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The map label for this gene is leuD [H]

Identifier: 91789460

GI number: 91789460

Start: 3810664

End: 3811314

Strand: Direct

Name: leuD [H]

Synonym: Bpro_3609

Alternate gene names: 91789460

Gene position: 3810664-3811314 (Clockwise)

Preceding gene: 91789459

Following gene: 229558816

Centisome position: 73.28

GC content: 60.98

Gene sequence:

>651_bases
ATGCAAAAATTCACCGTACACAAGGGCCTCGTTGCCCCAATGGACCGCGAGAACGTCGACACCGACGCCATCATCCCCAA
GCAATTCCTCAAATCCATCCGCAAGACCGGTTTTGGCCCCAACCTGTTTGACGCGTGGCGCTACCTGGATGCCGGCTTTC
CCGGGCAGGACCCGGCCAGCCGCAAGCCCAACCCGGACTTCGTGCTGAACCAGCCGCGCTACGCCGGTGCCTCCATCCTG
CTGGCGCGCAAGAACTTCGGCTGCGGTTCCTCGCGCGAGCATGCGCCGTGGGCGCTCGACCAGTACGGCTTTCGCGCCAT
CATCGCGCCGAGCTATGCCGACATCTTCTTCAACAACAGCTTCAAGAACGGCCTGCTGCCCATCGTGCTGCCCGAAGCCC
AGATTGCCCAGCTGTTTGACGAGGTGAACGCCTTCCCGGGCTACACGCTCACCATCGACCTGGAGCGCCAGGTCATCGTC
AAGCCCCAAGGCAAAGAGCTGCCCTTCGAAGTACAGGCTTTCCGCAAATACTGCCTGCTCAACGGTTTTGACGACATTGG
CCTGACCTTGCGCCAAAGCGACAAGATCAAGGCATTTGAAGCCGAGCGCCTCGCGAAGAAGCCCTGGCTCAACCACACCA
TCCCGGGTTGA

Upstream 100 bases:

>100_bases
CGGCGGCCGCACCCATCTGGTCAGCCCCGCCATGGCGGCGGCGGCGGCCGTGCATGGCCATTTTGTCGACGTGCGTAAAT
TTGTCTGAACCGAGTCACAC

Downstream 100 bases:

>100_bases
TAAGCCGGTATTTTTCAGTCAAATCATGCTTTTGCCCCATCAATATGGGCATGAGCAGCCATACAAAAAGATAGCAACCA
TGAAAATCGCAATTCTCCCG

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MQKFTVHKGLVAPMDRENVDTDAIIPKQFLKSIRKTGFGPNLFDAWRYLDAGFPGQDPASRKPNPDFVLNQPRYAGASIL
LARKNFGCGSSREHAPWALDQYGFRAIIAPSYADIFFNNSFKNGLLPIVLPEAQIAQLFDEVNAFPGYTLTIDLERQVIV
KPQGKELPFEVQAFRKYCLLNGFDDIGLTLRQSDKIKAFEAERLAKKPWLNHTIPG

Sequences:

>Translated_216_residues
MQKFTVHKGLVAPMDRENVDTDAIIPKQFLKSIRKTGFGPNLFDAWRYLDAGFPGQDPASRKPNPDFVLNQPRYAGASIL
LARKNFGCGSSREHAPWALDQYGFRAIIAPSYADIFFNNSFKNGLLPIVLPEAQIAQLFDEVNAFPGYTLTIDLERQVIV
KPQGKELPFEVQAFRKYCLLNGFDDIGLTLRQSDKIKAFEAERLAKKPWLNHTIPG
>Mature_216_residues
MQKFTVHKGLVAPMDRENVDTDAIIPKQFLKSIRKTGFGPNLFDAWRYLDAGFPGQDPASRKPNPDFVLNQPRYAGASIL
LARKNFGCGSSREHAPWALDQYGFRAIIAPSYADIFFNNSFKNGLLPIVLPEAQIAQLFDEVNAFPGYTLTIDLERQVIV
KPQGKELPFEVQAFRKYCLLNGFDDIGLTLRQSDKIKAFEAERLAKKPWLNHTIPG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=202, Percent_Identity=56.9306930693069, Blast_Score=238, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6321429, Length=213, Percent_Identity=48.8262910798122, Blast_Score=191, Evalue=5e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 24358; Mature: 24358

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKFTVHKGLVAPMDRENVDTDAIIPKQFLKSIRKTGFGPNLFDAWRYLDAGFPGQDPAS
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCC
RKPNPDFVLNQPRYAGASILLARKNFGCGSSREHAPWALDQYGFRAIIAPSYADIFFNNS
CCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCEEHHCCCCEEEECCCCEEEEECCC
FKNGLLPIVLPEAQIAQLFDEVNAFPGYTLTIDLERQVIVKPQGKELPFEVQAFRKYCLL
CCCCCCEEEECHHHHHHHHHHHHCCCCEEEEEEECCEEEECCCCCCCCHHHHHHHHHHHH
NGFDDIGLTLRQSDKIKAFEAERLAKKPWLNHTIPG
CCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MQKFTVHKGLVAPMDRENVDTDAIIPKQFLKSIRKTGFGPNLFDAWRYLDAGFPGQDPAS
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCC
RKPNPDFVLNQPRYAGASILLARKNFGCGSSREHAPWALDQYGFRAIIAPSYADIFFNNS
CCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCEEHHCCCCEEEECCCCEEEEECCC
FKNGLLPIVLPEAQIAQLFDEVNAFPGYTLTIDLERQVIVKPQGKELPFEVQAFRKYCLL
CCCCCCEEEECHHHHHHHHHHHHCCCCEEEEEEECCEEEECCCCCCCCHHHHHHHHHHHH
NGFDDIGLTLRQSDKIKAFEAERLAKKPWLNHTIPG
CCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA