLPNAAAWIPVSKVPYRNGESGVFPHLIDRYKPGVIMVTR
CCCCCCCEEEEECCCCEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHCCCCEEEEEC
NGLRFTNESNSYHDVGMAMQKACRDSDATEAFLICDHRTLRRYGLGFVKPFPIPFGPHLR
CCEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHCCCCCCCCCCCCCCCHH
SGYLKRGRTLAELADAAGIDAAAFERTVATFNEGAVHGEDHQFGRGNSAYNRFLGDAEHQ
HHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCC
PNPCVAPIQDGPFYAIRTVIGDLGTFAGIRTDGCARALDSSGHVVPGLYAVGNDAASVMG
CCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEECCCHHHHCC
GNYPGGGITLGPAMTFGFLAGEHAAKTARGARENALSLDMPVAEVSVLARSSEQAP
CCCCCCCEEECHHHHHHHHHCHHHHHHHCCCCCCCEECCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GKGAQVVNEFDVIVVGAGAGGMAAAVSAAKNGASVIVIEKDKVLGGTTARSGGVLWIPC
CCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEEEEC
HPFSQQSPAEDSIENATTYLRHEAGRYFNAEKVQAFLHNGPRMVDWFQRETDVKFVSVPE
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHCCCEEEEECCC
FSDYHPDAAGGLPGGRSILAAPFDGRELGKKIEWLRPPLREITFVGMMFNASKEISQFFN
CCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCHHHHEEEHHHHCCHHHHHHHHH
VTRSLASAWYVTKRLVTHAREMLTYGRAMRLTNGNALAARLAKSAFDCDIPFRLDTRVER
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHCCCCCCEEHHHHHHH
LLMEGRRVAGVAVNSGGESMNLYARTGVILAGGGFPQDVERRKALFPHAPNGTEHLSPAP
HHHCCCEEEEEEEECCCCCEEEEEECCEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCC
ASNTGDGLRLAANAGAALELDLPNAAAWIPVSKVPYRNGESGVFPHLIDRYKPGVIMVTR
CCCCCCCEEEEECCCCEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHCCCCEEEEEC
NGLRFTNESNSYHDVGMAMQKACRDSDATEAFLICDHRTLRRYGLGFVKPFPIPFGPHLR
CCEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHCCCCCCCCCCCCCCCHH
SGYLKRGRTLAELADAAGIDAAAFERTVATFNEGAVHGEDHQFGRGNSAYNRFLGDAEHQ
HHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCC
PNPCVAPIQDGPFYAIRTVIGDLGTFAGIRTDGCARALDSSGHVVPGLYAVGNDAASVMG
CCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEECCCHHHHCC
GNYPGGGITLGPAMTFGFLAGEHAAKTARGARENALSLDMPVAEVSVLARSSEQAP
CCCCCCCEEECHHHHHHHHHCHHHHHHHCCCCCCCEECCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]