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The map label for this gene is bcr [H]

Identifier: 91789071

GI number: 91789071

Start: 3396954

End: 3398228

Strand: Direct

Name: bcr [H]

Synonym: Bpro_3211

Alternate gene names: 91789071

Gene position: 3396954-3398228 (Clockwise)

Preceding gene: 91789070

Following gene: 91789072

Centisome position: 65.32

GC content: 66.43

Gene sequence:

>1275_bases
ATGCCCATCTCCTCCCAAAAACTGTCTTCCGAGGCTGCGCCGGCCGGCGCCGTCGTGGTACCGCCATCGATGTCGGCTGG
CCTGATCGTGCTGATCCTGTCGATGCTGCTGGGCATCCAGCCGGTCACCACCGACCTCTACCTGCCCGCGCTGCCGGCGC
TGACCGAAGGCTTTGCTGCCCCGATGTCGCAGGCGCAGCTCACGCTGAGTGCGCTGCTGCTGGCTTTCGGCGTCTCCCAG
CTGATCTGGGGCCCGCTGTCCGACCGCTTTGGCCGGCGTCCCATCCTGTTGTGGGGCCTGGCCACTTACACGCTGGCCTC
GATCGGCAGCACGCTGGCCCCCTCCATGGCGCTGCTGATCGTCTGGCGCATTGTGCAGGGCGCGGCCATGGGCGCGGTCG
TGATGTGCGCACGCGCCATCGTGCGCGACCTGTACCACCCGGTTGAAGGCGCCCGCATCATGTCCAAGGGCCTGAGCGGC
CTGGGCGTTCTGGCCTGCATCAGTGCCCCGCTGGGCGGCTTGCTGTCGGAGTTTTGGGGCTGGCGCGTCGCGCTGATGGC
CCTGGCCGTATTTGGCGGCTGCACCCTGGCGCTGGTCGCGCTGCGTTTTGAAGAAACCCTGCGGCAGAAAAACCCGCAGG
CGCTGCATCCGGCCACGCTGCTGCGCACCTGGATCCATATCCTCGGCAATCCCACCTTCCTGGTGTTCTCGGCGCTGGCC
GCGGCGTCGTATGGCGGGCTGTTTACCTTTCTCGCCACGTCGTCGTTTGTGTTCATCAAGGTGCTGGGGCTGAGCAAAAC
ACAGTACGGCCTGGTGATGTTTTCCATGTGCTTTTGCTACCTGACGGGCACCTTTATCTGCCGCCGCCTGCTGCCGCGTT
TTGGCGTGCGCAAGTCGGTGGCGATTGCTGCAGGCCTGAGCGTTGCCGGCGGCACGCTGATGGGCATCTTCGCCTGGGCC
GGCCTGAGCAACGTCTGGGCCATCATGCTGCCCTACTACCTCTTCATGCTGGCCCACGGCGTGCACCAGCCCTGCGGCCA
GAGCGGTGCGGTGGGGCCCTTTCCGCACGCGGCAGGCGCGGCCTCGGCCATGAACGGCTTCCTGATGATGGTCCTGGCCT
TTGGCATGGGCGGCTGGCTGGGCACGCAAATGGATGGCACCGTCCTGCCGCTGACCAACGGCGTATGGTTCTGGAGCGTG
CTGGTGGCCGCCACCGCCTGGACCGTGGTGCAGAAATACGGAGAGCCTGCCCATGAGGCCGCCACCAAACCCTGA

Upstream 100 bases:

>100_bases
CGCTGGCGCAGCTTTTCCAGCTGAAATAAGTCAGCCGCGCGCCCAGGCTGCCGTGCCTTTGCCACGCCTTGGTGGCGGGC
AATCTGATACCCTCGAACGG

Downstream 100 bases:

>100_bases
CGTGAAGCCGGAGACAAGCGTGGGAAGGCAATTTTCCGCCGGGCCGCCCCAAGGAAAATTAGCCCCCTCGGGGCTGGAGC
CTGCGGCTCCAAGTCTGCTT

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MPISSQKLSSEAAPAGAVVVPPSMSAGLIVLILSMLLGIQPVTTDLYLPALPALTEGFAAPMSQAQLTLSALLLAFGVSQ
LIWGPLSDRFGRRPILLWGLATYTLASIGSTLAPSMALLIVWRIVQGAAMGAVVMCARAIVRDLYHPVEGARIMSKGLSG
LGVLACISAPLGGLLSEFWGWRVALMALAVFGGCTLALVALRFEETLRQKNPQALHPATLLRTWIHILGNPTFLVFSALA
AASYGGLFTFLATSSFVFIKVLGLSKTQYGLVMFSMCFCYLTGTFICRRLLPRFGVRKSVAIAAGLSVAGGTLMGIFAWA
GLSNVWAIMLPYYLFMLAHGVHQPCGQSGAVGPFPHAAGAASAMNGFLMMVLAFGMGGWLGTQMDGTVLPLTNGVWFWSV
LVAATAWTVVQKYGEPAHEAATKP

Sequences:

>Translated_424_residues
MPISSQKLSSEAAPAGAVVVPPSMSAGLIVLILSMLLGIQPVTTDLYLPALPALTEGFAAPMSQAQLTLSALLLAFGVSQ
LIWGPLSDRFGRRPILLWGLATYTLASIGSTLAPSMALLIVWRIVQGAAMGAVVMCARAIVRDLYHPVEGARIMSKGLSG
LGVLACISAPLGGLLSEFWGWRVALMALAVFGGCTLALVALRFEETLRQKNPQALHPATLLRTWIHILGNPTFLVFSALA
AASYGGLFTFLATSSFVFIKVLGLSKTQYGLVMFSMCFCYLTGTFICRRLLPRFGVRKSVAIAAGLSVAGGTLMGIFAWA
GLSNVWAIMLPYYLFMLAHGVHQPCGQSGAVGPFPHAAGAASAMNGFLMMVLAFGMGGWLGTQMDGTVLPLTNGVWFWSV
LVAATAWTVVQKYGEPAHEAATKP
>Mature_423_residues
PISSQKLSSEAAPAGAVVVPPSMSAGLIVLILSMLLGIQPVTTDLYLPALPALTEGFAAPMSQAQLTLSALLLAFGVSQL
IWGPLSDRFGRRPILLWGLATYTLASIGSTLAPSMALLIVWRIVQGAAMGAVVMCARAIVRDLYHPVEGARIMSKGLSGL
GVLACISAPLGGLLSEFWGWRVALMALAVFGGCTLALVALRFEETLRQKNPQALHPATLLRTWIHILGNPTFLVFSALAA
ASYGGLFTFLATSSFVFIKVLGLSKTQYGLVMFSMCFCYLTGTFICRRLLPRFGVRKSVAIAAGLSVAGGTLMGIFAWAG
LSNVWAIMLPYYLFMLAHGVHQPCGQSGAVGPFPHAAGAASAMNGFLMMVLAFGMGGWLGTQMDGTVLPLTNGVWFWSVL
VAATAWTVVQKYGEPAHEAATKP

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=381, Percent_Identity=27.5590551181102, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI87082312, Length=323, Percent_Identity=30.6501547987616, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI48994889, Length=335, Percent_Identity=29.2537313432836, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1790146, Length=332, Percent_Identity=27.1084337349398, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1790794, Length=231, Percent_Identity=28.1385281385281, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787880, Length=176, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319517, Length=217, Percent_Identity=28.110599078341, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6324264, Length=164, Percent_Identity=31.0975609756098, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322071, Length=165, Percent_Identity=32.1212121212121, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44771; Mature: 44640

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPISSQKLSSEAAPAGAVVVPPSMSAGLIVLILSMLLGIQPVTTDLYLPALPALTEGFAA
CCCCCHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
PMSQAQLTLSALLLAFGVSQLIWGPLSDRFGRRPILLWGLATYTLASIGSTLAPSMALLI
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
VWRIVQGAAMGAVVMCARAIVRDLYHPVEGARIMSKGLSGLGVLACISAPLGGLLSEFWG
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
WRVALMALAVFGGCTLALVALRFEETLRQKNPQALHPATLLRTWIHILGNPTFLVFSALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
AASYGGLFTFLATSSFVFIKVLGLSKTQYGLVMFSMCFCYLTGTFICRRLLPRFGVRKSV
HHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
AIAAGLSVAGGTLMGIFAWAGLSNVWAIMLPYYLFMLAHGVHQPCGQSGAVGPFPHAAGA
HHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
ASAMNGFLMMVLAFGMGGWLGTQMDGTVLPLTNGVWFWSVLVAATAWTVVQKYGEPAHEA
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHC
ATKP
CCCC
>Mature Secondary Structure 
PISSQKLSSEAAPAGAVVVPPSMSAGLIVLILSMLLGIQPVTTDLYLPALPALTEGFAA
CCCCHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
PMSQAQLTLSALLLAFGVSQLIWGPLSDRFGRRPILLWGLATYTLASIGSTLAPSMALLI
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
VWRIVQGAAMGAVVMCARAIVRDLYHPVEGARIMSKGLSGLGVLACISAPLGGLLSEFWG
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
WRVALMALAVFGGCTLALVALRFEETLRQKNPQALHPATLLRTWIHILGNPTFLVFSALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
AASYGGLFTFLATSSFVFIKVLGLSKTQYGLVMFSMCFCYLTGTFICRRLLPRFGVRKSV
HHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
AIAAGLSVAGGTLMGIFAWAGLSNVWAIMLPYYLFMLAHGVHQPCGQSGAVGPFPHAAGA
HHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
ASAMNGFLMMVLAFGMGGWLGTQMDGTVLPLTNGVWFWSVLVAATAWTVVQKYGEPAHEA
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHC
ATKP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]