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The map label for this gene is braD [H]

Identifier: 91789917

GI number: 91789917

Start: 4288257

End: 4289141

Strand: Direct

Name: braD [H]

Synonym: Bpro_4078

Alternate gene names: 91789917

Gene position: 4288257-4289141 (Clockwise)

Preceding gene: 91789916

Following gene: 91789918

Centisome position: 82.46

GC content: 61.02

Gene sequence:

>885_bases
ATGGAAATTTTCGGTATTCCCCATCAAGCTTTTCTGGGCCAGCTGCTGCTGGGCCTGGTCAATGGCGCGTTTTACGCCAT
GCTCAGCCTCGGGCTGGCAGTCATCTTTGGCCTGCTGGGCATCGTGAACTTCGCCCACGGAGCGCTCTACATGCTGGGCG
CTTTTGCGGCCTGGATCATGCTCGACAAATTCGGCCTCAACTACTGGGCTGCACTGGTGTTGGCCCCGCTGACAGTCGGC
GCGCTCGGCGTGGTGATTGAACGCCTGTTCCTCAAGCATCTGTACAAGCTGGACCCGCTCTATGGGCTGCTGCTGACCTT
TGGCCTGGCGCTTATCGCGGAAGGCATTTTTCGCGAGCTGTATGGCGTGTCAGGCCAGAATTACAACGTGCCGGAACTGC
TGTCGGGGGCCACCAACCTCGGCTTCATGACTCTGCCGAATTACCGGGCCTGGGTGGTGCTGGTGTCGCTGGTGGTTTGC
CTGGGAACCTGGTTCCTGATTGAACGCACACGGCTGGGCGCCTACCTGCGCGCCGGCACGGAGAACGCAGCACTGGTGCA
GGCCTTCGGCATCAATGTGCCGATGATGGTGATGCTGACCTATGGCGCCGGCGCGGCGCTGGCGGCGCTGGCCGGCGTGC
TGGCCGCGCCCATCATTCAGGTCAATCCGCTAATGGGCTCCAACCTGATCATCGTGGTGTTTGCCGTGGTGGTGATCGGC
GGCATGGGCTCGATTCTGGGCTCCGTCATTACCGGCCTGGCCCTCGGCCTGGTTGAAGGCATGACCCGGGTGTTTTACCC
TGAAGCGTCCAATATTGTGGTGTTTGTGGTCATGGTGCTGGTGCTGATGGTTCGCCCATCGGGCCTGTTTGGCAAGGAGG
CATAG

Upstream 100 bases:

>100_bases
CAAGACCGCATGAACACACCTTTATTCGCTCACGCACGAGTTCACCTTTCGGGTGGCAGCGCGTCCAACCGCCATTTCTA
GGCTCAACTGACGGGCCTCC

Downstream 100 bases:

>100_bases
GTGTTGGCCCCCACGCTTGTCGCTTCGCGTACTGCGCTGCCCCCCGAGGGGGCCGCTGCGCCTGCGGTCTGGCAAAGCCA
GTCCCGCGGCCCCTGCCGGG

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MEIFGIPHQAFLGQLLLGLVNGAFYAMLSLGLAVIFGLLGIVNFAHGALYMLGAFAAWIMLDKFGLNYWAALVLAPLTVG
ALGVVIERLFLKHLYKLDPLYGLLLTFGLALIAEGIFRELYGVSGQNYNVPELLSGATNLGFMTLPNYRAWVVLVSLVVC
LGTWFLIERTRLGAYLRAGTENAALVQAFGINVPMMVMLTYGAGAALAALAGVLAAPIIQVNPLMGSNLIIVVFAVVVIG
GMGSILGSVITGLALGLVEGMTRVFYPEASNIVVFVVMVLVLMVRPSGLFGKEA

Sequences:

>Translated_294_residues
MEIFGIPHQAFLGQLLLGLVNGAFYAMLSLGLAVIFGLLGIVNFAHGALYMLGAFAAWIMLDKFGLNYWAALVLAPLTVG
ALGVVIERLFLKHLYKLDPLYGLLLTFGLALIAEGIFRELYGVSGQNYNVPELLSGATNLGFMTLPNYRAWVVLVSLVVC
LGTWFLIERTRLGAYLRAGTENAALVQAFGINVPMMVMLTYGAGAALAALAGVLAAPIIQVNPLMGSNLIIVVFAVVVIG
GMGSILGSVITGLALGLVEGMTRVFYPEASNIVVFVVMVLVLMVRPSGLFGKEA
>Mature_294_residues
MEIFGIPHQAFLGQLLLGLVNGAFYAMLSLGLAVIFGLLGIVNFAHGALYMLGAFAAWIMLDKFGLNYWAALVLAPLTVG
ALGVVIERLFLKHLYKLDPLYGLLLTFGLALIAEGIFRELYGVSGQNYNVPELLSGATNLGFMTLPNYRAWVVLVSLVVC
LGTWFLIERTRLGAYLRAGTENAALVQAFGINVPMMVMLTYGAGAALAALAGVLAAPIIQVNPLMGSNLIIVVFAVVVIG
GMGSILGSVITGLALGLVEGMTRVFYPEASNIVVFVVMVLVLMVRPSGLFGKEA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=297, Percent_Identity=29.2929292929293, Blast_Score=101, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 31215; Mature: 31215

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIFGIPHQAFLGQLLLGLVNGAFYAMLSLGLAVIFGLLGIVNFAHGALYMLGAFAAWIM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDKFGLNYWAALVLAPLTVGALGVVIERLFLKHLYKLDPLYGLLLTFGLALIAEGIFREL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
YGVSGQNYNVPELLSGATNLGFMTLPNYRAWVVLVSLVVCLGTWFLIERTRLGAYLRAGT
HCCCCCCCCCHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ENAALVQAFGINVPMMVMLTYGAGAALAALAGVLAAPIIQVNPLMGSNLIIVVFAVVVIG
CCHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
GMGSILGSVITGLALGLVEGMTRVFYPEASNIVVFVVMVLVLMVRPSGLFGKEA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MEIFGIPHQAFLGQLLLGLVNGAFYAMLSLGLAVIFGLLGIVNFAHGALYMLGAFAAWIM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDKFGLNYWAALVLAPLTVGALGVVIERLFLKHLYKLDPLYGLLLTFGLALIAEGIFREL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
YGVSGQNYNVPELLSGATNLGFMTLPNYRAWVVLVSLVVCLGTWFLIERTRLGAYLRAGT
HCCCCCCCCCHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ENAALVQAFGINVPMMVMLTYGAGAALAALAGVLAAPIIQVNPLMGSNLIIVVFAVVVIG
CCHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
GMGSILGSVITGLALGLVEGMTRVFYPEASNIVVFVVMVLVLMVRPSGLFGKEA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]