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The map label for this gene is truA

Identifier: 91789465

GI number: 91789465

Start: 3817322

End: 3818116

Strand: Direct

Name: truA

Synonym: Bpro_3614

Alternate gene names: 91789465

Gene position: 3817322-3818116 (Clockwise)

Preceding gene: 91789464

Following gene: 91789466

Centisome position: 73.41

GC content: 61.64

Gene sequence:

>795_bases
GTGAGGATTGCGCTCGGTATCAGCTACAGCGGCAGCGCTTATGAAGGCTGGCAAAGCCAGCTTTCAGGCAATACGGTACA
GGACAAACTGGAATCGGCATTGGCCAGGTTTGCCGCTCAGCCCGTGCGCACCCTGTGCGCCGGCCGCACCGATGCCGGCG
TTCACGGGCTGATGCAGGTCGTGCACTTTGACACCGTCCTGCACCGTGACATGGGGTCATGGGTACGCGGCACCAACGCC
TTTTTGCCTTCCGACATCGCGGTGCAGTGGGCGCGCGAAGTTCCCGATACGTTTCATTGCCGGGGCAGTGCCATTGCACG
CCGCTACGCCTACGTGGTGCTGGAATCACCCGTGCGACCCAGCGTGGAGGCCGGACGTGTCGGCTGGGTCTACCGGCCGC
TGGACGGTGCAGCGATGCGGCAGGCCATCACGCACCTGGTTGGCGAACATGACTTCAGCTCCTTTCGGGCGGCGCAATGC
CAGGCCAAATCGCCCGTCAAAACCATCAACCGGATCGAAATTTCGGAGCGTGCCGGTACCGGTTCAAAGTACTGGCACTT
CGAGTTCGAAGCCAACGCCTTTTTGCACCACATGATCCGCAACATCATGGGCTGCCTGGTCGCCATAGGTCAAGGCAGCC
ATGAGCCTGGCTGGCTGCTGGACGTGATGGCCGCACGCAGCCGGGATGCCGCCGCCCCCACCTTTTCACCCAACGGCCTC
TATTTCCTGGGGCCAGTCTATGAAGACCACTATGGCCTGCCAAATCGCACGGCGGCTTATGATTGGTTGCCATGA

Upstream 100 bases:

>100_bases
GGTCAAAGCGCAGGCGTTCCTCAACGCCCTCGCGTGATTCCGGGGTGGTATTGCAAGCCGGCCTGAACGCCGCGCGTTCA
CTGGCCGTGAAGGTTTCCCC

Downstream 100 bases:

>100_bases
CGCTCCCCCCCGTTGCTCCCCCACTCCGTGTGGGTCGCCTCCCCCCTCAAGGGGGTAACACCAGCGGCCCGGCAAAGCCG
GTTCCGCGGTGTTCCTGGAC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MRIALGISYSGSAYEGWQSQLSGNTVQDKLESALARFAAQPVRTLCAGRTDAGVHGLMQVVHFDTVLHRDMGSWVRGTNA
FLPSDIAVQWAREVPDTFHCRGSAIARRYAYVVLESPVRPSVEAGRVGWVYRPLDGAAMRQAITHLVGEHDFSSFRAAQC
QAKSPVKTINRIEISERAGTGSKYWHFEFEANAFLHHMIRNIMGCLVAIGQGSHEPGWLLDVMAARSRDAAAPTFSPNGL
YFLGPVYEDHYGLPNRTAAYDWLP

Sequences:

>Translated_264_residues
MRIALGISYSGSAYEGWQSQLSGNTVQDKLESALARFAAQPVRTLCAGRTDAGVHGLMQVVHFDTVLHRDMGSWVRGTNA
FLPSDIAVQWAREVPDTFHCRGSAIARRYAYVVLESPVRPSVEAGRVGWVYRPLDGAAMRQAITHLVGEHDFSSFRAAQC
QAKSPVKTINRIEISERAGTGSKYWHFEFEANAFLHHMIRNIMGCLVAIGQGSHEPGWLLDVMAARSRDAAAPTFSPNGL
YFLGPVYEDHYGLPNRTAAYDWLP
>Mature_264_residues
MRIALGISYSGSAYEGWQSQLSGNTVQDKLESALARFAAQPVRTLCAGRTDAGVHGLMQVVHFDTVLHRDMGSWVRGTNA
FLPSDIAVQWAREVPDTFHCRGSAIARRYAYVVLESPVRPSVEAGRVGWVYRPLDGAAMRQAITHLVGEHDFSSFRAAQC
QAKSPVKTINRIEISERAGTGSKYWHFEFEANAFLHHMIRNIMGCLVAIGQGSHEPGWLLDVMAARSRDAAAPTFSPNGL
YFLGPVYEDHYGLPNRTAAYDWLP

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=253, Percent_Identity=30.8300395256917, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1788657, Length=254, Percent_Identity=46.0629921259843, Blast_Score=239, Evalue=2e-64,
Organism=Drosophila melanogaster, GI19922728, Length=257, Percent_Identity=29.1828793774319, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_POLSJ (Q126M3)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_550417.1
- ProteinModelPortal:   Q126M3
- SMR:   Q126M3
- STRING:   Q126M3
- GeneID:   4013721
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_3614
- NMPDR:   fig|296591.1.peg.5158
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   NNYKLTI
- PhylomeDB:   Q126M3
- ProtClustDB:   PRK00021
- BioCyc:   PSP296591:BPRO_3614-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29208; Mature: 29208

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: ACT_SITE 51-51

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIALGISYSGSAYEGWQSQLSGNTVQDKLESALARFAAQPVRTLCAGRTDAGVHGLMQV
CEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
VHFDTVLHRDMGSWVRGTNAFLPSDIAVQWAREVPDTFHCRGSAIARRYAYVVLESPVRP
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHEEEEECCCCCC
SVEAGRVGWVYRPLDGAAMRQAITHLVGEHDFSSFRAAQCQAKSPVKTINRIEISERAGT
CCCCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHEEEHHCCCC
GSKYWHFEFEANAFLHHMIRNIMGCLVAIGQGSHEPGWLLDVMAARSRDAAAPTFSPNGL
CCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCE
YFLGPVYEDHYGLPNRTAAYDWLP
EEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRIALGISYSGSAYEGWQSQLSGNTVQDKLESALARFAAQPVRTLCAGRTDAGVHGLMQV
CEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
VHFDTVLHRDMGSWVRGTNAFLPSDIAVQWAREVPDTFHCRGSAIARRYAYVVLESPVRP
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHEEEEECCCCCC
SVEAGRVGWVYRPLDGAAMRQAITHLVGEHDFSSFRAAQCQAKSPVKTINRIEISERAGT
CCCCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHEEEHHCCCC
GSKYWHFEFEANAFLHHMIRNIMGCLVAIGQGSHEPGWLLDVMAARSRDAAAPTFSPNGL
CCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCE
YFLGPVYEDHYGLPNRTAAYDWLP
EEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA