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The map label for this gene is rspA [H]

Identifier: 91789818

GI number: 91789818

Start: 4170796

End: 4172007

Strand: Direct

Name: rspA [H]

Synonym: Bpro_3978

Alternate gene names: 91789818

Gene position: 4170796-4172007 (Clockwise)

Preceding gene: 91789817

Following gene: 91789839

Centisome position: 80.2

GC content: 63.28

Gene sequence:

>1212_bases
ATGAAAATCACCAACGCCCGCGTCATCGTCTGCAGCCCCGGCCGCAACTTCGTCACGCTCAAGATTGAAACCGATGAGGG
CCTGACCGGCATCGGCGACGCCACGCTCAATGGCCGCGAGCTTGCGGTGGCGAGCTACCTGACCGACCATGTGATTCCCT
GCCTGATCGGGCGGGATGCGCACCAGATTGAAGACATCTGGCAATACCTTTACAAGGGTGCGTACTGGCGGCGCGGGCCG
GTCACCATGAGCGCCATCGCCGCGGTCGATACGGCGCTGTGGGACATCAAGGCCAAGGCCGCGAACCTGCCGCTGTACCA
GCTGCTCGGCGGACGCAGCCGCACCGGGGTGATGGTCTACGGCCACGCCAATGGCAAGGACCTCGAACAAACCGTCGATG
AAGTTCTGCGCTACAAGGAAATGGGCTACCAGGCCATTCGCGCACAGAGCGGCGTGCCGGGACTGGACAAGGTCTACGGC
GTAGGCAAAGGCACGCTGTTCTACGAACCCGCGGACGCCGACCTGCCCGGCGAGCATGACTGGAGTTCGGAGAAATACCT
GCTGCACACGCCCAAACTGTTCGACGCAGTGCGCCAGGCCGTCGGGCCCGACATTCACCTGCTGCACGACGTGCACCACC
GCCTCACGCCCATCGAGGCGGCGCGGCTGGGCAAATCACTGGAGCCCTACCACCTGTTCTGGATCGAGGACGCCACACCG
GCGGAGAACCAGGAAGCGTTCAAACTGATACGCCAGCACACCACCACGCCGCTGGCGGTGGGCGAAATCTTCAACTCGCT
GTGGGACTGCAAGGACCTGATCCAGAACCAGCTGATCGACTACATTCGCGCGACGGTGGTCCACGCTGGCGGCATCACGC
ACCTGCGGCGCATTGCCGACTTTGCGGCGATGTACCAGGTGCGCACCGGCTGCCACGGCGCGACGGACCTGTCACCGGTG
TGCATGGGCACGGCCCTGCACTTTGACACCTGGGTGCCGAACTTCGGCGTGCAGGAATACATGCGGCACACCGAGGCGAC
CGACGAAGTGTTTCCGCATGCCTACAGTTTCAACAAGGGTTTCATGCACTGCGGCGAAGCTGTCGGTCACGGCGTGGAGA
TCGACGAGAAGCTGGCCGCCAAATATCCCTACCAGCGCGCCTACCTGCCGGTTAACCGGCTGCAGCATGACGGCACGTTG
TGGAACTGGTGA

Upstream 100 bases:

>100_bases
GCTCACCTGGAGCGCGTGATCAAGGAATTCGCCCAGGCCTGGCGCTAAACAGCGGCCCGGTCCCCCGCCCCGTCCATTCA
CTGAAACCCCAGCACGCATC

Downstream 100 bases:

>100_bases
ATAAGCTCACCCCTGCTGACGCTCACTGCGTGTAGCGTCCCCTCCCCTTCCAGGGGACAGGCGCCTTGGGGCCGGCAAAG
CCGGACCCCGGCCCCCACTG

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MKITNARVIVCSPGRNFVTLKIETDEGLTGIGDATLNGRELAVASYLTDHVIPCLIGRDAHQIEDIWQYLYKGAYWRRGP
VTMSAIAAVDTALWDIKAKAANLPLYQLLGGRSRTGVMVYGHANGKDLEQTVDEVLRYKEMGYQAIRAQSGVPGLDKVYG
VGKGTLFYEPADADLPGEHDWSSEKYLLHTPKLFDAVRQAVGPDIHLLHDVHHRLTPIEAARLGKSLEPYHLFWIEDATP
AENQEAFKLIRQHTTTPLAVGEIFNSLWDCKDLIQNQLIDYIRATVVHAGGITHLRRIADFAAMYQVRTGCHGATDLSPV
CMGTALHFDTWVPNFGVQEYMRHTEATDEVFPHAYSFNKGFMHCGEAVGHGVEIDEKLAAKYPYQRAYLPVNRLQHDGTL
WNW

Sequences:

