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The map label for this gene is fadN [H]

Identifier: 91781808

GI number: 91781808

Start: 472763

End: 475198

Strand: Direct

Name: fadN [H]

Synonym: Bxe_A4037

Alternate gene names: 91781808

Gene position: 472763-475198 (Clockwise)

Preceding gene: 91781807

Following gene: 91781809

Centisome position: 9.66

GC content: 64.24

Gene sequence:

>2436_bases
GTGAGCAATCTGATCATTCGCAAGGTAGCCGTGCTCGGCGCCGGCGTGATGGGCGCGCAGATCGCCGCGCACCTGATCAA
CGCCAAGGTGCCCGTGCTGCTGTTCGATCTGCCTGCCAAGGAAGGCCCGAAGAACGCGATCGCGCTGAAGGCGATCGAGA
ATCTGAAGAAGCTGTCGCCCGCGCCGTTCGGCGTGAAGGACGACGCGCAATACGTCCAGCCGGCCAACTACGACGACGAC
ATCGGGAAGCTCGCCGAATGCGACCTCGTGATCGAAGCGATCGCCGAGCGCATGGACTGGAAGCACGACCTGTACAGGAA
AGTAGCGCCGCATCTCGCGCCGAACGCGATTTTCGCGACCAACACGTCGGGCCTGTCGATCACCGCGCTCTCGGAAGGTT
TCCCGGATGAACTGAAGGCGCGCTTCTGCGGCGTGCACTTCTTCAATCCGCCGCGCTACATGCACCTGGTCGAACTGATC
CCGACCGCGACCACACGCCCGGAAATTCTCGACCAACTCGAGTCGTTCCTGACCAGCGTGGTCGGCAAAGGCGTCGTGCG
TGCAAAAGATACGCCGAACTTCATCGCCAACCGCGTGGGGATTTTCTCGATTCTCGCTGTCGTGACCGAAGCCGCGAAAT
ACGGCCTGCGTTTCGACGAAGTGGACGACCTGACCGGCGCACGTCTGGGCCGCGCGAAGTCGGCCACGTTCCGTACCGCG
GACGTGGTCGGTCTGGACACGATGGCGCACGTCATCAAGACGATGCAGGACACGCTGAAGGACGACCCGTTCTATCCGGT
CTACGAAACGCCGGCTGTGCTGGCCGAACTGGTGAAGAAGGGCGCGCTGGGCCAGAAAACTGGCGGCGGCTTCTACAGGA
AAGAAGGCAAGGCGATCAAGGTGCTCGACGCGAAAACGGGCGAGTACGTGGACGGCGGCGCGAAGGCGGACGAACTGGTC
GGCCGCATCCTGAAGCGCCCGCCGGCGGAGCGTCTGAAGCTGCTGCGCGAATCGGAGCATCCGCAGGCGCAGTTCCTGTG
GGCGATTTTCCGCGACGTGTATCACTATATCGGCGTGCATCTGGAATCGATCGCCGACAACGCGCGCGACGTCGATCTGG
CGATCCGCTGGGGCTTCGGCTGGAACGAAGGCCCGTTCGAAGGCTGGCAGACGGCCGGCTGGAAGCAGGTCGCCGAGTGG
GTGCAGGAAGACATCGCAGCCGGCAAGGCGCTCTCGAACGCGCCGCTGCCGTCGTGGGTGCTGGAAGGTCCGGTGGCCGA
GAAAGGCGGCGTGCATACCAACGAAGGTTCGTGGTCGCCGGCTTCGAAGACGTTCGTGCCGCGCTCGTCGCTGTCGGTCT
ACGACAAACAGGTGTTCCGTGCGCCGCTGGTCGGCGAAACCGCCGCTGATCCGAAGACCTACGGCAAGACGCTGTTCGAA
ACCGACGCCGTGCGCGCCTGGGTCGACGATCGGGCCGGCGAGAACGATGTGCTGATCGTGTCGTTCAAAAGCAAGATGAA
CACGATCGGACCGTCGGTGATCGACGGTCTCACGCAGGCGATCGAACTGGCCGAAAAGGACTACAAGGGTCTGGTGGTGT
GGCAGCCCACGTCGCTGAAACTCGGCACGCCGGGCGGCCCGTTCTCCGCGGGCGCCAACCTCGAGGAAGCGATGCCGGCC
TTCATGATGGGCGGCGCGAAGGGCATCGAGCCGTTCGTGAAGAAATTCCAGCAAGGCATGTTGCGCGTGAAGTATGCAAG
CGTGCCGGTCATTTCGGCCGTGTCGGGCATCGCGCTCGGCGGCGGTTGCGAACTGGCGCTGCATAGCGCGAAGCGGGTCG
CGCATATCGAGAGCTATCTCGGGCTGGTGGAAGTCGGCGTGGGTCTCGTGCCCGCCGGCGGCGGCCTGAAGGAAGCGGCG
CTGCGCGCGGCGGAAGCGGCGACGCAAGTCGGCGCGACCAACGACCTGCTGAAGTTCGTGCAGAAGTCGTTCGAAAACGC
GGCGATGGCGAAGGTCTCGGCCTCCGCGCTTGACGCCCGCGCGATGGGCTACCTGAAGCCGTCCGATACGATCGTCTTCA
ACGTGTTCGAGTTGCTCGACGTCGCGAAGAAGGAGGCGCGCGCGTTGTCGGCGGCGGGTTATCGTCCGCCGCTGCGGGTC
ACGCAAGTGCCGGTGGCCGGCCGCTCGGCGATCGCGACCATCAAGGCGTCGCTCGTCAACATGCGCGATGGCCGTTTTAT
CAGCGAGCACGACTTCCTGATTGCGAGTCGCATCGCGGAAGCGGTGTGCGGCGGCGACGTGGAAGCGGGCAGCCTGGTCG
ATGAAGAATGGCTGCTGCAACTCGAGCGTCGTGCGTTCGTCGATCTGCTCGGCACGCAAAAGACGCAGGAACGGATCATG
GGCATGCTGCAAACCGGCAAGCCGGTGCGCAACTGA

