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The map label for this gene is ooxB [H]

Identifier: 91790208

GI number: 91790208

Start: 4614847

End: 4616004

Strand: Reverse

Name: ooxB [H]

Synonym: Bpro_4373

Alternate gene names: 91790208

Gene position: 4616004-4614847 (Counterclockwise)

Preceding gene: 91790209

Following gene: 91790207

Centisome position: 88.76

GC content: 68.31

Gene sequence:

>1158_bases
ATGAGGCCTGATGGCACCGGGCTGCCGCTGGGCAGCGACGTCATCGTCATAGGTGGCGGTTTCCACGGCACCTCCAGCGC
ATTGCACCTTTCGCGCCGCGGCGCGAAGGTCACGTTGCTGGAAGCCGAGTACTGCGCGCGCCATGCTTCGGGCGTCAATG
CGGGTGGTGTGCGAAGCCTGGGTCGCCACTATGCCGAGGTGCCCCTGGCGCGCGCCTCGCTTGGCCTGTGGCACAGCTTG
CCCGAGCTTATCGGTAAAGACCTGGGCGACGACGCGGCCTTCGTGGCCAGCGGCATGCTGCAGATCGCCGAAACACCGCA
GGAACTGGACAAGCTCCGCCGCCGCGTGGCCGAGCTGAACGCGCTCGGCTTCACCCACGAAGTCATCGTCGACGCGCAGC
AGGTGCGCGAGATCGCGCCGCGACTGGCGCATCACGTGGTCGGCGGCATCTGGGTTAAGGACGACGGCCATGCCGTGCCT
TACCGCGCCGTCACGGCCTTTCGCCATGCCGCGCAGCGGCTGGGCGCGCAGTTTCATGAAGCCACGCCTGCGGAGACGAT
CGAACGCGTGGGCAGCCAATGGCACGTCACCACGCCGCGCGGCGTCTTCACCGCGCCGTGGCTGGTGAATGCGGCTGGCG
CCTGGTCTGGCGACTTCGCCGCGCAGGCGGGCGACGTGGTGCCGATGAAGCCCGGCGGGCTGATGCTGATGATCACGCAG
CGTGTGCCGCACTTCGTCGATCCGGTGCTGAGCGCGGCCGGCCGTCCGCTGTCCTTCAAGCAGTTCGCCAACGGCACCGT
GCTCATAGGCGGCGGCCTGCGCTGCGCGGCCGACGTGGTGCGCCGCCATGGCGAGATCGACTTCCTCAAGCTTTCGTCGA
GCGCACAGACCGTGACCGACCTGTTCCCGCACCTGGCCGGCATTAACGTCGTGCGCGCCTGGGCCGGCGTCGAGGCCTTT
ATGCCCGACGAGATCCCCGTCATCAGCCTGAGCCGCCATGCGCCTCAACTGGTGCACGCTTTCGGTTTCTCGGCCCACGG
CTTCGAGCTCGGTCCCATCGGCGGGCAGATCGTTTCCGAACTGGTGTTCGATGGGCGCAGCACCTTGCCGATCGCGCCGT
TTGCCGTGGACCGGTTCGCCCCGGCCGCCGTGCATTGA

Upstream 100 bases:

>100_bases
ACCGAGGTGATCGCCGATGCGCGCGGCGTGTCGCCCGGCGAGACCGGCTACTTTCGCATACGTCCGCCGATCAAGCCGAT
CACACTTGGAGAGCTTGCGC

Downstream 100 bases:

>100_bases
CCGGCCCGCTCCTTGCCTTCCCTTTTTCCTTCTCACCAACCACACAGAAAGACCACTCAATGTCTGACATCCAGCGCTAC
GAATCCACGCCCCGCCTGTC

Product: FAD dependent oxidoreductase

Products: glycine; formaldehyde; H2O2

Alternate protein names: Octopine oxidase subunit B [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MRPDGTGLPLGSDVIVIGGGFHGTSSALHLSRRGAKVTLLEAEYCARHASGVNAGGVRSLGRHYAEVPLARASLGLWHSL
PELIGKDLGDDAAFVASGMLQIAETPQELDKLRRRVAELNALGFTHEVIVDAQQVREIAPRLAHHVVGGIWVKDDGHAVP
YRAVTAFRHAAQRLGAQFHEATPAETIERVGSQWHVTTPRGVFTAPWLVNAAGAWSGDFAAQAGDVVPMKPGGLMLMITQ
RVPHFVDPVLSAAGRPLSFKQFANGTVLIGGGLRCAADVVRRHGEIDFLKLSSSAQTVTDLFPHLAGINVVRAWAGVEAF
MPDEIPVISLSRHAPQLVHAFGFSAHGFELGPIGGQIVSELVFDGRSTLPIAPFAVDRFAPAAVH

Sequences:

