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The map label for this gene is nylA [H]

Identifier: 91781515

GI number: 91781515

Start: 157153

End: 158721

Strand: Reverse

Name: nylA [H]

Synonym: Bxe_A4331

Alternate gene names: 91781515

Gene position: 158721-157153 (Counterclockwise)

Preceding gene: 91781516

Following gene: 91781510

Centisome position: 3.24

GC content: 66.92

Gene sequence:

>1569_bases
ATGCAGAACCATGCCGATCCAGAGTTCGATACCGAGGGCCCATCCGTCGTGCATTCAGACTATCTCGCCCATGACGCCAC
CGGCCTCGCCGAACTGATACGCAAGCGCGAGGCGAGCGCGCGCGAGTTGCTCGACATAGCGATCTCACGCACCGAAGCCG
TCAATCCCGCGATCAATGCGGTTGTCCTGAAGGACTACGACGCAGCCCGTCTGCGTGCTTCGCGCGACGACGCAAACCGC
GCGGCGAACGGTGCCCACCGTGTGGAGAGCGAAAGCGCGCAAACCGCGCTGGCGGGCGTGCCCTATCTGATCAAGGACCT
CGGCGCACCGGTCGCCGGGCTGCGCATGGCAATGGGCAGCCGCCACTACCGCCATTTCATTCCGGCTCAGGACGCGCCCG
TGGTCACGCTCGTAAAAGCGGCCGGCCTCAACATCTTTGGCAAGACCAGCACGTCCGAGCTCGGCCAGATGCCTTATACG
GAGCCCGAACTGTTTGGTGCGTGCCGCAATCCGTGGAATCTCGACCATACGCCGGGCGGTTCGAGCGGCGGCGCGGCGGC
GGCGGTTGCCGCGGGCATCGTGCCGCTCGCCCATGCGTCGGACGGCGGTGGCTCGATCCGCATTCCGGCCTCATGCTGCG
GACTCTTCGGCCTCAAACCTTCACGCGGGCGCGTGCCGCGCGCGACAGCGGCGGCTGCCGGCGAACTCGGCGTCGATCAC
GCGGTATCGCGCAGCGTGCGCGACAGTGCGTTGCTGCTCGATCTGCTCGCGGGCAATGCGGACAGACCGCCCGGCGCACC
AGGCACGTTTCTGAGCGCGAGCCGCGAGCCGTGCAAGCCGCTGAACATCGCGTACGTCACCGAGCCGATGCTGGCGCCCT
CGCTTTCCGCCGACTCGCGCGCCGCGCTGGACGACGCCGCGCAACTCGCCAGTTCGCTCGGCCATAACATCGAACCGGTG
TCGCTCGGCATCGACTTCGCGGCGGTCCGGCATGCGTTCCTCACGCTCTGGTCCGTGACAGCGGAAGATCTCGTGCTCAA
CGCCGAGCGCATCAGCGGCCGCAAACCGCTGCGTGCCGAATTCGAAATTTCGACGTGGGCGATGGCGCACGTGGGCCGCA
AACTCGGCGAGCGTGGCCTGCCCGCCGCGCTCGAAGAACAGCGCCGTATCACCGAACGACTCACTGATCTGCTGAATCGC
TACGACGTGATTCTGTGCGCCACGCTGGCCGCGCCGCCGATCAAGATCGGCGAGATGCAGCCCACCTCTGCTGAACGCAT
GCAGATGCGCGCCGTCACCGCCATGCCGCTCGAAGCGCTGATGAAGAAGGTGCTGGCGGAAGCGTCGAACAAGGCATTCA
CGTGGGCCGGTTGCACGGAGCTGTTCAATCTGAGCGGTCAGCCGGCGATGTCGGTACCGCTTTACTGGACTGCGCGCGGG
CTGCCGGTGGGCGTGCAGTTTGCCGCGCGCGAGGGCGGCGAGGCCATGCTTCTGCGTCTGGCCACGCAACTCGAAACGGC
CCGGCCGTGGTTCGACAGGCGGCCGCCGTTGATGCAGGCACGCAACTGA

Upstream 100 bases:

>100_bases
TACTCTCTCAAAGACGGCGTGTCAGCAGCACGCCGTCTTCTTCCGTGTGGGGCAATCAGCCCGGGCGGCGTGAGGTCCGG
CAGAACGGTTCACCGCGCGC

Downstream 100 bases:

