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The map label for this gene is cqsA [H]

Identifier: 91789887

GI number: 91789887

Start: 4249528

End: 4250781

Strand: Reverse

Name: cqsA [H]

Synonym: Bpro_4048

Alternate gene names: 91789887

Gene position: 4250781-4249528 (Counterclockwise)

Preceding gene: 91789888

Following gene: 91789884

Centisome position: 81.74

GC content: 65.31

Gene sequence:

>1254_bases
ATGCAAGTTCTTGAACGCAAGCCAGACCTTTCCCCCAAGATCTATCGTGAACCACCGCTGCTGCCGGGCCTGCGCGAGCG
TCTGGACAAGCAGTTCATGCCGCACTGGAACACTCAGTGGGGCGGCAAGTGCATTCTGCACGGACGCAACCCGGGACCCG
ACTCCATTCGGCTCGATGGCAACGATTACCTCAACATCTCCGGGCATCCCGAGATCGTCAGCGCACAGATTGCCACTTTG
CGCCGCGACAATGACAGCGTGATCCAGTCGGGCGCCTTCCTGCTCGATGCGCATCCTTCCCGCCGGCTCGAGGCCTCCAT
GGCCGCGTGGATTGGCAAGGAGGATGGCTTCATCTGCCAGTCCGGCTATGCCGCCAACGTCGGTTTGCTGCAGGCCATTG
CCGACGAGAAGACGCCGGTGTATCTCGACACCCTGGCCCATACCTCGCTCTGGGAGGGTGTGCGTGCCGCGCGTGCGCCG
GCCCATCCGTTTCGTCACAACGACCCGGCCCACCTCTCACGCATGATTGCCCGCAACGGGCCTGGCGTGGTGGTGGTGGA
TTCTGTGTACAGCACCACCGGCGCCCTGTGCCCGCTGGTGGAGATGGTGGAGGTGGCCGAGCAGCACGGCTGCACCATCC
TGGTTGATGAGTCGCATTCGCTGGGCACCCATGGCCCGCAGGGGGCCGGCCTGTGCGCCGAGCTCGGGCTGGTAGACCGC
GTGCATTTCATCACCGCCAGCCTGGCCAAGGCCTTTGCGGGGCGGGCCGGCTTCTTCACGGTGCCGGCCGAGTTGCGCTA
CTACGTGCTGCACCACAGCTATCCCAATATCTTCAGCTCCTGCCTGCTGCCCCATGAAATCGCTGGACTGGCGGCCACGC
TGGATGTCATTCGCGCCAGCGACGAGGCGCGTGAGCGCTTGCATATCAACACGCAGCGCCTGCGTGCCAGCCTGTCGGAC
ATGGGTTACCCGATTCACCAGGGCAGCCAGCAGATCATTTCGCTGGAGGCCGGGACCGAGCCGGACACCATGGTGCTGCG
CGACCGGCTCGAGGAGCGCCAGCTGTTCGGTGCCATCTTCTGTGCGCCGGCCACCAGCCGCAACCGCGCCATGGTCAGGC
TGACGCTCAATGCCGGCCTGACAGCCTCGGAGCTGGACCGGATCGAGAAGGTAGCGCGGGAAGTGGCGCCGCTCGTCAAA
CCCTGGGACTGGCCGATTGCGCGGCGTGCGCGCGCCGGCCAGGCTGAAATCTAG

Upstream 100 bases:

>100_bases
GGTCAGTCTGGGGCCGCACCCTGTCAGTTCTGACAGGGTAGAGGGCGTATCAATTTGTGATAGCGCTTTCAATGGGGTTT
CTTTACCAAGGAGCGACCCC

Downstream 100 bases:

>100_bases
TACTCCGACCCAGAAATATCGAAACATGAAAGCGCGCCTTCGAACCCATGCCGTCGTTGCAAATCCTCGCCATAGCTACG
GCTATGTCTGCGGTTTGCGC

Product: hypothetical protein

Products: 8-Amino-7-oxononanoate; CO2; Coenzyme A [C]

Alternate protein names: Cholerae quorum-sensing autoinducer [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MQVLERKPDLSPKIYREPPLLPGLRERLDKQFMPHWNTQWGGKCILHGRNPGPDSIRLDGNDYLNISGHPEIVSAQIATL
RRDNDSVIQSGAFLLDAHPSRRLEASMAAWIGKEDGFICQSGYAANVGLLQAIADEKTPVYLDTLAHTSLWEGVRAARAP
AHPFRHNDPAHLSRMIARNGPGVVVVDSVYSTTGALCPLVEMVEVAEQHGCTILVDESHSLGTHGPQGAGLCAELGLVDR
VHFITASLAKAFAGRAGFFTVPAELRYYVLHHSYPNIFSSCLLPHEIAGLAATLDVIRASDEARERLHINTQRLRASLSD
MGYPIHQGSQQIISLEAGTEPDTMVLRDRLEERQLFGAIFCAPATSRNRAMVRLTLNAGLTASELDRIEKVAREVAPLVK
PWDWPIARRARAGQAEI

Sequences:

>Translated_417_residues
MQVLERKPDLSPKIYREPPLLPGLRERLDKQFMPHWNTQWGGKCILHGRNPGPDSIRLDGNDYLNISGHPEIVSAQIATL
RRDNDSVIQSGAFLLDAHPSRRLEASMAAWIGKEDGFICQSGYAANVGLLQAIADEKTPVYLDTLAHTSLWEGVRAARAP
AHPFRHNDPAHLSRMIARNGPGVVVVDSVYSTTGALCPLVEMVEVAEQHGCTILVDESHSLGTHGPQGAGLCAELGLVDR
VHFITASLAKAFAGRAGFFTVPAELRYYVLHHSYPNIFSSCLLPHEIAGLAATLDVIRASDEARERLHINTQRLRASLSD
MGYPIHQGSQQIISLEAGTEPDTMVLRDRLEERQLFGAIFCAPATSRNRAMVRLTLNAGLTASELDRIEKVAREVAPLVK
PWDWPIARRARAGQAEI
>Mature_417_residues
MQVLERKPDLSPKIYREPPLLPGLRERLDKQFMPHWNTQWGGKCILHGRNPGPDSIRLDGNDYLNISGHPEIVSAQIATL
RRDNDSVIQSGAFLLDAHPSRRLEASMAAWIGKEDGFICQSGYAANVGLLQAIADEKTPVYLDTLAHTSLWEGVRAARAP
AHPFRHNDPAHLSRMIARNGPGVVVVDSVYSTTGALCPLVEMVEVAEQHGCTILVDESHSLGTHGPQGAGLCAELGLVDR
VHFITASLAKAFAGRAGFFTVPAELRYYVLHHSYPNIFSSCLLPHEIAGLAATLDVIRASDEARERLHINTQRLRASLSD
MGYPIHQGSQQIISLEAGTEPDTMVLRDRLEERQLFGAIFCAPATSRNRAMVRLTLNAGLTASELDRIEKVAREVAPLVK
PWDWPIARRARAGQAEI