Upstream 100 bases:

>100_bases
GGCGCCACGCGCTGGAAAATGCGTGGCGCCTGCGTCAGCCACCTGTCGCGAAGCGTCCAACCAGGCTTCGCGACCCCGCA
TATCTCCCCGGAGGAACGAC

Downstream 100 bases:

>100_bases
GCGACGAGCCAATAGGAGATTCAAATGGCAAAGCAATTGCAAGACGCATACATCGTCGCCGCGAGCCGCACGCCGATCGG
CAAGGCGCCGCGCGGGATGT

Product: 3-hydroxyacyl-CoA dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 811; Mature: 810

Protein sequence:

>811_residues
MSNLIIRKVAVLGAGVMGAQIAAHLINAKVPVLLFDLPAKEGPKNAIALKAIENLKKLSPAPFGVKDDAQYVQPANYDDD
IGKLAECDLVIEAIAERMDWKHDLYRKVAPHLAPNAIFATNTSGLSITALSEGFPDELKARFCGVHFFNPPRYMHLVELI
PTATTRPEILDQLESFLTSVVGKGVVRAKDTPNFIANRVGIFSILAVVTEAAKYGLRFDEVDDLTGARLGRAKSATFRTA
DVVGLDTMAHVIKTMQDTLKDDPFYPVYETPAVLAELVKKGALGQKTGGGFYRKEGKAIKVLDAKTGEYVDGGAKADELV
GRILKRPPAERLKLLRESEHPQAQFLWAIFRDVYHYIGVHLESIADNARDVDLAIRWGFGWNEGPFEGWQTAGWKQVAEW
VQEDIAAGKALSNAPLPSWVLEGPVAEKGGVHTNEGSWSPASKTFVPRSSLSVYDKQVFRAPLVGETAADPKTYGKTLFE
TDAVRAWVDDRAGENDVLIVSFKSKMNTIGPSVIDGLTQAIELAEKDYKGLVVWQPTSLKLGTPGGPFSAGANLEEAMPA
FMMGGAKGIEPFVKKFQQGMLRVKYASVPVISAVSGIALGGGCELALHSAKRVAHIESYLGLVEVGVGLVPAGGGLKEAA
LRAAEAATQVGATNDLLKFVQKSFENAAMAKVSASALDARAMGYLKPSDTIVFNVFELLDVAKKEARALSAAGYRPPLRV
TQVPVAGRSAIATIKASLVNMRDGRFISEHDFLIASRIAEAVCGGDVEAGSLVDEEWLLQLERRAFVDLLGTQKTQERIM
GMLQTGKPVRN

Sequences:

>Translated_811_residues
MSNLIIRKVAVLGAGVMGAQIAAHLINAKVPVLLFDLPAKEGPKNAIALKAIENLKKLSPAPFGVKDDAQYVQPANYDDD
IGKLAECDLVIEAIAERMDWKHDLYRKVAPHLAPNAIFATNTSGLSITALSEGFPDELKARFCGVHFFNPPRYMHLVELI
PTATTRPEILDQLESFLTSVVGKGVVRAKDTPNFIANRVGIFSILAVVTEAAKYGLRFDEVDDLTGARLGRAKSATFRTA
DVVGLDTMAHVIKTMQDTLKDDPFYPVYETPAVLAELVKKGALGQKTGGGFYRKEGKAIKVLDAKTGEYVDGGAKADELV
GRILKRPPAERLKLLRESEHPQAQFLWAIFRDVYHYIGVHLESIADNARDVDLAIRWGFGWNEGPFEGWQTAGWKQVAEW
VQEDIAAGKALSNAPLPSWVLEGPVAEKGGVHTNEGSWSPASKTFVPRSSLSVYDKQVFRAPLVGETAADPKTYGKTLFE
TDAVRAWVDDRAGENDVLIVSFKSKMNTIGPSVIDGLTQAIELAEKDYKGLVVWQPTSLKLGTPGGPFSAGANLEEAMPA
FMMGGAKGIEPFVKKFQQGMLRVKYASVPVISAVSGIALGGGCELALHSAKRVAHIESYLGLVEVGVGLVPAGGGLKEAA
LRAAEAATQVGATNDLLKFVQKSFENAAMAKVSASALDARAMGYLKPSDTIVFNVFELLDVAKKEARALSAAGYRPPLRV
TQVPVAGRSAIATIKASLVNMRDGRFISEHDFLIASRIAEAVCGGDVEAGSLVDEEWLLQLERRAFVDLLGTQKTQERIM
GMLQTGKPVRN
>Mature_810_residues
SNLIIRKVAVLGAGVMGAQIAAHLINAKVPVLLFDLPAKEGPKNAIALKAIENLKKLSPAPFGVKDDAQYVQPANYDDDI
GKLAECDLVIEAIAERMDWKHDLYRKVAPHLAPNAIFATNTSGLSITALSEGFPDELKARFCGVHFFNPPRYMHLVELIP
TATTRPEILDQLESFLTSVVGKGVVRAKDTPNFIANRVGIFSILAVVTEAAKYGLRFDEVDDLTGARLGRAKSATFRTAD
VVGLDTMAHVIKTMQDTLKDDPFYPVYETPAVLAELVKKGALGQKTGGGFYRKEGKAIKVLDAKTGEYVDGGAKADELVG
RILKRPPAERLKLLRESEHPQAQFLWAIFRDVYHYIGVHLESIADNARDVDLAIRWGFGWNEGPFEGWQTAGWKQVAEWV
QEDIAAGKALSNAPLPSWVLEGPVAEKGGVHTNEGSWSPASKTFVPRSSLSVYDKQVFRAPLVGETAADPKTYGKTLFET
DAVRAWVDDRAGENDVLIVSFKSKMNTIGPSVIDGLTQAIELAEKDYKGLVVWQPTSLKLGTPGGPFSAGANLEEAMPAF
MMGGAKGIEPFVKKFQQGMLRVKYASVPVISAVSGIALGGGCELALHSAKRVAHIESYLGLVEVGVGLVPAGGGLKEAAL
RAAEAATQVGATNDLLKFVQKSFENAAMAKVSASALDARAMGYLKPSDTIVFNVFELLDVAKKEARALSAAGYRPPLRVT
QVPVAGRSAIATIKASLVNMRDGRFISEHDFLIASRIAEAVCGGDVEAGSLVDEEWLLQLERRAFVDLLGTQKTQERIMG
MLQTGKPVRN