>100_bases
ACGAAGACGCCGCATGCGGCGGAGAGAGTGGCAAAGACGGCGAAGGTGATGACGGCAGCAAGGCAGCAAAGGCACCGAAA
GCATCCAAGGCACCAAAGGC

Product: amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MQNHADPEFDTEGPSVVHSDYLAHDATGLAELIRKREASARELLDIAISRTEAVNPAINAVVLKDYDAARLRASRDDANR
AANGAHRVESESAQTALAGVPYLIKDLGAPVAGLRMAMGSRHYRHFIPAQDAPVVTLVKAAGLNIFGKTSTSELGQMPYT
EPELFGACRNPWNLDHTPGGSSGGAAAAVAAGIVPLAHASDGGGSIRIPASCCGLFGLKPSRGRVPRATAAAAGELGVDH
AVSRSVRDSALLLDLLAGNADRPPGAPGTFLSASREPCKPLNIAYVTEPMLAPSLSADSRAALDDAAQLASSLGHNIEPV
SLGIDFAAVRHAFLTLWSVTAEDLVLNAERISGRKPLRAEFEISTWAMAHVGRKLGERGLPAALEEQRRITERLTDLLNR
YDVILCATLAAPPIKIGEMQPTSAERMQMRAVTAMPLEALMKKVLAEASNKAFTWAGCTELFNLSGQPAMSVPLYWTARG
LPVGVQFAAREGGEAMLLRLATQLETARPWFDRRPPLMQARN

Sequences:

>Translated_522_residues
MQNHADPEFDTEGPSVVHSDYLAHDATGLAELIRKREASARELLDIAISRTEAVNPAINAVVLKDYDAARLRASRDDANR
AANGAHRVESESAQTALAGVPYLIKDLGAPVAGLRMAMGSRHYRHFIPAQDAPVVTLVKAAGLNIFGKTSTSELGQMPYT
EPELFGACRNPWNLDHTPGGSSGGAAAAVAAGIVPLAHASDGGGSIRIPASCCGLFGLKPSRGRVPRATAAAAGELGVDH
AVSRSVRDSALLLDLLAGNADRPPGAPGTFLSASREPCKPLNIAYVTEPMLAPSLSADSRAALDDAAQLASSLGHNIEPV
SLGIDFAAVRHAFLTLWSVTAEDLVLNAERISGRKPLRAEFEISTWAMAHVGRKLGERGLPAALEEQRRITERLTDLLNR
YDVILCATLAAPPIKIGEMQPTSAERMQMRAVTAMPLEALMKKVLAEASNKAFTWAGCTELFNLSGQPAMSVPLYWTARG
LPVGVQFAAREGGEAMLLRLATQLETARPWFDRRPPLMQARN
>Mature_522_residues
MQNHADPEFDTEGPSVVHSDYLAHDATGLAELIRKREASARELLDIAISRTEAVNPAINAVVLKDYDAARLRASRDDANR
AANGAHRVESESAQTALAGVPYLIKDLGAPVAGLRMAMGSRHYRHFIPAQDAPVVTLVKAAGLNIFGKTSTSELGQMPYT
EPELFGACRNPWNLDHTPGGSSGGAAAAVAAGIVPLAHASDGGGSIRIPASCCGLFGLKPSRGRVPRATAAAAGELGVDH
AVSRSVRDSALLLDLLAGNADRPPGAPGTFLSASREPCKPLNIAYVTEPMLAPSLSADSRAALDDAAQLASSLGHNIEPV
SLGIDFAAVRHAFLTLWSVTAEDLVLNAERISGRKPLRAEFEISTWAMAHVGRKLGERGLPAALEEQRRITERLTDLLNR
YDVILCATLAAPPIKIGEMQPTSAERMQMRAVTAMPLEALMKKVLAEASNKAFTWAGCTELFNLSGQPAMSVPLYWTARG
LPVGVQFAAREGGEAMLLRLATQLETARPWFDRRPPLMQARN

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=536, Percent_Identity=26.6791044776119, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI222831590, Length=540, Percent_Identity=25, Blast_Score=95, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17537465, Length=325, Percent_Identity=28.9230769230769, Blast_Score=102, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17556264, Length=327, Percent_Identity=28.7461773700306, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=394, Percent_Identity=26.1421319796954, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=321, Percent_Identity=26.1682242990654, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=321, Percent_Identity=26.1682242990654, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI32565337, Length=179, Percent_Identity=30.7262569832402, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320448, Length=213, Percent_Identity=30.0469483568075, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45550774, Length=325, Percent_Identity=32, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24648435, Length=337, Percent_Identity=31.7507418397626, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24648437, Length=337, Percent_Identity=31.7507418397626, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24644968, Length=256, Percent_Identity=32.421875, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI161078093, Length=534, Percent_Identity=25.6554307116105, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21356731, Length=249, Percent_Identity=31.3253012048193, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24652985, Length=258, Percent_Identity=31.7829457364341, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19922090, Length=258, Percent_Identity=31.7829457364341, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24652981, Length=258, Percent_Identity=31.7829457364341, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24652983, Length=258, Percent_Identity=31.7829457364341, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648113, Length=462, Percent_Identity=28.7878787878788, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648441, Length=180, Percent_Identity=35, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648439, Length=180, Percent_Identity=35, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 55568; Mature: 55568

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNHADPEFDTEGPSVVHSDYLAHDATGLAELIRKREASARELLDIAISRTEAVNPAINA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VVLKDYDAARLRASRDDANRAANGAHRVESESAQTALAGVPYLIKDLGAPVAGLRMAMGS
EEEECCCHHHHHCCCCHHHHHCCCHHHHCCHHHHHHHHCCHHHHHHHCCCHHHHHHHHCC
RHYRHFIPAQDAPVVTLVKAAGLNIFGKTSTSELGQMPYTEPELFGACRNPWNLDHTPGG
CHHHCCCCCCCCCCEEEEHHCCCEEECCCCHHHHCCCCCCCHHHHHHCCCCCCCCCCCCC
SSGGAAAAVAAGIVPLAHASDGGGSIRIPASCCGLFGLKPSRGRVPRATAAAAGELGVDH
CCCCHHHHHHHHHHHEEECCCCCCEEEECHHHHHHCCCCCCCCCCCCHHHHHHCCCCHHH
AVSRSVRDSALLLDLLAGNADRPPGAPGTFLSASREPCKPLNIAYVTEPMLAPSLSADSR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCH
AALDDAAQLASSLGHNIEPVSLGIDFAAVRHAFLTLWSVTAEDLVLNAERISGRKPLRAE
HHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHEEHHHHCCCCCCCEE
FEISTWAMAHVGRKLGERGLPAALEEQRRITERLTDLLNRYDVILCATLAAPPIKIGEMQ
EEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEHHHCCCCCCCCCCC
PTSAERMQMRAVTAMPLEALMKKVLAEASNKAFTWAGCTELFNLSGQPAMSVPLYWTARG
CCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECHHHHHCCCCCCCEECCEEEEECC
LPVGVQFAAREGGEAMLLRLATQLETARPWFDRRPPLMQARN
CCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQNHADPEFDTEGPSVVHSDYLAHDATGLAELIRKREASARELLDIAISRTEAVNPAINA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VVLKDYDAARLRASRDDANRAANGAHRVESESAQTALAGVPYLIKDLGAPVAGLRMAMGS
EEEECCCHHHHHCCCCHHHHHCCCHHHHCCHHHHHHHHCCHHHHHHHCCCHHHHHHHHCC
RHYRHFIPAQDAPVVTLVKAAGLNIFGKTSTSELGQMPYTEPELFGACRNPWNLDHTPGG
CHHHCCCCCCCCCCEEEEHHCCCEEECCCCHHHHCCCCCCCHHHHHHCCCCCCCCCCCCC
SSGGAAAAVAAGIVPLAHASDGGGSIRIPASCCGLFGLKPSRGRVPRATAAAAGELGVDH
CCCCHHHHHHHHHHHEEECCCCCCEEEECHHHHHHCCCCCCCCCCCCHHHHHHCCCCHHH
AVSRSVRDSALLLDLLAGNADRPPGAPGTFLSASREPCKPLNIAYVTEPMLAPSLSADSR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCH
AALDDAAQLASSLGHNIEPVSLGIDFAAVRHAFLTLWSVTAEDLVLNAERISGRKPLRAE
HHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHEEHHHHCCCCCCCEE
FEISTWAMAHVGRKLGERGLPAALEEQRRITERLTDLLNRYDVILCATLAAPPIKIGEMQ
EEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEHHHCCCCCCCCCCC
PTSAERMQMRAVTAMPLEALMKKVLAEASNKAFTWAGCTELFNLSGQPAMSVPLYWTARG
CCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECHHHHHCCCCCCCEECCEEEEECC
LPVGVQFAAREGGEAMLLRLATQLETARPWFDRRPPLMQARN
CCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]