>Translated_403_residues
MKITNARVIVCSPGRNFVTLKIETDEGLTGIGDATLNGRELAVASYLTDHVIPCLIGRDAHQIEDIWQYLYKGAYWRRGP
VTMSAIAAVDTALWDIKAKAANLPLYQLLGGRSRTGVMVYGHANGKDLEQTVDEVLRYKEMGYQAIRAQSGVPGLDKVYG
VGKGTLFYEPADADLPGEHDWSSEKYLLHTPKLFDAVRQAVGPDIHLLHDVHHRLTPIEAARLGKSLEPYHLFWIEDATP
AENQEAFKLIRQHTTTPLAVGEIFNSLWDCKDLIQNQLIDYIRATVVHAGGITHLRRIADFAAMYQVRTGCHGATDLSPV
CMGTALHFDTWVPNFGVQEYMRHTEATDEVFPHAYSFNKGFMHCGEAVGHGVEIDEKLAAKYPYQRAYLPVNRLQHDGTL
WNW
>Mature_403_residues
MKITNARVIVCSPGRNFVTLKIETDEGLTGIGDATLNGRELAVASYLTDHVIPCLIGRDAHQIEDIWQYLYKGAYWRRGP
VTMSAIAAVDTALWDIKAKAANLPLYQLLGGRSRTGVMVYGHANGKDLEQTVDEVLRYKEMGYQAIRAQSGVPGLDKVYG
VGKGTLFYEPADADLPGEHDWSSEKYLLHTPKLFDAVRQAVGPDIHLLHDVHHRLTPIEAARLGKSLEPYHLFWIEDATP
AENQEAFKLIRQHTTTPLAVGEIFNSLWDCKDLIQNQLIDYIRATVVHAGGITHLRRIADFAAMYQVRTGCHGATDLSPV
CMGTALHFDTWVPNFGVQEYMRHTEATDEVFPHAYSFNKGFMHCGEAVGHGVEIDEKLAAKYPYQRAYLPVNRLQHDGTL
WNW

Specific function: Probably involved in the degradation of homoserine lactone (HSL) or of a metabolite of HSL that signals starvation [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787864, Length=405, Percent_Identity=68.8888888888889, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI48994953, Length=393, Percent_Identity=31.5521628498728, Blast_Score=168, Evalue=5e-43,
Organism=Escherichia coli, GI226510960, Length=376, Percent_Identity=23.936170212766, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 45167; Mature: 45167

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITNARVIVCSPGRNFVTLKIETDEGLTGIGDATLNGRELAVASYLTDHVIPCLIGRDA
CCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCH
HQIEDIWQYLYKGAYWRRGPVTMSAIAAVDTALWDIKAKAANLPLYQLLGGRSRTGVMVY
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEE
GHANGKDLEQTVDEVLRYKEMGYQAIRAQSGVPGLDKVYGVGKGTLFYEPADADLPGEHD
ECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCEEEECCCCCCCCCCCC
WSSEKYLLHTPKLFDAVRQAVGPDIHLLHDVHHRLTPIEAARLGKSLEPYHLFWIEDATP
CCCCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEEECCCC
AENQEAFKLIRQHTTTPLAVGEIFNSLWDCKDLIQNQLIDYIRATVVHAGGITHLRRIAD
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
FAAMYQVRTGCHGATDLSPVCMGTALHFDTWVPNFGVQEYMRHTEATDEVFPHAYSFNKG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHCCCHHCCCCH
FMHCGEAVGHGVEIDEKLAAKYPYQRAYLPVNRLQHDGTLWNW
HHHHHHHHCCCCCCHHHHHHCCCCCCCCCCHHHHCCCCCCCCC
>Mature Secondary Structure
MKITNARVIVCSPGRNFVTLKIETDEGLTGIGDATLNGRELAVASYLTDHVIPCLIGRDA
CCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCH
HQIEDIWQYLYKGAYWRRGPVTMSAIAAVDTALWDIKAKAANLPLYQLLGGRSRTGVMVY
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEE
GHANGKDLEQTVDEVLRYKEMGYQAIRAQSGVPGLDKVYGVGKGTLFYEPADADLPGEHD
ECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCEEEECCCCCCCCCCCC
WSSEKYLLHTPKLFDAVRQAVGPDIHLLHDVHHRLTPIEAARLGKSLEPYHLFWIEDATP
CCCCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEEECCCC
AENQEAFKLIRQHTTTPLAVGEIFNSLWDCKDLIQNQLIDYIRATVVHAGGITHLRRIAD
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
FAAMYQVRTGCHGATDLSPVCMGTALHFDTWVPNFGVQEYMRHTEATDEVFPHAYSFNKG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHCCCHHCCCCH
FMHCGEAVGHGVEIDEKLAAKYPYQRAYLPVNRLQHDGTLWNW
HHHHHHHHCCCCCCHHHHHHCCCCCCCCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7545940; 9097039; 9278503 [H]