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG1250

COG function: function code I; 3-hydroxyacyl-CoA dehydrogenase

Gene ontology:

Cell location: Mitochondria or Peroxisomes [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyacyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI296179429, Length=298, Percent_Identity=30.8724832214765, Blast_Score=121, Evalue=3e-27,
Organism=Homo sapiens, GI296179427, Length=313, Percent_Identity=30.3514376996805, Blast_Score=116, Evalue=9e-26,
Organism=Homo sapiens, GI261878539, Length=427, Percent_Identity=24.1217798594848, Blast_Score=90, Evalue=8e-18,
Organism=Homo sapiens, GI68989263, Length=427, Percent_Identity=24.1217798594848, Blast_Score=90, Evalue=9e-18,
Organism=Homo sapiens, GI20127408, Length=420, Percent_Identity=25.952380952381, Blast_Score=88, Evalue=3e-17,
Organism=Escherichia coli, GI1787661, Length=307, Percent_Identity=30.9446254071661, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1790281, Length=229, Percent_Identity=28.3842794759825, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1788682, Length=333, Percent_Identity=25.8258258258258, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17553560, Length=297, Percent_Identity=33.3333333333333, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17563036, Length=295, Percent_Identity=32.5423728813559, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17549919, Length=303, Percent_Identity=33.003300330033, Blast_Score=111, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17558304, Length=390, Percent_Identity=26.6666666666667, Blast_Score=99, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17508951, Length=421, Percent_Identity=27.0783847980998, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17508953, Length=421, Percent_Identity=27.0783847980998, Blast_Score=95, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25144276, Length=295, Percent_Identity=31.5254237288136, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71985923, Length=297, Percent_Identity=26.9360269360269, Blast_Score=83, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71985930, Length=412, Percent_Identity=21.8446601941748, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583077, Length=367, Percent_Identity=23.433242506812, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24583079, Length=367, Percent_Identity=23.433242506812, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19921000, Length=352, Percent_Identity=23.5795454545455, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45549573, Length=226, Percent_Identity=25.2212389380531, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006176
- InterPro:   IPR006108
- InterPro:   IPR008927
- InterPro:   IPR001753
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00725 3HCDH; PF02737 3HCDH_N; PF00378 ECH [H]

EC number: =1.1.1.35 [H]

Molecular weight: Translated: 87558; Mature: 87426

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLIIRKVAVLGAGVMGAQIAAHLINAKVPVLLFDLPAKEGPKNAIALKAIENLKKLSP
CCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCC
APFGVKDDAQYVQPANYDDDIGKLAECDLVIEAIAERMDWKHDLYRKVAPHLAPNAIFAT
CCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEE
NTSGLSITALSEGFPDELKARFCGVHFFNPPRYMHLVELIPTATTRPEILDQLESFLTSV
CCCCEEEEEECCCCCHHHHHHHHCEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VGKGVVRAKDTPNFIANRVGIFSILAVVTEAAKYGLRFDEVDDLTGARLGRAKSATFRTA
HCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCHH
DVVGLDTMAHVIKTMQDTLKDDPFYPVYETPAVLAELVKKGALGQKTGGGFYRKEGKAIK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEECCCCEEE
VLDAKTGEYVDGGAKADELVGRILKRPPAERLKLLRESEHPQAQFLWAIFRDVYHYIGVH
EEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LESIADNARDVDLAIRWGFGWNEGPFEGWQTAGWKQVAEWVQEDIAAGKALSNAPLPSWV
HHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHH
LEGPVAEKGGVHTNEGSWSPASKTFVPRSSLSVYDKQVFRAPLVGETAADPKTYGKTLFE
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
TDAVRAWVDDRAGENDVLIVSFKSKMNTIGPSVIDGLTQAIELAEKDYKGLVVWQPTSLK
HHHHHHHHHCCCCCCCEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEE
LGTPGGPFSAGANLEEAMPAFMMGGAKGIEPFVKKFQQGMLRVKYASVPVISAVSGIALG
ECCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECCCCHHHHHHHHEEC
GGCELALHSAKRVAHIESYLGLVEVGVGLVPAGGGLKEAALRAAEAATQVGATNDLLKFV
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHH
QKSFENAAMAKVSASALDARAMGYLKPSDTIVFNVFELLDVAKKEARALSAAGYRPPLRV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
TQVPVAGRSAIATIKASLVNMRDGRFISEHDFLIASRIAEAVCGGDVEAGSLVDEEWLLQ
EECCCCCCHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LERRAFVDLLGTQKTQERIMGMLQTGKPVRN
HHHHHHHHHHCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SNLIIRKVAVLGAGVMGAQIAAHLINAKVPVLLFDLPAKEGPKNAIALKAIENLKKLSP
CCHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCC
APFGVKDDAQYVQPANYDDDIGKLAECDLVIEAIAERMDWKHDLYRKVAPHLAPNAIFAT
CCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEE
NTSGLSITALSEGFPDELKARFCGVHFFNPPRYMHLVELIPTATTRPEILDQLESFLTSV
CCCCEEEEEECCCCCHHHHHHHHCEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VGKGVVRAKDTPNFIANRVGIFSILAVVTEAAKYGLRFDEVDDLTGARLGRAKSATFRTA
HCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCHH
DVVGLDTMAHVIKTMQDTLKDDPFYPVYETPAVLAELVKKGALGQKTGGGFYRKEGKAIK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEECCCCEEE
VLDAKTGEYVDGGAKADELVGRILKRPPAERLKLLRESEHPQAQFLWAIFRDVYHYIGVH
EEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LESIADNARDVDLAIRWGFGWNEGPFEGWQTAGWKQVAEWVQEDIAAGKALSNAPLPSWV
HHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHH
LEGPVAEKGGVHTNEGSWSPASKTFVPRSSLSVYDKQVFRAPLVGETAADPKTYGKTLFE
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
TDAVRAWVDDRAGENDVLIVSFKSKMNTIGPSVIDGLTQAIELAEKDYKGLVVWQPTSLK
HHHHHHHHHCCCCCCCEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEE
LGTPGGPFSAGANLEEAMPAFMMGGAKGIEPFVKKFQQGMLRVKYASVPVISAVSGIALG
ECCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECCCCHHHHHHHHEEC
GGCELALHSAKRVAHIESYLGLVEVGVGLVPAGGGLKEAALRAAEAATQVGATNDLLKFV
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHH
QKSFENAAMAKVSASALDARAMGYLKPSDTIVFNVFELLDVAKKEARALSAAGYRPPLRV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
TQVPVAGRSAIATIKASLVNMRDGRFISEHDFLIASRIAEAVCGGDVEAGSLVDEEWLLQ
EECCCCCCHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LERRAFVDLLGTQKTQERIMGMLQTGKPVRN
HHHHHHHHHHCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]