Specific function: Required for the synthesis of the quorum-sensing autoinducer CAI-1 ((S)-3-hydroxytridecan-4-one) which probably functions as an intragenus signal [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI284448556, Length=361, Percent_Identity=27.4238227146814, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI7657118, Length=374, Percent_Identity=28.0748663101604, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI83977444, Length=322, Percent_Identity=27.9503105590062, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI83977442, Length=322, Percent_Identity=27.9503105590062, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI83977440, Length=322, Percent_Identity=27.9503105590062, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI4502025, Length=328, Percent_Identity=27.4390243902439, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI40316939, Length=328, Percent_Identity=27.4390243902439, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI4758668, Length=326, Percent_Identity=24.5398773006135, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI119220554, Length=414, Percent_Identity=21.7391304347826, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI5454084, Length=223, Percent_Identity=26.457399103139, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI1786993, Length=342, Percent_Identity=27.7777777777778, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1790046, Length=343, Percent_Identity=26.2390670553936, Blast_Score=133, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71994529, Length=342, Percent_Identity=24.5614035087719, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI32566772, Length=400, Percent_Identity=22.5, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17560912, Length=307, Percent_Identity=22.8013029315961, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17560914, Length=307, Percent_Identity=22.8013029315961, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71982617, Length=282, Percent_Identity=23.758865248227, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71982625, Length=284, Percent_Identity=24.2957746478873, Blast_Score=81, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6320438, Length=416, Percent_Identity=24.2788461538462, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6320267, Length=315, Percent_Identity=26.3492063492064, Blast_Score=119, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6323954, Length=280, Percent_Identity=20, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24662918, Length=346, Percent_Identity=28.6127167630058, Blast_Score=148, Evalue=8e-36,
Organism=Drosophila melanogaster, GI17137420, Length=354, Percent_Identity=27.4011299435028, Blast_Score=138, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24653280, Length=287, Percent_Identity=23.6933797909408, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24653276, Length=287, Percent_Identity=23.6933797909408, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24653278, Length=287, Percent_Identity=23.6933797909408, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17136286, Length=326, Percent_Identity=22.6993865030675, Blast_Score=78, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: 2.3.1.47 [C]

Molecular weight: Translated: 45846; Mature: 45846

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVLERKPDLSPKIYREPPLLPGLRERLDKQFMPHWNTQWGGKCILHGRNPGPDSIRLDG
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCEEECC
NDYLNISGHPEIVSAQIATLRRDNDSVIQSGAFLLDAHPSRRLEASMAAWIGKEDGFICQ
CCEEEECCCCCHHHHHHHHHHCCCHHHHHCCCEEEECCCCHHHHHHHHHHHCCCCCEEEC
SGYAANVGLLQAIADEKTPVYLDTLAHTSLWEGVRAARAPAHPFRHNDPAHLSRMIARNG
CCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCC
PGVVVVDSVYSTTGALCPLVEMVEVAEQHGCTILVDESHSLGTHGPQGAGLCAELGLVDR
CCEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHH
VHFITASLAKAFAGRAGFFTVPAELRYYVLHHSYPNIFSSCLLPHEIAGLAATLDVIRAS
HHHHHHHHHHHHCCCCCEEEECHHHEEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHCC
DEARERLHINTQRLRASLSDMGYPIHQGSQQIISLEAGTEPDTMVLRDRLEERQLFGAIF
HHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHEEE
CAPATSRNRAMVRLTLNAGLTASELDRIEKVAREVAPLVKPWDWPIARRARAGQAEI
ECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure
MQVLERKPDLSPKIYREPPLLPGLRERLDKQFMPHWNTQWGGKCILHGRNPGPDSIRLDG
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCEEECC
NDYLNISGHPEIVSAQIATLRRDNDSVIQSGAFLLDAHPSRRLEASMAAWIGKEDGFICQ
CCEEEECCCCCHHHHHHHHHHCCCHHHHHCCCEEEECCCCHHHHHHHHHHHCCCCCEEEC
SGYAANVGLLQAIADEKTPVYLDTLAHTSLWEGVRAARAPAHPFRHNDPAHLSRMIARNG
CCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCC
PGVVVVDSVYSTTGALCPLVEMVEVAEQHGCTILVDESHSLGTHGPQGAGLCAELGLVDR
CCEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHH
VHFITASLAKAFAGRAGFFTVPAELRYYVLHHSYPNIFSSCLLPHEIAGLAATLDVIRAS
HHHHHHHHHHHHCCCCCEEEECHHHEEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHCC
DEARERLHINTQRLRASLSDMGYPIHQGSQQIISLEAGTEPDTMVLRDRLEERQLFGAIF
HHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHEEE
CAPATSRNRAMVRLTLNAGLTASELDRIEKVAREVAPLVKPWDWPIARRARAGQAEI
ECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-Alanine; H+; Pimeloyl-CoA [C]

Specific reaction: L-Alanine + H+ + Pimeloyl-CoA <==> 8-Amino-7-oxononanoate + CO2 + Coenzyme A [C]

General reaction: Acyl group transfer [C]

Inhibitor: D/L-Histidine [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301; 12176318 [H]