>Translated_385_residues
MRPDGTGLPLGSDVIVIGGGFHGTSSALHLSRRGAKVTLLEAEYCARHASGVNAGGVRSLGRHYAEVPLARASLGLWHSL
PELIGKDLGDDAAFVASGMLQIAETPQELDKLRRRVAELNALGFTHEVIVDAQQVREIAPRLAHHVVGGIWVKDDGHAVP
YRAVTAFRHAAQRLGAQFHEATPAETIERVGSQWHVTTPRGVFTAPWLVNAAGAWSGDFAAQAGDVVPMKPGGLMLMITQ
RVPHFVDPVLSAAGRPLSFKQFANGTVLIGGGLRCAADVVRRHGEIDFLKLSSSAQTVTDLFPHLAGINVVRAWAGVEAF
MPDEIPVISLSRHAPQLVHAFGFSAHGFELGPIGGQIVSELVFDGRSTLPIAPFAVDRFAPAAVH
>Mature_385_residues
MRPDGTGLPLGSDVIVIGGGFHGTSSALHLSRRGAKVTLLEAEYCARHASGVNAGGVRSLGRHYAEVPLARASLGLWHSL
PELIGKDLGDDAAFVASGMLQIAETPQELDKLRRRVAELNALGFTHEVIVDAQQVREIAPRLAHHVVGGIWVKDDGHAVP
YRAVTAFRHAAQRLGAQFHEATPAETIERVGSQWHVTTPRGVFTAPWLVNAAGAWSGDFAAQAGDVVPMKPGGLMLMITQ
RVPHFVDPVLSAAGRPLSFKQFANGTVLIGGGLRCAADVVRRHGEIDFLKLSSSAQTVTDLFPHLAGINVVRAWAGVEAF
MPDEIPVISLSRHAPQLVHAFGFSAHGFELGPIGGQIVSELVFDGRSTLPIAPFAVDRFAPAAVH

Specific function: Oxidative cleavage of octopine into L-arginine and pyruvate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI24797151, Length=398, Percent_Identity=22.8643216080402, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI197927446, Length=395, Percent_Identity=25.5696202531646, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI21361378, Length=395, Percent_Identity=25.5696202531646, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI194306651, Length=396, Percent_Identity=24.4949494949495, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1787559, Length=400, Percent_Identity=23.5, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787438, Length=289, Percent_Identity=23.1833910034602, Blast_Score=64, Evalue=1e-11,
Organism=Drosophila melanogaster, GI20130091, Length=389, Percent_Identity=26.2210796915167, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI28571104, Length=390, Percent_Identity=22.5641025641026, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.5.3.1

Molecular weight: Translated: 40947; Mature: 40947

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPDGTGLPLGSDVIVIGGGFHGTSSALHLSRRGAKVTLLEAEYCARHASGVNAGGVRSL
CCCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCEEEEEEHHHHHHHHCCCCCCHHHHH
GRHYAEVPLARASLGLWHSLPELIGKDLGDDAAFVASGMLQIAETPQELDKLRRRVAELN
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ALGFTHEVIVDAQQVREIAPRLAHHVVGGIWVKDDGHAVPYRAVTAFRHAAQRLGAQFHE
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHC
ATPAETIERVGSQWHVTTPRGVFTAPWLVNAAGAWSGDFAAQAGDVVPMKPGGLMLMITQ
CCCHHHHHHCCCCEEEECCCCCEECCHHEECCCCCCCCHHHCCCCEEEECCCCEEEEEHH
RVPHFVDPVLSAAGRPLSFKQFANGTVLIGGGLRCAADVVRRHGEIDFLKLSSSAQTVTD
HCCHHHHHHHHHCCCCCCHHHHCCCEEEEECCHHHHHHHHHHCCCEEEEEECCCHHHHHH
LFPHLAGINVVRAWAGVEAFMPDEIPVISLSRHAPQLVHAFGFSAHGFELGPIGGQIVSE
HHHHHHHHHHHHHHHCCHHCCCCCCCEEEECCCCHHHHHHHCCCCCCEEECCCCHHHHHH
LVFDGRSTLPIAPFAVDRFAPAAVH
HHHCCCCCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MRPDGTGLPLGSDVIVIGGGFHGTSSALHLSRRGAKVTLLEAEYCARHASGVNAGGVRSL
CCCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCEEEEEEHHHHHHHHCCCCCCHHHHH
GRHYAEVPLARASLGLWHSLPELIGKDLGDDAAFVASGMLQIAETPQELDKLRRRVAELN
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ALGFTHEVIVDAQQVREIAPRLAHHVVGGIWVKDDGHAVPYRAVTAFRHAAQRLGAQFHE
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHC
ATPAETIERVGSQWHVTTPRGVFTAPWLVNAAGAWSGDFAAQAGDVVPMKPGGLMLMITQ
CCCHHHHHHCCCCEEEECCCCCEECCHHEECCCCCCCCHHHCCCCEEEECCCCEEEEEHH
RVPHFVDPVLSAAGRPLSFKQFANGTVLIGGGLRCAADVVRRHGEIDFLKLSSSAQTVTD
HCCHHHHHHHHHCCCCCCHHHHCCCEEEEECCHHHHHHHHHHCCCEEEEEECCCHHHHHH
LFPHLAGINVVRAWAGVEAFMPDEIPVISLSRHAPQLVHAFGFSAHGFELGPIGGQIVSE
HHHHHHHHHHHHHHHCCHHCCCCCCCEEEECCCCHHHHHHHCCCCCCEEECCCCHHHHHH
LVFDGRSTLPIAPFAVDRFAPAAVH
HHHCCCCCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sarcosine; H2O; O2

Specific reaction: sarcosine + H2O + O2 = glycine + formaldehyde + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA