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The map label for this gene is clcD [H]

Identifier: 91781516

GI number: 91781516

Start: 158943

End: 159641

Strand: Reverse

Name: clcD [H]

Synonym: Bxe_A4330

Alternate gene names: 91781516

Gene position: 159641-158943 (Counterclockwise)

Preceding gene: 91781521

Following gene: 91781515

Centisome position: 3.26

GC content: 63.81

Gene sequence:

>699_bases
ATGAGCACCACTTCCCGATGGATCGACATTCCCGCCGGTAAAGACAGTTTCGGCGGCTACCTTGCGCTGCCCAAAGGCGG
CAAGGGACCGGCCGTCATCATCATTCAGGAAATCTTCGGCGTGAACAGCCATATCCGCTCGGTCGCGGATCAGTATGCCC
AGGACGGCTACATCGCGCTCGCACCGGACGTGTTCTGGCGGGTGCAGCCGCGCGTGGAATTGACGTATGAAGGCGCGGAT
CGCGAGAAGGGCATCGAGCTGATGCAGAAGACCAACGTCGATCAGGCCGTAGCCGATATCGGCGCAGCCGCCGCCGCGTT
GCGTGCCATGCCGGAAGTGACCGGCAAGATTGCGGCGATCGGCTACTGCTTCGGCGGCCGGCTCGCGTATCTGGCCGCGG
CGCAAGGCACGCTCGACGCAGCGGTGGCCTACTACGGCGGCGGCATCCAGAACCAGCTCGACGAAGCAGCGAAGATCAAG
GTGCCGATGCAATTCCACTACGGCGAGCTCGACGCGCATATTCCGGTGTCGGCTATCGGACAGATTCAGGAGCGCTTCGC
CGGCCGCACCGACGCCGAGTTCCACATCTATCCGAATGCCGATCACGGCTTCAACTGCTCGGACCGCGCGTCGTATAACC
AGCGCGCCGCCGCGCTGGCGCATGGCCGCACGTTGACCTTCCTCGGCGAGCGTCTGTAA

Upstream 100 bases:

>100_bases
CTTTTGATGGCAAACTGACCGCCCCGCTTCAGGTATCCAGGCCTCGGGCATTCATTCAGACCGACCTCGCGCAGCCGCGC
GCGAGTCAATGGAGAGCAAC

Downstream 100 bases:

>100_bases
CTCGCGCCTCTGATTGGCGCGTTGTGTGTGCGGCCATTCGCGCTGCCCGATTGCACGCGCCAATTGCGTGTTTTTATCGG
CGCTTTCTGTTTGCGCCAGC

Product: carboxymethylenebutenolidase

Products: NA

Alternate protein names: Dienelactone hydrolase; DLH [H]

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MSTTSRWIDIPAGKDSFGGYLALPKGGKGPAVIIIQEIFGVNSHIRSVADQYAQDGYIALAPDVFWRVQPRVELTYEGAD
REKGIELMQKTNVDQAVADIGAAAAALRAMPEVTGKIAAIGYCFGGRLAYLAAAQGTLDAAVAYYGGGIQNQLDEAAKIK
VPMQFHYGELDAHIPVSAIGQIQERFAGRTDAEFHIYPNADHGFNCSDRASYNQRAAALAHGRTLTFLGERL

Sequences:

>Translated_232_residues
MSTTSRWIDIPAGKDSFGGYLALPKGGKGPAVIIIQEIFGVNSHIRSVADQYAQDGYIALAPDVFWRVQPRVELTYEGAD
REKGIELMQKTNVDQAVADIGAAAAALRAMPEVTGKIAAIGYCFGGRLAYLAAAQGTLDAAVAYYGGGIQNQLDEAAKIK
VPMQFHYGELDAHIPVSAIGQIQERFAGRTDAEFHIYPNADHGFNCSDRASYNQRAAALAHGRTLTFLGERL
>Mature_231_residues
STTSRWIDIPAGKDSFGGYLALPKGGKGPAVIIIQEIFGVNSHIRSVADQYAQDGYIALAPDVFWRVQPRVELTYEGADR
EKGIELMQKTNVDQAVADIGAAAAALRAMPEVTGKIAAIGYCFGGRLAYLAAAQGTLDAAVAYYGGGIQNQLDEAAKIKV
PMQFHYGELDAHIPVSAIGQIQERFAGRTDAEFHIYPNADHGFNCSDRASYNQRAAALAHGRTLTFLGERL

Specific function: Ring cleavage of cyclic ester dienelactone to produce maleylacetate [H]

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI48994982, Length=234, Percent_Identity=31.1965811965812, Blast_Score=102, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17532655, Length=247, Percent_Identity=29.5546558704453, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: =3.1.1.45 [H]

Molecular weight: Translated: 24962; Mature: 24831

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTSRWIDIPAGKDSFGGYLALPKGGKGPAVIIIQEIFGVNSHIRSVADQYAQDGYIAL
CCCCCCEEECCCCCCCCCCEEEECCCCCCCEEEEEHHHHCCCHHHHHHHHHHHCCCEEEE
APDVFWRVQPRVELTYEGADREKGIELMQKTNVDQAVADIGAAAAALRAMPEVTGKIAAI
CCCEEEEECCCEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEE
GYCFGGRLAYLAAAQGTLDAAVAYYGGGIQNQLDEAAKIKVPMQFHYGELDAHIPVSAIG
EEECCCCEEEEHHCCCCHHHHHHHHCCCHHHHHCCHHEEEEEEEEECCCCCCCCCHHHHH
QIQERFAGRTDAEFHIYPNADHGFNCSDRASYNQRAAALAHGRTLTFLGERL
HHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCC
>Mature Secondary Structure 
STTSRWIDIPAGKDSFGGYLALPKGGKGPAVIIIQEIFGVNSHIRSVADQYAQDGYIAL
CCCCCEEECCCCCCCCCCEEEECCCCCCCEEEEEHHHHCCCHHHHHHHHHHHCCCEEEE
APDVFWRVQPRVELTYEGADREKGIELMQKTNVDQAVADIGAAAAALRAMPEVTGKIAAI
CCCEEEEECCCEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEE
GYCFGGRLAYLAAAQGTLDAAVAYYGGGIQNQLDEAAKIKVPMQFHYGELDAHIPVSAIG
EEECCCCEEEEHHCCCCHHHHHHHHCCCHHHHHCCHHEEEEEEEEECCCCCCCCCHHHHH
QIQERFAGRTDAEFHIYPNADHGFNCSDRASYNQRAAALAHGRTLTFLGERL
HHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3299368 [H]