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The map label for this gene is leuC [H]

Identifier: 91789459

GI number: 91789459

Start: 3809230

End: 3810651

Strand: Direct

Name: leuC [H]

Synonym: Bpro_3608

Alternate gene names: 91789459

Gene position: 3809230-3810651 (Clockwise)

Preceding gene: 91789458

Following gene: 91789460

Centisome position: 73.25

GC content: 63.5

Gene sequence:

>1422_bases
ATGGGACGCACCCTCTACGACAAAATCTGGGACGAGCACGTTGTTCACACCGAAGAAGACGGCACGTCCATTCTTTACAT
TGATCGCCACCTGGTGCATGAAGTCACCAGCCCGCAGGCCTTTGAAGGGCTGCGCGAAGCCGGCCGCAAGGTCTGGCGCA
TCAGCTCCATTGTGGCGACGGCCGACCACAACACCCCCACCACCGGCTGGGAGCTGGGCTACGACGGCATCACCGATCTG
GTCAGCAAGGAACAGATCACCACGCTGGACGCCAATATCAAGGAATTTGGCGCGGCGGCCTTCTTTCCCTTCCTGTCCAA
ACGCCAGGGCATCGTGCACGTCATCGGCCCCGAAAGCGGCGCCACCCTGCCCGGCATGACGGTGGTCTGTGGCGACTCGC
ACACCTCCACCCACGGCGCATTCGGCGCGCTGGCGCACGGCATCGGCACCAGCGAGGTCGAGCACGTGATGGCCACGCAA
ACCCTGCTGGCCAAAAAGGCCAAAAACTTGCTGATCAAGGTCGAAGGCACGCTGCCCAAAGGCTGCACCGCCAAGGACAT
CGTGCTGGCCATCATCGGCAAGATCGGCACCGCGGGCGGTACCGGCTACACCATTGAATTTGCCGGCTCGGCGATTCGCG
CGCTCAGCATGGAAGGCCGCATGACGGTCTGCAACATGGCGATTGAAGGCGGCGCCCGCGCCGGCCTGGTGGCGGTCGAC
CAGAAAACCATTGACTACGTGAAGGGCCGCCTGCTGGCGCCCACCGGTGTCGAGTGGGACCAGGCCGTGCAGTACTGGGC
CACGCTGCACTCGGATGCCGACGCGAAATTTGACGCCGTGGTCGAACTCGACGCGAGCCAGATCCTGCCGCAAGTGACTT
GGGGCACGTCCCCTGAAATGGTGCTGTCGATTGAAGACCGTGTGCCGGACCCCGACAAGGAAAAAGACGCCAACAAACGT
GGCGCCATCGAGCGTGCGCTGACTTACATGGGCCTGCAACCGGGCAAGGCGCTGAACGACCTCTACGTCGACAAGGTGTT
CATCGGCTCCTGCACCAACAGCCGCATCGAAGACATGCGCGAAGCCGCCGCCATCGTGAAAAAGCTCGGCCAGAAAGTGG
CCAAGAACGTCAAGCTGGCCATGGTGGTGCCGGGCTCCGGGCTCGTGAAGGAACAGGCTGAGCGTGAAGGCCTGGACAAG
ATTTTTGTGGCCGCCGGCTTTGAGTGGCGCGAACCCGGCTGCTCCATGTGCCTGGCCATGAATGCCGACCGGCTGGAGCC
GGGGGAGCGCTGCGCCTCCACCAGCAACCGCAACTTTGAAGGGCGTCAGGGCGCCGGCGGCCGCACCCATCTGGTCAGCC
CCGCCATGGCGGCGGCGGCGGCCGTGCATGGCCATTTTGTCGACGTGCGTAAATTTGTCTGA

Upstream 100 bases:

>100_bases
CCTGCCCGCGCCCGCCGCGAAGCCCGCAAAAACGCTTGCAAAAACCACAGTGGCAGGTGCCCGCTCACCCGCCTAAAATT
GAAAACCACGAAGGAAAACC

Downstream 100 bases:

>100_bases
ACCGAGTCACACATGCAAAAATTCACCGTACACAAGGGCCTCGTTGCCCCAATGGACCGCGAGAACGTCGACACCGACGC
CATCATCCCCAAGCAATTCC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MGRTLYDKIWDEHVVHTEEDGTSILYIDRHLVHEVTSPQAFEGLREAGRKVWRISSIVATADHNTPTTGWELGYDGITDL
VSKEQITTLDANIKEFGAAAFFPFLSKRQGIVHVIGPESGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVMATQ
TLLAKKAKNLLIKVEGTLPKGCTAKDIVLAIIGKIGTAGGTGYTIEFAGSAIRALSMEGRMTVCNMAIEGGARAGLVAVD
QKTIDYVKGRLLAPTGVEWDQAVQYWATLHSDADAKFDAVVELDASQILPQVTWGTSPEMVLSIEDRVPDPDKEKDANKR
GAIERALTYMGLQPGKALNDLYVDKVFIGSCTNSRIEDMREAAAIVKKLGQKVAKNVKLAMVVPGSGLVKEQAEREGLDK
IFVAAGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVHGHFVDVRKFV

Sequences:

>Translated_473_residues
MGRTLYDKIWDEHVVHTEEDGTSILYIDRHLVHEVTSPQAFEGLREAGRKVWRISSIVATADHNTPTTGWELGYDGITDL
VSKEQITTLDANIKEFGAAAFFPFLSKRQGIVHVIGPESGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVMATQ
TLLAKKAKNLLIKVEGTLPKGCTAKDIVLAIIGKIGTAGGTGYTIEFAGSAIRALSMEGRMTVCNMAIEGGARAGLVAVD
QKTIDYVKGRLLAPTGVEWDQAVQYWATLHSDADAKFDAVVELDASQILPQVTWGTSPEMVLSIEDRVPDPDKEKDANKR
GAIERALTYMGLQPGKALNDLYVDKVFIGSCTNSRIEDMREAAAIVKKLGQKVAKNVKLAMVVPGSGLVKEQAEREGLDK
IFVAAGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVHGHFVDVRKFV
>Mature_472_residues
GRTLYDKIWDEHVVHTEEDGTSILYIDRHLVHEVTSPQAFEGLREAGRKVWRISSIVATADHNTPTTGWELGYDGITDLV
SKEQITTLDANIKEFGAAAFFPFLSKRQGIVHVIGPESGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVMATQT
LLAKKAKNLLIKVEGTLPKGCTAKDIVLAIIGKIGTAGGTGYTIEFAGSAIRALSMEGRMTVCNMAIEGGARAGLVAVDQ
KTIDYVKGRLLAPTGVEWDQAVQYWATLHSDADAKFDAVVELDASQILPQVTWGTSPEMVLSIEDRVPDPDKEKDANKRG
AIERALTYMGLQPGKALNDLYVDKVFIGSCTNSRIEDMREAAAIVKKLGQKVAKNVKLAMVVPGSGLVKEQAEREGLDKI
FVAAGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVHGHFVDVRKFV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=368, Percent_Identity=27.9891304347826, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI8659555, Length=450, Percent_Identity=26.2222222222222, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI41352693, Length=374, Percent_Identity=25.1336898395722, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1786259, Length=472, Percent_Identity=63.9830508474576, Blast_Score=586, Evalue=1e-168,
Organism=Escherichia coli, GI1787531, Length=367, Percent_Identity=28.3378746594005, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI32564738, Length=370, Percent_Identity=30.8108108108108, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI25149337, Length=370, Percent_Identity=30.8108108108108, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI25149342, Length=318, Percent_Identity=29.559748427673, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=425, Percent_Identity=27.7647058823529, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321429, Length=474, Percent_Identity=63.0801687763713, Blast_Score=599, Evalue=1e-172,
Organism=Saccharomyces cerevisiae, GI6320440, Length=469, Percent_Identity=27.5053304904051, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6323335, Length=369, Percent_Identity=30.3523035230352, Blast_Score=136, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6322261, Length=406, Percent_Identity=28.8177339901478, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI281365315, Length=460, Percent_Identity=27.6086956521739, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI17864292, Length=460, Percent_Identity=27.6086956521739, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI161076999, Length=370, Percent_Identity=28.3783783783784, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI28571643, Length=481, Percent_Identity=25.7796257796258, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24645686, Length=443, Percent_Identity=28.4424379232506, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI17137564, Length=383, Percent_Identity=26.1096605744125, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50828; Mature: 50696

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRTLYDKIWDEHVVHTEEDGTSILYIDRHLVHEVTSPQAFEGLREAGRKVWRISSIVAT
CCCCHHHHHHHHHCEEECCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEE
ADHNTPTTGWELGYDGITDLVSKEQITTLDANIKEFGAAAFFPFLSKRQGIVHVIGPESG
CCCCCCCCCCCCCCCHHHHHHCCHHEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCC
ATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVMATQTLLAKKAKNLLIKVEGTLPK
CCCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCC
GCTAKDIVLAIIGKIGTAGGTGYTIEFAGSAIRALSMEGRMTVCNMAIEGGARAGLVAVD
CCCHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCCEEEEEEEECCCCCCCEEEEC
QKTIDYVKGRLLAPTGVEWDQAVQYWATLHSDADAKFDAVVELDASQILPQVTWGTSPEM
HHHHHHHCCEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECHHHHCCCCCCCCCCCE
VLSIEDRVPDPDKEKDANKRGAIERALTYMGLQPGKALNDLYVDKVFIGSCTNSRIEDMR
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHEEEEECCCCHHHHHHH
EAAAIVKKLGQKVAKNVKLAMVVPGSGLVKEQAEREGLDKIFVAAGFEWREPGCSMCLAM
HHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEC
NADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVHGHFVDVRKFV
CCCCCCCHHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GRTLYDKIWDEHVVHTEEDGTSILYIDRHLVHEVTSPQAFEGLREAGRKVWRISSIVAT
CCCHHHHHHHHHCEEECCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEE
ADHNTPTTGWELGYDGITDLVSKEQITTLDANIKEFGAAAFFPFLSKRQGIVHVIGPESG
CCCCCCCCCCCCCCCHHHHHHCCHHEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCC
ATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVMATQTLLAKKAKNLLIKVEGTLPK
CCCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCC
GCTAKDIVLAIIGKIGTAGGTGYTIEFAGSAIRALSMEGRMTVCNMAIEGGARAGLVAVD
CCCHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCCEEEEEEEECCCCCCCEEEEC
QKTIDYVKGRLLAPTGVEWDQAVQYWATLHSDADAKFDAVVELDASQILPQVTWGTSPEM
HHHHHHHCCEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECHHHHCCCCCCCCCCCE
VLSIEDRVPDPDKEKDANKRGAIERALTYMGLQPGKALNDLYVDKVFIGSCTNSRIEDMR
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHEEEEECCCCHHHHHHH
EAAAIVKKLGQKVAKNVKLAMVVPGSGLVKEQAEREGLDKIFVAAGFEWREPGCSMCLAM
HHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEC
NADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVHGHFVDVRKFV
CCCCCCCHHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA